BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	485,691	C→T	P36S (CCA→TCA)	mscK →	mechanosensitive channel MscK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	485,691	0	C	T	100.0%	834.3 / NA	229	P36S (CCA→TCA)	mscK	mechanosensitive channel MscK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (114/115); total (114/115)

Only 100 of 229 total aligned reads displayed.

CGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAA  >  NZ_CP025268/485555‑485826

  ttcttcAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCg                                                                                                                                    >  1:4874758/1‑140 (MQ=255)

    cttaAGGTTCAGACACCTTCATTCATCATGTCTATGTTCCAGTATTGCAAACGTTCGCGGCATTTGGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGaa                                                                                                                                  <  2:4206405/140‑1 (MQ=255)

          gTTCAGAATCCTTCATTGATCATGACTATGTTCCAGTATTACAAACGATCACGGTATGTGTGGGGTTGAGCTTTCGTTGCTTTTGTTTGTGTCTGGTTAGGCCAGAACGCGGCGTTTGCGGGTGAGTCATCGAATGGTGa                                                                                                                            >  2:35285/1‑140 (MQ=255)

           ttCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGAt                                                                                                                           <  1:1709109/140‑1 (MQ=255)

                   ccTTCATTCATCATGTCTATTTTCCAGTATTACAAACGCTCACGGCTTTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTCTGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGAc                                                                                                                   <  2:1746354/140‑1 (MQ=255)

                   ccTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGAc                                                                                                                   <  1:5495314/140‑1 (MQ=255)

                    cTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTTTTGCTTTTGGCTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTGATCGAGCGGTGATCTGTCGACa                                                                                                                  <  2:3795266/140‑1 (MQ=255)

                          tcatcaTGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCg                                                                                                            >  1:1614460/1‑140 (MQ=255)

                            atcatGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGa                                                                                                          <  2:3874539/140‑1 (MQ=255)

                            atcatGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGa                                                                                                          <  2:4453599/140‑1 (MQ=255)

                              catGACTATGTTCCAGTATTACACACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTGTTTGTCGTGTTATGGCAGAACACGGCGTTTGCGCGGGCGTCTTCGAATGGTGATCTGCCGACAAAAGCGGAcc                                                                                                        >  2:123575/1‑140 (MQ=255)

                              catGACTATGTTCCAGTATTACAAACGATCACGGCCTTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGAcc                                                                                                        >  2:439039/1‑140 (MQ=255)

                              catGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGAcc                                                                                                        >  2:525219/1‑140 (MQ=255)

                              catGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGAcc                                                                                                        >  1:2665591/1‑140 (MQ=255)

                              catGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGAcc                                                                                                        >  1:1377743/1‑140 (MQ=255)

                              catGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGAcc                                                                                                        >  1:3038485/1‑140 (MQ=255)

                              catGACTATGTTCCAGGATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCCAATGGTGATCTGCCGACAAAAGCAGAcc                                                                                                        >  1:2674212/1‑140 (MQ=255)

                                   cTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAg                                                                                                   >  2:2830802/1‑140 (MQ=255)

                                      tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                                >  1:3617579/1‑140 (MQ=255)

                                      tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                                <  1:2178679/140‑1 (MQ=255)

                                      tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                                >  1:83957/1‑140 (MQ=255)

                                      tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                                <  2:923811/140‑1 (MQ=255)

                                        ttCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCa                                                                                              >  1:181126/1‑140 (MQ=255)

                                        ttCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCa                                                                                              >  2:2775182/1‑140 (MQ=255)

                                            aGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACtt                                                                                          >  1:3489408/1‑140 (MQ=255)

                                              tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGa                                                                                        >  1:1982831/1‑140 (MQ=255)

                                                ttACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACt                                                                                      >  1:2479065/1‑140 (MQ=255)

                                                ttACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACt                                                                                      >  1:291098/1‑140 (MQ=255)

                                                  aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCa                                                                                    >  1:1001662/1‑140 (MQ=255)

                                                        gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAgg                                                                                                  <  1:4489081/120‑1 (MQ=255)

                                                        gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAgg                                                                                                  >  2:4489081/1‑120 (MQ=255)

                                                        gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaat                                                                              >  2:4539634/1‑140 (MQ=255)

                                                        gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaat                                                                              >  2:613114/1‑140 (MQ=255)

                                                        gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaat                                                                              >  2:2853287/1‑140 (MQ=255)

                                                        gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTCTTGTTTTTGTCCTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCGGGCGCAACTTGACTCACtaaat                                                                              >  2:2542411/1‑140 (MQ=255)

                                                         aTCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                             <  1:2183850/140‑1 (MQ=255)

                                                          tCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaataa                                                                            >  2:3967106/1‑140 (MQ=255)

                                                          tCACGGCATTTGGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGCCGCAACTTGACTCACtaaataa                                                                            >  2:3985902/1‑140 (MQ=255)

                                                           cACGGCATTTTGTTTTTTCAGCTTTTATTGCTTTTGTTTTTGTCTTGTTATTCCAGAAGACGGCGTTTGCGCGGGCGTCATCGAATGGGGATCTGCCGACAAAATGGGACCTGCAGGCTCTACTTGACTCACtaaataaa                                                                           >  2:1926436/1‑140 (MQ=255)

                                                           cACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaataaa                                                                           >  1:1481209/1‑140 (MQ=255)

