BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,826,683	(A)_7→8	intergenic (‑247/+51)	chbB ← / ← osmE	PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,826,676	1	.	A	96.7%	1442.0 / 33.0	396	intergenic (‑240/+58)	chbB/osmE	PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE
Reads supporting (aligned to +/- strand): ref base . (10/3); new base A (262/121); total (272/124)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 398 total aligned reads displayed.

GCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826541‑1826810

           tataCGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGgcgc                                                                                                                          >  2:3526969/1‑140 (MQ=255)

                 gTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTAGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGc                                                                                                                    <  1:3523452/140‑1 (MQ=255)

                  tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGcc                                                                                                                   >  1:3236909/1‑140 (MQ=255)

                  tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGcc                                                                                                                   >  1:2932253/1‑140 (MQ=255)

                  tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGcc                                                                                                                   >  1:2772691/1‑140 (MQ=255)

                   ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCg                                                                                                                  >  2:2600827/1‑140 (MQ=255)

                     aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  1:2857550/1‑140 (MQ=255)

                     aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  2:1321945/1‑140 (MQ=255)

                       aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:1911879/1‑140 (MQ=255)

                       aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  2:166761/1‑140 (MQ=255)

                       aCCTGAGCAATAGTATTTCTTTGACCTTATAAATGGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  2:3010150/1‑140 (MQ=255)

                            aGCAATAGTCTTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTTGCGAAGATAATCAAGAACGCCGGCC‑AAA‑AAA‑ACCCGACGCAATGGCCGGTTTCCggt                                                                                                         >  2:3496524/1‑138 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCACATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:641934/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCACATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4966437/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:714958/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4022331/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4337274/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4573678/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4753844/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:5011111/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:1072705/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:5097589/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:563524/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:2712534/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:484235/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:480691/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4491442/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1113007/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4169175/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:3269628/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1903479/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:2941316/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2254891/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2455943/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2393392/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:113932/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:1521974/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2993904/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:1435289/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:1239031/1‑140 (MQ=255)

                            aGCAATAGTATTTCTTTGACCTTATAAAGTGTTTCTCCGTGACCTGTGGTTATGACCCTCGGCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CAGGCGCAATAGCCGGTTTCGgtt                                                                                                         >  2:2083129/1‑140 (MQ=255)

                                 tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  1:1855579/1‑140 (MQ=255)

                                 tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  1:1321411/1‑140 (MQ=255)

                                 tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTGCCGTTGTTAc                                                                                                    >  1:3108764/1‑140 (MQ=255)

                                 tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATGAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  1:3644204/1‑140 (MQ=255)

                                      tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  2:2139274/1‑140 (MQ=255)

                                      tttCTTTGACCCTATAAAGTGTTCCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATATTTCGAGAAGGGAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCAACGCATGGGCCGGTTTCAGCTGTTACTTAAg                                                                                               >  2:1751259/1‑140 (MQ=255)

                                        tCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             <  2:2765016/140‑1 (MQ=255)

                                            tGACCTTATAAATTGTTTCTCCTTGGCCTGTGGTTATGACGCTCTTCACATTTCGGGCAAATAATTCGCGAAGAGAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAAGGGCCGGTTTCCGTTGGTACTCAAGCTGTCa                                                                                         >  2:2580574/1‑140 (MQ=255)

                                              aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                       >  1:734985/1‑140 (MQ=255)

                                               ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  2:3431404/140‑1 (MQ=255)

                                                   ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                  >  1:4418442/1‑140 (MQ=255)

                                                          gTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTAc                                                                           >  2:239029/1‑140 (MQ=255)

                                                           tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACt                                                                          >  2:1374552/1‑140 (MQ=255)

                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  2:3390252/1‑140 (MQ=255)

                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:2345260/1‑140 (MQ=255)

                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  2:3923010/1‑140 (MQ=255)

                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:3827097/1‑140 (MQ=255)

