BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,457,300	T→C	pseudogene (1737/2646 nt)	yfcU ←	fimbrial usher protein YfcU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,457,300	0	T	C	99.4%	1545.9 / NA	466	pseudogene (1737/2646 nt)	yfcU	fimbrial usher protein YfcU
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (217/245); minor base A (1/2); total (219/247)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 466 total aligned reads displayed.

GTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAG  >  NZ_CP025268/2457163‑2457435

gTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTa                                                                                                                                       >  1:1409543/1‑140 (MQ=255)

 tGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTAc                                                                                                                                      <  1:5252724/140‑1 (MQ=255)

 tGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTAc                                                                                                                                      <  1:1527749/140‑1 (MQ=255)

 tGCCGATGTTCAACTGATAGTGAGTCGCGTCACCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTAc                                                                                                                                      <  2:3578269/140‑1 (MQ=255)

  gCCGATGTTCAACTGATCGTGAGTCGCGGCATCGACACGGCTAAACTACCCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGTTAACGGTACTCTTTTCTCCCCTCGGCATAATTAGCGATATCTACa                                                                                                                                     <  2:269453/140‑1 (MQ=255)

  gCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACa                                                                                                                                     <  1:3327685/140‑1 (MQ=255)

  gCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACa                                                                                                                                     <  1:592697/140‑1 (MQ=255)

  gCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACa                                                                                                                                     <  2:3826355/140‑1 (MQ=255)

   ccGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACAt                                                                                                                                    >  1:5409058/1‑140 (MQ=255)

   ccGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACAt                                                                                                                                    >  2:1244853/1‑140 (MQ=255)

   ccGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACAt                                                                                                                                    <  2:2438024/140‑1 (MQ=255)

      aTGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGcc                                                                                                                                 <  2:3441242/140‑1 (MQ=255)

          tCAACTGATAGTGAGGCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACGTCCATAGTTGCCGTTAGAGCTAACGGTACTGTTGTCGCCCCACTGCATACTTAGCGATATCTACAGTCCCTTa                                                                                                                             >  2:2589867/1‑140 (MQ=255)

           cAACTGATAGTGAGTCGCGTCATCGACACGGCTCAAATAACCGACCTCGCGGCTGTCCGTCCCACTGCCATAGTTGCCGGTATAGCTCACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTAt                                                                                                                            >  2:694532/1‑140 (MQ=255)

               tGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGc                                                                                                                        <  1:1015234/140‑1 (MQ=255)

                gATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGcc                                                                                                                       <  1:3140653/140‑1 (MQ=255)

                 aTAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGccc                                                                                                                      >  1:5443608/1‑140 (MQ=255)

                 aTAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGccc                                                                                                                      >  1:2605191/1‑140 (MQ=255)

                 aTAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGGCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGccc                                                                                                                      <  2:2036289/140‑1 (MQ=255)

                   aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCgg                                                                                                                    <  2:4694454/140‑1 (MQ=255)

                   aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCgg                                                                                                                    <  1:1627640/140‑1 (MQ=255)

                   aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCgg                                                                                                                    >  1:1890477/1‑140 (MQ=255)

                   aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCgg                                                                                                                    <  2:29296/140‑1 (MQ=255)

                   aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCgg                                                                                                                    >  2:1905897/1‑140 (MQ=255)

                   aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCgg                                                                                                                    <  1:357663/140‑1 (MQ=255)

                     tGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGtt                                                                                                                  >  2:3224315/1‑140 (MQ=255)

                      gAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTg                                                                                                                 >  1:2192984/1‑140 (MQ=255)

                       aGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGt                                                                                                                >  1:1225792/1‑140 (MQ=255)

                       aGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGt                                                                                                                >  1:649636/1‑140 (MQ=255)

                        gTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGGTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTc                                                                                                               >  1:4540311/1‑140 (MQ=255)

                         tCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCa                                                                                                              >  1:2549520/1‑140 (MQ=255)

                         tCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCa                                                                                                              >  1:2731967/1‑140 (MQ=255)

                            cgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCACCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATAc                                                                                                           >  1:3981698/1‑140 (MQ=255)

                            cgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGGTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGCCATAc                                                                                                           >  2:1734318/1‑140 (MQ=255)

                                   gACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGc                                                                                                    <  1:781399/140‑1 (MQ=255)

                                        ggCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAg                                                                                               <  1:891540/140‑1 (MQ=255)

