BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,843,404	A→T	intergenic (‑120/‑140)	ascG ← / → ascF	DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,843,404	0	A	T	99.7%	1033.9 / NA	287	intergenic (‑120/‑140)	ascG/ascF	DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (164/122); minor base C (1/0); total (165/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

TCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAA  >  NZ_CP025268/2843268‑2843538

  cAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAct                                                                                                                                   <  1:557069/140‑1 (MQ=255)

    gCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                                                                 <  1:625168/140‑1 (MQ=255)

    gCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                                                                 <  1:4543471/140‑1 (MQ=255)

        cgtcgtCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTg                                                                                                                             <  2:3692795/140‑1 (MQ=255)

         gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGc                                                                                                                            >  2:5103787/1‑140 (MQ=255)

         gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGc                                                                                                                            >  1:605277/1‑140 (MQ=255)

         gtcgtcATCATTTTCATCCCTGTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTATCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAGTTCACTCTCTTGc                                                                                                                            <  1:4166416/140‑1 (MQ=255)

             tcatcaTTTTCATCTCTTTACACGCAATTAACGCAGTGTACAGCACCGTTTGCTGATTGTCCTTGCACAATCGGCGGGAAAAAGATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCTCTCTCTGGCTTAt                                                                                                                        <  2:2972700/140‑1 (MQ=255)

             tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAt                                                                                                                        <  2:3544927/140‑1 (MQ=255)

              catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAtt                                                                                                                       <  1:1834669/140‑1 (MQ=255)

              catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAtt                                                                                                                       <  1:203793/140‑1 (MQ=255)

               atcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAttt                                                                                                                      >  1:1241375/1‑140 (MQ=255)

               atcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAttt                                                                                                                      >  2:229883/1‑140 (MQ=255)

                     ttCATCCCTTTACCCACAATCAACGCAGTGTACTGCACCGTTTGCCGCTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGCACAATTCACTCTCTTGCTTATTTAGTGAc                                                                                                                <  1:1012928/140‑1 (MQ=255)

                     ttCATCCCTTTACACGCAATCAACGGAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGAc                                                                                                                >  2:5024196/1‑140 (MQ=255)

                     ttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAACATGAACAATTCACTCTCTTGCTTATTTAGTGAc                                                                                                                <  2:3941/140‑1 (MQ=255)

                       cATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                                                                              <  1:2587319/140‑1 (MQ=255)

                       cATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                                                                              <  1:2978886/140‑1 (MQ=255)

                         tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACt                                                                                                            >  1:4326732/1‑140 (MQ=255)

                                  acGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAt                                                                                                   >  1:257125/1‑140 (MQ=255)

                                      aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtg                                                                                               >  2:2034322/1‑140 (MQ=255)

                                      aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtg                                                                                               >  2:1504930/1‑140 (MQ=255)

                                      aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtg                                                                                               >  1:645518/1‑140 (MQ=255)

                                            cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAc                                                                                         >  1:2126420/1‑140 (MQ=255)

                                            cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAc                                                                                         <  1:4827156/140‑1 (MQ=255)

                                             gCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                                                                        <  2:5271298/140‑1 (MQ=255)

                                               aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                      <  1:2320555/140‑1 (MQ=255)

                                                gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCggg                                                                                     >  2:5331942/1‑139 (MQ=255)

                                                gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt                                                                                     >  2:4826764/1‑140 (MQ=255)

                                                gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt                                                                                     >  2:3872068/1‑140 (MQ=255)

                                                gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTGTTGAAACCGGt                                                                                     >  1:4009565/1‑140 (MQ=255)

                                                gcgtACTGCACCGTTTGCCGACTGCCCTTGCACAACCGCCGGGCAGAACATTCAGGTGGCCGGACTCCCCAATATACAAAACGAACAACTCACCCTCTCGCTGATTTACTGACCACTACTCCTGAGCTTGTGAAGCCGGt                                                                                     >  2:5575147/3‑140 (MQ=255)

