BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	97.5%	778.4 / 9.8	239	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (1/5); new base C (113/118); total (114/125)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

cGAAAAAAGTCGGAGTGATCAGAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgag‑c                                                                                                                                         >  2:694233/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  2:4850091/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:840506/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:1709700/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:4090431/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:2356685/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:2275303/1‑140 (MQ=255)

  aaaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATAGCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑c                                                                                                                                      >  2:1376859/1‑140 (MQ=255)

  aaaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑c                                                                                                                                      >  1:1070077/1‑140 (MQ=255)

   aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑cc                                                                                                                                     <  1:1453338/140‑1 (MQ=255)

     aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑ccca                                                                                                                                   <  2:4330849/140‑3 (MQ=255)

        gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑cccagcc                                                                                                                                <  1:1806731/140‑6 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCg                                                                                                                              <  2:922762/140‑1 (MQ=255)

             aGTGATCACAAACAGAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAGATAGAATCCCTGTTGTCAGCTGACACTTCCTCGTCCCCTATTGGTAAAATGCATTTGTGTTCCTACGTGTGTCC‑GC‑AGCCCGcaa                                                                                                                           <  2:2275439/140‑1 (MQ=255)

             aGTGATCACAAAAAAAGGCTTATATTTGCGCTGTCAATGGTTGACAAAAGATGAAATAGAATACCTTTCGTCAGCTGACCCTTCCTCTTCGCTTATTGGTTAAATGGATTTCTGTTCCTACGTGCGCCC‑CC‑AGCCCGcaa                                                                                                                           <  2:1802731/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGcaaca                                                                                                                         <  1:1579509/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGcaaca                                                                                                                         <  1:2308512/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGcaaca                                                                                                                         <  2:3980735/140‑1 (MQ=255)

               ggATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGcaaca                                                                                                                         <  2:259966/139‑1 (MQ=255)

                 aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAAt                                                                                                                       >  1:1276379/1‑140 (MQ=255)

                   cacaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTAGTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGa                                                                                                                     >  1:2464349/1‑140 (MQ=255)

                    acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATCTATGTCCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGAt                                                                                                                    <  1:4684141/140‑1 (MQ=255)

                      aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCa                                                                                                                  <  1:3584031/140‑1 (MQ=255)

                       aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCaa                                                                                                                 >  2:162125/1‑140 (MQ=255)

                           aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATg                                                                                                             >  1:3214778/1‑140 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCa                                                                                                        >  1:4285165/1‑140 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCa                                                                                                        >  2:3027896/1‑140 (MQ=255)

                                   tatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAg                                                                                                     <  2:1655737/140‑1 (MQ=255)

                                       tGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCa                                                                                                 <  2:4889279/140‑1 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  1:2694004/1‑140 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  1:1419528/1‑140 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCGGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATTCCGCAAAGAGCaa                                                                                                >  1:1163514/1‑140 (MQ=255)

                                         cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                               >  1:3742985/1‑140 (MQ=255)

                                            tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                            <  2:4061970/140‑1 (MQ=255)

                                            tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                            <  2:3035493/140‑1 (MQ=255)

                                            tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                            <  1:3161226/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  1:4897168/1‑140 (MQ=255)

                                                 aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  1:2022189/140‑1 (MQ=255)

                                                      tGACAAAAGATGAGATAGAATACCTTTTGTCAGCTGACACTTCCTCTTAGCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:3390336/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:2514673/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:3320447/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:3149650/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:618692/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:2038838/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:5046508/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:4830719/1‑140 (MQ=255)

                                                              gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                          <  1:979775/140‑1 (MQ=255)

                                                                 gAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAag                                                                       >  1:2607108/1‑140 (MQ=255)

                                                                    aTAGAATACGTCTTGTCAGCTGTCACTTCCTCTTATCTTATTGATAAAATGTATTTATGTAACTACGTCCG‑CC‑CCAAGCCCTCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                    <  2:2379999/140‑1 (MQ=255)

                                                                        aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                                >  2:296273/1‑140 (MQ=255)

                                                                           aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             <  2:138438/140‑1 (MQ=255)

                                                                            ccTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCa                                                            <  2:3349108/140‑1 (MQ=255)

                                                                             cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                           <  2:1250057/140‑1 (MQ=255)

                                                                             cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGGTTCAc                                                           >  2:1106244/1‑140 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAACGCGCAATCAACGCACCCGTCCAGTCACAAAGAGTCAGTTTCACg                                                          >  2:3856594/1‑140 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑CCACC‑AGCCCGCAACAATGATCAACATGCAGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:1332056/140‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  1:5107649/1‑140 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         <  2:1977042/140‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  2:3008137/1‑140 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTCATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATACAGAGTCAGTTTCACGc                                                         >  1:197366/1‑140 (MQ=255)

                                                                                ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                        >  1:4170582/1‑140 (MQ=255)

                                                                                ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                        <  2:5107649/140‑1 (MQ=255)

                                                                                ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                        >  1:1764247/1‑140 (MQ=255)

                                                                                  gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                      >  2:2477461/1‑140 (MQ=255)

                                                                                     aGCTGACCCTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCca                                                   <  2:118395/140‑1 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  2:3070122/1‑140 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  2:2122716/1‑140 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  2:821666/140‑1 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  1:297725/1‑140 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  1:856056/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:2525432/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:1538718/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTACTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGGCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:2694262/140‑1 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCACCCATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTGATAAATAGTCAGTTGCACGCAATCCACAACGCGCAGcc                                       >  2:1787837/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:2248825/1‑140 (MQ=255)

                                                                                                       tcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCa                                 <  2:2208055/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  1:5094224/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  2:3210411/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGACAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              >  2:3694023/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTGCGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCACTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:4577111/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCACCAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCCGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:2673393/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:5086524/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:4996624/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  2:3548558/1‑140 (MQ=255)

                                                                                                               aTAAAATGGATTTCTGTACCTACGTGCGACC‑CC‑CGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  2:4387344/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:302574/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  2:2940983/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:3218417/140‑1 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       >  1:707155/1‑140 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTACAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCAACATCAACGCCGTGCa                       >  1:2839806/1‑140 (MQ=255)

                                                                                                                     tGGATTTATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACa                   >  1:3104694/1‑140 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAACGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:4030900/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:1006049/140‑1 (MQ=255)

                                                                                                                             tGTTCCTACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCAAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcc           <  1:980917/140‑1 (MQ=255)

                                                                                                                                 cctACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             >  1:3447631/3‑134 (MQ=255)

                                                                                                                                 cctACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  2:3447631/132‑1 (MQ=255)

                                                                                                                                 cctACGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  2:869851/3‑140 (MQ=255)

                                                                                                                                     cGTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  1:1243160/140‑1 (MQ=255)

                                                                                                                                      gTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGc  >  1:3863910/1‑140 (MQ=255)

                                                                                                                                      gTGCGCCC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGc  <  1:4591136/140‑1 (MQ=255)

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CC‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`