BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,229,079	T→C	196 (TAA→TAG)	yohC ←	Yip1 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,229,079	0	T	C	99.4%	1026.7 / NA	310	196 (TAA→TAG)	yohC	Yip1 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (109/198); minor base G (1/1); total (110/200)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGA  >  NZ_CP025268/2228943‑2229214

cgcACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAg                                                                                                                                      >  1:304682/1‑140 (MQ=255)

  cagcgccgGAGTAAGGATTTACTGAGGCGAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGaa                                                                                                                                    <  2:4688025/137‑1 (MQ=255)

  cACCGCCTGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGaa                                                                                                                                    <  2:5204380/140‑1 (MQ=255)

  cACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGaa                                                                                                                                    <  1:1278397/140‑1 (MQ=255)

     cgccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGACCTTTCTAGAaaaa                                                                                                                                 >  2:20340/1‑140 (MQ=255)

      gccgGAGTAAGGTTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCACAGGAGCATCGAGCGGCAACGCTGCATTGCTGACATTTACGCCAGCAACGCACCGTGAAGGCACTTTCTAGAACaaa                                                                                                                                >  2:4555932/1‑140 (MQ=255)

         ggAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGa                                                                                                                             <  1:437750/140‑1 (MQ=255)

          gAGTAAGGATTTACTGCGGCTAGCGACGCTATCATAACGAGCAAACAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCCTTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACCCTCTAGAACACACGAt                                                                                                                            <  2:2593681/140‑1 (MQ=255)

          gAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGAt                                                                                                                            <  1:1745267/140‑1 (MQ=255)

          gAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGAt                                                                                                                            <  1:1625938/140‑1 (MQ=255)

          gAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGAt                                                                                                                            <  1:2119721/140‑1 (MQ=255)

          gAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGAt                                                                                                                            <  2:3321543/140‑1 (MQ=255)

          gAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGAt                                                                                                                            <  2:3046726/140‑1 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCGAAAAGTGCGATAGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACTAACGATAACCg                                                                                                                       <  2:1041249/140‑1 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       >  1:4737133/1‑140 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       >  1:3431333/1‑140 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAAACg                                                                                                                       >  2:5403346/1‑140 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  2:1548996/140‑1 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  1:1065844/140‑1 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  1:1304020/140‑1 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCAGAACGAGCAAAAACTGCGATCTTCAAAGGAAAATCGTGAGGAAATGCGGCATTGCTGACATTTAAGCCAGCAACGCAACGTCAAAGACCTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  2:2330082/140‑1 (MQ=255)

                    ttACTGAGGCTAGCGCCGCAATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGcc                                                                                                                  <  2:117909/140‑1 (MQ=255)

                    ttACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGcc                                                                                                                  <  1:1191640/140‑1 (MQ=255)

                    ttACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGcc                                                                                                                  <  1:5208206/140‑1 (MQ=255)

                    ttACTGAGGCCAGCGGCGCCACGATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGGGAGGAAATGCTACATTGCTGACATTTACGGCAGCAATGCAACGTCATAGAACTTTCTAGTCCAAACAATAACCGTAGcc                                                                                                                  <  2:5520998/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 >  1:280545/1‑140 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 <  1:1758924/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 <  2:4166415/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 <  1:5347393/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 >  2:5462460/1‑140 (MQ=255)

                       ttGACGCTACTGACGTCATCATAACGAGCAAAAATTTCGAACGTCAAAGGCGCATCGCGAGGAAATGCTGCATTGCTGGCATTTACGCCAGCGATGCAACGTAAAAGATCTTTATAGCAGGAACGATAGCCGTAGCCCCa                                                                                                               <  2:3263537/139‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:4029334/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:3785962/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:4312587/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:2612819/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:2282456/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:648802/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:5542365/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:1033456/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:5461964/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:3571696/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:5061538/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:2461242/140‑1 (MQ=255)

                            gCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGta                                                                                                          >  2:2792480/1‑140 (MQ=255)

                             ttAGAGACGCCATCAGAACGAACAAAAAGTTCGATCGTAATAGTAGAACCCTCACCAAATGCTGCATTGCGGACATTTTCGGCAGCAATGCAACGTCAAAAGACACTGTATAATTATCGATAACCGTAGCCCCATAGtat                                                                                                         <  2:797992/139‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  1:4550080/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTGACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  2:428794/140‑1 (MQ=255)

                               aGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAAACGTAAGGAAATGCTGCATAGCGGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGCATaa                                                                                                       >  2:341478/1‑140 (MQ=255)

                                 cGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATGGTATAACg                                                                                                     >  1:2324073/1‑140 (MQ=255)

                                    cGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGtt                                                                                                  <  1:2704656/140‑1 (MQ=255)

                                      ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAg                                                                                                >  1:5154622/1‑140 (MQ=255)