                                                 tgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGtt                                                                                    >  2:3571784/1‑140 (MQ=255)

                                                  gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                                                                   >  1:3884191/1‑140 (MQ=255)

                                                  gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                                                                   >  2:1668122/1‑140 (MQ=255)

                                                     cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCtt                                                                                <  2:3313318/140‑1 (MQ=255)

                                                        cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                                                             >  2:2890380/1‑140 (MQ=255)

                                                        cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTATCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACAGGTTTCTTAAt                                                                             >  1:5244000/1‑140 (MQ=255)

                                                         aCCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTCTTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAtt                                                                            <  1:4269187/140‑1 (MQ=255)

                                                           cGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTcc                                                                          >  2:2243620/1‑140 (MQ=255)

                                                           cGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTcc                                                                          >  1:4702388/1‑140 (MQ=255)

                                                           cGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTcc                                                                          >  1:5303052/1‑140 (MQ=255)

                                                           cGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAGTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTcc                                                                          >  2:1542324/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACANTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:2449764/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAGTATTCAGGTGAGCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGCAACCGGTTTCTTGATTCCGt                                                                        >  2:256724/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGAc                                                                                                                <  1:2285829/100‑1 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  2:925194/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:4839183/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:1357687/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:3788573/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:3185998/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  2:3267871/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:2779921/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACGATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:3133273/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTGCACAAATATAAAAAATGAAAAATTCACTCTCTTGCTTATTTAGTGACAACTATTCCTGATTTTGTGAAACCGGTTGCTTAATTCCGt                                                                        >  2:5198662/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTGCCACACATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACGACTATTCATGATTGTGTGAAACCGGTTTCTTAAttgggt                                                                        >  2:4745954/1‑136 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                                                                        >  1:4442860/1‑140 (MQ=255)

                                                             tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTGTCTTAATTCCGt                                                                        >  2:4507452/1‑140 (MQ=255)

                                                               tGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTGTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATGTTGTGAAACGGGTTTCTTAATTTCGttt                                                                      >  1:3800593/1‑140 (MQ=255)

                                                                  cGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTACTTAGTAACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAg                                                                   <  2:3114384/140‑1 (MQ=255)

                                                                  cGATTGTCCTTGCACAAACGGCGGGAAAAATATCCAGGTGACCGACACCACAAATATAAAAAATGAACCATTCACTCTCTTGCTCATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAg                                                                   >  2:362426/1‑140 (MQ=255)

                                                                     ttGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCAt                                                                <  2:5443711/140‑1 (MQ=255)

                                                                                  aTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg                                                   >  1:665074/1‑140 (MQ=255)

                                                                                  aTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg                                                   >  2:5333288/1‑140 (MQ=255)

                                                                                  aTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg                                                   >  1:5469991/1‑140 (MQ=255)

                                                                                   tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGt                                                  >  1:2395509/1‑140 (MQ=255)

                                                                                    cggcggGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTc                                                 >  1:2670334/1‑140 (MQ=255)

                                                                                           acaaaTATTCCCGTGACCGATTTCACAAATATAAAAAATGAACAATTCCCTCTCTTGCTTATTTAGTGACAACTATTAATGATTTTGTGAAACGGGTTTCTTATTTCCGTTTCAGCGTCGGCCTTTTTCCGTCACGTCGa                                          <  2:552952/138‑1 (MQ=255)

                                                                                           aaaaaTATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATATAGTGACACCTATTCATGATTTTGTGAAACGGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGa                                          <  2:4932017/140‑1 (MQ=255)

                                                                                                tatTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGAt                                     <  1:5204566/140‑1 (MQ=255)

                                                                                                tatTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGAt                                     <  1:1445215/140‑1 (MQ=255)

                                                                                                     aGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaacaa                                >  1:433774/1‑140 (MQ=255)

