Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,516,790 C→T K401K (AAG→AAA)  damX ← cell division protein DamX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,516,7900CT99.7% 1227.0 / NA 334K401K (AAG→AAA) damXcell division protein DamX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (152/181);  minor base G (0/1);  total (152/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

AGCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAG  >  NZ_CP025268/3516654‑3516926
                                                                                                                                        |                                                                                                                                        
 gcgcTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTtttt                                                                                                                                      >  2:3448328/1‑140 (MQ=255)
     ttCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGc                                                                                                                                  >  2:637714/1‑140 (MQ=255)
        aGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCctc                                                                                                                               <  2:1460953/140‑1 (MQ=255)
              cTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCttt                                                                                                                         <  1:135154/140‑1 (MQ=255)
               tCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTc                                                                                                                        >  2:3146995/1‑140 (MQ=255)
                    cAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGc                                                                                                                   >  1:215150/1‑140 (MQ=255)
                       cGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                                <  2:5310940/140‑1 (MQ=255)
                        gATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTac                                                                                                               >  1:1107763/1‑140 (MQ=255)
                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTaca                                                                                                              <  1:1278410/140‑1 (MQ=255)
                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTaca                                                                                                              <  1:1706271/140‑1 (MQ=255)
                           aaaCCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACg                                                                                                            >  2:1145788/1‑140 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  1:2881323/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  1:4044064/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  2:3288507/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  2:3758679/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  1:130234/140‑1 (MQ=255)
                                   gCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                                    <  1:5152890/140‑1 (MQ=255)
                                    cGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAcc                                                                                                   <  2:3814520/140‑1 (MQ=255)
                                      ggAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAg                                                                                                 >  1:4902089/1‑140 (MQ=255)
                                       gagaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAgg                                                                                                >  2:4171975/1‑140 (MQ=255)
                                        agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGTTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  2:4890820/140‑1 (MQ=255)
                                        agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  2:1954825/140‑1 (MQ=255)
                                        agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  2:835933/140‑1 (MQ=255)
                                        agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  1:2415431/140‑1 (MQ=255)
                                        agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  1:1494692/140‑1 (MQ=255)
                                         gaTCACCTTGAGTACTTCAGATCGGCCGGTGCCTGGCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGGAGCTACCGCTTGTGTCGCCTCTTCTTTCGAAACGTACTCGCCAGAAACCCGGAc                                                                                              >  2:1545075/1‑140 (MQ=255)
                                         gaTAACCTTGATTACTTCAGATCGGCCTGTACCTGTCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGAc                                                                                              >  2:4772128/1‑140 (MQ=255)
                                             aCCTTGATTACTTCAGATCGGCCTGTACCTGCCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATAc                                                                                          <  2:2918345/140‑1 (MQ=255)
                                             aCCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATAc                                                                                          <  1:3397917/140‑1 (MQ=255)
                                               cTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                        <  1:5093455/140‑1 (MQ=255)
                                                 tGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACg                                                                                      >  2:1607753/1‑140 (MQ=255)
                                                 tGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACg                                                                                      <  2:4435488/140‑1 (MQ=255)
                                                  gATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCACTGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                                     <  1:101623/140‑1 (MQ=255)
                                                  gATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                                     >  1:4184211/1‑140 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGGTTGCAATGTAGATACCGCTTTTTTCGCCTCTTCGTTCGAAGCGTACACGCCAGAAACGTGGACATACCActgg                                                                                    >  2:4278859/1‑137 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    <  1:5282200/140‑1 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  2:3305243/1‑140 (MQ=255)
                                                    ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  1:3510412/140‑1 (MQ=255)
                                                    ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  1:4596967/140‑1 (MQ=255)
                                                    ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  1:2007093/140‑1 (MQ=255)
                                                    ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTGTTCGCCTCTTCTTTCGACGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  2:2184718/140‑1 (MQ=255)
                                                        ttCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGAcc                                                                               >  2:4334868/1‑140 (MQ=255)
                                                        ttCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCGCTTCTTTCGAAGCGTACACGCCAGAAACCAGTACATACCACGGCTGAcc                                                                               >  2:1398776/1‑140 (MQ=255)
                                                         tCAGGTCGGCCTGTACCTGACGCAGCGGCCTCGCCCACGGGTTTTTTGCCTGGACATCTGCTGGCAATGTAGGTACCGCTTTTTTCGCCTGTTCTTACTAAGCGTACACGCCAGAAACCAGGACATACCACGGCAGAccc                                                                              >  2:5147424/1‑139 (MQ=255)
                                                         tCAGATCGGCCTGTACCTGACGCAGCTGTTTCGCCCACGTGTTTTTGGCCTGGACATCTGCTGGCAAGGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGGGTACACGCCAGAAACCAGGACATACCACGGCTGAcct                                                                              >  1:225779/1‑139 (MQ=255)
                                                         tCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCa                                                                              >  2:2681815/1‑140 (MQ=255)
                                                             aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAc                                                                          >  1:2728760/1‑140 (MQ=255)
                                                              tCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcg                                                                         <  2:2281774/140‑1 (MQ=255)
                                                                ggCCTGTGCCTGGCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGAGACCGCTTTTTTCGCCTCTTCGTTCGACGCGTACGCGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  2:2412353/140‑1 (MQ=255)
                                                                ggCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  2:818445/140‑1 (MQ=255)
