BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,752,014	A→G	287 (TAA→TAG) K3E (AAG→GAG)	sgbU → araD →	L‑ribulose‑5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,752,014	0	A	G	99.6%	839.1 / NA	258	287 (TAA→TAG) K3E (AAG→GAG)	sgbU araD	L‑ribulose‑5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (156/100); minor base T (0/1); total (157/101)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

ACGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTA  >  NZ_CP025268/3751877‑3752150

aCGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTagag                                                                                                                                        <  2:1061297/140‑1 (MQ=255)

   cTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGGCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCaa                                                                                                                                     >  1:4100616/1‑140 (MQ=255)

    tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  1:4070269/1‑140 (MQ=255)

    tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  1:3920154/1‑140 (MQ=255)

      cATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTg                                                                                                                                  >  1:369713/1‑140 (MQ=255)

          aaCTGAACTAGCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCACAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAg                                                                                                                              <  2:2641417/140‑1 (MQ=255)

              gAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGa                                                                                                                          >  2:1777544/1‑140 (MQ=255)

              gAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGa                                                                                                                          <  2:1482404/140‑1 (MQ=255)

                aCTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACg                                                                                                                        >  1:2005613/1‑140 (MQ=255)

                aCTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACg                                                                                                                        >  1:3153829/1‑140 (MQ=255)

                  tATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTg                                                                                                                      >  2:3879934/1‑140 (MQ=255)

                    tCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGGAGCTCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCt                                                                                                                    >  1:3983348/1‑140 (MQ=255)

                    tCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCt                                                                                                                    >  2:5560241/1‑140 (MQ=255)

                    tCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCt                                                                                                                    >  1:1524579/1‑140 (MQ=255)

                     cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGGGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTg                                                                                                                   >  2:842711/1‑140 (MQ=255)

                     cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTg                                                                                                                   >  2:4329068/1‑140 (MQ=255)

                         gTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                               >  1:2842271/1‑140 (MQ=255)

                         gTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                               >  1:2327672/1‑140 (MQ=255)

                              ttCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAAt                                                                                                          >  2:5567074/1‑140 (MQ=255)

                              ttCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAAt                                                                                                          <  2:3739547/140‑1 (MQ=255)

                                    aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  1:5213804/1‑140 (MQ=255)

                                    aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  2:2965687/1‑140 (MQ=255)

                                    aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  1:5399106/1‑140 (MQ=255)

                                    aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  1:2681141/1‑140 (MQ=255)

                                    aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  2:504050/1‑140 (MQ=255)

                                    aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCGGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  2:1387785/1‑140 (MQ=255)

                                        agaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                                >  2:3586784/1‑140 (MQ=255)

                                        agaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                                >  2:1947293/1‑140 (MQ=255)

                                         gaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                               >  2:3621934/1‑140 (MQ=255)

                                              ggACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTc                                                                                          >  2:4351185/1‑140 (MQ=255)

                                               gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         <  1:588394/140‑1 (MQ=255)

                                               gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         <  1:772104/140‑1 (MQ=255)

                                               gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         >  2:2834749/1‑140 (MQ=255)

                                                    aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTGAGGCGCGGCGGTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCGCACCATc                                                                                    >  2:2028342/1‑140 (MQ=255)

                                                    aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    <  1:3833049/140‑1 (MQ=255)

                                                    aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTAGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  1:4772490/1‑140 (MQ=255)

                                                          ccAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGa                                                                              >  2:2427001/1‑140 (MQ=255)

                                                            aaaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACg                                                                            <  1:1576289/140‑1 (MQ=255)

                                                            aaaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACg                                                                            <  1:4852070/140‑1 (MQ=255)

                                                             aaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGt                                                                           <  2:1375895/140‑1 (MQ=255)

                                                             aaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGt                                                                           <  1:3470030/140‑1 (MQ=255)

                                                             aaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGt                                                                           <  2:369713/140‑1 (MQ=255)

                                                              agagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGtt                                                                          <  2:4761469/140‑1 (MQ=255)

                                                                  ccGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCAcc                                                                      <  2:1511917/140‑1 (MQ=255)

                                                                  ccGGTGCTGGAGATTATTCACGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATTTGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCTCTTCACGCTCACCATCTGGTGACGTTcccc                                                                      >  2:794097/1‑140 (MQ=255)

                                                                   cGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     <  1:4557974/140‑1 (MQ=255)

                                                                   cGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     <  2:4124651/140‑1 (MQ=255)

                                                                   cGGTGCTGGAGATTATTCAGGCGCGGCGGTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     <  2:3810293/140‑1 (MQ=255)

                                                                    ggTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTg                                                                    >  2:2876126/1‑140 (MQ=255)

                                                                     gTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTCTGCAGGAGGCTGGATTTATATGTTAGCGCAACGGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTgg                                                                   <  2:3449133/140‑1 (MQ=255)

                                                                     gTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCGGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTgg                                                                   >  2:1915531/1‑140 (MQ=255)

                                                                       gCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTgggg                                                                 <  1:2815486/140‑1 (MQ=255)

