BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,654,514	T→C	M600V (ATG→GTG)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,654,514	0	T	C	98.8%	1116.6 / ‑6.5	340	M600V (ATG→GTG)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (173/161); minor base G (4/0); total (177/163)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

TTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGT  >  NZ_CP025268/2654378‑2654649

     gacTTCATCCACACTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACTGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCCCCGGGCGAc                                                                                                                                 <  1:3394773/138‑1 (MQ=255)

        ttCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTGCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGGCAGGTAGGCTGCTCAGCGCGGCGCGTCACCGGGCGACCGc                                                                                                                              >  1:1915009/1‑140 (MQ=255)

        ttCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGc                                                                                                                              >  1:3711828/1‑140 (MQ=255)

        ttCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGc                                                                                                                              >  1:355577/1‑140 (MQ=255)

          catcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                            >  1:4277087/1‑140 (MQ=255)

          catcaACAATGGGATGTTTCACCGTGCTGTCAGTACGATAATCGTAAACGGATTTCTAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGCCACCGGGCGACCGccc                                                                                                                            >  2:2852465/1‑140 (MQ=255)

           atcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCGCCGGGCGACCGCCCg                                                                                                                           >  1:2683909/1‑140 (MQ=255)

               acaaTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTc                                                                                                                       <  1:2036153/140‑1 (MQ=255)

                  aTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTGCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCCACCGCCCGATGCCAg                                                                                                                    >  2:165732/1‑140 (MQ=255)

                   tGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGc                                                                                                                   >  2:5032928/1‑140 (MQ=255)

                   tGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGc                                                                                                                   >  2:5030372/1‑140 (MQ=255)

                     ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCCGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                 >  2:3707696/1‑140 (MQ=255)

                      gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                <  1:1160085/140‑1 (MQ=255)

                      gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                <  2:13965/140‑1 (MQ=255)

                      gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                <  2:3120946/140‑1 (MQ=255)

                      gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                <  1:1555092/140‑1 (MQ=255)

                          tttCACCGTGCTGTCCGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                            >  1:1714054/1‑140 (MQ=255)

                          tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACGGCCCGATTCCAGCAAACTAc                                                                                                            >  2:3421385/1‑140 (MQ=255)

                          tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                            >  1:408867/1‑140 (MQ=255)

                          tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                            >  2:120795/1‑140 (MQ=255)

                          tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                            >  2:5237865/1‑140 (MQ=255)

                           ttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAcc                                                                                                           <  1:791151/140‑1 (MQ=255)

                           ttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAcc                                                                                                           >  1:1603964/1‑140 (MQ=255)

                                 gTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaa                                                                                                     >  1:2075554/1‑140 (MQ=255)

                                  tGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaa                                                                                                    >  1:3007573/1‑140 (MQ=255)

                                  tGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaa                                                                                                    <  2:5518955/140‑1 (MQ=255)

                                    cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                  >  1:2423813/1‑140 (MQ=255)

                                    cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                  <  2:3181172/140‑1 (MQ=255)

                                    cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                  >  1:52505/1‑140 (MQ=255)

                                     tGTCAGTACGATAATCATAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATTCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCTCCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATa                                                                                                 <  1:3101559/140‑1 (MQ=255)

                                       tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                               <  2:5165861/140‑1 (MQ=255)

                                       tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                               <  1:2807953/140‑1 (MQ=255)

                                       tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                               <  1:2394/140‑1 (MQ=255)

                                       tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTCCTGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                               <  2:1621302/140‑1 (MQ=255)

                                       tCAGCACGATAATACTAAACCAATCTCGCGGCCACATCCGGACGGATCCCCGGCAATGCCCCGGAAGGCCACATAGCATGCTCAGCCCGGCGCGTCACCGAGCTACCGCCCCATCCCAGCAAACTCCCCTGGAAAATAAc                                                                                               <  2:4525019/140‑1 (MQ=255)

                                          gTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATCGATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCAGCGGGCGACCGCCCGATTCCAGCAAACTACCCGGGAAAATAACCTg                                                                                            >  2:4843401/1‑140 (MQ=255)

                                            aCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCACACTACCCTGGAAAATAACCTGTa                                                                                          <  2:2477564/140‑1 (MQ=255)

                                            aCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTa                                                                                          >  2:851549/1‑140 (MQ=255)

                                            aCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTa                                                                                          <  1:1191919/140‑1 (MQ=255)

                                                 aaTCGTAAACCGATTTCGAGGCGAACTGCTGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTTCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAGTggg                                                                                     >  2:4267117/1‑140 (MQ=255)

                                                 aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATAGGCAGGCGAGATAGCCTGCTAAGCGCGGCGCGTCACCGCGCGACCGCCCGATTCCAGCAAACTACTCTGGAAAATAACCTTGAATggg                                                                                     >  2:2563327/1‑140 (MQ=255)

                                                   tcatcaaCAGATTTAGACGCGAACTGCGGACACATGCCAGGCAATGCATCGGCAGGCCAGATAACATGCTCAGCGGCGCGGGTTAGCGGGCGACGGGCCGATTGCAGCAAAGTACCTGGGAAAATGACCTATAAggagga                                                                                   >  2:2994059/6‑140 (MQ=255)

                                                   tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                   >  1:2116204/1‑140 (MQ=255)

                                                   tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                   <  1:5122370/140‑1 (MQ=255)

                                                     gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCGTCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCGGTAATGGGGAAt                                                                                 >  1:4780921/1‑140 (MQ=255)

