BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,383,383	T→C	432 (TAA→TAG)	menF ←	isochorismate synthase MenF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,383,383	0	T	C	100.0%	873.5 / NA	262	432 (TAA→TAG)	menF	isochorismate synthase MenF
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (178/84); total (178/84)

Only 100 of 267 total aligned reads displayed.

TCCAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAG  >  NZ_CP025268/2383248‑2383518

 ccAGAATGACCGCCGCCCAGCGTCGGTTAACTGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTc                                                                                                                                    >  2:709239/1‑140 (MQ=255)

 ccAGAATGACCGCCGCCCAGCGCCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTc                                                                                                                                    >  2:738951/1‑140 (MQ=255)

    gAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCAt                                                                                                                                 <  1:1041/140‑1 (MQ=255)

    gAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCAt                                                                                                                                 <  1:2763567/140‑1 (MQ=255)

    gAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCAt                                                                                                                                 <  1:1578086/140‑1 (MQ=255)

    gAAGGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCAt                                                                                                                                 <  1:998310/140‑1 (MQ=255)

       tGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTg                                                                                                                              >  1:3566667/1‑140 (MQ=255)

         aCCGCCGCCCGGCGTCGGTTAAATGCGCTTACTGACATGATTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGta                                                                                                                            <  2:2717579/140‑1 (MQ=255)

           cgccgcCCCGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATACGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaat                                                                                                                          >  2:1643746/1‑140 (MQ=255)

             ccgccCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaataa                                                                                                                        <  1:675358/140‑1 (MQ=255)

               gccCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGAATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAg                                                                                                                      >  1:4083219/1‑140 (MQ=255)

                    gCGTCGGTTAAATGCGCTTCCGCACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTAGAAGGAGCTACACAAATAGCTTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGc                                                                                                                 <  2:2011211/140‑1 (MQ=255)

                    gCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGc                                                                                                                 >  1:4155100/1‑140 (MQ=255)

                     cGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCa                                                                                                                >  1:1920686/1‑140 (MQ=255)

                     cGTCGGTTAAATGCGCTTAATGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGCTTATTGATATGAACCGGTAATGATGCGACTCATTACTATTCCATTTTTAATAAAGTACGCa                                                                                                                >  2:2159220/1‑140 (MQ=255)

                                   gcTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttg                                                                                                  <  2:1206137/140‑1 (MQ=255)

                                    cTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttgg                                                                                                 >  1:4949458/1‑139 (MQ=255)

                                               gAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTcc                                                                                      >  1:734688/1‑140 (MQ=255)

                                                 gTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTg                                                                                    >  2:1873286/1‑140 (MQ=255)

                                                   ttGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGcc                                                                                  <  2:4319109/140‑1 (MQ=255)

                                                    tGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCa                                                                                 >  1:1443478/1‑140 (MQ=255)

                                                      tCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCAct                                                                               >  1:1366766/1‑140 (MQ=255)

                                                          gTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTg                                                                           >  2:5410889/1‑140 (MQ=255)

                                                           tATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGc                                                                                   >  2:1291227/1‑131 (MQ=255)

                                                           tATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGc                                                                          >  1:3071326/1‑140 (MQ=255)

                                                             tCAATATTGCGGTTAAGTATAAGGAGCGACAAAAATAAATTATTGATATGAATCGGTAATGATGCGACTCACTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                        <  2:5083830/140‑1 (MQ=255)

                                                             tCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                        <  2:1417175/140‑1 (MQ=255)

                                                             tCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAACCGGCGATGACGCCACTCAGTACATTGTCAGTTGCGACAAAGGAGGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCGTGCTc                                                                        <  2:4881588/140‑1 (MQ=255)

                                                                    tGCGGCTAAGTATAAGGGGCTACAAAAATAGTGTATTGACATGTATCGGTAAGGTTTCGACTCATGACTATTCTATTTGTAATAAAGTACTCAGCCCTGCCGCTTTGTTGTCGATTTCCTTCCACTCTTGCTCGGGGTCg                                                                 <  2:2771339/140‑1 (MQ=255)

                                                                    tGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                 <  1:602322/140‑1 (MQ=255)

                                                                     gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCgg                                                                >  1:1330156/1‑140 (MQ=255)

                                                                     gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCgg                                                                >  2:1266820/1‑140 (MQ=255)

                                                                     gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCgg                                                                >  2:3870283/1‑140 (MQ=255)

                                                                     gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCgg                                                                >  2:1049882/1‑140 (MQ=255)

                                                                       ggCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGaa                                                              >  2:171731/1‑140 (MQ=255)

                                                                        gCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAAc                                                             <  1:2085364/140‑1 (MQ=255)

                                                                         cTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCTGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAAcc                                                            >  2:2902766/1‑140 (MQ=255)

                                                                           aaGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGTTGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCAc                                                          >  2:4441644/1‑140 (MQ=255)

                                                                           aaGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCAc                                                          >  2:4126257/1‑140 (MQ=255)

                                                                               ataAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGAc                                                      <  1:2222824/140‑1 (MQ=255)

                                                                                   ggAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATg                                                  >  1:401967/1‑140 (MQ=255)

