BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	93.9%	413.6 / 15.7	98	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (4/2); new base . (57/35); total (61/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	95.6%	370.2 / 22.3	98	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (4/0); major base . (52/35); minor base T (2/2); total (61/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728663‑3728932

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cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTATCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    <  2:4418918/140‑1 (MQ=255)

 tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccc                                                                                                                                   <  1:933454/140‑1 (MQ=255)

   aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGcccccc                                                                                                                                 >  2:3615448/1‑140 (MQ=255)

   aTAAGCGACATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                                                                 >  2:3253609/1‑140 (MQ=255)

        cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                            <  1:3778923/140‑4 (MQ=255)

          gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacg                                                                                                                          >  1:4398133/1‑135 (MQ=255)

             tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTa                                                                                                                     >  1:4080143/1‑140 (MQ=255)

                  cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCC‑‑TACGGTAATCgg                                                                                                                >  2:1645100/1‑139 (MQ=255)

                  cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGa                                                                                                                >  2:3395194/1‑140 (MQ=255)

                   tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATTTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               >  2:2322178/1‑140 (MQ=255)

                   tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               <  1:147343/140‑1 (MQ=255)

                   tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               >  2:2321369/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCTGTGCTGGCCCCACAT‑‑CCCCCCCCGTACTGTAATCGGgct                                                                                                             >  2:412438/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCTGTACCGTAATCGAgct                                                                                                             >  2:4244751/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                             >  1:3009050/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑CCCGCCCCCGTACCGCAATCggcgt                                                                                                             >  2:3287592/1‑136 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTCGCTGCGCCCAGTCCTGGCCCAAC‑‑CTCCCCCCCCGTACGGTAATCGAgct                                                                                                             >  1:2640544/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGGATTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAACCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCAGTACGGTAATTGAgct                                                                                                             >  1:162267/1‑140 (MQ=255)

                       cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCTAGCCTTGCTGCGCCCAGTGCTGGCGCCACAT‑‑CCCCCTCCGTACGGTACTTGAgcgtt                                                                                                           >  2:179141/1‑137 (MQ=255)

                        aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                          <  1:2452232/140‑1 (MQ=255)

                         tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGctgctg                                                                                                         >  2:1618667/1‑140 (MQ=255)

                          cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGTTAATCGAGCTGCTGa                                                                                                        >  2:1431551/1‑140 (MQ=255)

                          cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                        >  2:4134963/1‑140 (MQ=255)

                          cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                        <  2:4080143/140‑1 (MQ=255)

                             gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                     <  1:4134963/140‑1 (MQ=255)

                             gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGCAATCTCGCTGCTGACCg                                                                                                     >  2:4063480/1‑140 (MQ=255)

                               aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                   >  1:4555377/1‑140 (MQ=255)

                               aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCCGCCGGCCGcc                                                                                                   >  2:4533147/1‑139 (MQ=255)

                                  tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGat                                                                                                >  2:5097113/1‑140 (MQ=255)

                                  tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑ACCCCCCCCGTACAGTAATCGGCCTGCTCCCCGATGag                                                                                                >  1:4117408/1‑139 (MQ=255)

                                      aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑TCTCCCCCGGACGGTAATCAAACTGCTGACCGCTGATATcc                                                                                            >  1:2866217/1‑140 (MQ=255)

                                        tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGc                                                                                          >  1:4535310/1‑140 (MQ=255)

                                        tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTGCTCTGGGTTCGCCGCGAGCTGCGCCAGCCTGGCTGCGCCCAGTGCGGGCCCCACAT‑‑TCCGCCCCGTACGGTGATCTTTCTGCGCACCGCCGatagcggc                                                                                          >  1:1095832/1‑135 (MQ=255)

                                          ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGAGATCCGCCa                                                                                        >  2:349870/1‑140 (MQ=255)