                                        ggCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAg                                                                                               >  1:4903926/1‑140 (MQ=255)

                                        ggCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAg                                                                                               <  1:513245/140‑1 (MQ=255)

                                        agCTAACATCACCGACCCGGCTGCTGCCCGTCCCCCTCCCATAGTTCCCGCTATAGCTAACGGTACTGCCGTCGCCCCACGGCATACTGCGCGAAATCTACATGCCCTTATGCTCCCGGTTGTCATACTCGTAGCGGTAg                                                                                               <  2:1613795/139‑1 (MQ=255)

                                                  aCCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAggg                                                                                     >  1:4610397/1‑140 (MQ=255)

                                                  aCCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAggg                                                                                     >  2:516938/1‑140 (MQ=255)

                                                   cccaCCTCGCTGCTGTCCGTCCCACTGCCATCGTTGCCGTTCTAGCTAACGCTACTGTTCTCGCCCCACGGCATACTGCGCGCAATCTACCTGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                    <  2:3320331/137‑1 (MQ=255)

                                                   ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                    >  2:4183084/1‑140 (MQ=255)

                                                   ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                    <  2:1691626/140‑1 (MQ=255)

                                                   ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                    <  1:3020392/140‑1 (MQ=255)

                                                    cGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGaa                                                                                   >  1:266573/1‑140 (MQ=255)

                                                    cGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGaa                                                                                   >  2:4578008/1‑140 (MQ=255)

                                                         tGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                              <  2:2610151/140‑1 (MQ=255)

                                                          ggCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTc                                                                             <  1:3479445/140‑1 (MQ=255)

                                                            ctgctgTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCat                                                                           <  1:2910635/140‑1 (MQ=255)

                                                               ctgTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATAtt                                                                        >  2:229333/1‑140 (MQ=255)

                                                                 gtccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTAc                                                                      >  1:3766647/1‑140 (MQ=255)

                                                                  tccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACg                                                                     >  1:298498/1‑140 (MQ=255)

                                                                     gtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAAt                                                                  >  1:4404852/1‑140 (MQ=255)

                                                                     gtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAAt                                                                  >  2:5274988/1‑140 (MQ=255)

                                                                           cTGCAATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAAGGCTGcc                                                            <  1:2059189/140‑1 (MQ=255)

                                                                            tGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGccc                                                           >  1:479099/1‑140 (MQ=255)

                                                                              ccATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCat                                                         >  1:1970814/1‑140 (MQ=255)

                                                                              ccATAGTGGCCGTTATAGCTAACGGTACTGGTGTCGCCCCACGGCATACTGAGCGAAATCTACCTGCGCTTATCCGCACGGGTGTCATACTCGTAGAGGTAGCCAGTCAGGCAAACGCGCATAGTCCCGATGGTGcccgt                                                         >  2:4047077/1‑138 (MQ=255)

                                                                                aTAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGGCAGTCAGGGAAACGCTCATATTACGAATGCGGCTCatat                                                       >  1:1105127/1‑140 (MQ=255)

                                                                                aTAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCatat                                                       >  1:41047/1‑140 (MQ=255)

                                                                                 tAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATAtt                                                      <  2:832857/140‑1 (MQ=255)

                                                                                   gTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGa                                                    <  2:3742919/140‑1 (MQ=255)

                                                                                   gTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGa                                                    <  1:368289/140‑1 (MQ=255)

                                                                                     tGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGaaa                                                  <  1:5503204/140‑1 (MQ=255)

                                                                                        cGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAg                                               >  1:4204506/1‑140 (MQ=255)

                                                                                           tataGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCACGGTTGTCATACTCGTAGCGGTAGCAAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTgg                                            <  1:182292/140‑1 (MQ=255)

                                                                                             taGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGga                                          <  1:2319297/140‑1 (MQ=255)

                                                                                                  aaCGGTACTGTTGTCGCCCCGCGGCATACTGATCGACATCTACATGCCCTTATCCGCCAGGTTGGCATACTCGTAGCGGGAGCCAGTCAGGGAATCGCTCATGTTACGAATGCTGTCCAAATTGAACTAGTGGGAGAGCa                                     >  2:2758480/1‑140 (MQ=255)

                                                                                                  aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGCAACGCTCATAGTACGAATGCTGCCCCTATTGAAATAGTGGGAgcgcc                                     >  2:5598385/1‑139 (MQ=255)

