BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	96.8%	791.1 / ‑0.6	251	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (5/3); new base A (123/118); total (129/122)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG  >  NZ_CP025268/4608020‑4608289

ccACGTCCGTAAGGACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                    <  1:445618/140‑1 (MQ=255)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                    <  1:3467452/140‑1 (MQ=255)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATCTTACTTTATTTGATATCCCCCCGTTTAGTGCGA‑GGGTGGGGGCTTGAACCCCCTCGTCAATAAGAACACt                                                                                                                                    >  2:4353229/1‑140 (MQ=21)

  aCGTCCGTAAGGACACTAACACCTGGAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                  <  1:1103783/140‑1 (MQ=255)

  aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                  >  1:4596045/1‑140 (MQ=255)

  aCGGCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                  <  2:5473336/140‑1 (MQ=255)

      ccGTTAGGACAATAACCCCTGCAGATAGTGCGTCTACCAATTCCGGCACCTTCGCACAGTCATCTTACTTTTTTTAATATAGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGATCCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                              <  1:1919998/140‑1 (MQ=25)

      ccGTAAGGACACTAACACCTGAAGCTAGCACGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                              <  2:2262196/140‑1 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCACAATACTTTTTTTGATATCCCCCCGCTGGGGGCGA‑GGGGGGTGACTTGAACCCCCACGTCCGTAATAACACTACCACCTg                                                                                                                            >  2:2791275/1‑140 (MQ=21)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                    <  1:3965798/140‑1 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTCTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGGTGGAACCCCCACGTCCGTAAGAACACTAACACCTCAAGCTaac                                                                                                                    >  1:5264010/1‑138 (MQ=37)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTATTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTAAAGCTCgc                                                                                                                    >  2:1510609/1‑140 (MQ=255)

                         tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTATCTGATATCCCCTCGTTTAGTGCGA‑GGGGAGGGACTTGAACCCCCACGTCCGTAAGAACACTGACACCTGAATCTACCGCGTCGAcc                                                                                                           >  2:4906775/1‑140 (MQ=25)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                          >  1:1492811/1‑140 (MQ=255)

                           aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCGTACTTTTTTTGATATCGCCGCGTTTGGTGCGA‑TGGCGGGGGCTTGAACCCCCACGTCCGTACGAACACTGAGACCTGAAGCTACCGCCTCTACCaa                                                                                                         >  2:2450452/1‑140 (MQ=25)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAGGCTAGTGCGTCTACCAATTcct                                                                                                    >  1:1512271/1‑139 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:4135088/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:3788089/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:4662327/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCAGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcgg                                                                                                    >  2:3310778/1‑138 (MQ=255)

                                 gcgcGTCTTCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                   <  2:2026794/140‑1 (MQ=255)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                   >  2:1333592/1‑140 (MQ=255)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                   <  1:1183088/140‑1 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCGTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCGTGAACCCCCACGTCCGTAAGGACGCTAACGCCGGAAGCTAGAGGGTGTAGAAATTCCggc                                                                                                  >  2:3454196/1‑138 (MQ=25)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGTTATCGTCTCGTTTGGTGCGA‑GGGCGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCACGTCTACCAATTCCgtc                                                                                                  >  1:474938/1‑138 (MQ=25)

                                         aCCAATTCCGCCACCTTCGCACAGCCTCATTACTTTTTTTGATATCCCCCCGTTTGGGGCCA‑TGGGGGGGGCTTTAAGCTCTACGTCCGTACGTAATTTACCACCGCAAGCTATCGCGTCTACAAATTCCCCCGCCGTca                                                                                           >  2:3327837/1‑139 (MQ=255)

                                           cAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  1:4348904/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  1:5156334/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  1:4411931/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:3152672/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTCACTCTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:4630294/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTAATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:3695972/140‑1 (MQ=255)

                                                ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                    <  1:3167859/140‑1 (MQ=255)

                                                ccGCCACCTACCCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                    <  2:780617/140‑1 (MQ=255)

                                                ccGCCAACTTCGCACAGTCACCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                    <  1:226456/140‑1 (MQ=255)

                                                 cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                   >  1:2319777/1‑140 (MQ=255)

                                                  gCCACCTTCGCACAGTCATCTTACTGTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                  <  1:3930309/140‑1 (MQ=255)

                                                    cACGTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  2:832690/140‑1 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  2:1567239/140‑1 (MQ=255)

                                                    cACCTTCGCACAATCATCTTACTTTTTTTGATGTCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  1:264995/140‑1 (MQ=255)

                                                    cACCTTCGATAAGTGATCTTACTTGTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  2:4990521/140‑1 (MQ=18)

                                                           gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                                         >  1:3309781/1‑140 (MQ=255)

                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                      <  1:886605/140‑1 (MQ=255)

                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                      <  1:3828414/140‑1 (MQ=255)

                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACCTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTACCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                      <  2:4430082/140‑1 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                   >  1:3711899/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                   >  1:183289/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAACAATAACaaagag                                                                   >  2:681797/1‑136 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCGACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                   >  1:3280545/1‑140 (MQ=255)

                                                                  cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                                                                  >  1:4248372/1‑140 (MQ=255)

                                                                      ttACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                                                              <  1:1000410/140‑1 (MQ=255)

                                                                      ttACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                                                              >  1:3900896/1‑140 (MQ=255)

                                                                        aCTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGtttttc                                                            >  2:2343143/1‑139 (MQ=255)

