BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,298,720	C→T	G35E (GGA→GAA)	yraQ ←	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,298,720	0	C	T	98.9%	1302.6 / NA	366	G35E (GGA→GAA)	yraQ	permease
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (178/182); minor base G (2/2); total (180/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

  gcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                                                                                                                      <  2:4123717/140‑1 (MQ=255)

   cTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAt                                                                                                                                     >  2:504206/1‑140 (MQ=255)

    tttCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAtt                                                                                                                                    <  1:3409375/140‑1 (MQ=255)

      tCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTc                                                                                                                                  <  2:1339066/140‑1 (MQ=255)

       ccATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGGCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTca                                                                                                                                 <  1:2794876/140‑1 (MQ=255)

       ccATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTca                                                                                                                                 <  1:286352/140‑1 (MQ=255)

        cATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTcac                                                                                                                                <  1:812257/140‑1 (MQ=255)

           aCCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTAGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATa                                                                                                                             >  2:4834388/1‑140 (MQ=255)

             cgcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCACGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                                                                                                                           >  2:1132792/1‑140 (MQ=255)

             cgcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                                                                                                                           >  1:1460750/1‑140 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATATAACGCCTAATGCCATGGGGTTAACATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTTGGCAGCAGTAAACGCTTTTCCGTAATAAGGTTGCCATTTCACATagt                                                                                                                           >  2:1584163/1‑138 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                                                                                                                         >  1:1160757/1‑140 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                                                                                                                         >  2:3339598/1‑140 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                                                                                                                         >  2:2013750/1‑140 (MQ=255)

               cgAGAAAGTAGATCATCGCGTAATCCAACCCCGCCTGCGAT‑GGGTTAACATCCGCCGGCGCAAGGAAAGACTGACCGATACGGGGGGTTTACGCAGCAGTACCCGCTTTACCGCGGTAAGGTTGCCATTCCACATACCag                                                                                                                         >  2:388274/1‑140 (MQ=255)

                 aGGAAATAGATCATCGCGTAATCCAACGCAGCCCGCCAT‑GGGTTAGCATCCGCCTGCGCCCGGATAGATTTACCGATACTGTGGGTTTCGGAAGCAGGAAACGCTTTGCGTTAGTAAGGTGCCGATTTCACATACAagag                                                                                                                       >  2:866102/1‑140 (MQ=255)

                  ggAAAAAGATCATCGCGTAATCCACCGCCGCCTGCCAT‑GCGTTAGCATTCGCCTGCGCAAGGATAGACTTACCCATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAgaga                                                                                                                      <  2:3419520/140‑1 (MQ=255)

                          atcatcGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAAt                                                                                                              >  1:5477268/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGTCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAAAGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGata                                                                                                          >  2:650302/1‑138 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTTTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                          >  2:4975817/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                          >  1:2662760/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                          >  1:3296459/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                          >  1:3781599/1‑140 (MQ=255)

                               cgcgTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                                         >  2:4341459/1‑140 (MQ=255)

                               cgcgTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                                         >  1:3927634/1‑140 (MQ=255)

                                 cgTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACg                                                                                                       <  1:1333501/140‑1 (MQ=255)

                                               gccTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                                         <  2:5273341/140‑1 (MQ=255)

                                               gccTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                                         <  2:4441306/140‑1 (MQ=255)

                                               gccTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTGTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                                         <  2:1696836/140‑1 (MQ=255)

                                                ccTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  1:1029967/1‑140 (MQ=255)

                                                ccTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  1:2422347/1‑140 (MQ=255)

                                                ccTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  1:3633485/1‑140 (MQ=255)

                                                 cTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                       <  2:3618434/140‑1 (MQ=255)

                                                 cTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATCTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                       <  1:4786728/140‑1 (MQ=255)

                                                    ccAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGtt                                                                                    >  1:4169954/1‑140 (MQ=255)

                                                         gggTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTcca                                                                                <  2:4623251/140‑1 (MQ=255)

                                                          ggTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccac                                                                               <  2:3896807/140‑1 (MQ=255)

                                                            ttcgCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                             <  2:3670060/137‑1 (MQ=255)

                                                                    cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCATATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                     >  1:3118006/1‑140 (MQ=255)

                                                                    cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                     >  1:3582317/1‑140 (MQ=255)

                                                                    cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                     >  1:2545515/1‑140 (MQ=255)

                                                                    cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                     >  2:107057/1‑140 (MQ=255)

                                                                     gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  1:4627446/1‑140 (MQ=255)

                                                                     gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  2:1400320/1‑140 (MQ=255)

                                                                     gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  1:837331/1‑140 (MQ=255)

                                                                     gCCTGCGCAAGGAGAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCATAGTCAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCGACACCGCATggg                                                                    >  2:5328139/1‑140 (MQ=255)

