BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,971,997	A→G	pseudogene (1048/1048 nt)	wzzE →	ECA polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,971,997	0	A	G	100.0%	1166.6 / NA	350	pseudogene (1048/1048 nt)	wzzE	ECA polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (193/157); total (193/157)

Only 100 of 368 total aligned reads displayed.

CCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCT  >  NZ_CP025268/3971860‑3972133

ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATagcaa                                                                                                                                       <  2:3651133/140‑1 (MQ=255)

ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATagcaa                                                                                                                                       <  1:4659767/140‑1 (MQ=255)

ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATagcaa                                                                                                                                       <  1:4308906/140‑1 (MQ=255)

ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATagcaa                                                                                                                                       <  1:4173858/140‑1 (MQ=255)

  tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAgcaaca                                                                                                                                     <  1:4415479/140‑1 (MQ=255)

   aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTTTCGCATTAACCCTCCGTTGCTCGAAATAGCAacac                                                                                                                                    >  1:3050222/1‑140 (MQ=255)

   aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAacac                                                                                                                                    >  2:1988029/1‑140 (MQ=255)

   aTCGCTATTTGCGTACGCAGGAAGAACCGGTTAAAAGCCATAGCACACGTCGTGCGTTCCTGATGATTATGTGGGGCATCGTCGGGGGGG‑TGGTCGGGGCTGGTGGCGCAGGAACCCGCCGCTGCTGGAAATAgcagcag                                                                                                                                    >  2:5181575/1‑139 (MQ=255)

    tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACAct                                                                                                                                   <  1:3190548/140‑1 (MQ=255)

         aTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGGGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACtgctgc                                                                                                                              >  1:4231499/1‑140 (MQ=255)

          tttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCg                                                                                                                             >  2:5389672/1‑140 (MQ=255)

            tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGt                                                                                                                           >  2:2207254/1‑140 (MQ=255)

            tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGt                                                                                                                           <  1:2504349/140‑1 (MQ=255)

            tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGt                                                                                                                           <  2:670569/140‑1 (MQ=255)

             gCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTg                                                                                                                          >  1:1766652/1‑140 (MQ=255)

               gTACGCCGGAAGATCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAg                                                                                                                        <  2:4862021/140‑1 (MQ=255)

                  cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcgc                                                                                                                     >  1:1968329/1‑140 (MQ=255)

                           aaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCAGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                            <  1:3135486/140‑1 (MQ=255)

                             ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcg                                                                                                          >  2:1779799/1‑140 (MQ=255)

                                gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                       <  1:2340040/140‑1 (MQ=255)

                                  aacaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                                     <  2:2853619/137‑1 (MQ=255)

                                  aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                                     <  1:3750236/140‑1 (MQ=255)

                                          aTAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                             <  2:2704696/140‑1 (MQ=255)

                                              cccACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAgaga                                                                                         <  2:4356259/140‑1 (MQ=255)

                                                   gtcgtGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                                                                                    >  2:2575520/1‑140 (MQ=255)

                                                       tGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                                                                                >  1:4837215/1‑140 (MQ=255)

                                                        gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCACCACGGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                               >  1:3981452/1‑140 (MQ=255)

                                                        gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                               >  2:1072523/1‑140 (MQ=255)

                                                        gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                               >  2:2940016/1‑140 (MQ=255)

                                                        gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                               >  2:5224479/1‑140 (MQ=255)

                                                        gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                               <  1:1687702/140‑1 (MQ=255)

                                                        gCCTTCCTGATGATCATGGGGGGCATTGTC‑GGGGGCCTGATCGGGGCTGGTGTCGCGTTAACCCGCCGTTGGTCGAAATAGCAACACGGCTGCGGTGAGCGGAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                               >  1:796550/1‑140 (MQ=255)

                                                        ccccaccTGATGATTATGTGGAGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCAATGTGa                                                                               <  2:559795/135‑1 (MQ=255)

                                                            tCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGaa                                                                              >  1:724484/1‑137 (MQ=255)

                                                             ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                          <  1:476289/140‑1 (MQ=255)

                                                             ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                          >  1:506393/1‑140 (MQ=255)

                                                             ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                          <  2:4549136/140‑1 (MQ=255)

                                                               tgatgaTTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact                                                                        <  1:4848881/140‑1 (MQ=255)

                                                                gatgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                                       <  1:2837866/140‑1 (MQ=255)

                                                                gatgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                                       <  1:4546181/140‑1 (MQ=255)

                                                                gatgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                                       <  1:309659/140‑1 (MQ=255)

                                                                gatgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                                       <  2:5435779/140‑1 (MQ=255)

                                                                 atgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgg                                                                      >  2:1301729/1‑139 (MQ=255)

                                                                 atgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                                      >  1:3476283/1‑140 (MQ=255)

                                                                 atgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                                      <  2:5065249/140‑1 (MQ=255)

                                                                 atgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                                      <  2:4025974/140‑1 (MQ=255)

                                                                 atgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGGCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCGGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                                      <  1:4985644/140‑1 (MQ=255)

                                                                 atgatTATGTGGGGAATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACAGTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGATAATCGATGTGAAAGTactga                                                                      <  2:4696269/140‑1 (MQ=255)

                                                                  tgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                                     <  1:1521914/140‑1 (MQ=255)

                                                                  tgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                                     <  2:4924050/140‑1 (MQ=255)

                                                                   gatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact                                                                    <  1:2197645/140‑1 (MQ=255)

