BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	97.3%	837.8 / 11.4	224	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (5/1); new base A (117/101); total (122/102)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	1,224,094	2	.	G	97.3%	735.1 / 11.5	224	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (5/1); new base G (117/101); total (122/102)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NZ_CP025268/1223956‑1224223

||

cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGACTCCGTGCTCTCT‑‑T‑‑a                                                                                                                                  >  2:2373125/1‑140 (MQ=255)

 aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑ag                                                                                                                                 >  2:3324614/1‑139 (MQ=255)

   gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                               >  1:2663229/1‑137 (MQ=255)

   gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                               >  1:3227543/1‑137 (MQ=255)

   gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                               >  1:3367216/1‑137 (MQ=255)

    cTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaac                                                                                                                              <  1:2755690/140‑5 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTa                                                                                                                         <  2:1996013/140‑1 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTa                                                                                                                         <  1:3768084/140‑1 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTa                                                                                                                         <  1:1787175/140‑1 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTa                                                                                                                         <  2:3051048/140‑1 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAg                                                                                                                       >  2:499067/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAg                                                                                                                       >  2:3257407/1‑140 (MQ=255)

               cGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGt                                                                                                                     >  2:59268/1‑140 (MQ=255)

                 tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGttt                                                                                                                   <  2:4614805/140‑1 (MQ=255)

                 tttAAAAGATGAAACCAGTGTGATTCTTTATGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGttt                                                                                                                   <  2:627433/140‑1 (MQ=255)

                        gATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATa                                                                                                            <  2:398784/140‑1 (MQ=255)

                           gAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAAc                                                                                                         >  2:1030728/1‑140 (MQ=255)

                            aaaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGGTTTAGAATAAACa                                                                                                        <  1:445751/140‑1 (MQ=255)

                              acaaGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:577282/137‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      >  1:1758062/1‑140 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:2851452/140‑1 (MQ=255)

                                cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  2:1092715/140‑1 (MQ=255)

                                cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:1082052/140‑1 (MQ=255)

                                  gtgtGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCt                                                                                                  >  2:3978350/1‑140 (MQ=255)

                                        ttctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCa                                                                                            >  1:499413/1‑140 (MQ=255)

                                        ttctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCa                                                                                            <  2:3197880/140‑1 (MQ=255)

                                           tttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCa                                                                                         <  2:3274201/140‑1 (MQ=255)

                                             tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                       <  1:1330271/140‑1 (MQ=255)

                                                   ctctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATg                                                                                 >  1:4241329/1‑140 (MQ=255)

                                                    tctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGc                                                                                >  1:3960898/1‑140 (MQ=255)

                                                     ctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCa                                                                               <  1:4978592/140‑1 (MQ=255)

                                                      tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                              >  1:3315831/1‑140 (MQ=255)

                                                       aTCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             <  2:2227027/140‑1 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  2:1154838/140‑1 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:2503663/140‑1 (MQ=255)

                                                                tatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCAtt                                                                    >  1:899623/1‑140 (MQ=255)

                                                                  ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  2:2011611/140‑1 (MQ=255)

                                                                  ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  1:3994110/140‑1 (MQ=255)

                                                                  ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  1:3103043/140‑1 (MQ=255)

                                                                  ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  2:4502008/140‑1 (MQ=255)

                                                                    gCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAg                                                                >  2:71511/1‑140 (MQ=255)

                                                                          cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                          >  1:815964/1‑140 (MQ=255)

                                                                             tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTAttc                                                       >  2:2782632/1‑138 (MQ=255)

                                                                             tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                       >  1:1656974/1‑140 (MQ=255)

                                                                             tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                       >  1:3005411/1‑140 (MQ=255)

                                                                             tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCG‑‑TAGAACTTGAGTAAGGTTGTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                       >  1:2232716/1‑140 (MQ=255)

                                                                              gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:1238984/140‑1 (MQ=255)

                                                                              gAAATGCAGAAGACTACACTAACGGATATCATTATTAAGGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTAAGCATTTAAGGCGTTATGCt                                                      <  2:5051640/140‑1 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                  >  2:355252/1‑140 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                >  2:1870390/1‑140 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                >  2:5097166/1‑140 (MQ=255)

