BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,084,342	C→T	D42D (GAC→GAT)	loiP →	metalloprotease LoiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,084,342	0	C	T	99.7%	1082.4 / NA	290	D42D (GAC→GAT)	loiP	metalloprotease LoiP
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (170/119); minor base G (0/1); total (170/120)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

GAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGACGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGC  >  NZ_CP025268/3084206‑3084478

gAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:4080921/140‑1 (MQ=255)

gAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:3620791/140‑1 (MQ=255)

gAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:5449110/140‑1 (MQ=255)

gAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:2482986/140‑1 (MQ=255)

gAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:294013/140‑1 (MQ=255)

gAAGAGAAAACATGAAAATTCGCGCATTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:3763798/140‑1 (MQ=255)

gAAGAGAAAACATGAAAATTCGCGACTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                       <  1:2355827/140‑1 (MQ=255)

  agagAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCa                                                                                                                                     <  2:412229/140‑1 (MQ=255)

    agaAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGCAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAgg                                                                                                                                   >  2:1597478/1‑140 (MQ=255)

      aaaaCATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTg                                                                                                                                 >  2:2546902/1‑140 (MQ=255)

           aTGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAAc                                                                                                                            <  1:1893073/140‑1 (MQ=255)

                     gcgcCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGAt                                                                                                                  <  2:4295482/140‑1 (MQ=255)

                     gcgcCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGAt                                                                                                                  >  2:4008398/1‑140 (MQ=255)

                         cTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAgg                                                                                                              >  2:190712/1‑140 (MQ=255)

                         cTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAgg                                                                                                              <  1:4900432/140‑1 (MQ=255)

                         cTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAgg                                                                                                              <  2:1773519/140‑1 (MQ=255)

                                   gCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGga                                                                                                    >  1:873808/1‑140 (MQ=255)

                                   gCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGga                                                                                                    >  1:2180113/1‑140 (MQ=255)

                                      aTGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGAt                                                                                                 >  2:4460362/1‑140 (MQ=255)

                                       tGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                                                                                <  1:4270834/140‑1 (MQ=255)

                                         aGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGa                                                                                              >  1:5264752/1‑140 (MQ=255)

                                         aGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGa                                                                                              <  2:2705458/140‑1 (MQ=255)

                                             tGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGc                                                                                          <  1:1219599/140‑1 (MQ=255)

                                              ggCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCCGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCa                                                                                         >  2:1742091/1‑140 (MQ=255)

                                                  aCGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGc                                                                                     <  2:2063675/140‑1 (MQ=255)

                                                  aCGGTACTGACAGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGCCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGc                                                                                     <  1:3137662/140‑1 (MQ=255)

                                                   cGGTACTGACTGGTTGCCAGAATATGGGCTCCAACGGACTGCTCTCCTCAGGCGCGGAGGCTTTTCGGGCTTATATTTTGAGTGATGCGCAGGGGTAAACCCTGCGCGATCATGAGTGTCAGGATATGGACAGCAAgggg                                                                                    >  2:1815011/1‑138 (MQ=255)

                                                     gTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcgac                                                                                  >  1:2239109/1‑140 (MQ=255)

                                                        ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTGTCATCAGGAGCGGAAGCGTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                                                                               >  2:900159/1‑140 (MQ=255)

                                                        ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                                                                               <  1:1390320/140‑1 (MQ=255)

                                                        ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                                                                               >  2:1362446/1‑140 (MQ=255)

                                                           actgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                                                                            >  2:5372783/1‑140 (MQ=255)

                                                           actgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                                                                            >  1:4842764/1‑140 (MQ=255)

                                                           actgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                                                                            >  2:1286175/1‑140 (MQ=255)

                                                            ctgGTTGCCAGAATATGGCCTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTCCAGGCTTACAGTTTGCGAGATGCGCAGGTGAAACCCCTGCGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                                                                           <  2:723572/140‑1 (MQ=255)

                                                            ctgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                                                                           <  2:4754029/140‑1 (MQ=255)

                                                            ctgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                                                                           <  1:3638649/140‑1 (MQ=255)

                                                            ctgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                                                                           <  1:5093539/140‑1 (MQ=255)

                                                            ctgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                                                                           <  1:5547051/140‑1 (MQ=255)

                                                            ctgGTTGCCAGAATATGGACTCCAACGCACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAACACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                                                                           <  1:4390295/140‑1 (MQ=255)

                                                             tgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcgc                                                                          >  2:2117750/1‑140 (MQ=255)

                                                               gTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgcca                                                                        >  1:1590224/1‑140 (MQ=255)

                                                               gTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgcca                                                                        <  2:3467271/140‑1 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  1:4016425/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTG‑AAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagc                                                                      >  1:3889867/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGCTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  2:3933227/1‑140 (MQ=255)

                                                                 tGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagc                                                                      <  1:3527369/140‑1 (MQ=255)

                                                                  gCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcc                                                                     >  1:3151406/1‑140 (MQ=255)

                                                                       aaTATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAg                                                                >  2:1544054/1‑140 (MQ=255)

                                                                         tatGGACTCCAACGGACTGCTCTCATCAGGCGCGGAAGCTTTTCAGGCTAACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCg                                                              >  2:3807537/1‑140 (MQ=255)

                                                                          atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGa                                                             <  1:2457492/140‑1 (MQ=255)

                                                                          atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGa                                                             >  1:804272/1‑140 (MQ=255)