                                          ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACCGCAATGGCGCTGCTGCCCGCCGGTGTCCGCCa                                                                                        >  1:4302678/1‑140 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:2982807/140‑1 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATGCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:4731324/140‑1 (MQ=255)

                                                cGGTAGTCGCCGCAACAATCCAATGAGCGACTTCTCCGGATTCGCCGCGATCTGCGCCAGCCTTGCCGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACCGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:1097564/140‑1 (MQ=255)

                                                  gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:3201245/140‑1 (MQ=255)

                                                  gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:3085379/140‑1 (MQ=255)

                                                  gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCCCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  2:629111/140‑1 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  1:5059678/140‑1 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              >  1:4839165/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  1:1491766/140‑1 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              >  1:4096347/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATACGCCAGCATCTGACg                                                                              >  2:3627705/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTACTGACCGCTGAAATCCGCCAGCATCTGACg                                                                              >  1:3149180/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCTGATATCCGCCGGCATCTGACg                                                                              >  1:4839422/1‑140 (MQ=255)

                                                     gTTTCGGCAACAATTCAATGAGCGATTTCTCTGGATCCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACTGTAATCGAGCTGCTGACCGCTGATCTCCGCCAGCATCTGACGc                                                                             <  2:2370249/140‑1 (MQ=255)

                                                     gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  2:579668/140‑1 (MQ=255)

                                                      ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGGTGATCTCCGCCCGCATCTGACGcc                                                                            >  1:677086/1‑140 (MQ=255)

                                                        gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                          <  1:4474825/140‑1 (MQ=255)

                                                        gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                          <  1:4474854/140‑1 (MQ=255)

                                                         cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                         <  2:2128097/140‑1 (MQ=255)

                                                          ggCAACAATTCAATGAGCGATTTCTCTGGTTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTCGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        <  2:3564214/140‑1 (MQ=255)

                                                          ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        <  2:2711661/140‑1 (MQ=255)

                                                           gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTACGGTAGTCGGGCTGCTGACCGCTGATATCCGCAAGCATCTGACGCCAGTAc                                                                       >  1:860081/1‑140 (MQ=255)

                                                           gCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCGACA‑‑GACCCCCCCGTACGGTAATCGAGCTGCTGACCGCTTGTAGCCGCCAGCGTAGGACGCCAGtgc                                                                       >  1:3029602/1‑138 (MQ=255)

                                                            aaaccaTTCAAGGAGCGATTGCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCAAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                      <  2:2050777/139‑1 (MQ=255)

                                                             aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGTCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                     <  2:3840138/140‑1 (MQ=255)

                                                                 aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                 <  1:1589129/140‑1 (MQ=255)

                                                                 aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCAGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                 <  2:230099/140‑1 (MQ=255)

                                                                        gAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCGTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACCATAATCGAGCTGCTGACCGCTGATATCCGCCTGCATCTGTCGCCAGTACTCAATACGcgccc                                                          <  2:1284294/140‑1 (MQ=255)

                                                                          gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                        <  2:5093795/140‑1 (MQ=255)

                                                                          gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCCGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                        >  1:5066197/1‑140 (MQ=255)

                                                                             aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                     <  1:274078/140‑1 (MQ=255)

                                                                                  tctGGAATCGCCGCGGTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGTCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  2:5021500/140‑1 (MQ=255)

                                                                                  cctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCATTACTCACTACGCGCCCCGCCCCCAAt                                                <  2:318017/139‑1 (MQ=255)

                                                                                    tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGGCGGTAATCGAGCCGCTGACCCCTGATATCCGCCAGCATCTGACGCCAGTACTCACTATGCGCCCCGCCCCCAATCa                                              >  1:1066526/1‑140 (MQ=255)

                                                                                        ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCGCAT‑‑CTCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                          >  1:4360202/1‑140 (MQ=255)

                                                                                         tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                         >  1:1512221/1‑140 (MQ=255)

                                                                                         tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                         >  1:1511680/1‑140 (MQ=255)