                                                                                     aGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGcc                                                <  2:1175691/140‑1 (MQ=255)

                                                                                      gCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGccc                                               >  1:2693938/1‑140 (MQ=255)

                                                                                      gCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGccc                                               >  1:2389585/1‑140 (MQ=255)

                                                                                      gCTACAAAAATAGATTATTGATATGAATCGGTAATGAGACGACTCATTACGATTCAATTTGTAATAAAGGACGCAGCCCTGCCGCGTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAAGCACGGACACTGccc                                               >  2:2349353/1‑140 (MQ=255)

                                                                                       cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTGCATTTGGAATAAAGTACGCAGCCATGCCGCGTTGTCGTCGTTTGCGTGCCACTGTTGCTCGGGGTCGGAGCCACGGGGAAGGCCcg                                              >  2:4507858/1‑140 (MQ=255)

                                                                                       cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                              >  2:247752/1‑140 (MQ=255)

                                                                                       cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                              >  1:361611/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACg                                                                                                                  >  1:2852071/1‑71 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCGCTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:5512176/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGTAACCACGGACAATGCCcgc                                             >  2:3131569/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:5202818/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:2272972/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:2649703/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:570179/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:3672420/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:3298685/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:1059367/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:915343/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:2310740/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:2532835/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:2240829/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:2068740/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:4815602/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:1682752/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:5299958/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  1:534856/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACAACGGACAATGCCcgc                                             >  2:3032153/1‑140 (MQ=255)

                                                                                        tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGATTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                             >  2:4202887/1‑140 (MQ=255)

                                                                                          cAAACATAGATTATTGATATGAATCGGTAATGATGCGACTCAGTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                           <  2:1356088/140‑1 (MQ=255)

                                                                                          cAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                           <  1:5171195/140‑1 (MQ=255)

                                                                                          cAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                           <  2:4722355/140‑1 (MQ=255)

                                                                                          cAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                           <  1:3265412/140‑1 (MQ=255)

                                                                                               aTAGATTATTGATATGAATCGGTAATGATGCGACGAATTACTATTCCATTTGTAATAACGTACGCAGCCCTGCCGGTTTGTTGTCGATTTCCTGCCACTCTTGCTCGAGATCGGAACCACGGACCATGACCGGGCCAGCa                                      >  2:4626745/1‑140 (MQ=255)

                                                                                                  gATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCatat                                   <  1:112279/140‑1 (MQ=255)

                                                                                                  gATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCatat                                   <  1:3034390/140‑1 (MQ=255)

                                                                                                         gATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCa                            >  1:2966180/1‑140 (MQ=255)

                                                                                                          atatGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCAc                           >  1:1758277/1‑140 (MQ=255)

                                                                                                            atGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGa                         >  1:3498146/1‑140 (MQ=255)

                                                                                                             tGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGAc                        >  2:2092249/1‑140 (MQ=255)

                                                                                                                 tCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACCTTg                    >  2:304876/1‑140 (MQ=255)

                                                                                                                    gTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                        >  2:2872612/1‑85 (MQ=255)

                                                                                                                    gTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGTACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCg                 >  1:1980444/1‑140 (MQ=255)

                                                                                                                    gTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCg                 >  1:5128393/1‑140 (MQ=255)

                                                                                                                    gTAATGATGCGACTCATTACTATTCCATTTGAAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCg                 >  2:4284266/1‑140 (MQ=255)

                                                                                                                      aaTGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCt               <  1:2423056/140‑1 (MQ=255)

                                                                                                                      aaTGATGCGACCCATTACTAATCCATTTGTAATCAAGTAAGCCAACCTGCCGCTTTATTGTCCCTTCCCTGCCACTCATCCTCGGCGTCAGAACCACGGACAATGCCCGCACCAGCATCTAATCGAACGACATTGCCgat               <  2:337872/140‑3 (MQ=255)

                                                                                                                          atgCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAtt           >  1:2154161/1‑140 (MQ=255)

                                                                                                                          atgCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGAGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAtt           >  1:4667524/1‑140 (MQ=255)

                                                                                                                             cGACTCATTACTATTCCATTTGTAATAAAGTCCGCAGCCCTGCAGCACTCTTGTCGATTTACAGCCACACTTGCACGGGGTCGGACCCACGCATAATGCCCGGGCCACCCTATCTTTGCACGACATTGCCGCtgccgttt        >  2:819112/1‑133 (MQ=255)

                                                                                                                              gACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg       >  1:4272206/1‑140 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                           <  2:5507914/103‑1 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGc      >  2:2948349/1‑140 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGc      >  1:3871565/1‑140 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGc      >  1:4380110/1‑140 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACAGTGCCGCTAATTTTTGc      >  2:1739947/1‑140 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGCTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGc      >  2:4937388/1‑140 (MQ=255)

                                                                                                                               aCTCATTACTATTCCATTTGTAAGAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGc      >  1:1954309/1‑140 (MQ=255)

                                                                                                                                cTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCt     <  1:3156533/140‑1 (MQ=255)

                                                                                                                                   attactattCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAg  >  1:5016059/1‑140 (MQ=255)

TCCAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGAG  >  NZ_CP025268/2383248‑2383518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`