BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,743,921	T→C	I172I (ATT→ATC)	galK →	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,743,921	0	T	C	99.3%	960.2 / NA	292	I172I (ATT→ATC)	galK	galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (149/140); minor base G (1/1); total (150/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

tAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATg                                                                                                                                      >  1:4747001/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:5002900/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:5543805/1‑140 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:5323662/140‑1 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:3768132/140‑1 (MQ=255)

        tcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTGAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCt                                                                                                                              >  1:2868252/1‑140 (MQ=255)

         ccGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTGTATCATCTGCCGCTGGACGGCGCCCAACTCGCGCTTAACGGTCAGGAAGCAGAAAACCAGGTTGTAGGCTGTAACTGCGGGATCCTGGATCAGcca                                                                                                                             >  2:2573219/1‑138 (MQ=255)

            cTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGTACGGCGCACAAATCGCGCTTAACGGGCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAAtt                                                                                                                          >  2:3450349/1‑140 (MQ=255)

            cTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAAtt                                                                                                                          >  2:1719049/1‑140 (MQ=255)

                  tGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCcgcg                                                                                                                    <  1:1171642/140‑1 (MQ=255)

                  tGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCcgcg                                                                                                                    <  1:3431007/140‑1 (MQ=255)

                   ggAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCgcgc                                                                                                                   >  2:622407/1‑140 (MQ=255)

                    gAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCt                                                                                                                  <  1:621627/140‑1 (MQ=255)

                    gAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCt                                                                                                                  <  1:4004758/140‑1 (MQ=255)

                       gTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCgg                                                                                                               >  1:1015161/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  2:4285947/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  2:1642559/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  1:1344063/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  2:5379892/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGATAATTTCCGCGCTCGGCa                                                                                                             >  2:4127585/1‑140 (MQ=255)

                           cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           >  2:497630/1‑140 (MQ=255)

                                     cGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTGGGCAAGAAAGATCATg                                                                                                 <  1:1089834/140‑1 (MQ=255)

                                     cGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATg                                                                                                 <  2:1128072/140‑1 (MQ=255)

                                       tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGcc                                                                                               >  2:4152505/1‑140 (MQ=255)

                                          tGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTg                                                                                            <  2:2276126/140‑1 (MQ=255)

                                           gcagcaGCTTGATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCGCGGCAAGAAAGATCATGCCTTGc                                                                                           <  1:3678401/140‑1 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTACCTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  1:5446127/1‑140 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  1:1842871/1‑140 (MQ=255)

                                             agcagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTg                                                                                         <  2:4233812/140‑1 (MQ=255)

                                               cagcTTTATCCTCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGGTTGTAGGCTGTAACTGCGGGATCATGGAGCAGCTAATTTCAGCGCTCGGCCAGAAAGCTCATGCCTTGCTGAt                                                                                       >  1:2343500/1‑140 (MQ=255)

                                               cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  2:3784588/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:5568236/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:4197348/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:4414006/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:4646529/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:794882/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:91492/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:2933360/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCATAACGGTCAGGAAGCAGAAAACGAGCGGGTAGTCTGTAAATGCGGGATCAGGGAACGGCTCATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGGTCg                                                                                     >  2:3474300/1‑140 (MQ=255)

                                                     tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  1:3450525/140‑1 (MQ=255)

                                                         atcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGc                                                                             <  1:4292024/140‑1 (MQ=255)

                                                         atcTGCAGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGCAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCCGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGc                                                                             <  2:2227358/140‑1 (MQ=255)

                                                            tggcGCTGGACGGAGCACAAATCGCGCTTAACGGGCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACGGCGGGATCATTGATCAGCTAATGGCCGCGCTCGGGGAGGAAGATCATGCCTTGCTGCTCGGTTGCCGCtga                                                                          >  2:5076136/4‑138 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  2:3892975/1‑140 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  2:3320046/1‑140 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGGTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCGGATCGATTGCCGCTCa                                                                          >  2:2601361/1‑140 (MQ=255)