                                                                          atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGa                                                             >  2:247950/1‑140 (MQ=255)

                                                                           tGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGaa                                                            <  2:5407574/140‑1 (MQ=255)

                                                                           tGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGaa                                                            <  1:214024/140‑1 (MQ=255)

                                                                            ggACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAAt                                                           >  1:389988/1‑140 (MQ=255)

                                                                              aCTCCAACGGACTGCTCTCATCAGGAGCGGAAGGTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGGGAAAAGCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCGGCGAATAGGGAATAc                                                         >  2:5246006/1‑140 (MQ=255)

                                                                                 ccAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCt                                                      >  2:550649/1‑140 (MQ=255)

                                                                                  cAACGTACTGCTCTAATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGCGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTa                                                     <  2:3157234/140‑1 (MQ=255)

                                                                                  cAACGGACTGCTCCCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATAGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTa                                                     <  2:5118480/140‑1 (MQ=255)

                                                                                    aCGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTaaa                                                   <  2:2405972/140‑1 (MQ=255)

                                                                                    aCGGACTGCTCTCAGCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTaaa                                                   <  1:3821069/140‑1 (MQ=255)

                                                                                     cGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAAc                                                  >  2:562235/1‑140 (MQ=255)

                                                                                        aCTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTc                                               >  1:4580243/1‑140 (MQ=255)

                                                                                          tGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTg                                             >  1:994969/1‑140 (MQ=255)

                                                                                           tctatcatcaAGAGCGGAAGCTTTTCAGGCTTACAGTTAGAGTGATGCGCGTGTGAAAACCCTGACGGATCAGGCATGGCAGGAGATGGACAGCAAGGCGAAGATTGCCCCAGCCAATAGCGAATACGCTAAACGTCTGa                                            <  2:1028635/136‑1 (MQ=255)

                                                                                           gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGa                                            <  1:4751648/140‑1 (MQ=255)

                                                                                           gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTAAGTGATTCGCAGGTGAAACCCCTGAGCGATCAGGCATGTCAGGAGATGGACACAAAGGCGCCGATTGCGCGAGCCAATAGCGAATACGCTAAACGTTTGa                                            <  2:1194471/140‑1 (MQ=255)

                                                                                           gCTCGCATCAGGAGCGGAAGCGGTTCAGGCTTACAGTTTGAGTGAGGCGCAGGTGAAAACCCTGAGCGATCAGGCGTGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGa                                            <  2:2317351/140‑1 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGAc                                           >  2:2389625/1‑140 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGAc                                           >  1:2270367/1‑140 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGAc                                           >  2:2988731/1‑140 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTGTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCGAAACGTCTGAc                                           >  1:2794341/1‑140 (MQ=255)

                                                                                                   caGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTAtt                                    <  1:3741941/140‑1 (MQ=255)

                                                                                                      gAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGcc                                 >  1:4421439/1‑140 (MQ=255)

                                                                                                      gAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGcc                                 >  1:4212350/1‑140 (MQ=255)

                                                                                                      gAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGcc                                 >  2:168301/1‑140 (MQ=255)

                                                                                                      gAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGGGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGCCGATTGCGCCAGCCCATCGCGAATACGCTAAACGTCTGACAACTATTGcc                                 >  2:1574406/1‑140 (MQ=255)

                                                                                                       aGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGTGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCa                                <  1:607912/140‑1 (MQ=255)

                                                                                                       aGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCa                                <  2:5083186/140‑1 (MQ=255)

                                                                                                       aGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCa                                <  1:2574894/140‑1 (MQ=255)

                                                                                                        gCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCaa                               >  2:3568504/1‑140 (MQ=255)

                                                                                                         cGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAAt                              >  2:4897093/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGCGATGGACAGCAAGGCGACGGTGGGGGCAGCGAATAGCGAATACGCGAAACGTCGGAGAACTATTGCCAATgc                            >  2:2594172/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgc                            >  2:2795593/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgc                            >  1:3469493/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgc                            >  1:3250819/1‑140 (MQ=255)

                                                                                                            aaGCTTTTCAGGCTTACAGTTTGCGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgcg                           <  1:1428508/140‑1 (MQ=255)

                                                                                                            aaGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACAATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgcg                           <  2:2180955/140‑1 (MQ=255)

                                                                                                                ttttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAg                       >  1:3003144/1‑140 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCCGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  1:2660983/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  1:1941590/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  2:4632099/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  1:4955743/140‑1 (MQ=255)

                                                                                                                   tCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGca                    <  2:3437384/140‑1 (MQ=255)

                                                                                                                      ggCCTACAGTTGGAGTGATGCGAAGGTGAAAGCCATGAGCGATAAAGCATGTCAGGAGATGGACAGCAAGGCGACGATTGAGCCAGCCAATAGAGAATACGCTAAACGTCGGACAACTATTGCCAATGCGCTAGGcaaca                 <  2:4990446/140‑1 (MQ=255)

                                                                                                                          tACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAatat             >  2:2943071/1‑140 (MQ=255)

                                                                                                                                 tGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGt      <  1:5155635/140‑1 (MQ=255)

                                                                                                                                     tGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGc  <  1:1722076/140‑1 (MQ=255)

                                                                                                                                     tGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGc  <  1:2723144/140‑1 (MQ=255)

GAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGACGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGC  >  NZ_CP025268/3084206‑3084478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`