BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,183,692	A→G	S153G (AGT→GGT)	zupT →	zinc transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,183,692	0	A	G	99.2%	877.2 / NA	267	S153G (AGT→GGT)	zupT	zinc transporter ZupT
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (144/121); minor base T (1/1); total (145/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCC  >  NZ_CP025268/3183555‑3183826

    aGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCGGGAGCTGGGACTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtgg                                                                                                                                  >  1:1558179/1‑140 (MQ=255)

         ttCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtg                                                                                                                             >  1:590325/1‑140 (MQ=255)

          tCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                            <  2:166831/140‑1 (MQ=255)

          tCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                            >  1:2834163/1‑140 (MQ=255)

           cTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtggc                                                                                                                           >  1:3485062/1‑140 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGTGATTTTGCATCGCACTGGCGGTCTCGTTGCACAATATCCCTGAATGTCTGGCAGTGGCAgg                                                                                                                        >  2:3172845/1‑140 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATCTCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:285062/1‑140 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:5540543/1‑140 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:1898234/1‑140 (MQ=255)

              cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:1259793/1‑140 (MQ=255)

                cACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGcc                                                                                                                      >  1:1655754/1‑140 (MQ=255)

                   tctcGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCgg                                                                                                                   >  1:3319803/1‑140 (MQ=255)

                    ctcGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGt                                                                                                                  <  1:4410836/140‑1 (MQ=255)

                      cGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGttt                                                                                                                >  1:786356/1‑140 (MQ=255)

                        gCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTAt                                                                                                              >  1:733793/1‑140 (MQ=255)

                           tCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCg                                                                                                           <  2:3465741/140‑1 (MQ=255)

                             aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGc                                                                                                         >  1:2536546/1‑140 (MQ=255)

                             aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGc                                                                                                         >  1:5344166/1‑140 (MQ=255)

                               tCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                       >  1:3155714/1‑140 (MQ=255)

                                cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                      <  1:2539360/140‑1 (MQ=255)

                                 tGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                                     <  2:72424/140‑1 (MQ=255)

                                  gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                                    <  1:4440873/140‑1 (MQ=255)

                                  gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                                    <  1:4506092/140‑1 (MQ=255)

                                   cATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACggg                                                                                                   >  2:241478/1‑140 (MQ=255)

                                   cATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACggg                                                                                                   >  2:3299491/1‑140 (MQ=255)

                                         ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaa                                                                                             >  2:1115189/1‑140 (MQ=255)

                                         ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaa                                                                                             >  1:5153906/1‑140 (MQ=255)

                                          tCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaaa                                                                                            <  1:307658/140‑1 (MQ=255)

                                           cccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAAc                                                                                           >  1:3660774/1‑140 (MQ=255)

                                           cccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAAc                                                                                           >  1:4966774/1‑140 (MQ=255)

                                           cccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAAc                                                                                           >  2:5243886/1‑140 (MQ=255)

                                            ccGGAAGGGATTGTCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTCATGCGGCAACGGGGTCTAAACg                                                                                          <  2:5405361/140‑1 (MQ=255)

                                             cGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGc                                                                                         >  2:65385/1‑140 (MQ=255)

                                              ggAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCa                                                                                        <  1:1748609/140‑1 (MQ=255)

                                                 aGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACcg                                                                                     <  1:5060672/140‑1 (MQ=255)

                                                  gggATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGCTGCACAATATCCCTGAAGGTCTGGAAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACcgc                                                                                    >  2:4063870/1‑140 (MQ=255)

                                                     aTTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGTGGCAACGGGGGCTAAACGCACCGCGAt                                                                                 >  2:1340743/1‑140 (MQ=255)

                                                     aTTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                      >  2:569045/1‑119 (MQ=255)

                                                     aTTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGAt                                                                                 >  1:5533938/1‑140 (MQ=255)

                                                      ttGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGAtt                                                                                >  1:1048945/1‑140 (MQ=255)

                                                        gCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCt                                                                              <  1:3550194/140‑1 (MQ=255)

                                                         ccACCTTTGTCACGGCGAGCAGCAAACTGGAGCTGGGATTTGGCATCGTACTGGCCGTCGCGTGGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATGCtg                                                                             <  2:2701349/140‑1 (MQ=255)

                                                              gttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCGCTGAAGGGCTGGCAGTGGCAGGCGCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATGCTGTGGGc                                                                        >  2:47802/2‑140 (MQ=255)

                                                               ttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCg                                                                       <  2:5284399/140‑1 (MQ=255)

                                                               ttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCg                                                                       <  1:2304315/140‑1 (MQ=255)

                                                               ttGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCg                                                                       >  2:4730696/1‑140 (MQ=255)

                                                                 gTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                     >  2:1607528/1‑140 (MQ=255)

                                                                       gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCTTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGGACCGCGATTCTGTGGGCGGGGGTTTc                                                               >  1:1421716/1‑140 (MQ=255)

                                                                       gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCGACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTc                                                               <  1:2373091/140‑1 (MQ=255)

                                                                       gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTc                                                               >  1:3726850/1‑140 (MQ=255)

                                                                       gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGGTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTc                                                               >  2:3857656/1‑140 (MQ=255)

                                                                       gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGAACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCTATTCTGTGGGCGGGGATTTc                                                               <  2:358451/140‑1 (MQ=255)

                                                                       gCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTGGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGGGGGGAttgc                                                               >  1:140060/1‑138 (MQ=255)