                                                                                           gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCCGACCGCTGATATCCGCCAGCATCTGCCGCCAGTACTCACTACCCGCCCCGCCCCCACCCAACCTAac                                       >  1:1076761/1‑140 (MQ=255)

                                                                                           gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTAGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCCGATACCCGCCAGCAGCTGACGCCAGTACTCACCACGCACCCCGCTCCCAATCACTGTAac                                       >  2:1641067/1‑140 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACTCAGCGCCCCGCCCCCCATCAACGTCACACTTTg                                 >  2:4218695/1‑140 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  1:179281/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  1:777698/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCAGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 >  2:4218706/1‑140 (MQ=255)

                                                                                                      cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        >  1:4152452/1‑96 (MQ=255)

                                                                                                      cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        >  1:4152631/1‑96 (MQ=255)

                                                                                                      cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        <  2:4152631/96‑1 (MQ=255)

                                                                                                      cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        <  2:4152452/96‑1 (MQ=255)

                                                                                                           gCCTTGCTGCGCCCCGTGCTGGCCCCACAT‑‑CCACCCCCGTAAGGTGAGGGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCACCCCCCATCACCGTAACACTTTGCGGTTTGATa                       >  1:2655175/1‑140 (MQ=255)

                                                                                                           gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                       >  1:2655242/1‑140 (MQ=255)

                                                                                                            ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                      <  2:860839/140‑1 (MQ=255)

                                                                                                            ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                      >  2:1320337/1‑140 (MQ=255)

                                                                                                            ccTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCCC‑TACGGTAAACGAGGTGCTGACCGCTGGTATCCGCCCGCATCTGACGCCAATACTCACTACGCGCCCCACCCCCAATCAACGGAACACTTTGCGGCTTAATAc                      >  1:3083884/1‑140 (MQ=255)

                                                                                                            ccTTGCTGCGCCCAGTGCTGGCCCCACA‑‑ACCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCCCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAACCAACGTAACACTTTGCGGTTTGATAc                      >  1:2552224/1‑140 (MQ=255)

                                                                                                              ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                    <  2:411661/140‑1 (MQ=255)

                                                                                                                gctgcGCCCAGTGCTGGCCCCACA‑‑CCCCCCCCCGTACGGTGATCGAGGTGCTTACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTGACTACGCGCCCCGCCCCCCACCCACACAACACTTTGCAGGTTAATACCGcc                  >  2:2808251/1‑139 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAACCGAGGTGCCGACCGCTCATACCCCCCACCAGCCGACGCCAGCACTCACCACGCCCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATCCCGCAGGGATg            >  2:4137224/1‑140 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCCCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg            >  2:5094310/1‑140 (MQ=255)

                                                                                                                         aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGTGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGcgc         >  1:4350665/1‑138 (MQ=255)

                                                                                                                          gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCAGACCGCCGATATACGCCCGCCTCCGCCGCCAGTCCTCCCTACGCGCCCCGCCCCCCATCAACCTAACACTTTCCGACTTAATACCGCAGCCATGCAcc        >  2:2832481/1‑139 (MQ=255)

                                                                                                                           tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCTGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa       >  2:4705010/1‑140 (MQ=255)

                                                                                                                           tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa       >  1:8801/1‑140 (MQ=255)

                                                                                                                           tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa       >  2:1374217/1‑140 (MQ=255)

                                                                                                                           tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGCGCCGCCAGCATCTGACGCCAGGACACACCACGCGCCCCCCCCCCAATCCACGTAACACATTGCGGTTTAAGACGGCAGGCAGGCACGa       >  1:5085342/1‑140 (MQ=255)

                                                                                                                             cTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa     <  1:3395194/140‑1 (MQ=255)

                                                                                                                                gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAACAACGTAACACTTTTCGGTTTAATACCGCAGGCATGCACGACAtac  >  2:2098405/1‑138 (MQ=255)

||

CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728663‑3728932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`