BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,529,956	T→C	309 (TAA→TAG)	yfeR ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,529,956	0	T	C	100.0%	915.2 / NA	275	309 (TAA→TAG)	yfeR	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (154/121); total (154/121)

Only 100 of 275 total aligned reads displayed.

ATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCA  >  NZ_CP025268/2529824‑2530091

tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                  >  1:3508187‑M2/129‑140 (MQ=255)

tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                  >  1:2318563‑M2/129‑140 (MQ=255)

  ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                                >  2:4695658‑M2/127‑140 (MQ=255)

  ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                                >  1:2477354‑M2/127‑140 (MQ=255)

   tacggggattcggtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGAt                                                                                                                               >  2:3986159‑M2/126‑140 (MQ=255)

   tacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGAt                                                                                                                               >  1:3570663‑M2/126‑140 (MQ=255)

   tacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGAt                                                                                                                               >  2:1186367‑M2/126‑140 (MQ=255)

     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             <  1:3148451‑M2/17‑1 (MQ=255)

     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             <  1:1425244‑M2/17‑1 (MQ=255)

     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:1608690‑M2/124‑140 (MQ=255)

     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:640204‑M2/124‑140 (MQ=255)

     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:5359478‑M2/124‑140 (MQ=255)

     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:220822‑M2/124‑140 (MQ=255)

       gggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACaa                                                                                                                           >  1:74692‑M2/122‑140 (MQ=255)

        ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  2:1042304‑M2/121‑140 (MQ=255)

        ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaatgCACTCTATATCTGATACAAc                                                                                                                          >  1:3078597‑M2/121‑140 (MQ=255)

         gattcagtatcgatatgcggatgacaaactgggcgaagatgtttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACg                                                                                                                         <  1:4006106‑M2/21‑1 (MQ=255)

          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgcttgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  2:4058447‑M2/119‑140 (MQ=255)

          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:3684126‑M2/119‑140 (MQ=255)

          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:1306634‑M2/119‑140 (MQ=255)

          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:1136769‑M2/119‑140 (MQ=255)

          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  2:4765332‑M2/119‑140 (MQ=255)

          attcagtatcgatatgcggatgacaaactgggcgaagacttttaccaggatgcgatcatcgactccatcaactttcacttctgcttgattactgccggggctctcggcagcacgaattCACTCTATATCTGATACAACgg                                                                                                                        >  2:824292‑M2/119‑140 (MQ=255)

             cagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATc                                                                                                                     >  1:2832086‑M2/116‑140 (MQ=255)

               gtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATccc                                                                                                                   >  1:5146147‑M2/114‑140 (MQ=255)

                tatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATcccc                                                                                                                  >  1:4092891‑M2/113‑140 (MQ=255)

                   cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTc                                                                                                               <  2:4882727‑M2/31‑1 (MQ=255)

                        tgcggatgacaagctgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          <  1:4785668‑M2/36‑1 (MQ=255)

                         gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                         <  1:4277490‑M2/37‑1 (MQ=255)

                         gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                         >  1:3246848‑M2/104‑140 (MQ=255)

                          cggatgacaaactgggcgaagatttttaccaggatgcgataatctactcgatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        >  2:4248429‑M2/103‑140 (MQ=255)

                          cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        <  2:301397‑M2/38‑1 (MQ=255)

                          cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        <  1:212923‑M2/38‑1 (MQ=255)

                               gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                                   <  2:2495335‑M2/43‑1 (MQ=255)

                                atcaactgggcgatgatttttacctggatgcgatattctacttcttcaaatttatcttctgattgattactgccggggctcccggcagtttggattCACTCTATATCTGCGTCAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                                  <  2:2812196‑M2/44‑1 (MQ=255)

                                acaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                                  <  2:1343716‑M2/44‑1 (MQ=255)

                                      tggtcaaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggcttccggcagtatgatttCACTCTATATCTGATACAACGGATCCCCTTCCCGCCTAGCCATTAGCGCa                                                                                            <  2:445860‑M2/50‑1 (MQ=255)

                                      tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:1751586‑M2/50‑1 (MQ=255)

                                      tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:4492514‑M2/50‑1 (MQ=255)

                                      tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:1878641‑M2/50‑1 (MQ=255)

                                      tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:3388960‑M2/50‑1 (MQ=255)

                                       gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                           <  2:5562842‑M2/51‑1 (MQ=255)

                                            agggtttttaccaggatgcgataatgtactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  2:3541743‑M2/56‑1 (MQ=255)

                                            aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCGGCCATTAGCGCATTGccc                                                                                      <  1:4211807‑M2/56‑1 (MQ=255)

                                            aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  1:4931639‑M2/56‑1 (MQ=255)

                                            aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  1:1042304‑M2/56‑1 (MQ=255)

                                             agatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                                     <  1:469462‑M2/57‑1 (MQ=255)

                                              gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                    <  2:5100282‑M2/58‑1 (MQ=255)

                                              gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                    <  1:2733580‑M2/58‑1 (MQ=255)

                                              gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                    <  1:1673054‑M2/58‑1 (MQ=255)

                                                 ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                                                 <  2:518063‑M2/61‑1 (MQ=255)

                                                 ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                                                 <  2:2666909‑M2/61‑1 (MQ=255)