                                                             gCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCAc                                                                         >  1:1297365/1‑140 (MQ=255)

                                                              ccGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACt                                                                        >  2:3145791/1‑140 (MQ=255)

                                                               cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  1:512594/140‑1 (MQ=255)

                                                                 cTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTggg                                                                     <  2:2704006/140‑1 (MQ=255)

                                                                         cgcACAAATCGCGCTTAGCGGTCAGGATGCAGACAGCCAGTTTGTAGGCTGTAGCTGCGGGATCATGGACCAGCTAATTGCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:2413803/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:5591437/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:4708745/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:3371237/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:4819391/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:4548761/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:1235110/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCCCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCCAAg                                                             >  2:3242934/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGCATGATGGAACAGCTGATTTCCGCGCGCGGCAAGAAAGAGCATGCCTTGCTGAGCGATTGCCGCTCGCTGGGGACGAAAg                                                             >  2:838958/1‑140 (MQ=255)

                                                                           cacaAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCa                                                           <  2:2020976/140‑1 (MQ=255)

                                                                           cacaAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTATAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTACCGCTGACTGGGGACCAAAGCa                                                           >  1:3108113/1‑140 (MQ=255)

                                                                            acaAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAg                                                          <  1:3016449/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:1586726/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:3276826/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:2491954/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:5201270/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGAATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:243005/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGATCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAACCAGTTTcc                                                     <  2:3952866/140‑1 (MQ=255)

                                                                                   gcgcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCAt                                                   >  2:1879154/1‑140 (MQ=255)

                                                                                   gcgcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCAt                                                   >  2:4028344/1‑140 (MQ=255)

                                                                                       ttACCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGccc                                               <  1:739585/140‑1 (MQ=255)

                                                                                        tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              >  1:5410418/1‑140 (MQ=255)

                                                                                        tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              >  2:5269667/1‑140 (MQ=255)

                                                                                            ggTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAgg                                          <  1:1465009/140‑1 (MQ=255)

                                                                                                    aGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTg                                  <  1:691896/140‑1 (MQ=255)

                                                                                                      cAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtc                                >  1:3832116/1‑140 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:2095734/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:4085488/140‑1 (MQ=255)

                                                                                                        gAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgt                              >  1:1965964/1‑140 (MQ=255)

                                                                                                         aaaaCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgtc                             <  2:4325300/140‑1 (MQ=255)

                                                                                                               aGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatcatc                       <  2:3033890/140‑1 (MQ=255)

                                                                                                                 tttGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCaa                     >  1:2965933/1‑140 (MQ=255)

                                                                                                                 tttGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCaa                     >  1:4729315/1‑140 (MQ=255)

                                                                                                                   tGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACa                   <  2:308650/140‑1 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  1:5578968/140‑1 (MQ=255)

                                                                                                                        gCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGTGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAAc              >  1:2756396/1‑140 (MQ=255)

                                                                                                                           gTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAATAGCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAATGGTGTGGCTGTCGTCATCATCAACAGTAACtgc           >  2:4438639/1‑138 (MQ=255)

                                                                                                                            tAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCAt                                                   >  2:4755775/1‑99 (MQ=255)

                                                                                                                            tAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCa          >  1:2030640/1‑140 (MQ=255)

                                                                                                                             aaCTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCaa         <  1:5404883/140‑1 (MQ=255)

                                                                                                                             aaCTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCaa         <  2:44870/140‑1 (MQ=255)

                                                                                                                               cTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAAc       <  2:2793702/140‑1 (MQ=255)

                                                                                                                                tGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACg      <  1:2285485/140‑1 (MQ=255)

                                                                                                                                tGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACg      <  1:2214236/140‑1 (MQ=255)

                                                                                                                                 gCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGt     >  2:3676267/1‑140 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  1:1007599/1‑140 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          >  2:1304655/1‑36 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  1:252329/140‑1 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  >  2:1390051/1‑140 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGCTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  >  2:2539417/1‑140 (MQ=255)

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`