                                      ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGCTAg                                                                                                >  2:3433346/1‑140 (MQ=255)

                                       catcatAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAgg                                                                                               <  1:3328727/140‑1 (MQ=255)

                                           atAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGTCATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAACCGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGa                                                                                           <  2:3758399/140‑1 (MQ=255)

                                               cGAGCAAAATGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATATCGGTTAGGGCGagcag                                                                                       <  2:5248014/140‑1 (MQ=255)

                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGCACGAACGATAACCGTAGCCCGAGAGTATAACGGTTAGGGCGAGCAGCACt                                                                                   >  2:720859/1‑140 (MQ=255)

                                                            cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTAcc                                                                          >  2:1348276/1‑140 (MQ=255)

                                                                cGTCAAAGTAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCAGAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                      <  2:605583/140‑1 (MQ=255)

                                                                      aGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCaa                                                                >  2:3140415/1‑140 (MQ=255)

                                                                          gaATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTCTACCGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGc                                                            <  2:1211520/140‑1 (MQ=255)

                                                                            aTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCa                                                          >  1:46319/1‑140 (MQ=255)

                                                                                tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTcc                                                                                >  2:4830371/1‑114 (MQ=255)

                                                                                tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTcc                                                                                <  1:4830371/114‑1 (MQ=255)

                                                                                tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGTCAATCGCCAGCGt                                                      <  2:144326/140‑1 (MQ=255)

                                                                                tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                      <  1:1349980/140‑1 (MQ=255)

                                                                                tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                      <  2:1305591/140‑1 (MQ=255)

                                                                                tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                      <  1:1101068/140‑1 (MQ=255)

                                                                                       aTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGCACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTa                                               >  2:3843378/1‑140 (MQ=255)

                                                                                       aTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTa                                               >  1:945303/1‑140 (MQ=255)

                                                                                          ttgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                            >  1:4354475/2‑140 (MQ=255)

                                                                                          ctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                            <  2:918450/140‑1 (MQ=255)

                                                                                            gcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGaa                                          >  1:1991941/1‑140 (MQ=255)

                                                                                              aTTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGc                                        >  2:4500445/1‑140 (MQ=255)

                                                                                               ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCt                                       >  1:771359/1‑140 (MQ=255)

                                                                                               ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCt                                       <  1:3246626/140‑1 (MQ=255)

                                                                                                tGCCGACATTTACGACAGCAATGCAACGTCAAAGACCTTTCCAGCACCCACGATCACCGTAGCCCCAAAGTATAACGGTTAGGGCGAGCAGCACTTCCAGAACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTa                                      <  2:433661/140‑1 (MQ=255)

                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAg                                    >  2:2208064/1‑140 (MQ=255)

                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAg                                    >  1:2430138/1‑140 (MQ=255)

                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAg                                    >  1:602848/1‑140 (MQ=255)

                                                                                                       aTTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCtt                               >  1:3161037/1‑140 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctct                          >  1:3272535/1‑140 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctct                          >  1:2554083/1‑140 (MQ=255)

                                                                                                               cAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttg                       >  2:1782018/1‑140 (MQ=255)

                                                                                                                ggCAATGCAAGCTCAACGATCTTTCTAGAACAAGCGATAACCGTAGCCCCACTGTATAACGGATACGGCGACCACCAATTCCAGCACCAGCACGCTCGTCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt                      <  2:1927216/139‑1 (MQ=255)

                                                                                                                aGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt                      <  2:3523825/140‑1 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     >  2:3877460/1‑140 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     >  1:29314/1‑140 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGGTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     >  1:3774091/1‑140 (MQ=255)

                                                                                                                  cAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgttg                    >  1:4154349/1‑140 (MQ=255)

                                                                                                                    aTGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGat                  >  2:1905463/1‑140 (MQ=255)

                                                                                                                       cAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATAtt               >  2:1620294/1‑140 (MQ=255)

                                                                                                                          cGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAg            >  1:4984065/1‑140 (MQ=255)

                                                                                                                           gTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAgg           <  1:3679036/140‑1 (MQ=255)

                                                                                                                           gTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAgg           <  2:3655884/140‑1 (MQ=255)

                                                                                                                            tCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAg                                                                        <  1:5429301/78‑1 (MQ=255)

                                                                                                                             cAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGaa         <  2:2430138/140‑1 (MQ=255)

                                                                                                                              aaaGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAg        <  1:2332730/140‑1 (MQ=255)

                                                                                                                                aGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCt      <  2:5005851/140‑1 (MQ=255)

                                                                                                                                aGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCt      <  1:4459150/140‑1 (MQ=255)

                                                                                                                                   aCTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCgg   <  1:4781974/140‑1 (MQ=255)

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGA  >  NZ_CP025268/2228943‑2229214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`