                                                   ttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                               <  1:2042541‑M2/63‑1 (MQ=255)

                                                       caggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAc                                                                           <  1:1186367‑M2/67‑1 (MQ=255)

                                                           atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGTTCTCGTACCACg                                                                       >  1:5433224‑M2/70‑140 (MQ=255)

                                                           atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                       <  2:1463365‑M2/71‑1 (MQ=255)

                                                            tgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGt                                                                      <  2:801111‑M2/72‑1 (MQ=255)

                                                             gcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTc                                                                     >  1:4845448‑M2/68‑140 (MQ=255)

                                                                    tctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGGCCAGCCATTAGCGCATTGCCCTGGTCTCATACCACGTCCCATaaa                                                              >  2:1694617‑M2/61‑140 (MQ=255)

                                                                    tctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCGCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                              >  2:377937‑M2/61‑140 (MQ=255)

                                                                    tctactccatcaaaattaacttctgattgatgactgcctgggctcccggcagtatgtattCACTCTATATCTGAGACAACGGATCAGCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                              >  2:3325216‑M2/61‑140 (MQ=255)

                                                                    gctactccatcaaatttaacttctgattgattactgccggggctcccggccgtatgaattCACTCTATATCTAATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                              >  2:5289252‑M2/61‑140 (MQ=255)

                                                                      tcctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGc                                                            <  1:2933048‑M2/82‑1 (MQ=255)

                                                                        ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                          >  1:3741424‑M2/57‑140 (MQ=255)

                                                                        ctccatcaaatttaacttctgagtgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                          >  1:4511552‑M2/57‑140 (MQ=255)

                                                                           catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATCCAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTCCCACGTCCCATAAAGCTTCAg                                                       >  2:674411‑M2/54‑140 (MQ=255)

                                                                           catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                       >  2:1384504‑M2/54‑140 (MQ=255)

                                                                             tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCagca                                                     <  1:1666991‑M2/89‑2 (MQ=255)

                                                                             tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     <  1:1073578‑M2/89‑1 (MQ=255)

                                                                             tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     >  1:5307091‑M2/52‑140 (MQ=255)

                                                                             tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     <  2:3927279‑M2/89‑1 (MQ=255)

                                                                              caaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                                    >  2:31668‑M2/51‑140 (MQ=255)

                                                                               aaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc                                                   >  2:2887789‑M2/50‑140 (MQ=255)

                                                                                aatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                                  <  1:5550861‑M2/92‑1 (MQ=255)

                                                                                    taacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                              <  1:5097434‑M2/96‑1 (MQ=255)

                                                                                     aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                             >  1:3015295‑M2/44‑140 (MQ=255)

                                                                                     aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                             <  2:2706919‑M2/97‑1 (MQ=255)

                                                                                       cttccgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTTCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCAGTGGaaa                                           >  2:5149351‑M2/42‑140 (MQ=255)

                                                                                          ctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAACGCTTCAGCGGCGGTGGAAAGTg                                        <  2:3395883‑M2/102‑1 (MQ=255)

                                                                                          ctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                                        <  2:2069490‑M2/102‑1 (MQ=255)

                                                                                           tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGCTACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       <  2:2030294‑M2/103‑1 (MQ=255)

                                                                                                  ttactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttc                                >  1:778110‑M2/31‑140 (MQ=255)

                                                                                                     ctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                             <  1:3328678‑M2/113‑1 (MQ=255)

                                                                                                     ctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                             <  1:4182452‑M2/113‑1 (MQ=255)

                                                                                                       gccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                           >  1:1967019‑M2/26‑140 (MQ=255)

                                                                                                        ccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                          <  1:2409651‑M2/116‑1 (MQ=255)

                                                                                                        ccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGAGCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                          <  1:2334085‑M2/116‑1 (MQ=255)

                                                                                                        ccggggcccccggccgtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCctc                          >  2:4214849‑M2/25‑139 (MQ=255)

                                                                                                           gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:2574753‑M2/22‑140 (MQ=255)

                                                                                                           gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:1097914‑M2/22‑140 (MQ=255)

                                                                                                            ggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  2:1636126‑M2/21‑37 (MQ=255)

                                                                                                            ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                      >  2:580193‑M2/21‑140 (MQ=255)

                                                                                                            ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                      >  1:4794902‑M2/21‑140 (MQ=255)

                                                                                                               tcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa                   <  1:3918172‑M2/123‑1 (MQ=255)

                                                                                                                cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCATCGGCGGTGGAAAGTGAACGGTTGTTCCTGCGCACAAg                  >  2:4347428‑M2/17‑140 (MQ=255)

                                                                                                                cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg                  >  1:2541448‑M2/17‑140 (MQ=255)

                                                                                                                 ccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGc                 <  2:3054930‑M2/125‑1 (MQ=255)

                                                                                                                  cggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa                >  1:1505003‑M2/15‑140 (MQ=255)

                                                                                                                    gcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATc              <  2:5437134‑M2/128‑1 (MQ=255)

                                                                                                                               tCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTc   >  2:5403612‑M2/2‑140 (MQ=255)

ATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCA  >  NZ_CP025268/2529824‑2530091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`