Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,476,316 A→G pseudogene (259/585 nt) bdcR → TetR/AcrR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,476,3160AG99.5% 1252.0 / NA 375pseudogene (259/585 nt)bdcRTetR/AcrR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (176/197);  minor base T (1/1);  total (177/198)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

TATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCA  >  NZ_CP025268/4476181‑4476452
                                                                                                                                       |                                                                                                                                        
tATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGa                                                                                                                                      <  1:4732036/140‑1 (MQ=255)
 aTCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAg                                                                                                                                     >  2:4547937/1‑140 (MQ=255)
  tCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTCGGCGAGt                                                                                                                                    >  1:2519531/1‑140 (MQ=255)
   cTTGGTATTAACCCCCGGCGCCGATACGCGGCTTATGGCAGTAAAGCTGGGTTATGTAGCCGTGTACTCAATGAATACGCCGGTACGCAAGCGATTCCGCTTGCCGAGATTCTTCGTGATGATCGTCCGGTAGGCGAGTg                                                                                                                                   <  2:4130360/140‑1 (MQ=255)
    ttGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGc                                                                                                                                  <  2:2304514/140‑1 (MQ=255)
        tATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTgg                                                                                                                              >  2:3673894/1‑140 (MQ=255)
           tAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTg                                                                                                                           <  2:3276167/140‑1 (MQ=255)
            aaCCCCCCGCGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTCGCCGTGTACTCAATCAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGa                                                                                                                          <  1:2935439/140‑1 (MQ=255)
            aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGATGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGTTTGa                                                                                                                          >  1:3070813/1‑140 (MQ=255)
             aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAg                                                                                                                         <  2:5489431/140‑1 (MQ=255)
             aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAg                                                                                                                         >  1:1324519/1‑140 (MQ=255)
                  ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:467877/1‑140 (MQ=255)
                  ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  2:2965757/1‑140 (MQ=255)
                  ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  2:2513281/1‑140 (MQ=255)
                  ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:1282433/1‑140 (MQ=255)
                  ccGAGCCTCTACGCGGCATTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTGGAGGTAtt                                                                                                                    >  2:3688935/1‑140 (MQ=255)
                         tctACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAaagaa                                                                                                             >  2:1314723/1‑140 (MQ=255)
                         tctACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAaagaa                                                                                                             >  1:3271534/1‑140 (MQ=255)
                           tACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAgc                                                                                                           >  1:1947523/1‑140 (MQ=255)
                             cgcgGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcgg                                                                                                         <  1:3464617/140‑1 (MQ=255)
                                 gCTTTTGGCAGTAAAGGTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     <  1:2343650/140‑1 (MQ=255)
                                 gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     <  1:939351/140‑1 (MQ=255)
                                 gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     <  1:2811440/140‑1 (MQ=255)
                                 gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     <  1:4233152/140‑1 (MQ=255)
                                      tGGCAGTAAAGCTGGTTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAGGCTATTCCGCTTGCCGATATTCTTCGTGATAATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGat                                                                                                <  2:2812553/140‑1 (MQ=255)
                                            tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGcc                                                                                          >  1:4912909/1‑140 (MQ=255)
                                            tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGcc                                                                                          >  1:2025627/1‑140 (MQ=255)
                                            tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGcc                                                                                          >  1:1791063/1‑140 (MQ=255)
                                            gaaaGCTGGGTTATTTAGCCGTGGACGCAAAGAATACGTCGGTACGGAAGCCATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTCGGCGAGAGCCTGGCTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGcc                                                                                          >  1:3359560/2‑140 (MQ=255)
                                                    ggTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAAcggc                                                                                  >  2:4322750/1‑140 (MQ=255)
                                                      gtagttAGCGGTGTACTCAATGAATACGGTGGTACGTCAGCTGTTCCGTTTGCCGATCTTCTTCGTGATGATGGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAACGTAGCGGCGCGCAGATATAGCGAAGAcggcgg                                                                                >  2:5319448/5‑140 (MQ=255)
                                                            aGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                          <  1:3978750/140‑1 (MQ=255)
                                                            aGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                          <  1:4078865/140‑1 (MQ=255)
                                                             gCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgct                                                                         <  2:1218880/140‑1 (MQ=255)
                                                              ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctg                                                                        >  1:1622918/1‑140 (MQ=255)
                                                              ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctg                                                                        >  1:4852775/1‑140 (MQ=255)
                                                               cGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTACAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctgg                                                                       <  2:4809596/140‑1 (MQ=255)
                                                                   tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGt                                                                   >  1:5463331/1‑140 (MQ=255)
                                                                   tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGt                                                                   >  2:183772/1‑140 (MQ=255)
                                                                   tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGt                                                                   >  1:382007/1‑140 (MQ=255)
                                                                   tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGt                                                                   >  2:528317/1‑140 (MQ=255)
                                                                     cTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTAt                                                                 <  1:3015231/140‑1 (MQ=255)
                                                                     cTCAATGAATACGTCGGTAAGGAAGCTATTCCTCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTAt                                                                 <  2:2053611/140‑1 (MQ=255)
                                                                        aaTGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGt                                                              >  1:602549/1‑140 (MQ=255)
                                                                        aaTGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGt                                                              >  1:4961157/1‑140 (MQ=255)
                                                                        aaTGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGt                                                              >  2:1417103/1‑140 (MQ=255)
                                                                             aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCATAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                         <  2:4264929/140‑1 (MQ=255)
                                                                             aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                         >  1:732088/1‑140 (MQ=255)
                                                                             aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                         >  2:778886/1‑140 (MQ=255)
                                                                             aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                         <  1:1024255/140‑1 (MQ=255)
