BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	353,643	A→G	629 (TAA→TAG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	353,643	0	A	G	99.4%	1135.6 / ‑6.1	342	629 (TAA→TAG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (120/219); total (121/221)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

CAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NZ_CP025268/353506‑353777

 aaTTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGtt                                                                                                                                     <  1:45344/140‑1 (MQ=255)

     gCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTg                                                                                                                                 >  1:3584176/1‑140 (MQ=255)

      ccAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAGAGACGCGACCCTGGTGCTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCCCTAGGCGATGGAAGCGTAGGTTATTGt                                                                                                                                >  2:1967251/1‑140 (MQ=255)

         aaaaCGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGAGCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGACGAGTAGGTTATTGTCgg                                                                                                                             >  2:1474601/1‑140 (MQ=255)

                gATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTc                                                                                                                      >  1:5346241/1‑140 (MQ=255)

                 aTCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcg                                                                                                                     <  2:2375261/140‑1 (MQ=255)

                   ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcg                                                                                                                   <  1:1678511/140‑1 (MQ=255)

                   ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcg                                                                                                                   <  2:1534649/140‑1 (MQ=255)

                     ggAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcgcg                                                                                                                 <  2:552675/140‑1 (MQ=255)

                       caaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGCTCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  1:3710684/139‑1 (MQ=255)

                       agaaaTGCTGCGGCTGAAGCTGCAGGCGATTTGCGGAGGACGCGATCCTGGAGATGTGAGGGCCAGTGATGAGCCTGCGCTGTTGGAGGAGATTCGCCGGCCGCTGGAGGAGTGAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:5007287/138‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCAGGAACAGGGCGATTTGCGAAGGACGGCCTCCTGGAGAACTGACGACCATTGATGATCCTGCGTCGTTGTATCAGATCCGCCAGGCGATGGATGAGTAGGTTGTTGACGGGTGCGTCGCGCGGt                                                                                                               <  2:292245/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  1:2356942/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  1:331051/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:1380800/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  1:3176679/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:3560679/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  1:2906153/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:4121192/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  1:584459/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:4974114/140‑1 (MQ=255)

                            tgctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATc                                                                                                          <  2:562663/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  1:2634111/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  1:1604515/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  1:4734767/140‑1 (MQ=255)

                                 cgccgcACGAGCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATACTGCGTCGGTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTGATTGTCGGAGGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  2:1002701/140‑1 (MQ=255)

                                     acACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgt                                                                                                 <  1:4931604/139‑1 (MQ=255)

                                          tccgaGGCGATTTTCGAAGGACGCGATCCTGGAGATCTGACGTCCATTGATGTTCGTGCGTCGTTGGATCAGATCCGCTAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGTGGTGCATCCGGGACTGTGTGcc                                                                                            <  2:2467353/136‑1 (MQ=255)

                                           tCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCg                                                                                           >  2:482293/1‑140 (MQ=255)

                                              aGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATg                                                                                        >  2:5391915/1‑140 (MQ=255)

                                                gCGATTTGCGAAGGACGCGCTCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGCTGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGcc                                                                                      <  2:2577300/140‑1 (MQ=255)

                                                gCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGcc                                                                                      <  2:3631017/140‑1 (MQ=255)

                                                   aTTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGa                                                                                   >  1:1104421/1‑140 (MQ=255)

                                                    tttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  1:3000059/1‑140 (MQ=255)

                                                     ttGCGAAGTACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCTTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  2:1210888/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGCTCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  2:2748920/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATGCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGGGCGGTGCATCCGGCAGTGTGTGCCGATGCCTGGTg                                                                                 >  2:3299419/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  1:3401607/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  1:4596788/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  1:4316380/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  1:3511711/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  2:3425295/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  2:4189964/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  1:1627205/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  2:618739/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                 >  2:900055/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATACTGGAGATCTGACGACCATTAATAATAATGCTTCGTTGAATCAGATCCGCCCGGCGATGGAAGAGACGGTTATTCTCGGATGCATCGCGCGGAGCATGCCGCACCGTGAGCCAATGCCTGATg                                                                                 >  2:1107204/1‑140 (MQ=255)

                                                              aCGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        <  2:5260909/140‑1 (MQ=255)

                                                               cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       <  1:1922510/140‑1 (MQ=255)

                                                               cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       <  1:2759186/140‑1 (MQ=255)

                                                                   ctCCTGGAGATCTGACGACCATTGATGATGCTGCGTCGTTGGATCGGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   <  2:2752933/139‑1 (MQ=255)

                                                                   aTCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   <  1:4139385/140‑1 (MQ=255)

                                                                   aTCCTGGAGATCTGAAGACCATTGATGATGCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGt                                                                   <  2:3050105/140‑1 (MQ=255)

