BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,659,791	C→T	A198T (GCT→ACT)	pepB ←	aminopeptidase PepB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,659,791	0	C	T	99.3%	1590.1 / NA	436	A198T (GCT→ACT)	pepB	aminopeptidase PepB
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (191/240); minor base G (3/0); total (196/240)
Fisher's exact test for biased strand distribution p-value = 8.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 436 total aligned reads displayed.

CAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGC  >  NZ_CP025268/2659654‑2659927

cAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGa                                                                                                                                        >  2:4866084/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAGTGCCAGCGCCCCGGTAATCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATGAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:4841848/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:1016472/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:2008573/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      <  1:3534303/140‑1 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:1219806/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGAAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:5133742/1‑140 (MQ=255)

   cTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATAc                                                                                                                                     <  1:4411286/140‑1 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                <  2:5569170/140‑1 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                >  1:1873971/1‑140 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                <  1:2587857/140‑1 (MQ=255)

            cgtGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTtaccta                                                                                                                            <  1:3282372/138‑1 (MQ=255)

               gTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCa                                                                                                                         <  1:4694264/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATCCCTTTACCTACCAgg                                                                                                                       <  2:2258234/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  1:250247/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:3326602/140‑1 (MQ=255)

                 gtaGTCCGCGCGGCATGGCAAATGCCAGCGCCCGGGTCACGGTGGGCGCCCGGCCGATGTCCGACTTCACCGAGGTCAGATTCTCAGTCTGTTTGCTGCGGCCGCAGCCGGCGTCAAAAGTCAGACCTTGACCTTCCTgg                                                                                                                       <  2:4777983/138‑1 (MQ=255)

                 ccAGTCCGAGCGTAATGGCACATGACAGCGCCCCGGTAACCGTTCCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCCCTCTGTTTAATGCTGTAGCCGCCGCAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:2128902/139‑1 (MQ=255)

                       cgcgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAcgcg                                                                                                                 <  1:4117499/140‑1 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               >  1:797625/1‑140 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               >  1:592893/1‑140 (MQ=255)

                          gcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTac                                                                                                              <  1:4019702/140‑1 (MQ=255)

                               aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGCTTGATGCTGTAGCCGCCGGCGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTgg                                                                                                         >  1:1205186/1‑140 (MQ=255)

                                 gacAAACGCCAGCGCCCCGGTAACCGTTCCCTTGCCACGCTTGGCCGACTACATAGAGTCAATAAGCACAGTCAGTGTGGTGCTGTAGCCGCCGGTGTAAAAAGTAATTCCTGTACCATCCACCCACGCCTACCCGGgcg                                                                                                       <  2:5502788/138‑1 (MQ=255)

                                   cAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGct                                                                                                     >  1:4222016/1‑140 (MQ=255)

                                      aTGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGcttct                                                                                                  >  1:2921109/1‑140 (MQ=255)

                                        gCCAGCGCCACGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCttt                                                                                                <  2:575395/140‑1 (MQ=255)

                                          cAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTAt                                                                                              <  1:2455085/140‑1 (MQ=255)

                                          cAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTAt                                                                                              >  1:3887992/1‑140 (MQ=255)

                                            gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCg                                                                                            >  2:3896258/1‑140 (MQ=255)

                                            gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCg                                                                                            >  1:746361/1‑140 (MQ=255)

                                            gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCg                                                                                            >  1:2879649/1‑140 (MQ=255)

                                            gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGAGCTTCTTTATCg                                                                                            >  2:2681491/1‑140 (MQ=255)

                                                  cGGTAACCGTTGCCGCGCCGCCCAGGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                                      <  1:5491475/140‑1 (MQ=255)

                                                   ggTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                                     <  2:3482937/140‑1 (MQ=255)

                                                   ggTAACCGTTGCCGCGCCGCCCATGACCGACTTCATCGAGTCCATAAACGCAGTCTGTATGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                                     <  2:5052993/140‑1 (MQ=255)

                                                     tAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTCCCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTggg                                                                                   <  2:3675603/140‑1 (MQ=255)

                                                     tAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCTCTTCTTTATCGCCAGTTggg                                                                                   <  2:5227371/140‑1 (MQ=255)

                                                             gccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                           >  2:1839967/1‑140 (MQ=255)

                                                                 cgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgc                                                                       >  2:2517032/1‑140 (MQ=255)

                                                                   ccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcgc                                                                     >  2:2205826/1‑140 (MQ=255)

                                                                   ccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGACGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcgc                                                                     >  2:4945139/1‑140 (MQ=255)

                                                                    cgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                                    <  1:4209484/140‑1 (MQ=255)

                                                                    cgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                                    <  1:2704613/140‑1 (MQ=255)