                                                                               aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                                       <  1:5210642/140‑1 (MQ=255)
                                                                               aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                                       <  2:5130403/140‑1 (MQ=255)
                                                                               aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                                       <  2:5547915/140‑1 (MQ=255)
                                                                               aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                                       <  1:4636525/140‑1 (MQ=255)
                                                                                cGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAg                                                      <  2:2409016/140‑1 (MQ=255)
                                                                                cGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAg                                                      >  1:1055473/1‑140 (MQ=255)
                                                                                cGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAg                                                      >  2:1971562/1‑140 (MQ=255)
                                                                                cGTCGGTACGGAAGCTATTCCGCTTGCAGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAg                                                      <  2:3061477/140‑1 (MQ=255)
                                                                                  tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGt                                                    >  1:870063/1‑140 (MQ=255)
                                                                                  tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGt                                                    <  1:1008181/140‑1 (MQ=255)
                                                                                  tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGt                                                    >  1:1739472/1‑140 (MQ=255)
                                                                                    ggTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTAt                                                  >  1:1917609/1‑140 (MQ=255)
                                                                                      tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTc                                                >  2:4972476/1‑140 (MQ=255)
                                                                                      tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTc                                                >  2:2113086/1‑140 (MQ=255)
                                                                                      tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTc                                                >  2:595679/1‑140 (MQ=255)
                                                                                       aCGTAAGTTATTCCGCTTTCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                               <  2:1343422/140‑1 (MQ=255)
                                                                                       aCGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                               <  2:3791353/140‑1 (MQ=255)
                                                                                       aCGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                               <  1:3208858/140‑1 (MQ=255)
                                                                                       aCGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                               <  2:3428589/140‑1 (MQ=255)
                                                                                       aCGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                               <  1:5001376/140‑1 (MQ=255)
                                                                                             gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:1788221/140‑1 (MQ=255)
                                                                                             gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:2189940/140‑1 (MQ=255)
                                                                                             gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:3634970/140‑1 (MQ=255)
                                                                                             gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:1735121/140‑1 (MQ=255)
                                                                                             gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:1659245/140‑1 (MQ=255)
                                                                                             gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:3376371/140‑1 (MQ=255)
                                                                                               tATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATg                                       <  1:2512918/140‑1 (MQ=255)
                                                                                                aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGa                                      >  1:1217532/1‑140 (MQ=255)
                                                                                                aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGGTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGa                                      >  2:3217653/1‑140 (MQ=255)
                                                                                                 ttCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGAt                                     <  2:1077871/140‑1 (MQ=255)
                                                                                                     gCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCcac                                 <  1:2562440/140‑1 (MQ=255)
                                                                                                      cgtGCCGATATTCTTCGTGATTATCGTCAGGTAGGCTAGTGCCTGGTTGAGGTATTAAAAGAAGCGTCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATAGTTCTTGAAGGTATTCATAGTCGTGATCcaca                                >  2:275201/3‑140 (MQ=255)
                                                                                                      cTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGa                                                                                                                          <  2:4449398/50‑1 (MQ=255)
                                                                                                      cTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGa                                                                                                                          >  1:4449398/1‑50 (MQ=255)
                                                                                                          ccGATATTCTTCGTGATTATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgcg                            <  2:5090258/140‑1 (MQ=255)
                                                                                                          ccGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgcg                            <  1:142132/140‑1 (MQ=255)
                                                                                                           cGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGCATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgcgc                           >  1:5134723/1‑140 (MQ=255)
                                                                                                            gATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGcgcg                          <  1:3403432/140‑1 (MQ=255)
                                                                                                                   ttcGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATAtt                   >  1:479734/1‑140 (MQ=255)
                                                                                                                    tcctgctgatCGTCCTGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGTCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATAttt                  <  2:391559/134‑2 (MQ=255)
                                                                                                                    tcGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTg                  <  2:2573302/140‑1 (MQ=255)
                                                                                                                     cGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     >  1:5544810/1‑56 (MQ=255)
                                                                                                                     cGTGAGGATCGTCCCGGAGGCGCGTGCCTGGTCGAGGTATTAAAAGGAGCGGCGGGCCGCTATAGCCAAAACGGCGGCTCCGCTGGCTGTACGGCTCTTGAAGGCATTAATAGTCAGGATCCACACGCGCGTGATATTGc                 >  2:822561/1‑140 (MQ=255)
                                                                                                                       tgatgaTCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCg               <  1:3676319/140‑1 (MQ=255)
                                                                                                                         atgatCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGtt             <  1:4130865/140‑1 (MQ=255)
                                                                                                                         atgatCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGtt             <  1:4547937/140‑1 (MQ=255)
                                                                                                                          tgatCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTc            <  1:2670835/140‑1 (MQ=255)
                                                                                                                             tCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCGACAAGCGCGTGATATTGCCGTTCAat         >  2:268727/1‑140 (MQ=255)
                                                                                                                                tCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAAtatt      >  1:2011201/1‑140 (MQ=255)
                                                                                                                                    gTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCa  <  2:5440250/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCA  >  NZ_CP025268/4476181‑4476452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