BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	674,649	+T	pseudogene (947/977 nt)	ybeQ ←	SEL1‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	674,649	1	.	T	98.7%	1180.3 / 3.8	318	pseudogene (947/977 nt)	ybeQ	SEL1‑like repeat protein
Reads supporting (aligned to +/- strand): ref base . (3/1); new base T (136/178); total (139/179)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

GGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTT‑C‑C‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGCA  >  NZ_CP025268/674511‑674779

 gTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑c‑ctg                                                                                                                                   >  1:2682451/1‑138 (MQ=255)

      gACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑ctgactga                                                                                                                              >  2:767588/1‑140 (MQ=255)

       aCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTgactgac                                                                                                                             <  1:3089981/140‑1 (MQ=255)

         cAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGACTTT‑C‑CTGCCTGACAt                                                                                                                           >  2:3503845/1‑140 (MQ=255)

            ttCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACAtctc                                                                                                                        >  2:2362521/1‑140 (MQ=255)

            ttCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACAtctc                                                                                                                        <  2:2431497/140‑1 (MQ=255)

            ttCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACAtctc                                                                                                                        >  1:741018/1‑140 (MQ=255)

            ttCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACAtctc                                                                                                                        >  1:1697087/1‑140 (MQ=255)

            ttCCAGAGACGACCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCGCACGAATTT‑C‑CTGACTGACAtctc                                                                                                                        <  2:4716202/140‑1 (MQ=255)

                 gagaCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAAAAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGtt                                                                                                                   >  2:4672212/1‑140 (MQ=255)

                    aCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCg                                                                                                                <  2:607822/140‑1 (MQ=255)

                    aCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCg                                                                                                                <  1:1985583/140‑1 (MQ=255)

                      gCCCGTTTGAGCCGTAATCGTCTGATCTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAACTT‑C‑CTGACTGACATCTCTGGTTCCGGa                                                                                                              >  2:1824440/1‑140 (MQ=255)

                      gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTTGTTCCGGa                                                                                                              >  2:3457098/1‑140 (MQ=255)

                      gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGa                                                                                                              >  2:2530140/1‑140 (MQ=255)

                      gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGa                                                                                                              <  2:4614297/140‑1 (MQ=255)

                      gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGa                                                                                                              >  1:4524291/1‑140 (MQ=255)

                      gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGa                                                                                                              >  2:4865775/1‑140 (MQ=255)

                      gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGa                                                                                                              >  1:3395326/1‑140 (MQ=255)

                      gCCCGTTGGAACCGTAATAGTCTGATTTCGCACAGCAATAAAGCGAATATGGGCCGAATGAAATTCACCAGCCCTTGATTTTATCTCTACAGTAAATCGTCAGTCTCAAGAATTT‑CAT‑GACTAACGTCTCTgggtaagaa                                                                                                              >  2:1135775/1‑133 (MQ=255)

                       cccGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGAt                                                                                                             >  1:2271430/1‑140 (MQ=255)

                         cGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGa                                                                                                           <  2:2720381/140‑1 (MQ=255)

                            ttGAGCCGTAATAGTCTGATTTCGCAAAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTg                                                                                                        <  1:3606378/140‑1 (MQ=255)

                              gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                      <  1:212091/140‑1 (MQ=255)

                              gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                      <  1:1385629/140‑1 (MQ=255)

                              gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                      <  2:738626/140‑1 (MQ=255)

                              gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                      <  1:2894698/140‑1 (MQ=255)

                              gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTACGGATGACTGaa                                                                                                      <  2:5048227/140‑1 (MQ=255)

                                   gTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTa                                                                                                 <  1:3475198/140‑1 (MQ=255)

                                   gTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTa                                                                                                 <  2:1442887/140‑1 (MQ=255)

                                   gTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTa                                                                                                 <  2:3081490/140‑1 (MQ=255)

                                    tAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTAc                                                                                                <  1:3318500/140‑1 (MQ=255)

                                        aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                                            <  2:3947892/140‑1 (MQ=255)

                                        aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                                            <  1:4186832/140‑1 (MQ=255)

                                        aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                                            <  1:162245/140‑1 (MQ=255)

                                          tCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAGATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTGAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGAATGAACTTTACGATACa                                                                                          >  1:2829579/1‑140 (MQ=255)

                                          tCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACa                                                                                          >  2:3674129/1‑140 (MQ=255)

                                              aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATTTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                      >  2:2572938/1‑140 (MQ=255)

                                              aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                      >  2:3833741/1‑140 (MQ=255)

                                              aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                      >  2:693950/1‑140 (MQ=255)

                                                  cGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCgaga                                                                                  <  1:2773459/140‑1 (MQ=255)

                                                    cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                                >  1:3559327/1‑140 (MQ=255)

                                                    cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                                <  2:1194848/140‑1 (MQ=255)

                                                    cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                                >  1:846626/1‑140 (MQ=255)

                                                                   aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTTAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                 >  2:1696268/1‑140 (MQ=255)

                                                                   aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCCCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                 >  1:2921142/1‑140 (MQ=255)

                                                                   aaTATGGGCCGAATGAAATTCCCCGACCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                 >  2:4562155/1‑140 (MQ=255)

                                                                        gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                                            >  1:80486/1‑140 (MQ=255)

                                                                          gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTctt                                                          >  1:1876581/1‑140 (MQ=255)

                                                                          gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTA‑C‑CTGACTGACATCTCTGGTTGCGGATGACTGAAATTTACGATACAGGTGGAGAGCCTGTCGGAGATCGACAGGTctt                                                          >  2:3267476/1‑140 (MQ=255)

