BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,621,820	G→A	G104E (GGA→GAA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,621,820	0	G	A	99.1%	1165.8 / NA	326	G104E (GGA→GAA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (149/173); total (153/173)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

TCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGT  >  NZ_CP025268/4621683‑4621956

tCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGaaa                                                                                                                                        >  1:5436349/1‑140 (MQ=255)

tCTGGGGCTGGCGAAAGCACACGAAGGTTCAACCGGTTTCATCCCGGCGGGAATGGACAGCAACCCTGAGGTTAGCGGCGCGTACGCATGGGCGCAAGAAATGTCATCCAGGAAAGATACCCCGTCTGGGCACTGGGaaa                                                                                                                                        >  2:1833419/1‑140 (MQ=255)

 cTGGGGCTGTTGAATGCACACGAAGGTTCGATCGGTGTCATTCAGGCGGCAATGGACGGCAACGCGGAAGTTATTGGCGCTTACGCATGGGCGCACGAAATGTCATCCGGTAAAGGTACCCCGTCTGGGTACTGGGGGAt                                                                                                                                       >  2:2687796/1‑140 (MQ=255)

 cTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAAt                                                                                                                                       >  1:5510476/1‑140 (MQ=255)

     ggCTGGCGAAAGCACAGGAAGGTTCTACCGGTTCCATTCCGGCGGGAAGGGACGGCCACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAAATAACCCGGCTGGTCACTGGGAAATTgac                                                                                                                                   >  2:5022341/1‑138 (MQ=255)

      gCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCg                                                                                                                                  <  2:1114464/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  1:1531661/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  1:473894/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  2:3742974/140‑1 (MQ=255)

         ggCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtg                                                                                                                               >  1:3378409/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGGTACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  2:5213671/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:5233350/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:952497/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:617797/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACCGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTTCCGgtgt                                                                                                                              >  2:123461/1‑140 (MQ=255)

               aGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCgg                                                                                                                         <  2:701087/140‑1 (MQ=255)

                gCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGt                                                                                                                        <  1:5381025/140‑1 (MQ=255)

                 cacaCGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGtt                                                                                                                       <  1:138833/140‑1 (MQ=255)

                       aaGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGttt                                                                                                                 <  1:3216339/140‑1 (MQ=255)

                          gTTCTACCGGTTTCATTACGGCGGGAATGGACGGCAAAGCAGAAGTTATTGGCGCGTATGCATGGGCGCACGAAAGGTCATCAGGTAAAGATACCCCGTTTGGTCACTGGGAAACTGACGGTGTCCGTGTTGCGTTTGAg                                                                                                              >  2:2492958/1‑140 (MQ=255)

                           ttCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                             <  1:1021000/140‑1 (MQ=255)

                           ttCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGGTCTGTTTGAGt                                                                                                             >  2:1027844/1‑140 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCTACGCTGATGTTATCGGCGCGTACGCATGGGCGCACGAAATCTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGCGTCCCGGTTCTGTTTGAGTgg                                                                                                           <  2:4648509/140‑1 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                           <  2:1563660/140‑1 (MQ=255)

                              tACCGGTTTCATTCCGGCTGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAGAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTggg                                                                                                          >  2:4893175/1‑140 (MQ=255)

                              tACCGGTTTCATTCCGACGGGAATCGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCCTCCGGCAAAGATCCCCCGTCTGGTCAATGGGAAATTGCCGGTGCGCAGATTCTGCTTCAGTggg                                                                                                          >  2:5289448/1‑140 (MQ=255)

                                ccGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGa                                                                                                        <  2:219074/140‑1 (MQ=255)

                                   gTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGatat                                                                                                     <  2:836289/140‑1 (MQ=255)

                                    tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAtt                                                                                                    >  1:2585697/1‑140 (MQ=255)

                                     ttCATTCCGGCGGGCATGCACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGGCACTGGGAAATTGCCGGTGTCCCGTCTCTGTTGGAGTGGGGATAttt                                                                                                   >  2:3149319/1‑140 (MQ=255)

                                     ttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAttt                                                                                                   >  1:4425881/1‑140 (MQ=255)

                                     ttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAttt                                                                                                   >  1:3044767/1‑140 (MQ=255)

                                          tCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                              <  2:3411069/140‑1 (MQ=255)

                                                   aaTGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGaaa                                                                                     >  2:1451417/1‑140 (MQ=255)

                                                       gACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAg                                                                                 <  1:2264774/140‑1 (MQ=255)

                                                         cGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCt                                                                               >  1:385673/1‑140 (MQ=255)

                                                         cGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCt                                                                               <  2:2964742/140‑1 (MQ=255)

                                                         cGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCt                                                                               <  1:1238166/140‑1 (MQ=255)

                                                               cGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGc                                                                         >  1:5175959/1‑140 (MQ=255)

                                                               cGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAAGGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGc                                                                         >  1:719573/1‑140 (MQ=255)

                                                                  tGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAag                                                                      <  1:1150328/140‑1 (MQ=255)

                                                                    aaGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAagag                                                                    <  1:2521743/140‑1 (MQ=255)

                                                                      gTTCTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTCGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                  <  2:5378665/140‑1 (MQ=255)

                                                                      gTTATCGGCGCGTACGCGTTGGCGCACGAAATGTCAGCCGGTAAAGATACCCCGTCTGGGCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                  <  2:3812290/140‑1 (MQ=255)

