Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,479,488 T→C *602* (TAA→TAG)  kefB ← glutathione‑regulated potassium‑efflux system protein KefB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,479,4880TC99.6% 923.1 / NA 281*602* (TAA→TAG) kefBglutathione‑regulated potassium‑efflux system protein KefB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (176/104);  minor base A (0/1);  total (176/105)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCA  >  NZ_CP025268/3479352‑3479627
                                                                                                                                        |                                                                                                                                           
gCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTAc                                                                                                                                          >  2:693424/1‑140 (MQ=255)
 cATCTGATGTCCGCACTTAACACATTCCACGATATCAATACTCTTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGGCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                                                         <  2:2180071/140‑1 (MQ=255)
 cATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                                                         <  1:938012/140‑1 (MQ=255)
 cATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                                                         <  1:5224171/140‑1 (MQ=255)
  aTCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTc                                                                                                                                        <  1:3250818/140‑1 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                              >  2:1571331/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                              >  1:814516/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                              >  1:1294912/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                              >  2:1909186/1‑140 (MQ=255)
              cACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCa                                                                                                                            >  2:2855563/1‑140 (MQ=255)
              cACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCa                                                                                                                            >  2:2523233/1‑140 (MQ=255)
               aCTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCAt                                                                                                                                >  1:610339/1‑135 (MQ=255)
                cTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGc                                                                                                                          <  1:4154814/140‑1 (MQ=255)
                  tAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCg                                                                                                                        >  1:1149608/1‑140 (MQ=255)
                   aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGt                                                                                                                       >  1:875920/1‑140 (MQ=255)
                   aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGt                                                                                                                       >  1:3679437/1‑140 (MQ=255)
                    acacATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTc                                                                                                                      >  1:4357629/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAACTTCATCCCAGCCGTCCAGCt                                                                                                                 >  1:1530176/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAgat                                                                                                                 >  2:3377599/1‑138 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                                 <  1:1013046/140‑1 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                                 >  2:1781052/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                                 >  2:3779744/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                                 >  1:2689989/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                                 >  2:2273337/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                               >  1:2075368/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                               >  2:1148659/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                               >  2:3609290/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACAGCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                               >  2:4908560/1‑140 (MQ=255)
                                atatcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcg                                                                                                          <  1:5565323/140‑1 (MQ=255)
                                  atcaatatTATTTTCGCGCCACCTCGCCATTGAATCGTGCGCCTGACAGGCCGGGCATTTTGCGCGCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGGCCAGCTGGCGTCGtt                                                                                                        >  2:1891278/1‑140 (MQ=255)
                                  atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGtt                                                                                                        >  2:420204/1‑140 (MQ=255)
                                  atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGCTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGtt                                                                                                        >  2:2250654/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  2:4142003/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  1:857318/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  2:3282081/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  2:2706581/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  1:5352569/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  1:5199980/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                    >  1:4920871/1‑140 (MQ=255)
                                      atatTATTTTCGCGACACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCGTCTTTACCCTCTACTCAAATTCATCCCAGCCGCCCACCTGGCGTCGTTCttg                                                                                                    >  2:590398/1‑140 (MQ=255)
                                      agattattTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTGAAATTCATCCCATCCGTCCAGCTGGCGTCGTTCttt                                                                                                    >  1:4481172/3‑139 (MQ=255)
                                         ttattTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGGCCAGCTGGCGTCGTTCttgttg                                                                                                 >  2:3132917/1‑140 (MQ=255)
                                         tgattTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTGTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttgttg                                                                                                 <  1:3805546/138‑1 (MQ=255)
                                                 gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTTGGATTGCCGTCTTTACCCTCTACTCAAATTCATCCCAGGCGTGCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                         >  2:3179339/1‑140 (MQ=255)
                                                 gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCTATCTTTACCCTCTACTCAAATTCCTCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                         >  2:5495901/1‑140 (MQ=255)
                                                 gcgcCACATCGCCATTGAAGCCTGCGCCTGACAGGCCGGGCCTTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTCCCCTCTACTCAAATTCATCCCAGCCGTACAGCTGGCGTCGTTCTTGTTTCATTTCAc                                                                                         >  2:3870884/1‑140 (MQ=255)
                                                               ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATctct                                                                           >  2:3601626/1‑140 (MQ=255)
                                                               ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATctct                                                                           >  1:49775/1‑140 (MQ=255)
                                                                 gAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTc                                                                         >  2:2012493/1‑140 (MQ=255)
                                                                 gAATCCTGCGCCTCACAGGCCGCGCATTTTGCGCCCGCAATAAACGGTTTTCGGATTGCCATCTTTCCTCCCTACTCAAATGCATCCCAGCCGGCCAGCCGGCGTCGGTTTTGTTGCATTTCACCCTGGAAAATCACTTc                                                                         >  2:1520110/1‑140 (MQ=255)
                                                                    tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAg                                                                      >  1:695241/1‑140 (MQ=255)
                                                                    tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAAGAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCGAGCTGGCGTCGTTCTTGTTTCATTTCACGCTGGAAAATCTCTTCCAg                                                                      >  1:1167797/1‑140 (MQ=255)