                                                              ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:3693514/140‑1 (MQ=255)

                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:3838738/140‑1 (MQ=255)

                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:3448975/140‑1 (MQ=255)

                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:4189662/140‑1 (MQ=255)

                                                                    gACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCt                                                                 <  2:504214/140‑1 (MQ=255)

                                                                     aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:600689/140‑1 (MQ=255)

                                                                     aCCTGTGGTTATGACCCTCTTCACATTGCGGGAAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:564128/140‑1 (MQ=255)

                                                                         gtgGTTATGACCCTCTTCACATTTCTGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAA‑AAACA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGa                                                            <  2:2758786/140‑1 (MQ=255)

                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  1:484235/140‑1 (MQ=255)

                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTCTGGAt                                                           >  1:2695023/1‑140 (MQ=255)

                                                                           ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATc                                                          >  1:79497/1‑140 (MQ=255)

                                                                            gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa                                                         <  2:2830736/140‑1 (MQ=255)

                                                                            gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa                                                         <  2:796497/140‑1 (MQ=255)

                                                                               aggACCCTCTTCACATGTCGGGCAAAGAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTCTCAGACGAATTGATTACTTCGCAGCCTGTGGATGAggg                                                      >  2:4687871/3‑138 (MQ=255)

                                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  1:2502554/1‑140 (MQ=255)

                                                                                 gccccTCTTCACCTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGCTCAGTGTc                                                    <  2:3605813/138‑1 (MQ=255)

                                                                                 gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  2:4393461/140‑1 (MQ=255)

                                                                                 gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:90915/140‑1 (MQ=255)

                                                                                    ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  2:2795943/1‑140 (MQ=255)

                                                                                    ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:2077032/1‑140 (MQ=255)

                                                                                    ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:1004029/1‑140 (MQ=255)

                                                                                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  1:1754824/1‑135 (MQ=255)

                                                                                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               <  2:1457963/140‑1 (MQ=255)

                                                                                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTAAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               <  1:1979961/140‑1 (MQ=255)

                                                                                           acacTTCCGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCACTGGCCGGTTTCCGTTGCTACTCAAGCTTTCAGACGAATTGATTACCTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:1723592/140‑1 (MQ=255)

                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGTATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:3579612/140‑1 (MQ=255)

                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:3699935/140‑1 (MQ=255)

                                                                                           acaTTTCGGGAAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:2193622/140‑1 (MQ=255)

                                                                                            caTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcac                                         >  2:907878/1‑140 (MQ=255)

                                                                                            caTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcac                                         >  2:101979/1‑140 (MQ=255)

                                                                                               ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGGTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAgg                                      >  2:4026187/1‑140 (MQ=255)

                                                                                                tCGGGAAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCAGTTGTTACTCAAGCTTTCAGACGAATTGTTTACATCGCAGCCTGTGGTTTAGTGTCGTCTTCAGCACAGGt                                     >  2:5012122/1‑140 (MQ=255)

                                                                                                 cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc                                    >  2:4318981/1‑140 (MQ=255)

                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   >  1:3932860/1‑140 (MQ=255)

                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGCATTCAGCACAGGTCt                                   >  2:1599750/1‑140 (MQ=255)

                                                                                                                     aTAATTAAGAACTCAGGCC‑AAACAAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  2:2685235/140‑1 (MQ=255)

                                                                                                                         ttAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGt            >  1:4301149/1‑140 (MQ=255)

                                                                                                                          tAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:1243526/140‑1 (MQ=255)

                                                                                                                          tAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:2333175/140‑1 (MQ=255)

                                                                                                                          tAAGAACTCAGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:2864485/140‑1 (MQ=255)

                                                                                                                                   aGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCgt                                                                                                          >  2:3124114/1‑36 (MQ=255)

                                                                                                                                   aGGCCAAAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGCTGATGACATGTCCGGTatcat  >  2:3977942/1‑140 (MQ=255)

GCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAA‑AAA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826541‑1826810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`