                                                                ggCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  1:4978824/140‑1 (MQ=255)
                                                                ggCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  2:103921/140‑1 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcggc                                                                     >  1:4776287/1‑138 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     >  2:3059261/1‑140 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  2:1231658/140‑1 (MQ=255)
                                                                           gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCGGGACATCTGCTGGCCATGTAGATACTGCTTTTTTCGCCTCTTCTTGCGAAGCGTACACGCCAGGAACCAGGACATACCACGGCTGGCCATTACGCGTCGTGTAATAg                                                            >  2:4567286/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  2:2519620/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:640075/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:1457838/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTGTGGCCTGGACATCTGCTGGCAATGTAGGTACCGCTGGGTTCGCCTCTCCTTCCGAAGCGTACACGCCAGAAACCAGGAGATACCGCTGCGGACCATTACGCGTCGTTTCATAGa                                                           >  2:4530164/1‑140 (MQ=255)
                                                                               cAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACaa                                                        <  2:1996399/140‑1 (MQ=255)
                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  1:5491662/1‑140 (MQ=255)
                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCCGACCATTACGCGTCGGTTCAGAGACAAc                                                       >  2:1914593/1‑140 (MQ=255)
                                                                                  cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGt                                                     >  1:2528717/1‑140 (MQ=255)
                                                                                             aCGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGa                                          <  1:4382034/140‑1 (MQ=255)
                                                                                             aCGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGa                                          <  2:1297908/140‑1 (MQ=255)
                                                                                             aCGGGTTTTTGGCCTGGAAATCTGGCGGCACTGTAGATACCGCTTTTTTCGCCTCTTCTCTCGACGCGTACACGTCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGa                                          <  2:3929763/140‑1 (MQ=255)
                                                                                                     ttGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtcttt                                  <  2:2237697/140‑1 (MQ=255)
                                                                                                     ttGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtcttt                                  <  2:4059067/140‑1 (MQ=255)
                                                                                                     ttGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtcttt                                  <  1:849163/140‑1 (MQ=255)
                                                                                                     ttGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTCCGATGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtcttt                                  <  1:3981500/140‑1 (MQ=255)
                                                                                                           tGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGc                            >  1:2081513/1‑140 (MQ=255)
                                                                                                           tGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGc                            >  2:2745845/1‑140 (MQ=255)
                                                                                                                  ctgctgGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCg                     >  2:2122946/1‑140 (MQ=255)
                                                                                                                  cggctgGCAATGTAGATACCGCTTTTTTCGCCTCATCTTTCGAAGCGTACACGCCAGAAACCAGGACATACTACGGCTGACCATTACGCGTCGTTTCATAGACAACGTCGTTTTTCAGATTGTCTTTCTTCGCCCAACCg                     >  2:5012478/3‑140 (MQ=255)
                                                                                                                    gctgGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGtt                   <  1:2519620/140‑1 (MQ=255)
                                                                                                                    gctgGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGtt                   >  2:5588942/1‑140 (MQ=255)
                                                                                                                       ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  1:3724000/1‑140 (MQ=255)
                                                                                                                       ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  2:2557400/1‑140 (MQ=255)
                                                                                                                        gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:1378700/140‑1 (MQ=255)
                                                                                                                        gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:3839066/140‑1 (MQ=255)
                                                                                                                        gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:4268589/140‑1 (MQ=255)
                                                                                                                        gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:2680032/140‑1 (MQ=255)
                                                                                                                            tGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGt           >  1:719507/1‑140 (MQ=255)
                                                                                                                            tGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATGACGCGTCGTTTCATAGAGAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTGCAGGTTGt           >  1:301784/1‑140 (MQ=255)
                                                                                                                             gTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATCCCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTc          <  1:1577260/140‑1 (MQ=255)
                                                                                                                             gTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTGGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCTAACCGTTCAGGTTGTc          >  1:3115022/1‑140 (MQ=255)
                                                                                                                             gTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTc          <  1:1882741/140‑1 (MQ=255)
                                                                                                                              tAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCg         <  1:3201187/140‑1 (MQ=255)
                                                                                                                              tAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCg         <  2:2093346/140‑1 (MQ=255)
                                                                                                                              tAGATACCGCTGTTTGCTCCTCTTCTTTCGAAGCGTACACGCCAGAGACCGGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCg         <  2:3665557/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTTTTCTTTCGAATCGTGCTCGCCAGAAACCAGAACATACCACGGCTGAACAGTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTCTCGTa       <  2:697326/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCCCGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  1:1740928/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  2:4917042/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  2:1715660/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  1:1193134/140‑1 (MQ=255)
                                                                                                                                  tACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGt     >  1:5336798/1‑140 (MQ=255)
                                                                                                                                  tACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGt     >  1:664038/1‑140 (MQ=255)
                                                                                                                                   aCCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGtt    >  1:5207695/1‑140 (MQ=255)
                                                                                                                                     cGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTCTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTag  <  1:3919446/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AGCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAG  >  NZ_CP025268/3516654‑3516926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