BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,137,127	A→G	N231S (AAT→AGT)	yghT →	dTMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,137,127	0	A	G	99.7%	1173.1 / NA	353	N231S (AAT→AGT)	yghT	dTMP kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (178/174); minor base C (0/1); total (178/175)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

TGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATG  >  NZ_CP025268/3136996‑3137260

  ccATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATg                                                                                                                              >  1:3284326/1‑140 (MQ=255)

   cATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATgg                                                                                                                             <  1:2492821/140‑1 (MQ=255)

    aTAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGa                                                                                                                            <  1:5125094/140‑1 (MQ=255)

     tAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGaa                                                                                                                           >  1:2841863/1‑140 (MQ=255)

       aCGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAt                                                                                                                         >  1:1675030/1‑140 (MQ=255)

       aCGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAt                                                                                                                         >  2:4586313/1‑140 (MQ=255)

        cGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAACAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATg                                                                                                                        >  2:2093397/1‑140 (MQ=255)

          ccACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGGGTTGGTTGATGCAGAAAAAGCGATTAAGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGcc                                                                                                                      >  1:1384123/1‑140 (MQ=255)

            ccaGTAAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                    <  2:319492/139‑1 (MQ=255)

            acaGTTAACCTTTGGTGGTGCATCACTGGTTGATATCGATGCCAATAAGCCACTGGCACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTCGTTATCACTAGTCAAAAATGGAAATACCCg                                                                                                                    <  2:4339236/140‑1 (MQ=255)

            acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGCACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCGCTAGTCAAAAATGGAAACGCCCg                                                                                                                    <  2:1200215/140‑1 (MQ=255)

            acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                    <  2:90955/140‑1 (MQ=255)

            acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                    <  2:1328532/140‑1 (MQ=255)

            acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                    <  2:3284326/140‑1 (MQ=255)

            acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                    <  1:2093397/140‑1 (MQ=255)

             caGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGa                                                                                                                   >  1:1738653/1‑140 (MQ=255)

             caGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGa                                                                                                                   >  1:1214708/1‑140 (MQ=255)

                  aaCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGcc                                                                                                              >  1:2537914/1‑140 (MQ=255)

                  aaCCTTTGGCGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGcc                                                                                                              >  2:636956/1‑140 (MQ=255)

                        tggtggTGCACACCTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  2:5531953/1‑140 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAt                                                                                                                         >  1:5471590/1‑123 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  2:4255656/1‑140 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  2:5367566/1‑140 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  1:863654/1‑140 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  1:1068404/1‑140 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  1:3232908/1‑140 (MQ=255)

                        tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                        >  1:1891861/1‑140 (MQ=255)

                          gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                                      >  1:2225165/1‑140 (MQ=255)

                              tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAt                                                                                                  >  2:1260951/1‑140 (MQ=255)

                              tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAt                                                                                                  >  2:5229162/1‑140 (MQ=255)

                               gCACAACTGGTTGATATCGCTGCCAATCAGCCACTGGAACCAGTGTTGGTTGCTGCAGAAAAAGCGCTTACGGATTTTATGACCGCGC‑GTGGTTATCACTCGCAAGCAATGGAAATGCCCGATCGCCAGGACCggcccac                                                                                                 >  2:3930695/1‑135 (MQ=255)

                               gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                 >  2:2355077/1‑140 (MQ=255)

                                cacaACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAttt                                                                                                >  1:34365/1‑140 (MQ=255)

                                cacaACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGCGTTGGTTGATGCAGAAAAAGCGATGACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAttt                                                                                                >  1:4220598/1‑140 (MQ=255)

                                     cTGGTTGATATCGATGCCAATCAGCCACCGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                           <  2:4650240/140‑1 (MQ=255)

                                        gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                        >  2:3591720/1‑140 (MQ=255)

                                        gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                        >  2:1957560/1‑140 (MQ=255)

                                          tGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGt                                                                                      <  2:1399284/140‑1 (MQ=255)

                                           gATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTTATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGtt                                                                                     <  1:1408343/140‑1 (MQ=255)

                                             tatCGATGCCAATCAGCCACTGGAACAGGTGTCGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCAGTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                                   >  1:4106637/1‑140 (MQ=255)

                                                cGATTCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                >  2:4126018/1‑140 (MQ=255)

                                                cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                <  2:4988581/140‑1 (MQ=255)

                                                  aTGCCAATCATCCACTGGAACAGGTGTTGTTTGATGCAGAAAAAGCGATTACGGATTTTAAGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCACGACCTGGCATGTTCACGAAGGCTAAATCaa                                                                              >  1:2800549/1‑140 (MQ=255)

                                                  aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGa                                                                                          <  2:2723810/128‑1 (MQ=255)

                                                  aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                              >  2:4076890/1‑140 (MQ=255)

                                                  aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                              <  1:3897650/140‑1 (MQ=255)

                                                  aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                              <  2:4760277/140‑1 (MQ=255)