                                                                      tATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa                                                                 >  1:2667047/1‑140 (MQ=255)

                                                                        tgtggggCATTGTC‑GTGGGGCTGATCGGGGCTGGTGTCGAATTAACCCGCCGTTGCGCGAAATAGCAACACGGCTGTGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCTATGTGAAAGTATTTACTGTAtt                                                               >  2:2116723/4‑140 (MQ=255)

                                                                        tgtggggCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTAtt                                                               >  2:1269109/4‑140 (MQ=255)

                                                                           ggggCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTgg                                                            >  1:2407501/1‑140 (MQ=255)

                                                                             ggCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                                          >  1:5571258/1‑140 (MQ=255)

                                                                             ggCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                                          <  1:3941380/140‑1 (MQ=255)

                                                                             ggCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                                          >  2:4741085/1‑140 (MQ=255)

                                                                             ggCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                                          >  2:5002368/1‑140 (MQ=255)

                                                                              gCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAc                                                         <  1:3273458/140‑1 (MQ=255)

                                                                               cATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg                                                        >  1:61606/1‑140 (MQ=255)

                                                                               cATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg                                                        <  1:4012231/140‑1 (MQ=255)

                                                                               cATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg                                                        <  1:2567442/140‑1 (MQ=255)

                                                                               cATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg                                                        <  1:291998/140‑1 (MQ=255)

                                                                               cATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACCGTATTTGGTAcg                                                        <  2:5538719/140‑1 (MQ=255)

                                                                                   tgtcggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACgcgc                                                     <  2:1581842/136‑1 (MQ=255)

                                                                                   tggcggggggCTGATCGGGGCTGGTGTCTCATTAACCCGCCGTTGCTCGAAATAGGAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAGTCGAAGAGAGTCGATGTGAAAGTACTTACTGGATTTGGTACgcgc                                                     >  2:3232427/5‑140 (MQ=255)

                                                                                    gtcggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGcc                                                    <  2:2441406/137‑1 (MQ=255)

                                                                                       ggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCgg                                                 <  1:831229/140‑1 (MQ=255)

                                                                                        gggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa                                                <  1:5498164/140‑1 (MQ=255)

                                                                                         ggggCTGCTCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGCGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                               <  1:2059745/140‑1 (MQ=255)

                                                                                         ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                               <  2:1159697/140‑1 (MQ=255)

                                                                                         ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                               <  2:4479401/140‑1 (MQ=255)

                                                                                         ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGAGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                               <  1:215424/140‑1 (MQ=255)

                                                                                          gggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAg                                              >  1:5345895/1‑140 (MQ=255)

                                                                                            gCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGcc                                            >  2:4189257/1‑140 (MQ=255)

                                                                                             cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTCTTTGGTACGCGCCCGGAAGCCa                                           >  2:3103562/1‑140 (MQ=255)

                                                                                             cTGATCGGCGCTGGTTTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGGGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCa                                           >  2:3366453/1‑140 (MQ=255)

                                                                                               gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATc                                         >  1:2757037/1‑140 (MQ=255)

                                                                                               gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATc                                         >  1:2248011/1‑140 (MQ=255)

                                                                                               gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATc                                         >  2:746487/1‑140 (MQ=255)

                                                                                               gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATc                                         >  1:4144034/1‑140 (MQ=255)

                                                                                               gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATc                                         >  1:5180168/1‑140 (MQ=255)

                                                                                                      gCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg                                  <  1:3603878/140‑1 (MQ=255)

                                                                                                        tGGTGTCGCATTAACCCGCCGTCGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcg                                <  2:2395893/140‑1 (MQ=255)

                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAGTAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAGGGGGAAGTACTGACTGTATTTGGGACGCGCCCGGAAGCCGTCAAGGTGGCGcc                              >  2:1918581/1‑140 (MQ=255)

                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGcc                              >  2:4814422/1‑140 (MQ=255)

                                                                                                            gtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt                            <  2:4144034/140‑1 (MQ=255)

                                                                                                            gtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt                            <  2:1709216/140‑1 (MQ=255)

                                                                                                               gCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTgg                         >  2:2640386/1‑140 (MQ=255)

                                                                                                               gCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTgg                         >  2:2310405/1‑140 (MQ=255)

                                                                                                                    aaCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGCTGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCAt                    >  1:1278645/1‑140 (MQ=255)

                                                                                                                    aaCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCAt                    >  2:3627388/1‑140 (MQ=255)

                                                                                                                     aCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATg                   >  1:1012275/1‑140 (MQ=255)

                                                                                                                       ccgccgTCGCTCGAAATAGCAACACTGCTCCGGTGCGCGCAAAGGCGCTCGCCGCTTATTCGACGAGCATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGCAGCCATCAAGATGGCGCCGTTCGTGCATGCg                 <  2:1355313/140‑1 (MQ=255)

                                                                                                                          ccgTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCTTTg              >  1:1838447/1‑140 (MQ=255)

                                                                                                                          ccgTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAACGATGTGAAAGTACTGACTGTATTTGATACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCACGCGTTg              >  2:875959/1‑140 (MQ=255)

                                                                                                                           cgTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTgg             >  2:1420775/1‑140 (MQ=255)

                                                                                                                                  cGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGa      <  2:3811849/140‑1 (MQ=255)

                                                                                                                                     aaTAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATcc   >  2:2480846/1‑140 (MQ=255)

CCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCT  >  NZ_CP025268/3971860‑3972133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`