                                                                                                      gatGACCGGTTTCACAAATATAAAAAATGAACAATTCTCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAATCCGGTTTCTTAATTCCCTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaacaac                               <  2:1228762/138‑1 (MQ=255)

                                                                                                       gTGACCGATTTCACAAATATAAAAAATGAACACTTCACTCTCTTGCTTATTTAGTGACAACTAGTCATGATTTTGTGAAACCGGTTTCGCAATTCCGTTGCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACACCt                              <  2:2210022/140‑1 (MQ=255)

                                                                                                         gACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTAc                            <  2:702739/140‑1 (MQ=255)

                                                                                                         gACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTAc                            <  2:3384089/140‑1 (MQ=255)

                                                                                                         gACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTAc                            <  1:5103787/140‑1 (MQ=255)

                                                                                                         gACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTAc                            <  1:2495289/140‑1 (MQ=255)

                                                                                                          aCCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACa                           >  1:4617578/1‑140 (MQ=255)

                                                                                                           ccGGTTTCACACATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAATCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACAt                          <  2:2636214/140‑1 (MQ=255)

                                                                                                           ccGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACAt                          >  1:1075768/1‑140 (MQ=255)

                                                                                                              gTTTCACAAATATAAAAAATGATCAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAACCCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCtc                       <  2:273776/140‑2 (MQ=255)

                                                                                                                ttCACAAATATAAAAAATGAACACTTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc                     <  2:410056/140‑1 (MQ=255)

                                                                                                                ttCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTCATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc                     <  2:101111/140‑1 (MQ=255)

                                                                                                                ttCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc                     <  2:4450676/140‑1 (MQ=255)

                                                                                                                ttCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc                     <  1:2856091/140‑1 (MQ=255)

                                                                                                                ttCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc                     <  1:3808577/140‑1 (MQ=255)

                                                                                                                        tataAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGAt             <  2:3787382/140‑1 (MQ=255)

                                                                                                                         ataAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTGAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATa            >  2:4203455/1‑140 (MQ=255)

                                                                                                                          taAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATaa           <  1:1668122/140‑1 (MQ=255)

                                                                                                                           aaaaaaTGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAAc          <  1:937688/140‑1 (MQ=255)

                                                                                                                           aaaaaaTGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAAc          <  1:925194/140‑1 (MQ=255)

                                                                                                                           aaaaaaTGAACAATGCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTGGCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAAc          <  2:2809709/140‑1 (MQ=255)

                                                                                                                            aaaaaTGAACACTTGACTCTCTTGCTTATTTAGTGTCAACTATTCGTGATTTTGTGAACCCGGTTTCTTTTTTCCGTTTTAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACTTCTACCCTACTGATATCa         <  2:1106541/140‑1 (MQ=255)

                                                                                                                             aaaaTGAACAATTCACTCTCGTGCTTATTTAGTGACAACTATGCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTGCGTCACGTCGACTGATAACAACTACATCTACCCTACAGATAACAg        >  1:5342059/1‑140 (MQ=255)

                                                                                                                               aaTGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGa      <  2:599346/140‑1 (MQ=255)

                                                                                                                                   aaCAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATaaa  >  1:750750/1‑140 (MQ=255)

                                                                                                                                   aaCAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATaaa  >  2:2682885/1‑140 (MQ=255)

                                                                                                                                   aaCAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATaaa  >  1:2162810/1‑140 (MQ=255)

                                                                                                                                   aaCAATTCACTCTCTTGCTTATGTAGTTACAACGAGTCATGATTTTGTGAAACCGGTTTCTTAATTCCGGTTCAGCATCGGCATTTTTCTGTCACGTCGACTGATAACTACTACATCTGCGCTACTCATAACAGACTaaa  >  2:1758631/1‑140 (MQ=255)

TCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAA  >  NZ_CP025268/2843268‑2843538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`