BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	474,717	C→T	P97S (CCA→TCA)	ybaY →	YbaY family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	474,717	0	C	T	99.1%	1199.4 / NA	333	P97S (CCA→TCA)	ybaY	YbaY family lipoprotein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (188/142); minor base G (3/0); total (191/142)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

GGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAG  >  NZ_CP025268/474582‑474851

 gTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGtcacca                                                                                                                                   >  1:138399/1‑140 (MQ=255)

   aCCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAGAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCAtt                                                                                                                                 <  2:5228863/140‑1 (MQ=255)

   aCCGTCTGGAGCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCAtt                                                                                                                                 <  2:3077545/140‑1 (MQ=255)

     cGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATGCa                                                                                                                               >  1:125523/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  2:5559909/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  2:133057/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  2:1780379/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  1:3574752/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  2:274906/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  2:5152027/1‑140 (MQ=255)

       tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGc                                                                                                                             >  2:297755/1‑140 (MQ=255)

         tGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGGGACACTTTCTGACGCGTCGTTAGCCGATGCGCCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCtt                                                                                                                           >  2:4928734/1‑140 (MQ=255)

            aTCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGt                                                                                                                        >  1:3831335/1‑140 (MQ=255)

              ccGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAACCAGTCACCATTCAGCTTTGTTc                                                                                                                      <  1:3618108/140‑1 (MQ=255)

               cGTCAGAAAGTCGCACTGTCGCCTGATGCTGTGCTGGCCGTGACACTTTCTGCCGCGTCGTTAGCCGATGCACCGTAAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCt                                                                                                                     <  1:2148551/140‑1 (MQ=255)

                gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTg                                                                                                                    <  1:2360692/140‑1 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGGTAAACAGTCACCATTCAGCTTTGTTCTg                                                                                                                    >  2:4680492/1‑140 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTGAGCTTTGTTCTGt                                                                                                                   >  2:3580173/1‑140 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                   >  1:1796907/1‑140 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                   >  2:3464053/1‑140 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                   >  2:5383376/1‑140 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                   >  1:191694/1‑140 (MQ=255)

                 tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTACTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                   >  1:4668719/1‑140 (MQ=255)

                 tCAGAAAGACGCACTGCCGCCTGATGCTGTGCTGACCTTGACACTTTCTGACGCGGCGTTAGCCGATGCACAGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGGTAAACAGTCACCATTCAGCTTTGTTCTg                                                                                                                    >  2:664757/1‑140 (MQ=255)

                   aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCa                                                                                                                 <  2:5046558/140‑1 (MQ=255)

                     aaaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCAtt                                                                                                               >  1:3028896/1‑140 (MQ=255)

                     aaaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCAtt                                                                                                               <  2:3909749/140‑1 (MQ=255)

                      aaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCAttt                                                                                                              <  1:4351574/140‑1 (MQ=255)

                           gCACTGCCGCATGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTCGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAccc                                                                                                         <  2:110556/140‑1 (MQ=255)

                              cTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                                      <  1:5224447/140‑1 (MQ=255)

                                           ctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCg                                                                                         >  2:3206139/1‑140 (MQ=255)

                                           ctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCg                                                                                         >  1:2136576/1‑140 (MQ=255)

                                              tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                      >  1:4471651/1‑140 (MQ=255)

                                              tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                      >  2:4181506/1‑140 (MQ=255)

                                              tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCAACGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                      >  1:3103973/1‑140 (MQ=255)

                                               gctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑CGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcg                                                                                     <  1:2291409/140‑1 (MQ=255)

                                                ctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                    >  1:4072449/1‑140 (MQ=255)

                                                  gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                  >  2:1460810/1‑140 (MQ=255)

                                                  gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                  <  1:3259845/140‑1 (MQ=255)

                                                  gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                  <  1:989641/140‑1 (MQ=255)

                                                   aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgcg                                                                                 >  2:1856100/1‑140 (MQ=255)

                                                   aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGACAGATGTTCAGCCGAAcgcgc                                                                                  >  2:750839/1‑139 (MQ=255)

                                                   aCCGTGACACTTTCTGACGCGGCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGGGCGGACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgcg                                                                                 >  2:4966014/1‑140 (MQ=255)

                                                     cGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTa                                                                               >  2:4208561/1‑140 (MQ=255)

                                                     cGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTa                                                                               >  1:5121689/1‑140 (MQ=255)

                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAGGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCGGGCAGATGTTCAGCCGAACGCGCGTAGTc                                                                            >  2:2681873/1‑140 (MQ=255)

                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                            >  1:2926793/1‑140 (MQ=255)

                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                            >  1:4924222/1‑140 (MQ=255)

                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                            >  2:1239781/1‑140 (MQ=255)

                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                            >  1:1137674/1‑140 (MQ=255)

                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCGGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATCCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                            >  2:1974582/1‑140 (MQ=255)

                                                            cTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGtt                                                                        <  1:76830/140‑1 (MQ=255)