                                                                          cgcAAGGATAGATTTACCGCTACTGAGGGTTTCGGCAGCAGGAAACGCCTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGCATGGGCGTTg                                                               >  2:296446/1‑140 (MQ=255)

                                                                          cgcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTg                                                               >  1:448054/1‑140 (MQ=255)

                                                                          cgcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTg                                                               >  1:1225708/1‑140 (MQ=255)

                                                                          cgcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGGTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTg                                                               >  2:939523/1‑140 (MQ=255)

                                                                           gcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgc                                                              >  1:1751260/1‑140 (MQ=255)

                                                                                gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  2:1265307/140‑1 (MQ=255)

                                                                                gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         >  1:4224179/1‑140 (MQ=255)

                                                                                gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  1:525442/140‑1 (MQ=255)

                                                                                gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  1:2220914/140‑1 (MQ=255)

                                                                                gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  1:3192415/140‑1 (MQ=255)

                                                                                     attccccGATAGTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGAt                                                    <  2:2333851/135‑1 (MQ=255)

                                                                                     aTTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGAt                                                    <  1:3533865/140‑1 (MQ=255)

                                                                                         aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                                >  2:2892217/1‑140 (MQ=255)

                                                                                          ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCgg                                                                                                                  >  1:974221/1‑73 (MQ=255)

                                                                                          ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               >  1:232807/1‑140 (MQ=255)

                                                                                          ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               >  2:380635/1‑140 (MQ=255)

                                                                                          ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               >  2:1966636/1‑140 (MQ=255)

                                                                                           cGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGAc                                              >  2:1873051/1‑140 (MQ=255)

                                                                                              tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                                           <  2:4560686/140‑1 (MQ=255)

                                                                                              tACTGTGGGTGTCGGAAGCAGTCAACGCTTTGCCGTAGTAATGTTCCCATTTCACACACCAGGCACCGGCACTGACAACGCGCAACAAACGCGTGGGTTTCCACCTCTTAATGGTCGTTGTCGCCTGAGATGAAGACTGa                                           <  2:3459427/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAGTGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:3848122/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          >  1:2015498/1‑140 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:2854336/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:5461038/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:497433/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:2124253/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:4871012/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:1873051/140‑1 (MQ=255)

                                                                                               aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACCGAc                                          <  2:7633/140‑1 (MQ=255)

                                                                                               aCTATGGGTTTCGGCAGCAGTAAACGCATTGCCGTAGTAAGGTTCCCATTACACATCCCAGCCACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACGGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:2400842/140‑1 (MQ=255)

                                                                                                  gtgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAg                                       <  2:2944629/140‑1 (MQ=255)

                                                                                                   tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:4722384/1‑140 (MQ=255)

                                                                                                   tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:2099177/1‑140 (MQ=255)

                                                                                                   tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGGTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  2:36070/1‑140 (MQ=255)

                                                                                                     ggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGACAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCa                                    <  1:4086029/140‑1 (MQ=255)

                                                                                                     ggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCa                                    >  1:5347593/1‑140 (MQ=255)

                                                                                                        ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAg                                 >  1:280149/1‑140 (MQ=255)

                                                                                                          cGGCAGCAGTAGACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGAGGGTTGCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCAGAGCa                               <  1:4587896/140‑1 (MQ=255)

                                                                                                          cGGCAGCAGTAAGCGCTTTGCAGGAGTGAGGTGCCCATCTCACGTACCAGAGACCGGCAATGACAACGAGGAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACGGACCAGTCATAGCa                               <  2:2407535/140‑1 (MQ=255)

                                                                                                          cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  1:1600137/140‑1 (MQ=255)

                                                                                                          cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  2:118464/140‑1 (MQ=255)

                                                                                                               gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc                          <  1:1961108/140‑1 (MQ=255)

                                                                                                               gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc                          <  1:4386622/140‑1 (MQ=255)

                                                                                                               gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc                          <  2:3062751/140‑1 (MQ=255)

                                                                                                               gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc                          <  2:3236946/140‑1 (MQ=255)

                                                                                                                     aaCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGaa                    <  2:509683/140‑1 (MQ=255)

                                                                                                                      aCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAAt                   <  1:504206/140‑1 (MQ=255)

                                                                                                                          tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:1659438/140‑1 (MQ=255)

                                                                                                                          tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:4217276/140‑1 (MQ=255)

                                                                                                                          tctGCCGTAGTAAGGTACCCATCTCACATACCAGAGACCGGCAATGCCAACGAGAAAGAAAAGCGCCGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:4908739/138‑1 (MQ=255)

                                                                                                                           ttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGtt              >  1:660585/1‑140 (MQ=255)

                                                                                                                           ttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCCCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGtt              >  2:3969820/1‑140 (MQ=255)

                                                                                                                            tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             >  2:2768236/1‑140 (MQ=255)

                                                                                                                                      aGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGa   >  1:4011371/1‑140 (MQ=255)

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCAT‑GGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`