                                                                                                  aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCa                                     <  1:1425399/140‑1 (MQ=255)

                                                                                                  aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCa                                     >  2:4491139/1‑140 (MQ=255)

                                                                                                  aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCa                                     <  2:4901881/140‑1 (MQ=255)

                                                                                                  aaCGGTACTGTCGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCa                                     <  1:247500/140‑1 (MQ=255)

                                                                                                    cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCatg                                   >  1:2107801/1‑140 (MQ=255)

                                                                                                    cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCatg                                   <  1:3394419/140‑1 (MQ=255)

                                                                                                    cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCGGCCCATATTGAAATAGTGGGAGAGCatg                                   >  2:5432851/1‑140 (MQ=255)

                                                                                                        aCTGTTGTCGCCCCACGGCATACTGAGCGCAATCTACATGCCCTTATCCGCCCGTTTGTCATACTCGGAGCGGTAGCCAGTCAGTGAAACGCTCATATTACGAATGCTCCCCATATTGAAATAGTGGGAGAGCATGAtgt                               >  2:1201363/1‑140 (MQ=255)

                                                                                                        aCTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGAtgt                               <  2:5542808/140‑1 (MQ=255)

                                                                                                         cTGTTGTCGACCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCCGGGAAACGCTCATATTCCGAATGCTGCCCATATTGAAATAGTGGTAGAGCATGAtgtt                              <  2:1790208/140‑1 (MQ=255)

                                                                                                              gtCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGCAGCCAGTCAGAGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGAGAGAGCATGATGTTGTAGt                         >  2:5060841/1‑140 (MQ=255)

                                                                                                               tCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGtt                        >  2:2395497/1‑140 (MQ=255)

                                                                                                                 gCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGTAGAGCATGATGTTGTAGTTTg                      >  2:3710478/1‑140 (MQ=255)

                                                                                                                 gCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTg                      >  2:4197368/1‑140 (MQ=255)

                                                                                                                 gCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGCTGTCATACTCGTAGCGGTAGCCAGTCAGGGCAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTCTg                      >  2:4356254/1‑140 (MQ=255)

                                                                                                                      aCGGCATCCTGCGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTATCGGTAGCCGTTCAGTGAAACGCTCATATGACGAATGCGGCCCATAGTGGAATAGTAGGATGGCAGGATGTTGTAATTTGTCTGc                 >  2:316085/1‑140 (MQ=255)

                                                                                                                             attGAGCGAAAGCTACATGCCCTTACCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCAc          <  2:5390338/138‑1 (MQ=255)

                                                                                                                             aCTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGa                                                                    >  1:1708570/1‑82 (MQ=255)

                                                                                                                             aCTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCAc          >  1:148075/1‑140 (MQ=255)

                                                                                                                             aCTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTAAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTACTAAc          <  2:1367525/140‑1 (MQ=255)

                                                                                                                              cTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACg         <  2:1454950/140‑1 (MQ=255)

                                                                                                                               tGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGa        <  2:601419/140‑1 (MQ=255)

                                                                                                                               tGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGa        <  2:4987411/140‑1 (MQ=255)

                                                                                                                                gAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGAt       <  2:3528143/140‑1 (MQ=255)

                                                                                                                                caGCCAAATCTACATGCCCTTATCCGCCCCGCTGTCATAATCGTAGCGGTACCCACTCAGGGAAACGATCCTATTACGAATGCTGCCCATATTGCAATAGTGGCAGACCATGATGTTGTAGTTTGTCTGCTCCTCACGAt       <  2:3052725/139‑1 (MQ=255)

                                                                                                                                 aGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGCTGTTGTAGTTTGTCTGCTCCTCACGATc      >  2:2141002/1‑140 (MQ=255)

                                                                                                                                 aGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATc      >  1:987184/1‑140 (MQ=255)

                                                                                                                                 aGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATc      >  1:5583850/1‑140 (MQ=255)

                                                                                                                                     aaaTCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAg  <  1:1269567/140‑1 (MQ=255)

                                                                                                                                     aaaTCTACATGCCCTTATCCGCCCGGGTGTCATACGCGTACCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTCCCCATATTGAAAGGGTGGGAGAGCATGATGTTGTAGTTTGTCTGCGCCTCACGATCCCAg  <  2:148868/140‑1 (MQ=255)

GTGCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCCAG  >  NZ_CP025268/2457163‑2457435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`