                                                                        cTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGTATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTTGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGc                                                                <  2:1246384/140‑1 (MQ=255)

                                                                        cTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGc                                                                <  1:3398801/140‑1 (MQ=255)

                                                                          ggAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCaa                                                              >  1:2736142/1‑140 (MQ=255)

                                                                                tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  2:4617318/1‑140 (MQ=255)

                                                                                tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  1:3535248/1‑140 (MQ=255)

                                                                                  ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGCGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      >  2:3086578/1‑140 (MQ=255)

                                                                                  ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      >  2:2577868/1‑140 (MQ=255)

                                                                                  ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      >  1:3393856/1‑140 (MQ=255)

                                                                                  ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCACACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      <  1:2102054/140‑1 (MQ=255)

                                                                                   tCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGCGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                     <  2:1563089/140‑1 (MQ=255)

                                                                                   tCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                     >  2:4925383/1‑140 (MQ=255)

                                                                                    cAGGCGCGGCGTTGGATTGAAGCGCCTATGCAGGAGGCTGGATATATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGcgcg                                                    <  1:5094631/140‑1 (MQ=255)

                                                                                     aGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTAGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAACGTCAGCGCgg                                                   >  2:4954469/1‑140 (MQ=255)

                                                                                       gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  2:5509428/1‑140 (MQ=255)

                                                                                          cggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCTGACGTGCTGACGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGAc                                              >  2:5146226/1‑140 (MQ=255)

                                                                                            gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGa                                            >  1:1198907/1‑140 (MQ=255)

                                                                                            gcgTTGGATTCAAGCGCGTATGCAGGATGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCGCCATCTGGTGACGTTCGCCTGGTGCAATGTCAGCGCGGTAGACgc                                            >  2:1112260/1‑139 (MQ=255)

                                                                                                     tGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                   <  1:1329367/140‑1 (MQ=255)

                                                                                                     tGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCGCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                   <  1:2967840/140‑1 (MQ=255)

                                                                                                      gAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCaa                                  <  1:5394124/140‑1 (MQ=255)

                                                                                                       aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  1:1825455/1‑140 (MQ=255)

                                                                                                       aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  2:1803304/1‑140 (MQ=255)

                                                                                                       aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  1:473734/1‑140 (MQ=255)

                                                                                                           gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  2:2885089/140‑1 (MQ=255)

                                                                                                           gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  1:1734599/140‑1 (MQ=255)

                                                                                                           gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  2:4560502/140‑1 (MQ=255)

                                                                                                            cgTATGCAGGAGGCTGGCTTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGTCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  1:1004477/1‑140 (MQ=255)

                                                                                                            cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     >  2:1226859/1‑99 (MQ=255)

                                                                                                            cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     <  1:1227013/99‑1 (MQ=255)

                                                                                                            cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  2:3357315/1‑140 (MQ=255)

                                                                                                            cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  1:5143872/1‑140 (MQ=255)

                                                                                                            cgTATGCAGGAGGCTGGATTTAGATGTCAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCGGGGGCAATGTCAGCGCGGCAGACGAAACGCGGCAatggagg                            >  1:611431/1‑138 (MQ=255)

                                                                                                                  cAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATc                      >  1:4072309/1‑140 (MQ=255)

                                                                                                                    ggaggCTGGATTTATCTGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGGTTCCCGCTCACCATCTGGCGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCaa                    >  2:4026001/1‑140 (MQ=255)

                                                                                                                     gaggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCaaa                   >  1:4204624/1‑140 (MQ=255)

                                                                                                                     gaggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGCTAATCaaa                   >  2:4088019/1‑140 (MQ=255)

                                                                                                                      aggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAAc                  >  2:1341576/1‑140 (MQ=255)

                                                                                                                       ggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAAcc                 >  2:2177138/1‑140 (MQ=255)

                                                                                                                        gCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         <  1:2927092/67‑1 (MQ=255)

                                                                                                                         cTGGATCTATATGTTAGAGCCACTGACAGCCGACGTACTGGCGGCACATCTGGCGCTTCCCTCTCACCATCTGACGACGTTCAACTGGGGGAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATCGTAATCAAACCtt               >  2:355419/1‑140 (MQ=255)

                                                                                                                          tGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTc              >  2:2377472/1‑140 (MQ=255)

                                                                                                                          tGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTc              >  1:1112980/1‑140 (MQ=255)

                                                                                                                               ttATAGGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCGGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCg         <  2:611431/140‑1 (MQ=255)

                                                                                                                                tataTGTTATAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTCGGGCAATGTCAGCCCGGTAGACGAAACGCGGCAATGGATGGTAATCCACCCTTCCGGCGt        <  2:2463157/140‑1 (MQ=255)

                                                                                                                                tataTGTTAGAGCAACTGAGAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGt        <  2:3183627/140‑1 (MQ=255)

                                                                                                                                 atatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGTGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc       >  1:4967808/1‑140 (MQ=255)

                                                                                                                                  tatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCg      >  1:3883460/1‑140 (MQ=255)

                                                                                                                                      ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTa  >  1:4175819/1‑140 (MQ=255)

ACGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTA  >  NZ_CP025268/3751877‑3752150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`