| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP025268 | 390,887 | G→A | A356V (GCG→GTG) | CXP41_RS02035 ← | IS3‑like element IS3 family transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP025268 | 390,887 | 0 | G | A | 81.2% | 12.3 / ‑3.9 | 16 | A356V (GCG→GTG) | CXP41_RS02035 | IS3‑like element IS3 family transposase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (12/1); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACGCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCTCATACTTCCACG > NZ_CP025268/390751‑391014 | ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGTCTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 1:1129614/1‑140 (MQ=255)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 1:1888743/1‑140 (MQ=9)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 1:1953485/1‑140 (MQ=18)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 1:3538698/1‑140 (MQ=12)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 1:4181596/1‑140 (MQ=12)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 1:5341277/1‑140 (MQ=12)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 2:1111497/1‑140 (MQ=9)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 2:1112598/1‑140 (MQ=12)ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGCTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg > 2:1109759/1‑140 (MQ=255) ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt < 1:4263188/140‑1 (MQ=2) ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt > 2:1324427/1‑140 (MQ=0) ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTGCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt > 2:11203/1‑140 (MQ=9) ggCTGATTGATCATACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt > 2:5515345/1‑140 (MQ=0) gcctAAGCGAGGTTATTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACCCTAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACGCCGTTGCCCGCATTATTTCCCGGCTGCTAGAGTGTtcgtc > 2:4080567/4‑137 (MQ=11) agtcatacCAACTGTGCCGCCCCAACCGATTGTAATCACATTCGATATAATTAAACGCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTTTTCTCCATGGATTCATTCCACTTTCAGCATATGAATGAAGCTTTCCACg < 2:644016/134‑1 (MQ=11) agataATTAAAGGCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACAGTCCACTTTCAGCGAATGAAAGAAGCGTTCCACGCAGGCAGTATCGTAGCAGCAACCTTTTGCGCTCATACTTCCACg < 2:5345041/138‑1 (MQ=11) | GGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACGCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCTCATACTTCCACG > NZ_CP025268/390751‑391014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |