BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	92.9%	840.5 / 48.2	240	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (10/7); new base T (114/108); total (125/115)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NZ_CP025268/1374716‑1374987

  cGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑t‑t‑t                                                                                                                                       >  1:3031604/1‑140 (MQ=255)

  cGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑t‑t‑t                                                                                                                                       >  1:4941572/1‑140 (MQ=255)

       gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑t‑t‑t‑ttt‑t‑t                                                                                                                               >  1:927739/1‑140 (MQ=255)

        tCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑t‑t‑t‑ttt‑t‑tt                                                                                                                              <  1:1080837/140‑2 (MQ=255)

               aagcaaATGAGATCCTCAGCGAGATGGGTTATGCCCACGTCGAGAACGCCGGTGGCCTGAAAGGCATCGCAATCCCGAACGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATCCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTcag                                                                                                                        <  2:4222390/140‑2 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTcgc                                                                                                                        <  1:3358084/140‑3 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTcac                                                                                                                        <  1:4665449/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTcac                                                                                                                        <  1:3294186/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTcac                                                                                                                        <  1:1508201/140‑1 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCacac                                                                                                                      <  1:1607850/140‑1 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCacac                                                                                                                      >  2:673640/1‑140 (MQ=255)

                   aaaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCt                                                                                                                    <  1:304476/140‑1 (MQ=255)

                      agagATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTAt                                                                                                                 >  1:667910/1‑140 (MQ=255)

                      agagATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTAt                                                                                                                 >  1:3404272/1‑140 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  2:4955513/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  2:2144416/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  1:3475245/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  1:4740057/140‑1 (MQ=255)

                           gccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  1:2413032/139‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCa                                                                                                      >  2:4742830/1‑140 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCa                                                                                                      <  2:3167679/140‑1 (MQ=255)

                                     gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGa                                                                                                  <  1:3171308/140‑1 (MQ=255)

                                      aTGGTATATACCCACGTTGAGTACGCCGGTGGCCTGAAAGACAGCGCAAGGCCGAAGGTCAAAGGTTGAAAGAATTCACCATGAGCGGCGCTTAGGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTTTCACAGCTTATTTACATCCCCATAGat                                                                                                 >  2:4895420/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAATACATCGCAATGCCGAAGGGCAAAGGTTAAAAGAATGCACCATGAGCGGCGCTTATGCGC‑CG‑‑T‑T‑T‑TTT‑TGTCTGTCACACCTTATTTACTTTCCCGTTGat                                                                                                 >  2:4386476/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGTTT‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGa                                                                                                  >  2:3290217/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  1:389632/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  1:4279410/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑t‑t‑t‑ttt‑t‑tt                                                                                                                              >  2:548814/1‑109 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑t‑t‑t‑ttt‑t‑tt                                                                                                                              >  2:1938246/1‑109 (MQ=255)

                                           atatACCCACGTTGAGAACGCCGGTGGCCTGAAAGAAATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGTTT‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGattatt                                                                                             <  2:1523061/140‑1 (MQ=255)

                                              tgcccACGTTGAGAACGTCGGGTGCCTTAAAGACATCGCAATGCCGAAGGTTAAAGGTTAAAAGAATTCACCTTGAGCGGGGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTAGTTACATCCCCATAGATTATTTGCg                                                                                         <  2:1841278/138‑1 (MQ=255)

                                               cccccCGTTGAGACCGCCGGTGGCCTGCAAGACATCGCAATGCCCAAGGTCAAAGGTTAAAAGAATTAACCATGAGCGGCGCTTATGCTC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTAGTTGCGt                                                                                        <  2:4445863/139‑1 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAGGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑T‑T‑T‑TTTGT‑TGTGTCACAACTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  2:1198606/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTCTTTACATCCCCATACATTTTCTGCGt                                                                                        >  2:2585716/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:741175/140‑1 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:1955488/140‑1 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:102975/140‑1 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  2:2874911/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCATGAAAGACATCGCAATGCCGAAGGGCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑TCT‑T‑CTT‑T‑TCTGTGCCCCCCTATTTACCTCCCCCACGATCATTCGCGt                                                                                        >  2:3605159/1‑140 (MQ=255)

                                               aCCCACGTTGAAAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  2:355347/140‑1 (MQ=255)

                                                cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  1:331656/119‑1 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGTTT‑T‑T‑TTT‑T‑TCTGTCACAcc                                                                                                                     <  1:2963079/107‑1 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGTTT‑T‑T‑TTT‑T‑TCTGTCACAcc                                                                                                                     >  2:2963079/1‑107 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            >  1:2466003/1‑115 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGcg                                                                                   >  1:4515663/1‑139 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:1331663/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:2176472/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:2848636/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:5033876/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   <  1:172338/140‑1 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGGTAAAAGAATTCACCATGAGCGGCGCGTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:3181679/1‑140 (MQ=255)

                                                     gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGTTT‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:4078292/1‑140 (MQ=255)

                                                     gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTc                                                                                  <  2:3982863/140‑1 (MQ=255)

                                                     gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTc                                                                                  >  1:2292666/1‑140 (MQ=255)

                                                        gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATGCCCATAGATTATTTGCGTGAGCTcaca                                                                               >  1:2244571/1‑140 (MQ=255)

                                                         agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                              >  2:4523192/1‑140 (MQ=255)