                                                                          agaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCa                                                            <  2:3847928/140‑1 (MQ=255)

                                                                           gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                           <  1:794604/140‑1 (MQ=255)

                                                                           gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                           <  2:3308126/140‑1 (MQ=255)

                                                                           gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                           <  2:1702161/140‑1 (MQ=255)

                                                                             tCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc                                                         <  1:1413167/140‑1 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                        >  1:3238595/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                        >  2:2244216/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                        >  2:4574735/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                        >  1:5282753/1‑140 (MQ=255)

                                                                                gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  2:5202928/140‑1 (MQ=255)

                                                                                gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  2:3808139/140‑1 (MQ=255)

                                                                                gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  1:4624335/140‑1 (MQ=255)

                                                                                gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                      <  1:3648329/140‑1 (MQ=255)

                                                                                     cccttcatgatTCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGGAGAGTAGGTTATCGTCGGATCCCTCGCGCGGTGCACCCGGCACTGTGTGCCGCTGCCTCATGCGACGCTGACGCGTTTTATCATGCCTACGGAc                                                 <  2:106241/134‑1 (MQ=255)

                                                                                     ccATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGAc                                                 <  2:469691/140‑1 (MQ=255)

                                                                                     ccATTGATGATCCTGCGTCGTTGGACCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGAc                                                 <  2:3706827/140‑1 (MQ=255)

                                                                                     ccATTAATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGAc                                                 <  2:2972318/140‑1 (MQ=255)

                                                                                      cATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGAcc                                                >  1:1198416/1‑140 (MQ=255)

                                                                                       aTTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCt                                               <  1:72705/140‑1 (MQ=255)

                                                                                        ttGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                              <  2:4335650/140‑1 (MQ=255)

                                                                                        ttGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                              >  1:4978247/1‑140 (MQ=255)

                                                                                          gatgatCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGaa                                            <  1:3949980/140‑1 (MQ=255)

                                                                                             gatCCTGCGTCGTTGGATCAGATCCGGCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCg                                         <  2:1820417/140‑1 (MQ=255)

                                                                                             gatCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCg                                         <  1:1486066/140‑1 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                      <  1:1311721/140‑1 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGAGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                      >  1:3112009/1‑140 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGAAGGTTATTGTCGGAGGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                      >  2:4051789/1‑140 (MQ=255)

                                                                                                 cTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAgg                                     <  1:2139704/140‑1 (MQ=255)

                                                                                                 cTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAgg                                     <  2:312007/140‑1 (MQ=255)

                                                                                                  tGCGTCGTTGGATCCGATCCGCCAGGCGACGGAAGAGCAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                    >  1:5491414/1‑140 (MQ=255)

                                                                                                     gtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCgg                                 >  2:4758192/1‑140 (MQ=255)

                                                                                                                     cGCCAGGCGATGGAAGAGTAGGGTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                 <  1:710105/140‑1 (MQ=255)

                                                                                                                       ccAGGCGATGGAAGAGTAGGTTGTGGTCGGATGCGTCGCGCGGTACATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGc               <  2:428529/140‑1 (MQ=255)

                                                                                                                       ccAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGc               >  2:3194287/1‑140 (MQ=255)

                                                                                                                          ggCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATc            <  1:900066/140‑1 (MQ=255)

                                                                                                                          ggCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATc            <  1:1155499/140‑1 (MQ=255)

                                                                                                                           gCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGGACCGTAGGTCGAATAAGGCGCTCGCGTCGCATcc           >  2:733212/1‑140 (MQ=255)

                                                                                                                           gCGATGGAAGAGTAGGTTAGTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGCCCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATcc           <  2:1875942/140‑1 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg          >  1:3559949/1‑140 (MQ=255)

                                                                                                                               tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:2530629/140‑1 (MQ=255)

                                                                                                                               tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCAGCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  2:2124925/140‑1 (MQ=255)

                                                                                                                               tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGGGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       >  1:4391098/1‑140 (MQ=255)

                                                                                                                               cggAAGAGTAGGTTCTTGTCGGAGGCGTCGCGCGGTGCATCCGGCACTGTGTGCGGATGCGTGATGCGGCGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGGCGGCTAAGGCGCTCGCGTCGCACCCGacc       <  2:2031074/139‑2 (MQ=255)

                                                                                                                                ggAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGCCGCATCCGacac      >  2:3941467/1‑140 (MQ=255)

                                                                                                                                 gAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACAcc     <  1:2242772/140‑1 (MQ=255)

                                                                                                                                 gAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACAcc     <  1:5161133/140‑1 (MQ=255)

                                                                                                                                    gagTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGAAACCATg  <  1:1858538/140‑1 (MQ=255)

CAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NZ_CP025268/353506‑353777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`