Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,508,628 G→A pseudogene (648/878 nt) php ← phosphotriesterase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,508,6280GA99.7% 1411.4 / NA 381pseudogene (648/878 nt)phpphosphotriesterase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (247/132);  minor base C (1/0);  total (249/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 390 total aligned reads displayed.

AGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTAGC  >  NZ_CP025268/3508492‑3508764
                                                                                                                                        |                                                                                                                                        
aGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGgtagta                                                                                                                                       >  2:293195/1‑140 (MQ=255)
aGTAAATAGTCATAGACATAACCACCGTTGTCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGgtagta                                                                                                                                       >  2:4728548/1‑140 (MQ=255)
    aaTAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGAAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACGCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTg                                                                                                                                   >  2:4923167/1‑140 (MQ=255)
     aTAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGt                                                                                                                                  >  1:1676500/1‑140 (MQ=255)
       aGTCATAGCCATAACCACCGTTGGCTTTAAAATGGGAGCGGCGCGAAATAACCAACGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAACTCATGAAGCACCGCAATACGCTTTTCGTCCGGGAAGTATCTGTTc                                                                                                                                >  2:823023/1‑140 (MQ=255)
        gTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCt                                                                                                                               >  2:5422510/1‑140 (MQ=255)
        gTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCt                                                                                                                               >  2:1949534/1‑140 (MQ=255)
        gTCATAGCCATAACCACCGCTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCt                                                                                                                               >  1:5041904/1‑140 (MQ=255)
           aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGc                                                                                                                            >  2:4073043/1‑140 (MQ=255)
           aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGc                                                                                                                            >  2:1484198/1‑140 (MQ=255)
            tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                           >  2:189318/1‑140 (MQ=255)
            tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                           >  1:896714/1‑140 (MQ=255)
            tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                           >  1:2192908/1‑140 (MQ=255)
              gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                         <  1:1406395/140‑1 (MQ=255)
                   aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                    >  1:1093830/1‑140 (MQ=255)
                   aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                    >  2:1564743/1‑140 (MQ=255)
                   aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                    >  1:775529/1‑140 (MQ=255)
                   aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                    <  1:4145934/140‑1 (MQ=255)
                   aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                    >  1:3536658/1‑140 (MQ=255)
                   aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                    >  1:11487/1‑140 (MQ=255)
                   aaCCAAAGTTGGCCTTTAAATGGGAGCGACGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGGAGTAACTGTTCTTGCCGATtgtg                                                                                                                    >  2:5329438/1‑140 (MQ=255)
                        ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                               <  1:3214169/140‑1 (MQ=255)
                        ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                               <  2:328421/140‑1 (MQ=255)
                         cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                              >  1:5598065/1‑140 (MQ=255)
                                ttttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGt                                                                                                       >  1:987794/1‑140 (MQ=255)
                                 tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCCTGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                      <  1:5522059/140‑1 (MQ=255)
                                 tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                      <  2:2067203/140‑1 (MQ=255)
                                 tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                      <  2:1268300/140‑1 (MQ=255)
                                 tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                      <  2:3426252/140‑1 (MQ=255)
                                 tttAAATGGGAGAGCCGCGTAATATCCATCGCCAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGAATGAAGCATCGCAATACGCTTTTCGTACGGGTAGTAAATGTTCTTGCCGATGGTGTCGAACTGCCCGTa                                                                                                      <  2:4350108/140‑1 (MQ=255)
                                   tAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTAcg                                                                                                    >  1:3960745/1‑140 (MQ=255)
                                   tAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTAcg                                                                                                    >  1:5433607/1‑140 (MQ=255)
                                         ggAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCga                                                                                              >  1:4727608/1‑140 (MQ=255)
                                          gcgcggcgCGTAATATCCATCGACAGCATGACGCGGGTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATCCGCTTTTCGTCCGGGTAGTAACTGTGCGTGCCGATGGTGTCGAACTGCACGTACTCGCCgag                                                                                             >  2:2575294/3‑140 (MQ=255)
                                          gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCgag                                                                                             >  2:354577/1‑140 (MQ=255)
                                             cggcgCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatc                                                                                          >  1:2974758/1‑140 (MQ=255)
                                                cgcgTAACATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgat                                                                                       <  1:125009/140‑1 (MQ=255)
                                                 gcgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                                      <  1:4444987/140‑1 (MQ=255)
                                                         tCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaa                                                                              >  1:3679148/1‑140 (MQ=255)
                                                         tCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaa                                                                              >  2:5110875/1‑140 (MQ=255)
                                                          ccATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                             >  1:4875895/1‑140 (MQ=255)
                                                          ccATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                             >  1:5104689/1‑140 (MQ=255)
                                                          ccATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                             >  1:5169184/1‑140 (MQ=255)
                                                           cATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                            <  1:3639469/140‑1 (MQ=255)
                                                           cATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGAGGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                            >  1:2069222/1‑140 (MQ=255)
                                                            aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTGGTCCGGGTAGTAACTGTTCTTTCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                           >  1:420628/1‑140 (MQ=255)
                                                            aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                           >  1:4515810/1‑140 (MQ=255)
                                                            aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                           >  2:742631/1‑140 (MQ=255)
                                                             tCGACAGCATGACGCGGTGCCGCCATCCACGGCCGCCTAGCGCCTGAACCATCGCAGTACGCCTTTCCTTCCAGTACGAACTGTTCTTGACGAACGCGTCGACCTTCGAGTCCACGCCGGGCCCGGTCATCGTCAAAAtg                                                                          >  2:5162624/1‑140 (MQ=255)
                                                              cGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGGTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtgt                                                                         >  2:4451305/1‑140 (MQ=255)
                                                               gACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGACCATCTTCAAAAtgtt                                                                        >  2:4145754/1‑140 (MQ=255)
                                                                 cAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATATTCAAAAtgttgt                                                                      >  2:4860333/1‑140 (MQ=255)
                                                                    cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGTAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACACGCCGAGATCGATCATCTTCAAAATGTTGTCGa                                                                   >  2:3094007/1‑140 (MQ=255)