                                                                      gTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                  <  2:1680296/140‑1 (MQ=255)

                                                                      gTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                  <  2:744885/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  2:1916785/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:1088055/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:857397/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:2391038/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:3719686/140‑1 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGTTGTCCCGGTTCTGTTTGAGTGGGGATATTtct                                                                                                 >  2:1947534/1‑106 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTtct                                                                                                 <  1:1947364/106‑1 (MQ=255)

                                                                          tCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTGTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGctgctg                                                              >  1:175725/1‑140 (MQ=255)

                                                                              cgcgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                          <  1:5070127/140‑1 (MQ=255)

                                                                                cgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATaaa                                                        >  1:2060713/1‑140 (MQ=255)

                                                                                       aTGGGCGCACGAAATGTCATCCGGTACAGATACCCCGTCTGGTCACTGGGAAATTGACGGTGTCACGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCg                                                 <  2:3050231/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                <  2:3924289/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                <  2:452573/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                <  2:5485160/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                >  1:4209380/1‑140 (MQ=255)

                                                                                         gggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGaa                                               <  1:2822533/140‑1 (MQ=255)

                                                                                             gcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGTGGATCTTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAATCTGGTCGAAcgcg                                           <  2:4448159/140‑1 (MQ=255)

                                                                                              cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTTCCGGAATACAGACACTGCTGGAGCGCGGCTATTCCTGCCATCACGAAAACAGCTGCCCGCAAGAGCTGATGGACAAACTTGTCGacccata                                          >  2:4757065/1‑134 (MQ=255)

                                                                                              cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACgcgc                                          >  1:2789034/1‑140 (MQ=255)

                                                                                              cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATATCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACgcgc                                          >  1:4657113/1‑140 (MQ=255)

                                                                                              cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATATCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACgcgc                                          >  1:4658752/1‑140 (MQ=255)

                                                                                                cGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTa                                        <  2:2116666/140‑1 (MQ=255)

                                                                                                 gAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTaa                                       >  1:2182259/1‑140 (MQ=255)

                                                                                                    aTGTCAATCAGTAAAGATACCCCGTCTGATCACTGGTAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATATCTCCGATCACGATAACAGCTTCCCGCCAGAGCTGCTGGATAAACTCGTCGAACGCGCTAATCt                                    <  2:4336581/140‑1 (MQ=255)

                                                                                                     tGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTg                                   <  1:5424515/140‑1 (MQ=255)

                                                                                                     ggTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTg                                   <  2:2009007/139‑1 (MQ=255)

                                                                                                      gTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGc                                  <  2:3223663/140‑1 (MQ=255)

                                                                                                         aTCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCgg                               >  2:4290839/1‑140 (MQ=255)

                                                                                                          tCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCggg                              <  1:3461030/140‑1 (MQ=255)

                                                                                                           ccGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGt                             <  2:3942269/140‑1 (MQ=255)

                                                                                                           ccGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGt                             <  2:3545760/140‑1 (MQ=255)

                                                                                                             ggTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGGAAGAGCTGCTGGATAAACTGGGCGAACGCGCTAATCTGGCGGGTTa                           >  2:3425469/1‑140 (MQ=255)

                                                                                                              gTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTAc                          >  1:1830926/1‑140 (MQ=255)

                                                                                                              gTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTAc                          <  1:1858327/140‑1 (MQ=255)

                                                                                                               tAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTAcc                         >  1:4029006/1‑140 (MQ=255)

                                                                                                               tAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGGTAcc                         >  1:2861305/1‑140 (MQ=255)

                                                                                                               tAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTATGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTAcc                         <  2:1774499/140‑1 (MQ=255)

                                                                                                                    aTACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATACACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGt                    <  1:2292835/140‑1 (MQ=255)

                                                                                                                    aTACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGt                    <  2:2606132/140‑1 (MQ=255)

                                                                                                                    aTACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGt                    <  2:4968720/140‑1 (MQ=255)

                                                                                                                      aCCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTaa                  >  1:4266617/1‑140 (MQ=255)

                                                                                                                       ccccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAAc                 <  2:2795035/140‑1 (MQ=255)

                                                                                                                        cccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACt                >  1:2704259/1‑140 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            <  1:39325/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            <  2:483142/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            >  1:3620597/1‑140 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            >  1:1478713/1‑140 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAc           <  1:1982075/140‑1 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAc           >  2:2385873/1‑140 (MQ=255)

                                                                                                                              tGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAct          >  1:1698420/1‑140 (MQ=255)

                                                                                                                                     tGGGAATTTGCCGGTTCCCCGGTTCTGTTTGAGTGGGGATATCTCTCCGATCACTAAAACAGCTTCCCGCAAGAGCTGCTGTATACACTGTTCGAACGCCCTAATCTGCCCGGTTACCTCGGTAACTGCCACTCTTCCgg   <  2:3015243/140‑1 (MQ=255)

                                                                                                                                     tGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCgg   >  2:5578167/1‑140 (MQ=255)

                                                                                                                                     tGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCgg   >  2:896011/1‑140 (MQ=255)

                                                                                                                                     tGGGAAATTGCCGGTGTCCCGGTTCTGATTGAGTGGGGATATTTCTCCAATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGACAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCTAGTCTTCCgg   >  2:4096627/1‑140 (MQ=255)

TCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGT  >  NZ_CP025268/4621683‑4621956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`