                                                                      cccATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                                  <  1:4850593/140‑1 (MQ=255)

                                                                      cccATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                                  <  1:4510271/140‑1 (MQ=255)

                                                                      cccATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                                  <  1:5200623/140‑1 (MQ=255)

                                                                         ctGTCCGACCTCATCGAGCCCATCACCGCAGTCTGTTTGATGCTGTAGCCTCCGGAGTCAAAAGTGATACCCTTACCTAGCAGGCACGCGTACACTGGCGCTTCTTTATAGCCAGTTGGGTTGTAATCCAGCGCCAGCaa                                                               <  2:1712997/139‑1 (MQ=255)

                                                                             ccGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATAcc                                                           <  1:3837213/140‑1 (MQ=255)

                                                                                  ttCATCGAGTCCATAACCGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  2:1280814/140‑1 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACTCAGTCTGTTTGATGCTGTATCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCGTCTTTATCGCCAGTTGGGTTTTAATCCAGCGCCAGCAATACCGgaga                                                      <  2:712092/140‑1 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  1:1159335/140‑1 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  2:3777451/140‑1 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  1:5338972/140‑1 (MQ=255)

                                                                                    cATCGATTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCa                                                                                                                         <  2:3998398/71‑1 (MQ=255)

                                                                                     aTCGAGTCCATAAACGCAGTAGGTTTGATGCTGTGGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTAGCGCCGGTTGGGTTGTTAGTCAGCTCCAGCAGCACCGGAGAACg                                                   >  2:3558866/1‑140 (MQ=255)

                                                                                      tCGGGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGGCAAAAGTGCTACCTTTACCTACCACTCACGCGTACACTGGCCCTTCTTTATCCCCCGTTGGGGTGTAATCCGTCGCCAGCAATACCGAGGAACgg                                                  >  2:4553338/1‑139 (MQ=255)

                                                                                      tCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGt                                                  >  2:4327673/1‑140 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            >  1:1047940/1‑140 (MQ=255)

                                                                                                 aaCGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACcgcg                                       <  1:4339729/140‑1 (MQ=255)

                                                                                                   cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTc                                     <  2:1670196/140‑1 (MQ=255)

                                                                                                   cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTc                                     <  1:4945220/140‑1 (MQ=255)

                                                                                                      aGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGa                                  <  2:150953/140‑1 (MQ=255)

                                                                                                       gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGCACCGCGTCCGAc                                 >  2:3963091/1‑140 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:4052423/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  2:895164/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:1367202/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:4435964/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                >  1:465347/1‑140 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:5414771/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  2:2384851/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  1:2157482/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  1:890757/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  1:3198721/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTaca                                                                                                             >  2:4417775/1‑55 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:4593064/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:3184254/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:3415440/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:5335316/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:5505579/140‑1 (MQ=255)

                                                                                                                aTGCAGTAGTCGTCGCACTCAAAAGTGACCACTTCCCCTACCAGTCACGCCTAAGCTGGCGCTTCTCTATCGCCAATTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:3003941/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTGAACAGTGATCCCTTTACCTACCAGGCACGCGTACACTGGCGCTTCGTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  2:1366559/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:143413/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:1231500/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:1648823/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:3063233/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  2:5133742/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:5056351/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:1918185/140‑1 (MQ=255)

                                                                                                                  gcagTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTCCCAGGCCCGCGTACACTGGCGCTTCTTTAACGCCAGTTGGGTTGTAGTCCAGCGCCAGGAATACCCGAGAACGTTCTGAACCGCGTCCGACTGTGTgccgcc                      <  2:1891292/137‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      >  2:2790265/1‑140 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  1:3698095/140‑1 (MQ=255)

                                                                                                                    tGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcccc                    >  1:68385/1‑140 (MQ=255)

                                                                                                                      tAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCat                  >  2:5275605/1‑140 (MQ=255)

                                                                                                                      tAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCat                  >  1:5556688/1‑140 (MQ=255)

                                                                                                                      tAGCCGCCCGAGCCAAAAGTTATACCTGGACCGACCAGGCGCGCGTACACTGGCGCTGCTTTATAGCCAGATGTGACGTAGTCGAGCGCCAGCAATACCGGAAAACGTTCTGAACCGCGTCCGCCTGTATGCAGCCCCat                  >  2:4652665/1‑140 (MQ=255)

                                                                                                                           gccgGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAAc             <  2:4212571/140‑1 (MQ=255)

                                                                                                                             cgGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCt           >  1:2380061/1‑140 (MQ=255)

                                                                                                                              ggAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCtt          >  1:3137166/1‑140 (MQ=255)

                                                                                                                                  tCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTc      >  1:5285402/1‑140 (MQ=255)

CAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGC  >  NZ_CP025268/2659654‑2659927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`