                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttcttcg                                                           >  2:4563045/1‑138 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTGTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                         >  1:3321935/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGtc                                                         >  2:563915/1‑139 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                         >  1:4894939/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                         <  2:4042171/140‑1 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCAA‑GGGTGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTTGTTTTTgttt                                                         >  2:2378021/1‑137 (MQ=21)

                                                                             ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTACGAGCAATAACAACGTGGTTTTTGGTGCg                                                       >  2:1031690/1‑140 (MQ=255)

                                                                              tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACATCCGGCAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTTCGa                                                      >  2:2603501/1‑140 (MQ=21)

                                                                               ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAg                                                     >  2:4848326/1‑140 (MQ=255)

                                                                                tGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg                                                    <  1:1687687/140‑1 (MQ=255)

                                                                                    atCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                >  2:4887215/1‑140 (MQ=255)

                                                                                    atCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                <  1:2420048/140‑1 (MQ=255)

                                                                                             tttGGTGCGAGGGGGGGGGACTTGACCCCCCCCGTCCGTAGGACCATTAACACCTGAAGCAAGCGGGTCTACCAATTCCGCCACCTTCGCCCAGTCCGACCAATAAAAAAGTGGTTTTTGGTGGGA‑GTGGGGGACTTgga                                       >  2:3853465/1‑138 (MQ=9)

                                                                                                 gTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                                   <  1:4545255/140‑1 (MQ=255)

                                                                                                  tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCCATGCGATCAATATCAACGAGGTTTTTGGTCCGAGGGGCGGGACTTGAccccgc                                  >  2:3560076/1‑138 (MQ=18)

                                                                                                  tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                  <  1:1008460/140‑1 (MQ=255)

                                                                                                  tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCAATATCAACGTGGTTTTTGTTGGGAGGGGGGTGCCTGGGAccctc                                  >  1:5377976/1‑138 (MQ=18)

                                                                                                   gcta‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  2:411464/137‑1 (MQ=255)

                                                                                                   gCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  1:2528765/140‑1 (MQ=255)

                                                                                                   gCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATCCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  1:93468/140‑1 (MQ=255)

                                                                                                    cGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCAc                                <  2:62559/140‑1 (MQ=255)

                                                                                                      a‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGt                              >  1:2806717/1‑140 (MQ=255)

                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc                             >  1:4928308/1‑140 (MQ=255)

                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGCTGCGAGGGGGGGGACTTGAACCCCCACGTc                             >  2:1805565/1‑140 (MQ=255)

                                                                                                            ggggACTTGAACCCCCATATCAGTAAGAACACAAACACCTGAAGATAGCGCGGCTACCAAGTCCGACACCCACGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                         <  2:2087503/140‑1 (MQ=25)

                                                                                                            ggggACCTGACCCCCCACGTCCGTACGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCCACGCCCAGTGCGAGCAATATCAACGGGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                         <  2:1599610/140‑1 (MQ=25)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTCCGAGGGGGGGGACTGGGACCCCCAGGTCCTTaa                        >  2:4506886/1‑140 (MQ=21)

                                                                                                              ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                       <  1:1839554/140‑1 (MQ=255)

                                                                                                              ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                       <  1:1127426/140‑1 (MQ=255)

                                                                                                              ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                       <  1:4695450/140‑1 (MQ=255)

                                                                                                              ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAACTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                       <  1:2061910/140‑1 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                                                    <  2:3828667/89‑1 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  1:1333592/140‑1 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTCGAACCCCCACGTCCGTAAGGACACt                 >  1:2219701/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGAGGGGCGGTACTTTAACCCCCACGTCCTTATGAACACt                 >  2:5165376/1‑140 (MQ=21)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACATGAAGCTAGCGCTTCTACCGATACCGCCACCTTAGCCCGGTGCGAGCAAGATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  2:2693613/140‑1 (MQ=37)

                                                                                                                      ccccccACGTCCGTAAGAACACTAACACCTCAAGCTAGTGCGACTACCAATCCCCCCCCCTTCGCCCAGTGCCAGCAATATCAACGTGATTTTTGGTGCGAGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTaa               <  1:2763418/139‑1 (MQ=25)

                                                                                                                      aCCCCCACGTCCTTAAGAACACTAACACCTGAAGCTAGCGCGGCGACCAAGTCCGCCACCCTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa               <  1:713875/140‑1 (MQ=37)

                                                                                                                          ccACGTACGTAAGAACACATACAGCTGAAGATAGCGCGTCTACCAATACCGCCACCCTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc           <  2:5555346/140‑1 (MQ=25)

                                                                                                                          ccACCTCCGTAAGAACCCTACCACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTAGCCCAGTGCGAGCAATATCAACGTCGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc           <  2:3496917/140‑1 (MQ=25)

                                                                                                                           cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTCACACCt          >  1:4713621/1‑140 (MQ=255)

                                                                                                                           cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTGGAGCCCCCACGTCCTTGAGAACAGTAACACCt          >  2:2554975/1‑140 (MQ=25)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACTTCCGTAAGGACACTAACACCTg         >  2:3110400/1‑140 (MQ=255)

                                                                                                                              gTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTCGTGCGAGGGGGGGTACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa       >  2:1877926/1‑140 (MQ=255)

                                                                                                                                  gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACATGAAGCTa   >  2:1517212/1‑140 (MQ=255)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg  >  1:1649474/1‑140 (MQ=255)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTCCGAGGGGGGGGATAGGAACCCCCACGTCCGTAACGATACTAACACCTGAAGCGAg  >  2:1457227/1‑140 (MQ=25)

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG  >  NZ_CP025268/4608020‑4608289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`