                                                              ggCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaa                                                                        >  1:5222272/1‑140 (MQ=255)

                                                                 aTTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaaaaa                                                                     <  1:1894587/140‑1 (MQ=255)

                                                                  ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                             <  2:5036890/131‑1 (MQ=255)

                                                                   tttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGa                                                                   >  1:2445479/1‑140 (MQ=255)

                                                                   tttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGa                                                                   <  2:666155/140‑1 (MQ=255)

                                                                        tttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                              <  2:2513441/140‑1 (MQ=255)

                                                                        tttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                              <  2:1867033/140‑1 (MQ=255)

                                                                        tttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                              <  1:4015780/140‑1 (MQ=255)

                                                                        tttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                              <  1:3050345/140‑1 (MQ=255)

                                                                         ttttCAGCATTTATTGCTTTTGATTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTc                                                             >  1:4259572/1‑140 (MQ=255)

                                                                            tCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGc                                                          <  1:5409335/140‑1 (MQ=255)

                                                                             catcATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATTAACAAAAAGATCTTTCTGCt                                                         <  2:714842/137‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  1:5043279/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  1:2419373/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  2:2225208/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  2:4924509/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  1:3791892/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  2:1389817/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  2:2907869/140‑1 (MQ=255)

                                                                             cAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                         <  2:4304465/140‑1 (MQ=255)

                                                                                 aTTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAAGAAACAAAAAGATCTTTCTGCTCAgg                                                     >  2:4790764/1‑140 (MQ=255)

                                                                                  tttATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGa                                                    <  2:3086901/140‑1 (MQ=255)

                                                                                       tGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAAc                                               <  2:821998/140‑1 (MQ=255)

                                                                                          ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTgg                                            >  1:1045099/1‑140 (MQ=255)

                                                                                          ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTgg                                            <  2:2340335/140‑1 (MQ=255)

                                                                                          ttttgtttgtgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTTAGGACAAACTgg                                            >  2:3951262/1‑140 (MQ=255)

                                                                                            ttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTg                                          >  1:5125061/1‑140 (MQ=255)

                                                                                            ttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTg                                          >  2:4121243/1‑140 (MQ=255)

                                                                                              gtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTACATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgca                                        >  2:3026557/1‑140 (MQ=255)

                                                                                               tttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                                       <  2:83957/140‑1 (MQ=255)

                                                                                               tttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                                       <  2:1062665/140‑1 (MQ=255)

                                                                                                ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcagc                                      <  1:3811722/140‑1 (MQ=255)

                                                                                                 tttgtCTTGTTATGCCAGAACACGGCGTGTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcagca                                     <  2:332610/140‑1 (MQ=255)

                                                                                                   tgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAgg                                   >  1:1314067/1‑140 (MQ=255)

                                                                                                     tCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGAt                                 <  2:1614460/140‑1 (MQ=255)

                                                                                                     tCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGAt                                 <  1:774733/140‑1 (MQ=255)

                                                                                                     tCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGAt                                 <  1:459827/140‑1 (MQ=255)

                                                                                                      cTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATc                                <  1:2628913/140‑1 (MQ=255)

                                                                                                       ttGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCt                               <  1:1051959/140‑1 (MQ=255)

                                                                                                         gTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGCTCTGa                             >  2:4901802/1‑140 (MQ=255)

                                                                                                         gTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGa                             >  2:5086507/1‑140 (MQ=255)

                                                                                                            aTGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGTTCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATGTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                          <  1:72152/140‑1 (MQ=255)

                                                                                                            aTGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                          <  1:525219/140‑1 (MQ=255)

                                                                                                            aTGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                          <  2:2401392/140‑1 (MQ=255)

                                                                                                             tGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGa                         <  1:1933380/140‑1 (MQ=255)

                                                                                                               ccAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATa                       >  1:1147633/1‑140 (MQ=255)

                                                                                                                 aGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGc                                                          >  1:3334705/1‑103 (MQ=255)

                                                                                                                    acacGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTa                  >  1:4342349/1‑140 (MQ=255)

                                                                                                                      acGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGc                <  2:3241531/140‑1 (MQ=255)

                                                                                                                       cGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGCCCAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGcc               >  1:4311680/1‑140 (MQ=255)

                                                                                                                          cGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc            <  1:2775182/140‑1 (MQ=255)

                                                                                                                          cGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc            <  1:3728028/140‑1 (MQ=255)

                                                                                                                          cGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc            <  2:2665591/140‑1 (MQ=255)

                                                                                                                          cGTTTGCGCGGGCGTCAGCGAATGGTGATCTGACGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACATACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc            <  1:2600896/140‑1 (MQ=255)

                                                                                                                          agTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc            <  1:4925994/139‑1 (MQ=255)

                                                                                                                             ttGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTc         >  1:2737488/1‑140 (MQ=255)

                                                                                                                             ttGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTc         >  1:631896/1‑140 (MQ=255)

                                                                                                                                    ggCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATaaaa  >  2:5318361/1‑140 (MQ=255)

                                                                                                                                    ggCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGAGaaaa  >  2:2400449/1‑140 (MQ=255)

                                                                                                                                    ggCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTGGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATaaaa  >  1:1441368/1‑140 (MQ=255)

CGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAA  >  NZ_CP025268/485555‑485826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`