                                                             tttCTGACGCGTCGTTAGCCTATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGGA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTTTTCTGTTg                                                                       >  2:2457959/1‑140 (MQ=255)

                                                               tCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAg                                                                     >  1:600421/1‑140 (MQ=255)

                                                                cTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGt                                                                    <  1:5042117/140‑1 (MQ=255)

                                                                  gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                                  <  1:1000697/140‑1 (MQ=255)

                                                                    cgcgTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgcgg                                                                >  1:520044/1‑140 (MQ=255)

                                                                    cgcgTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgcgg                                                                <  1:1603513/140‑1 (MQ=255)

                                                                       gtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                                  >  2:3407205/1‑135 (MQ=255)

                                                                        tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGAt                                                            >  1:1896569/1‑140 (MQ=255)

                                                                         cgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGAtt                                                           >  2:1713601/1‑140 (MQ=255)

                                                                                  aTGCACCGTCAAAAGTGCTGGCGGAGCAAGCGGTGCGTACTGAA‑GGTAAACGGTCACCATTCAGCTTTGTTCGGTCATTTAACCCGGCAGCTGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAt                                                  >  1:3349691/1‑140 (MQ=255)

                                                                                  aTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTAtt                                                                             <  1:4270270/113‑1 (MQ=255)

                                                                                  aTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAt                                                  >  2:458715/1‑140 (MQ=255)

                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACGATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATg                                                 >  2:3881019/1‑140 (MQ=255)

                                                                                    gCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGa                                                <  2:4291122/140‑1 (MQ=255)

                                                                                      aCCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTACCCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACa                                              <  2:4721674/140‑1 (MQ=255)

                                                                                      aCCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACa                                              <  2:2992564/140‑1 (MQ=255)

                                                                                        cGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAGGACaaa                                            >  1:1676611/1‑140 (MQ=255)

                                                                                         gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTGCCGTGAATGACAAAc                                           >  2:4461608/1‑140 (MQ=255)

                                                                                         gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAAc                                           <  1:39087/140‑1 (MQ=255)

                                                                                         gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAAc                                           >  1:4947861/1‑140 (MQ=255)

                                                                                                 tGCTGGCGCAGACAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt                                   <  1:111993/140‑1 (MQ=255)

                                                                                                 tGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt                                   <  1:5440305/140‑1 (MQ=255)

                                                                                                 tGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt                                   <  1:4191789/140‑1 (MQ=255)

                                                                                                   cTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTAt                                 >  1:4002537/1‑140 (MQ=255)

                                                                                                    tGGCGCATAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTAtt                                >  2:2186718/1‑139 (MQ=255)

                                                                                                    tGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATc                                >  1:1977354/1‑140 (MQ=255)

                                                                                                    tGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATc                                >  1:2651025/1‑140 (MQ=255)

                                                                                                    tGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATc                                >  1:3761465/1‑140 (MQ=255)

                                                                                                    tGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTGATc                                >  1:4529204/1‑140 (MQ=255)

                                                                                                        gcAGAAAGCCGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTACCCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCCCCg                            <  2:2266344/140‑1 (MQ=255)

                                                                                                         cAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGa                           <  1:5526331/140‑1 (MQ=255)

                                                                                                         cAGAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGa                           <  1:5348407/140‑1 (MQ=255)

                                                                                                           gAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATa                         >  2:2820651/1‑140 (MQ=255)

                                                                                                           gAAAGCGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATa                         >  2:3800265/1‑140 (MQ=255)

                                                                                                                cGGTGCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGtt                    <  1:2538856/140‑1 (MQ=255)

                                                                                                                   tGCGTACTGCA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTCACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAg                 >  2:5269196/1‑140 (MQ=255)

                                                                                                                    gCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGc                <  1:2043928/140‑1 (MQ=255)

                                                                                                                    gCGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGc                <  1:24326/140‑1 (MQ=255)

                                                                                                                     cGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTACCCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGcc               <  2:2766667/140‑1 (MQ=255)

                                                                                                                     cGTACTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGcc               <  1:2751036/140‑1 (MQ=255)

                                                                                                                         cTGAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTg           <  1:3479454/140‑1 (MQ=255)

                                                                                                                           gAA‑GGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGAt         <  2:818937/140‑1 (MQ=255)

                                                                                                                                gTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAc     >  1:2842072/1‑140 (MQ=255)

                                                                                                                                gTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAc     <  2:4471651/140‑1 (MQ=255)

                                                                                                                                  aaaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCa   <  1:2831726/140‑1 (MQ=255)

                                                                                                                                  aaaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCa   >  1:4752812/1‑140 (MQ=255)

                                                                                                                                  aaaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTACAGCCGGTGATCAACCa   >  2:1594042/1‑140 (MQ=255)

                                                                                                                                   aaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAg  >  1:681067/1‑140 (MQ=255)

GGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAA‑GGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAG  >  NZ_CP025268/474582‑474851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`