                                                     gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                 >  2:1491295/1‑140 (MQ=255)

                                                            gATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttc                                                                          <  1:2464036/140‑1 (MQ=255)

                                                            gATTTCGAGGCGAAATGCGGACGGATCCCCGGCACAGCATCGGCAGGCCAAATAACCTGCTCAGCGAGGCGCGGCACCGGGCGACCGCCCGATTCCAGCCAGCTACCCTGGAAAATAACCTGTAATGGGGAATGCGttgc                                                                          <  2:2774336/140‑3 (MQ=255)

                                                                tCGAGGCGAAGTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttccttc                                                                      <  2:1134834/140‑1 (MQ=255)

                                                                tCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttccttc                                                                      <  1:3131267/140‑1 (MQ=255)

                                                                   aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCAc                                                                   >  1:594053/1‑140 (MQ=255)

                                                                        aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                              <  2:3325516/140‑1 (MQ=255)

                                                                        aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                              >  1:3573788/1‑140 (MQ=255)

                                                                        aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                              <  2:4700825/140‑1 (MQ=255)

                                                                            gCGGACGCATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCACACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCt                                                          <  2:1091212/140‑1 (MQ=255)

                                                                             cggacggaTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTg                                                         >  1:2505555/1‑140 (MQ=255)

                                                                                     tCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACt                                                 <  1:3827556/140‑1 (MQ=255)

                                                                                          ggCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgcc                                            <  1:2190358/140‑1 (MQ=255)

                                                                                           gCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                           <  1:1399940/140‑1 (MQ=255)

                                                                                           gCAATGAATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                           <  1:366277/140‑1 (MQ=255)

                                                                                             aatgactCGGCAGGCCAGATAGCCTGCTCAGCGCGACGCGTCACCGAGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                         <  1:2912599/134‑1 (MQ=255)

                                                                                             aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                         <  2:4354163/140‑1 (MQ=255)

                                                                                             aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                         <  1:5326430/140‑1 (MQ=255)

                                                                                             aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                         <  2:4855216/140‑1 (MQ=255)

                                                                                              aTGCATCGGCCGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAGCCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAACCTTCGCCgcgc                                        <  2:1858622/140‑1 (MQ=255)

                                                                                              aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                        >  1:4243131/1‑140 (MQ=255)

                                                                                              aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                        >  2:2275802/1‑140 (MQ=255)

                                                                                              aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                        <  1:3589364/140‑1 (MQ=255)

                                                                                               tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                       <  1:1829577/140‑1 (MQ=255)

                                                                                               tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                       <  2:807095/140‑1 (MQ=255)

                                                                                               tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                       >  2:3825025/1‑140 (MQ=255)

                                                                                               tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                       <  1:1272369/140‑1 (MQ=255)

                                                                                               tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                       >  1:3887068/1‑140 (MQ=255)

                                                                                               tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGCAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                       <  2:2630746/140‑1 (MQ=255)

                                                                                                 cATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCt                                     <  2:645539/140‑1 (MQ=255)

                                                                                                   tCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCttt                                   <  2:4077716/140‑1 (MQ=255)

                                                                                                    cGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAGCTTCGCCGCGCGCTTTa                                  <  2:1313388/140‑1 (MQ=255)

                                                                                                         ggCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcatc                             <  1:5030372/140‑1 (MQ=255)

                                                                                                         ggCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcatc                             <  1:5032928/140‑1 (MQ=255)

                                                                                                         ggCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcatc                             >  1:5428764/1‑140 (MQ=255)

                                                                                                          gCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATcc                            >  1:655906/1‑140 (MQ=255)

                                                                                                            cAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAg                          >  1:2250126/1‑140 (MQ=255)

                                                                                                               aTAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGt                       <  2:355577/140‑1 (MQ=255)

                                                                                                                    cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                  <  2:3274319/140‑1 (MQ=255)

                                                                                                                    cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                  <  1:4195205/140‑1 (MQ=255)

                                                                                                                    cTGCGCAGCGCGGCGCGTCAACTGGCGACCGCCCGATTCCAGCAAACTACCGTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGTCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                  <  2:3599591/140‑1 (MQ=255)

                                                                                                                       cTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGCATGCGTTACCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGaa               >  2:5373348/1‑140 (MQ=255)

                                                                                                                        tCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGAGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAAc              >  1:4366672/1‑140 (MQ=255)

                                                                                                                            cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa          >  2:5601239/1‑140 (MQ=255)

                                                                                                                            cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa          >  1:4540686/1‑140 (MQ=255)

                                                                                                                            cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa          >  2:2197118/1‑140 (MQ=255)

                                                                                                                            cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa          >  1:3045528/1‑140 (MQ=255)

                                                                                                                             gcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCAt         >  1:4844532/1‑140 (MQ=255)

                                                                                                                             gcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCAt         >  2:3033777/1‑140 (MQ=255)

                                                                                                                              cggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATc        <  1:1446927/140‑1 (MQ=255)

                                                                                                                                 cgcgTCAGCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATCACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAg     >  2:4427637/1‑140 (MQ=255)

                                                                                                                                 cgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAg     >  1:3024700/1‑140 (MQ=255)

                                                                                                                                 cgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAg     >  1:4694134/1‑140 (MQ=255)

                                                                                                                                   cgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCAGCCAGCg   <  1:2296080/140‑1 (MQ=255)

                                                                                                                                    gTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGt  >  1:911327/1‑140 (MQ=255)

TTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGT  >  NZ_CP025268/2654378‑2654649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`