                                                                                      tAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                              >  1:3432420/1‑140 (MQ=255)

                                                                                            tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  1:3696794/140‑1 (MQ=255)

                                                                                            tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  2:1414563/140‑1 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  2:2904957/1‑140 (MQ=255)

                                                                                              cacTAACGGATATAATTATTAATGACAGGGAATCCGTGCTCACT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  2:978093/1‑140 (MQ=255)

                                                                                               acTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCa                                     >  2:3568936/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAGCATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGATTTCTTATTATGGCCGCAatat                                 >  2:90848/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  2:741162/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  1:1392430/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  1:607996/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  1:4188422/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  1:4331856/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  2:2872701/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTACGCAatat                                 >  1:2702293/1‑140 (MQ=255)

                                                                                                    cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                >  2:3617827/1‑140 (MQ=255)

                                                                                                    cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                >  1:1210804/1‑140 (MQ=255)

                                                                                                      gATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                              <  1:542571/140‑1 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            >  2:3823586/1‑140 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            >  2:3756628/1‑140 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            >  1:1505481/1‑140 (MQ=255)

                                                                                                         atCATTATTAATGACAGGGAATCCGTGCTCTCTGAG‑‑AACTTGAGTAAGGTTTTAGAATAAACATCGGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGATTTCTTATTATGTCTGCAATATCAtgtg                           >  2:4148658/1‑140 (MQ=255)

                                                                                                         atCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                           >  1:4564883/1‑140 (MQ=255)

                                                                                                          tCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCCTGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtgt                          <  2:482993/140‑1 (MQ=255)

                                                                                                          tCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtgt                          <  1:464363/140‑1 (MQ=255)

                                                                                                           cATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTc                         >  1:1098319/1‑140 (MQ=255)

                                                                                                           cATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTc                         <  1:59623/140‑1 (MQ=255)

                                                                                                             ttattaATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                       >  2:2523555/1‑140 (MQ=255)

                                                                                                             ttattaATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                       >  1:2013933/1‑140 (MQ=255)

                                                                                                                 taATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAAt                   >  1:614341/1‑140 (MQ=255)

                                                                                                                  aaTGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTGAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGACGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATg                  >  2:4401200/1‑140 (MQ=255)

                                                                                                                  aaTGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATg                  >  2:4717043/1‑140 (MQ=255)

                                                                                                                   aTGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                 >  1:2192936/1‑140 (MQ=255)

                                                                                                                    tGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                <  2:1449597/140‑1 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCTCAATATCAGGTGTCAAGAATGgag               >  2:2411357/1‑140 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGtag               >  2:3484797/1‑140 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag               <  1:2312488/140‑1 (MQ=255)

                                                                                                                        aGGGAATCCGTTCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt            >  2:12712/1‑140 (MQ=255)

                                                                                                                        aGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt            >  1:2808307/1‑140 (MQ=255)

                                                                                                                        aGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt            >  2:2486479/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc          >  1:1478726/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc          >  2:4121088/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGCtc          >  1:1691692/1‑140 (MQ=255)

                                                                                                                             aTCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg       <  1:3827271/140‑1 (MQ=255)

                                                                                                                              tCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATTCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGc      >  2:4268679/1‑140 (MQ=255)

                                                                                                                              tCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGc      >  1:1745246/1‑140 (MQ=255)

                                                                                                                               ccGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGct     <  2:1417826/140‑1 (MQ=255)

                                                                                                                                  tGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTCGAATAAACATCAGCTGTCTCATCATGCTGATGCAAAGTGAGCATTTCAGTCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGCGTTCTCGCtctc  >  2:2709338/1‑140 (MQ=255)

                                                                                                                                  tGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGACTAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc  >  1:1862699/1‑140 (MQ=255)

                                                                                                                                  tGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc  >  2:1925569/1‑140 (MQ=255)

||

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NZ_CP025268/1223956‑1224223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`