                                                                           ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                         >  1:4301580/1‑140 (MQ=255)

                                                                           ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTGCAGTCTCACGAATTT‑C‑CTGACAGACATCTCGGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCGGTCGGAGATCGACAGGTcttc                                                         >  2:4494035/1‑140 (MQ=255)

                                                                             gAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcct                                                       <  1:1026770/140‑1 (MQ=255)

                                                                              aaTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                                                      <  2:3985357/140‑1 (MQ=255)

                                                                                   aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                 <  1:523931/140‑1 (MQ=255)

                                                                                   aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                 <  1:1533906/140‑1 (MQ=255)

                                                                                   aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                 <  1:2472266/140‑1 (MQ=255)

                                                                                   aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                 <  1:3992950/140‑1 (MQ=255)

                                                                                   aaTTCCCCGGCCCTTGTTATTACCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGCCTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                 <  2:3630893/140‑1 (MQ=255)

                                                                                    aTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGt                                                >  2:4342525/1‑140 (MQ=255)

                                                                                     ttCCCCGGCCCTTGTTTTTATTTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTa                                               <  2:2050569/140‑1 (MQ=255)

                                                                                     ttCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATCCAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTa                                               <  1:3683000/140‑1 (MQ=255)

                                                                                      tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGACTTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                              <  1:99353/140‑1 (MQ=255)

                                                                                      tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGCTGACTGAACTTTACGATACAGGTCGAGAGCCTCTCGGAGATCGACAGGTCTCCCTTCTCCGTAg                                              <  2:1746853/140‑1 (MQ=255)

                                                                                      tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACCGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                              <  1:694102/140‑1 (MQ=255)

                                                                                      tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                              <  2:2885548/140‑1 (MQ=255)

                                                                                        cccGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATAc                                                                                           >  2:4083431/1‑93 (MQ=255)

                                                                                        cccGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATAc                                                                                           <  1:4083431/93‑1 (MQ=255)

                                                                                         ccGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTgc                                           <  1:483772/140‑2 (MQ=255)

                                                                                         ccGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGa                                           <  2:2127797/140‑1 (MQ=255)

                                                                                          cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGACCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                          <  1:4392066/140‑1 (MQ=255)

                                                                                          cGGCCCTTGTTTTTATCTATACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACGGTCATCTCTGGTTCCGGATGACTGAACTTTACGATACTGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTACGTATCCGTAGTGAt                                          <  1:1463098/140‑1 (MQ=255)

                                                                                              ccTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACAt                                      <  2:1893976/140‑1 (MQ=255)

                                                                                               cTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATa                                     <  1:4679616/140‑1 (MQ=255)

                                                                                                   tttttATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATgg                                 <  2:1921837/140‑1 (MQ=255)

                                                                                                   tttttATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATgg                                 <  1:1220253/140‑1 (MQ=255)

                                                                                                    ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATTCAGGGCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                                <  2:925257/140‑1 (MQ=255)

                                                                                                    ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                                <  2:3303373/140‑1 (MQ=255)

                                                                                                    ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                                <  1:3662411/140‑1 (MQ=255)

                                                                                                    ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                                <  1:182978/140‑1 (MQ=255)

                                                                                                        acctctACTGTCAATCTTCATTCACATGGTTTTGC‑T‑GACTGACATTTCTGGTTATGGCTGACTGAACTTTACGATACAGGTCGAGAGCCTGGCGGCGATCGACAGGTTTTCCTTCTCCGTAGTGATACATCCTGGCCAgg                            <  2:4249582/135‑1 (MQ=255)

                                                                                                        aTCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                            <  2:2178906/140‑1 (MQ=255)

                                                                                                        aTCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                            <  2:1235317/140‑1 (MQ=255)

                                                                                                        aTCTCTACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                            <  2:556526/140‑1 (MQ=255)

                                                                                                          ctctACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGtt                          <  1:906528/137‑1 (MQ=255)

                                                                                                           tctACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTta                         <  1:3872939/138‑1 (MQ=255)

                                                                                                             tACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtata                       >  2:4582648/1‑140 (MQ=255)

                                                                                                             tACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtata                       <  1:3187984/140‑1 (MQ=255)

                                                                                                             tACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtata                       >  1:2591232/1‑140 (MQ=255)

                                                                                                             tACAGTAAATCTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtata                       >  1:1562178/1‑140 (MQ=255)

                                                                                                                  tAAATCTTCAGTCTCACGAATTT‑C‑CTGCCTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAgg                  <  2:1637120/140‑1 (MQ=255)

                                                                                                                       cTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTg             >  2:3145380/1‑140 (MQ=255)

                                                                                                                       cTTCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTg             >  1:1472682/1‑140 (MQ=255)

                                                                                                                        ttCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTgg            <  1:3248635/140‑1 (MQ=255)

                                                                                                                         tCAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGc           >  1:554717/1‑140 (MQ=255)

                                                                                                                          cAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCg          >  1:4893778/1‑140 (MQ=255)

                                                                                                                          cAGTCTCACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCg          >  1:1967778/1‑140 (MQ=255)

                                                                                                                               tcACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACGGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCAtt     <  1:1107586/140‑1 (MQ=255)

                                                                                                                                cACGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGAGGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAGACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTg    >  2:1570684/1‑140 (MQ=255)

                                                                                                                                  cGAATTT‑C‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGCa  >  1:4740995/1‑140 (MQ=255)

GGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTT‑C‑C‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGCA  >  NZ_CP025268/674511‑674779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`