                                                     ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAATTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                           <  2:156112/140‑1 (MQ=255)

                                                     ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                           <  1:1844390/140‑1 (MQ=255)

                                                     ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                           <  1:4476963/140‑1 (MQ=255)

                                                     ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                           <  2:4709257/140‑1 (MQ=255)

                                                        aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCaa                                                                        >  1:736080/1‑140 (MQ=255)

                                                         tCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAAt                                                                       >  1:532828/1‑140 (MQ=255)

                                                          cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGTTTAAATCAACTGCAATg                                                                      <  2:2792118/140‑1 (MQ=255)

                                                          cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                      <  2:3231844/140‑1 (MQ=255)

                                                          cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                      <  2:3644972/140‑1 (MQ=255)

                                                          cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                      <  1:2963334/140‑1 (MQ=255)

                                                          cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                      <  1:310521/140‑1 (MQ=255)

                                                          cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                      <  2:1872378/140‑1 (MQ=255)

                                                             ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                   <  2:147029/140‑1 (MQ=255)

                                                             ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                   <  2:2139937/140‑1 (MQ=255)

                                                             ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCCATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                   >  2:4478691/1‑140 (MQ=255)

                                                             ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑CTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                   <  1:4666074/140‑1 (MQ=255)

                                                                    aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                            <  1:2729882/140‑1 (MQ=255)

                                                                        ggTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                           <  1:2891619/121‑1 (MQ=255)

                                                                        ggTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                           >  2:2891619/1‑121 (MQ=255)

                                                                             tggttggtGCAGAAAAAGCGATTACGGATTTGATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGTCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                   <  2:3471018/140‑1 (MQ=255)

                                                                             tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                   >  1:3168962/1‑140 (MQ=255)

                                                                              ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                    <  1:4265660/74‑1 (MQ=255)

                                                                              ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacca                                                  >  2:71885/1‑140 (MQ=255)

                                                                              ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATAGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACGGCAATGCTATCCAGTACAGCccaccc                                                  >  2:3000485/1‑139 (MQ=255)

                                                                               gttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCag                                                 >  1:5235575/1‑140 (MQ=255)

                                                                                   aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTGGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                             >  2:5260016/1‑140 (MQ=255)

                                                                                   aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                             >  1:1023079/1‑140 (MQ=255)

                                                                                   aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                             >  1:3665598/1‑140 (MQ=255)

                                                                                   aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAAGCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                             >  1:5527760/1‑140 (MQ=255)

                                                                                    tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTCGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaaga                                            >  2:180076/1‑140 (MQ=255)

                                                                                    tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGTATTGTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaaga                                            >  1:3993803/1‑140 (MQ=255)

                                                                                    tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaaga                                            >  1:4345635/1‑140 (MQ=255)

                                                                                    tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaaga                                            >  1:3411867/1‑140 (MQ=255)

                                                                                         aaaaaGCGATTACGGATTTTATGACCGCGCGG‑GGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAAt                                       >  2:573106/1‑140 (MQ=255)

                                                                                             aGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc                                   <  1:4810298/140‑1 (MQ=255)

                                                                                              gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                                  <  2:4793622/140‑1 (MQ=255)

                                                                                              gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTaccg                                  >  1:5400964/1‑140 (MQ=255)

                                                                                               cGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgc                                 <  2:3855541/140‑1 (MQ=255)

                                                                                                    aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                            <  1:3323321/140‑1 (MQ=255)

                                                                                                    aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                            <  2:3670885/140‑1 (MQ=255)

                                                                                                     cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                           <  2:5139846/140‑1 (MQ=255)

                                                                                                               gACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc                 <  1:647279/140‑1 (MQ=255)

                                                                                                               gACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc                 <  1:2341138/140‑1 (MQ=255)

                                                                                                                 ccGCGC‑GTGGTTATCACTAGTCCAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa               <  1:2661852/140‑1 (MQ=255)

                                                                                                                 ccGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGAAAAGTAAATACCCa               >  1:4898213/1‑140 (MQ=255)

                                                                                                                  cgcgc‑gtGGTTATCACTAGTCAACAATGGAAATGCCCGATCGCCAGGCCCGGGCATTTGCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGCAAGAAAAATTGCCGCCGCTAAAGTAAATACCCAc              >  2:1085589/1‑140 (MQ=255)

                                                                                                                     cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc            >  1:3819884/6‑140 (MQ=255)

                                                                                                                     cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc            >  1:2126042/6‑140 (MQ=255)

                                                                                                                     cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc            >  2:3696965/6‑140 (MQ=255)

                                                                                                                     cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc            >  1:1172212/6‑140 (MQ=255)

                                                                                                                        gtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAt         <  2:3065506/138‑1 (MQ=255)

                                                                                                                        gtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAt         <  2:1664974/138‑1 (MQ=255)

                                                                                                                            ttATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAGAGTAAATACCCACGCCATCAgg     <  1:3882897/140‑1 (MQ=255)

TGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATG  >  NZ_CP025268/3136996‑3137260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`