                                                                       tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCCCGCTGGAAAATCTCTTCCAGTTc                                                                   >  2:2761738/1‑140 (MQ=255)
                                                                       tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                                                   >  1:2565684/1‑140 (MQ=255)
                                                                       tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                                                   >  2:3678916/1‑140 (MQ=255)
                                                                       tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                                                   >  2:1019438/1‑140 (MQ=255)
                                                                         ggcCTGACACGCCGGCCATTTTGAGCCCGGAATAAAACGTTTTCGCATTGCCATCTTTACCCTCTACTCAAATTCCTCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCTTTTCACGCTGGAAAATCTCTTCCTGTTcgc                                                                 >  2:5418121/2‑140 (MQ=255)
                                                                         cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcgg                                                                 >  2:3002730/1‑139 (MQ=255)
                                                                         cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcgc                                                                 >  2:95459/1‑140 (MQ=255)
                                                                             tGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTCCCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                                                             <  1:2995721/140‑1 (MQ=255)
                                                                              gACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCgg                                                            >  1:1636745/1‑140 (MQ=255)
                                                                              gACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCgg                                                            >  1:4869200/1‑140 (MQ=255)
                                                                               aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                                           <  2:2453552/140‑1 (MQ=255)
                                                                               aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                                           <  1:4912148/140‑1 (MQ=255)
                                                                                   gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTc                                                       >  1:1051909/1‑140 (MQ=255)
                                                                                   gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTc                                                       >  1:758731/1‑140 (MQ=255)
                                                                                        gCATTTTGCGCCCGCAAAATAACGGTTTCGGATTGCCATCTTTACCCTCTACTCATATTCATGCCATCCGTCCAGCTGGCGTCGTTCTTTTTGCATTTGTCGCTGGAAACTCTCTTCCAGTTCGCGTCGGGCTTCCCTgg                                                  >  2:3923129/1‑140 (MQ=255)
                                                                                             ttGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga                                             <  1:2283871/140‑1 (MQ=255)
                                                                                             ttGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga                                             <  1:3728040/140‑1 (MQ=255)
                                                                                              tGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCgag                                            >  1:2252154/1‑140 (MQ=255)
                                                                                                    cGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAttt                                      >  2:793598/1‑140 (MQ=255)
                                                                                                      cAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGt                                    >  1:1716354/1‑140 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                                   <  1:545301/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                                   <  2:1535579/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                                   <  1:3594976/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                                   <  1:454561/140‑1 (MQ=255)
                                                                                                          aaaaCGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACgg                                <  1:4883051/140‑1 (MQ=255)
                                                                                                            aaCGTATTCGGATTGCCATCTTTACCTTCTACTCAAATTCATCCCAGCCGTACAGCTGGCGTCGTTCGTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTa                              <  2:5095374/140‑1 (MQ=255)
                                                                                                               tttttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCATCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCg                           <  2:4307798/139‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCCAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         <  1:2905310/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAGATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         <  1:3818654/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         <  1:3639587/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         >  1:1403199/1‑140 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         <  1:346594/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         <  1:2134576/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         <  1:1872610/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                         >  1:1902274/1‑140 (MQ=255)
                                                                                                                   tCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcat                       <  2:1623973/140‑1 (MQ=255)
                                                                                                                      gATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCCTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgca                    <  1:2469748/140‑1 (MQ=255)
                                                                                                                      gATTGCCATCTTTACCCTATGCTACAATACATCCCAGCCGTCCAGATGGCGTCGTTCTTGCTGCAGTTCTCGCTGGAAAATAACTAACAGTTCGCGTCGGGCATCCCTGGAGCGAGAAAATTGTACGGTATCGgcatgca                    <  2:3460233/140‑1 (MQ=255)
                                                                                                                       aTTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgcat                   <  1:4794620/140‑1 (MQ=255)
                                                                                                                       aTTACCATCTTCACCGTCTACTAGAATTCATCCCAGCCGTCCAGCTTGCTTCGTGCTTGTTGCATTTCACGCCGAAAGATCTCTTCTTGTTCTCGTCGGCCTTCCCTGTCGCGAGATATTTGTACGGTATCGgcatgcat                   <  2:5190983/140‑1 (MQ=255)
                                                                                                                         tGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTg                 >  1:4093877/1‑140 (MQ=255)
                                                                                                                          gCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt                                        >  2:5235735/1‑116 (MQ=255)
                                                                                                                            cATCTTTACCCTCTCCTCAAATTCCTCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGa              <  2:1317002/140‑1 (MQ=255)
                                                                                                                              tCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATg            <  2:4864367/140‑1 (MQ=255)
                                                                                                                              tCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATg            <  1:2006588/140‑1 (MQ=255)
                                                                                                                               cTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGa           <  2:2836263/140‑1 (MQ=255)
                                                                                                                                tttACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAg          <  1:3911774/140‑1 (MQ=255)
                                                                                                                                    cccTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct      <  2:456087/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCA  >  NZ_CP025268/3479352‑3479627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