                                                                          agcagcAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCTTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGCTTCCGTGGGCGGGGATTTCTgg                                                            >  2:3985999/1‑140 (MQ=255)

                                                                          agcagcAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTgg                                                            >  1:2158085/1‑140 (MQ=255)

                                                                           gcagcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGa                                                           <  1:4116970/140‑1 (MQ=255)

                                                                           gcagcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCAAAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGa                                                           <  2:1708713/140‑1 (MQ=255)

                                                                                  ccTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAg                                                    >  2:4289971/1‑140 (MQ=255)

                                                                                  ccTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAg                                                    <  2:4990003/140‑1 (MQ=255)

                                                                                   cTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGa                                                   <  2:816137/140‑1 (MQ=255)

                                                                                      gAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAAt                                                >  1:2905762/1‑140 (MQ=255)

                                                                                         cTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                             >  1:1704968/1‑140 (MQ=255)

                                                                                         cTGGCAGTTGGCATCGCCCTGGCCGTCGCGTTGCCCAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCAACGCGATTCTGTGGGCTGGGATTCCTGGAATAGCAGAAATTCt                                             <  2:4602604/140‑1 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCGCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGTACTAGCAGAAATTCTtgg                                          >  2:1003035/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                          >  1:1095850/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                          >  1:4950909/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                          >  1:2454206/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                          >  1:5392456/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGAGTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                          >  2:2242605/1‑140 (MQ=255)

                                                                                            ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGCCAGTGGCAGGCCCGGTTTATGCGTCAACGGGGTCTAAACGCTCCGCGATTCTGTGGGCGGGGATTGCTGGACTAGCAGAAATTCGtgg                                          >  1:3366512/1‑140 (MQ=255)

                                                                                             gATTTGGCATCGCACTGGCCGTCGCGGTGCACAATATCCTTGAAGGTCTGCCAGTGGCATGCCCGGTTTATGCGGCAACGGGGTCTAAGCGGAGCGCGATTCTGTCGGCGGGGATTTCTGGTCTAGCAGAAATTCTtggt                                         <  2:5139997/140‑1 (MQ=255)

                                                                                              attgagCAGCGCACTGGCCGTCGCGTTGCACGATATCCCTGCATGTCTGGCAGTGGCATGCCGGGTTTATGCGGCAACTGGGTCTAAACGCATTGCGATTCTGTGGGCGTGGATTTCTGGACTAGCTGAAGTTCTtggtg                                        <  2:2320265/135‑1 (MQ=255)

                                                                                              aTTTGGCATCGCAGTGGCCGTCGCGTTGCACACTATCCCTGAAGGTCTGGCAGTGGCATGCCCGGTTTATGCGGCAACGCGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        <  2:2972547/140‑1 (MQ=255)

                                                                                              aTTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        <  1:2390265/140‑1 (MQ=255)

                                                                                              aTTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        <  1:4690740/140‑1 (MQ=255)

                                                                                              aTTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTATATGCGGCACCGGGGTCTAAACGAACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        <  1:1331060/140‑1 (MQ=255)

                                                                                              aTTTGGCATCGCACCGGCCGGCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGAGGCAACGGGGTCTAAACGCACGGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        <  2:4366992/140‑1 (MQ=255)

                                                                                                 tGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGgtggtg                                     >  2:1917761/1‑140 (MQ=255)

                                                                                                 tGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGgtggtg                                     <  2:3814308/140‑1 (MQ=255)

                                                                                                   gCACCGCACTGGTCGTCGCGTTGCACCATATCCCTGAAGGTCTGGTCGTGGCCGGCCCGGGTTCTGCGGCAACGGGGTATACACGCACCGCGCTTCTGCTGGCGGGGATGTCTGCACTAGCGGAAATTCTTGGTGGtgtg                                   <  1:2706088/140‑1 (MQ=255)

                                                                                                    cATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGc                                  <  1:3592470/140‑1 (MQ=255)

                                                                                                        gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                              <  1:1823212/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  2:4147279/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  1:2174899/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  1:5562898/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  2:25537/140‑1 (MQ=255)

                                                                                                             ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  1:4306902/140‑1 (MQ=255)

                                                                                                                    gcgTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt                  >  2:1441476/1‑140 (MQ=255)

                                                                                                                    gcgTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt                  <  2:1073281/140‑1 (MQ=255)

                                                                                                                    gcgGTGCACAATATCACCGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAAGTCTTGGTGGTGTGCTGGCGTGGTTAATCCt                  <  2:1969829/140‑1 (MQ=255)

                                                                                                                        tGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGt              >  1:4396670/1‑140 (MQ=255)

                                                                                                                        tGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGt              >  1:1873069/1‑140 (MQ=255)

                                                                                                                          cacaATCTCCCTGCAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCCCCGCGCTTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAg            <  2:2764823/140‑1 (MQ=255)

                                                                                                                             aaTATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTATAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGATGGCGTGGTTAATCCTCGGTAGCAt         <  1:3172845/140‑1 (MQ=255)

                                                                                                                               tatCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGa       >  1:4823156/1‑140 (MQ=255)

                                                                                                                                atCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGAt      <  1:3839191/140‑1 (MQ=255)

                                                                                                                                  cccTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGAttt    >  2:4186286/1‑140 (MQ=255)

                                                                                                                                  cccTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGAttt    >  2:670033/1‑140 (MQ=255)

                                                                                                                                    cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        <  1:2081531/102‑1 (MQ=255)

                                                                                                                                    cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtg                                        >  2:2081531/1‑102 (MQ=255)

GCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCC  >  NZ_CP025268/3183555‑3183826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`