BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,722	T→C	745 (TAA→TAG)	relA ←	GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,722	0	T	C	99.8%	1381.0 / NA	413	745 (TAA→TAG)	relA	GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (346/66); minor base G (1/0); total (347/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 413 total aligned reads displayed.

CGTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTAC  >  NZ_CP025268/2913585‑2913858

   aGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACt                                                                                                                                     <  2:2034773/140‑1 (MQ=255)

                    ccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGAcgcg                                                                                                                    >  2:1946961/1‑140 (MQ=255)

                                   aaCGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCg                                                                                                     <  1:2293097/140‑1 (MQ=255)

                                          tACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTg                                                                                              >  1:5245245/1‑140 (MQ=255)

                                          tACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTg                                                                                              >  1:851480/1‑140 (MQ=255)

                                            cGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                            <  2:4021596/140‑1 (MQ=255)

                                            cGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                            <  2:3833515/140‑1 (MQ=255)

                                            cGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGCCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                            <  2:4063391/140‑1 (MQ=255)

                                                aCTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAg                                                                                        >  1:2181579/1‑140 (MQ=255)

                                                  tgtgGAGCATAACCCTTTCCTCAAACCGCTATCATATGTAGGTACACTATATATCAATCTACATTGTAGATCCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGtt                                                                                      >  2:1675676/1‑140 (MQ=255)

                                                    tgGATCATAACCCTGTCCTCAAACCGCTATCATATGTAGATCCAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACGCCCGTGCAACCGACGCGCGTCGATAACAGCCGGCACCTGGTTGAGATTa                                                                                    >  1:4528386/1‑140 (MQ=255)

                                                        tCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCa                                                                                <  1:1684687/140‑1 (MQ=255)

                                                         cATAACCCTTTCCCCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGCTACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCCCGTCGATAACATCCGGCACCTGGTTGACTTTACCCAg                                                                               >  2:2786613/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCGCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:5001819/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  2:2530967/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:5336919/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  2:498023/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:1143575/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:1267874/1‑140 (MQ=255)

                                                           tAACCATTTCCTCAAACAGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATAAGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCTGCa                                                                             >  2:225359/1‑140 (MQ=255)

                                                             aCCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCAcg                                                                           <  2:2307741/140‑1 (MQ=255)

                                                              cccTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCAcgc                                                                          >  1:2984111/1‑140 (MQ=255)

                                                                 tttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcc                                                                       >  1:727746/1‑140 (MQ=255)

                                                                   tCCTCAACCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCCACCGACGCCCGTCGATAACATCCGGCACCCGGTTGAGTTTACCCAGCACGCGcccc                                                                     <  2:5223159/140‑1 (MQ=255)

                                                                   tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACTAGAAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                                     >  2:1162361/1‑140 (MQ=255)

                                                                   tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                                     >  1:4919333/1‑140 (MQ=255)

                                                                     cTCAACCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                                   <  2:3059231/140‑1 (MQ=255)

                                                                     cTCAAACCGCTTTCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                                   <  2:2931277/140‑1 (MQ=255)

                                                                              cTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAg                                                          >  1:5494475/1‑140 (MQ=255)

                                                                               tATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCCGCACTTGCAgg                                                         >  1:4053479/1‑140 (MQ=255)

                                                                                    tatGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTa                                                    >  2:360365/1‑140 (MQ=255)

                                                                                    tatGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTa                                                    <  2:2181594/140‑1 (MQ=255)

                                                                                     atGTAGATACAGTCTATATCAATCTAAATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTaa                                                   <  2:2822415/140‑1 (MQ=255)

                                                                                      tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTaaa                                                  >  1:1001626/1‑140 (MQ=255)

                                                                                      tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTaaa                                                  >  1:3160467/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  2:4186977/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  1:5122573/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  1:2710223/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  1:4100439/1‑140 (MQ=255)

                                                                                             aCAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAAt                                           >  1:4598596/1‑140 (MQ=255)

                                                                                               aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATgg                                         >  2:2750524/1‑140 (MQ=255)

                                                                                               aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATgg                                         >  1:5370480/1‑140 (MQ=255)

                                                                                                gTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGt                                        >  2:4490223/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:5318854/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAACCTCAATGGTc                                       >  2:1833507/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATGGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:2907397/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                  >  2:4756143/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                  >  2:3144663/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGGTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                  >  2:1065905/1‑140 (MQ=255)

                                                                                                         aaTCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGAt                               >  1:1452570/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGCGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGACGATACCATCCGGCACCTGGTTGCGTTTACCCAGCACGCGCCGCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:4391467/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTGCCGGGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGGTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCAGGTCGATgg                             >  1:4768790/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:4623297/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:332894/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:3417202/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:3507723/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:1849169/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:178824/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:1990060/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:75761/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:3659144/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:3799316/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:1317419/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:641575/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:685560/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:5547072/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:765164/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:2603514/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:638500/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:2204850/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:2511248/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:1778502/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGCTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:2693057/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGCTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGAtcg                             >  2:4676205/1‑138 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCGAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:4868459/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGAAAATTTCGGCCTAAATACCGTGCAACCGAAGCTCGTAGATAACATAAGGAACCTGGTTGAGTTTACCCAGCAAGTGCCCTAGCACGTACAGGTTGTAAATCACAATGGTCATGTAGAggg                             >  2:492407/1‑140 (MQ=255)

                                                                                                           gcTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:336623/2‑140 (MQ=255)

                                                                                                             tACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTc                           >  2:1987833/1‑140 (MQ=255)

                                                                                                               cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                         >  2:1617353/1‑140 (MQ=255)

                                                                                                               cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                         >  2:5563834/1‑140 (MQ=255)

                                                                                                               cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                         >  2:5398955/1‑140 (MQ=255)

                                                                                                                aTTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGcc                        <  1:3578132/140‑1 (MQ=255)

                                                                                                                  tGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAg                      <  1:606685/140‑1 (MQ=255)

                                                                                                                     aGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTg                   >  1:3279909/1‑140 (MQ=255)

                                                                                                                     aGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTg                   <  1:4224776/140‑1 (MQ=255)

                                                                                                                       aTACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCt                 >  2:897191/1‑140 (MQ=255)

                                                                                                                         aCGAGCAAATTTCGGCCTAACTCCCTTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGCTGGTCGCCAGTTGCTGt               >  2:3438015/1‑140 (MQ=255)

                                                                                                                         aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt               >  1:2370817/1‑140 (MQ=255)

                                                                                                                           gAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGttt             <  1:965838/140‑1 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:3805991/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:43315/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:3266720/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:266297/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:2444534/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  1:905708/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:531474/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  1:401072/1‑140 (MQ=255)

                                                                                                                              cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          >  1:2808318/1‑140 (MQ=255)

                                                                                                                              cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          >  1:3692416/1‑140 (MQ=255)

                                                                                                                                      ggCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGATCGCCAGTTGCTGTTTGGTGTCGCTAc  >  1:4449157/1‑140 (MQ=255)

CGTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTAC  >  NZ_CP025268/2913585‑2913858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`