                                                         agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                              >  1:3818887/1‑140 (MQ=255)

                                                          gaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaaa                                                                             >  1:4419600/1‑140 (MQ=255)

                                                              gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACg                                                                         >  1:472332/1‑140 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGc                                                                        >  1:484546/1‑140 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGc                                                                        >  1:2757406/1‑140 (MQ=255)

                                                                      ccTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc                                                                 <  1:4017748/140‑1 (MQ=255)

                                                                               cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                        >  1:2646822/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTTTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                       >  2:470707/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                       >  1:478953/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                       >  2:687267/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTGTTTCCCTGGTaaaaa                                                       >  2:2673013/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATCGACCATGAGCGGCGCTTATGCGC‑CG‑‑T‑T‑TGTTG‑T‑TCTGTCACACCTTGTTTACAGCCCCATAGATTATTTGCGTGAGCTTAACTATAGGCTTTTTCTTTGGTaaaaa                                                       >  2:4861288/1‑140 (MQ=255)

                                                                                 tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa                                                      >  1:1663125/1‑140 (MQ=255)

                                                                                 tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa                                                      >  1:1481707/1‑140 (MQ=255)

                                                                                    gaaTGCCGAAGGTCAAAGGTTAAAAGAATTCAAAATGAGCGGGGCTTATGCGG‑CGA‑T‑C‑T‑TTT‑A‑TGTGTCACACCTTATTTGCAACCCAATAGGTTATTGGTGAGAGCTCAGAAATACGCTTTTTCCCTGGTAAAAAATGa                                                   >  2:602783/2‑140 (MQ=255)

                                                                                      aTGCCGAAGGGCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑TGT‑T‑TTT‑T‑TCTGTCACACCTTAGTTACATCCGCATAGATTGTTTGCGTCAGCTCACAAATACGCTGTTTCCTTGGTAAAAAATGAtt                                                 >  2:4825357/1‑140 (MQ=255)

                                                                                         ccGAGGGGCAAAGGTTAAAAGAATTCAACATGAGGGGCGCTTATGCGCGGG‑‑T‑G‑T‑TTG‑T‑GGTGTTACGCCTTAATTACATCCCGAGGGATGATAGGCGTGGTCTGTCAAATACGCGTTTTGGGTGGGAAAAAATggatggg                                              >  2:4254732/1‑134 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACAATGAGCGGCGCTTATTCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGGTTATTTGCGTCATCTCACAAATACGCTTTTGCCCTGGTAAAAAATGATTTCCTGc                                           >  1:1212617/1‑140 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  1:688773/140‑1 (MQ=255)

                                                                                                  aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACt                                     >  2:719806/1‑140 (MQ=255)

                                                                                                      gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa                                 <  2:1081913/140‑1 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt                             >  1:2027340/1‑140 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt                             >  2:3922971/1‑140 (MQ=255)

                                                                                                           aaGAATTCATCATGATCGGTGCTTATGCCA‑CG‑‑T‑TAT‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGTTTATTCGCGTCAGCTCACAAGTACGCTTTTTCCCTGGTAAAAAATGGTTTCCTGCGTGGCTAAGACCCtt                            <  2:2904349/140‑1 (MQ=255)

                                                                                                                ttCACGAGGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTAATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAACAATGATTTCCTTCGTGACTAAAACCCTTGTGCt                       >  2:4039661/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGC‑CGTTT‑T‑T‑TTT‑T‑TGTGTCACACCTTATTTACACCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTACGTGACTAACACCCTTGTGc                        >  2:3518971/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  1:4297000/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  1:3933088/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  1:4602957/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  1:5021983/1‑140 (MQ=255)

                                                                                                                      aTGAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTg                 <  1:4458875/140‑1 (MQ=255)

                                                                                                                        gatcggcgCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTCTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGCTAAAAAATGATTTCCTGCGTGACTAACACCCTTGTGCTCAATTGAc               <  2:2292849/137‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc               <  2:3700467/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc               >  2:5016185/1‑140 (MQ=255)

                                                                                                                         aGCGGCGCTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACa              >  1:3650152/1‑140 (MQ=255)

                                                                                                                             gcgcTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt          >  1:5043937/1‑140 (MQ=255)

                                                                                                                              cgcTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTGCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTa         >  1:4247062/1‑140 (MQ=255)

                                                                                                                               gcTTATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt        >  2:238112/1‑140 (MQ=255)

                                                                                                                                  tATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt     <  2:2490963/140‑1 (MQ=255)

                                                                                                                                  tATGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt     <  1:3576410/140‑1 (MQ=255)

                                                                                                                                    tGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCt   <  1:2875050/140‑1 (MQ=255)

                                                                                                                                    tGCGC‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCt   <  1:2874900/140‑1 (MQ=255)

                                                                                                                                     tcgc‑CGT‑T‑T‑T‑TTT‑T‑TCGGTCACACCTTATTTCCATCCCCATAGCTTATTTGCGCCCGCTCACAACTGCGCATTTTCCGTGGTAAACAATGATTTCCTGCGTGACTACAGCCCTTGTGCTCAATTGACAGGTTATTTCCTg  <  2:1669532/139‑1 (MQ=255)

                                                                                                                                     gcgc‑CGT‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg  <  1:2166689/140‑1 (MQ=255)

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑CG‑‑T‑T‑T‑TTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NZ_CP025268/1374716‑1374987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`