                                                                    cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGAGGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAGGTTGTCGa                                                                   >  2:59929/1‑140 (MQ=255)
                                                                     aTGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAg                                                                  >  1:183286/1‑140 (MQ=255)
                                                                      tGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                                 <  1:4043421/140‑1 (MQ=255)
                                                                        ccgcaGTTCAACAACCCACGGTCGCGTAGCGCACGAACCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                               <  2:4315816/139‑1 (MQ=255)
                                                                         cgcgGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTg                                                              <  1:941840/140‑1 (MQ=255)
                                                                           cgGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTGGCCGATGGGGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTc                                                            <  1:4362302/140‑1 (MQ=255)
                                                                              ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAAGTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTGGTGttt                                                         >  1:4087039/1‑140 (MQ=255)
                                                                              ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                         >  2:4269099/1‑140 (MQ=255)
                                                                                  gCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACCGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGCTGTCTTTCAGa                                                     <  1:4637502/140‑1 (MQ=255)
                                                                                     aCCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCg                                                  <  1:2636856/140‑1 (MQ=255)
                                                                                       ccACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                                <  1:1576776/140‑1 (MQ=255)
                                                                                       ccACGGTCGCGTAGAGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGTTGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                                <  2:2759946/140‑1 (MQ=255)
                                                                                        cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTTTCGAGGTTGTCTTTCAGATCGCAg                                               >  2:1732655/1‑140 (MQ=255)
                                                                                          cGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTg                                             >  2:5495371/1‑140 (MQ=255)
                                                                                          cGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTg                                             >  1:5464798/1‑140 (MQ=255)
                                                                                           ggTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGa                                            >  1:1223760/1‑140 (MQ=255)
                                                                                               gcgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCaa                                        <  1:215051/140‑1 (MQ=255)
                                                                                                cgTAGCGCATGAAGCATCGCAATGCGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCGTCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                       >  1:3361887/1‑140 (MQ=255)
                                                                                                cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                       >  1:2012325/1‑140 (MQ=255)
                                                                                                cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                       >  2:3664464/1‑140 (MQ=255)
                                                                                                cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                       >  2:2005996/1‑140 (MQ=255)
                                                                                                cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                       >  2:3931908/1‑140 (MQ=255)
                                                                                                          gAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcgcgc                             <  1:3860203/140‑1 (MQ=255)
                                                                                                            aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                           <  1:1039530/140‑1 (MQ=255)
                                                                                                            aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                           <  1:1345775/140‑1 (MQ=255)
                                                                                                            aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                           <  2:3596472/140‑1 (MQ=255)
                                                                                                            aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                           <  2:31886/140‑1 (MQ=255)
                                                                                                            aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                           <  2:2347341/140‑1 (MQ=255)
                                                                                                               aTCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATTTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGGGACGCGGGAAAg                        >  1:2485087/1‑140 (MQ=255)
                                                                                                               aTCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAg                        >  1:3160372/1‑140 (MQ=255)
                                                                                                                 cGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGAt                      >  2:3919046/1‑140 (MQ=255)
                                                                                                                 cGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGAt                      >  1:5314584/1‑140 (MQ=255)
                                                                                                                   cAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCa                    <  2:2494543/140‑1 (MQ=255)
                                                                                                                      tACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAAcc                 >  1:2665906/1‑140 (MQ=255)
                                                                                                                      caCGCTTTTCGTCCGGGTAGTGACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCAGAGATGGATCATCTTCAAAATGTTGTCGAGGTTGTCTTGCAGATCGAAGTAACCAACGGTGAAGCGCGAAAGATCAAcc                 >  2:4645271/2‑140 (MQ=255)
                                                                                                                          cTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGt             <  1:1483570/140‑1 (MQ=255)
                                                                                                                          cTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGt             <  2:3370225/140‑1 (MQ=255)
                                                                                                                          cTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGt             <  2:4017435/140‑1 (MQ=255)
                                                                                                                           ttttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGGGGTTGTCTTTCAGATCGCAGTGACCAACGGGGACGCGCGAAAGATCAACCCCGTg            >  2:5245184/1‑140 (MQ=255)
                                                                                                                           ttttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGCTCAACCCCGTg            >  2:5557121/1‑140 (MQ=255)
                                                                                                                           ttttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTg            >  1:2374741/1‑140 (MQ=255)
                                                                                                                           ttttCGTCCGGGTAGTAACTGTTCTTGCCGATGGGGTCGAACTGCACGTACGCGCCGAGATCGATCCTCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCACCGGTGACGCGCGAAAGCTCAACCCCGTg            >  1:2459305/1‑140 (MQ=255)
                                                                                                                             ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTTCACGTACGCGCCGAGATCGAGCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACTAACGTTGACGCGCGAAAGAGCAACCCCGTggg          >  2:226446/1‑140 (MQ=255)
                                                                                                                             ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCTCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTggg          >  2:3725650/1‑140 (MQ=255)
                                                                                                                             ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTggg          >  1:2906829/1‑140 (MQ=255)
                                                                                                                             ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTGCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTggg          >  2:4247289/1‑140 (MQ=255)
                                                                                                                                     ggtagtAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAAGTTGTCGAGGTTGTCTTTCAGATCGCAGTGCCCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTacc  >  2:5051986/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTAGC  >  NZ_CP025268/3508492‑3508764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