BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	94.5%	962.3 / 40.1	274	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (10/5); new base T (158/100); total (169/105)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

AACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCA  >  NZ_CP025268/1873693‑1873965

    aCAGCGAACCGTGGGCCTGCGAAGCCGCCACACAGCCGTAGCGGACAGTGGGCCAATGGAGTACGGGGGGCATACTGGCTGGTTTATTGACCGGCGAGTGAACAGCAAAAACAAAAGAATGGTGCAGCGCCAGC‑ggattt                                                                                                                                      >  2:2216003/1‑135 (MQ=255)

    aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAACTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tg‑ttt                                                                                                                                    >  2:760788/1‑140 (MQ=255)

    aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGGTTATTGACCGGCGTCTGAACGGCAAAAACAAAAACATGGTGAATCGCGAGC‑G‑tt‑ttt                                                                                                                                    >  2:2889515/1‑140 (MQ=255)

     cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGGCAGC‑G‑tt‑tttt                                                                                                                                   >  2:2726622/1‑139 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tttta                                                                                                                                  <  1:472749/140‑3 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tttta                                                                                                                                  <  1:3560071/140‑3 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tttta                                                                                                                                  <  1:3300787/140‑3 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tttta                                                                                                                                  <  2:3317445/140‑3 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tttta                                                                                                                                  <  1:2337310/140‑3 (MQ=255)

        cataCCGTGGGCCTGAGAAGCGGCAACACAGGTGTAGCATACAGTTGGCGAATGTAGTGCGGAGGGCATATGCACTGGGCTATTGTGCGGCGTCTGACCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGG‑TA‑TTT‑ACg                                                                                                                                <  2:3692465/137‑1 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATGTCCAGC‑G‑tt‑ttttacg                                                                                                                                >  1:2957120/1‑136 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑ttttacg                                                                                                                                <  2:3815386/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑ttttacg                                                                                                                                <  2:3121981/140‑5 (MQ=255)

          aaCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGcc                                                                                                                              >  1:5102900/1‑140 (MQ=255)

          aaCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGGAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGcc                                                                                                                              >  2:1728760/1‑140 (MQ=255)

             cGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TTT‑ACGCCGTta                                                                                                                          >  1:2175564/1‑140 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTtat                                                                                                                         >  1:2125162/1‑140 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTtat                                                                                                                         >  1:4873299/1‑140 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTtat                                                                                                                         >  1:418375/1‑140 (MQ=255)

                gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTGTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTtata                                                                                                                        >  1:1223664/1‑140 (MQ=255)

                       agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCa                                                                                                                 >  2:583637/1‑140 (MQ=255)

                           gCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAAtt                                                                                                             >  2:4855124/1‑140 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTa                                                                                                            >  2:1032620/1‑140 (MQ=255)

                              gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTaaa                                                                                                          <  2:1085504/140‑1 (MQ=255)

                                 acacAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGGCCGGCGTCTGAACGGCAAAACCAAAAGCATGGTGCATCGCCCGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTACACAg                                                                                                       >  1:1632918/1‑140 (MQ=255)

                                 acacAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAg                                                                                                       >  1:381627/1‑140 (MQ=255)

                                   acaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                                     <  1:3126819/140‑1 (MQ=255)

                                       gCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGAtc                                                                                                 >  1:195423/1‑140 (MQ=255)

                                          tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                              <  1:1429842/140‑1 (MQ=255)

                                              aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:145681/140‑1 (MQ=255)

                                              aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  2:725672/140‑1 (MQ=255)

                                              aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:75262/140‑1 (MQ=255)

                                              aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:2602621/140‑1 (MQ=255)

                                               tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                         >  2:132105/1‑140 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                       >  2:3173834/1‑140 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                       >  1:4704120/1‑140 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                       >  2:2403423/1‑140 (MQ=255)

                                                    ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGTGCAAATTAAACAGTCGATCTCCGAGGCCATta                                                                                    >  2:1219846/1‑140 (MQ=255)

                                                     tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                                   <  2:5020601/140‑1 (MQ=255)

                                                      ggCAAATGTCGTACGGGGGGCATATGACCTGGTTTATTGACCGGCTTCTGAACGTCAAAAACCAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                  >  2:638763/1‑140 (MQ=255)

                                                      ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                  >  1:4039984/1‑140 (MQ=255)

                                                      ggCAAATGTAGTACGGGGGGCATATGACATGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                  >  2:4074145/1‑140 (MQ=255)

                                                      ggCAAATGTAGTACGGGGGGCAGATTACCTGGTGTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TTTGACGCCGTTATATAGCGCAAATTCAACAGTCGATCTCCGAGGCCATtaat                                                                                  >  2:1525872/1‑140 (MQ=255)

                                                       gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                 >  1:1095108/1‑140 (MQ=255)

                                                       gCAAATGGAGTACGGGGGGCATATGACCTGGTTTATTGACAGGCGTCTGAACGGCGCAAACAAAAGCATGGTGAATCGCCAGC‑G‑TTATTT‑ACGCAGTTATAAAGCGCAAATGAAACAGACGATCTCCGAGGCCAGtaata                                                                                 >  2:2341935/1‑140 (MQ=255)

                                                        gaaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGG‑TT‑TTT‑ACGCCGTTATAAAGCGCAAATTAGACAGTCGATCTCCGAGGCCATtaataa                                                                                <  2:3713214/139‑1 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAGAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                            >  2:1894868/1‑140 (MQ=255)

                                                              tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                          >  1:4622801/1‑140 (MQ=255)

                                                                 taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                       <  2:4099052/140‑1 (MQ=255)

                                                                   cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCCGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCCAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                     <  1:4896005/140‑1 (MQ=255)

                                                                   cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                     <  2:4396039/140‑1 (MQ=255)

                                                                   cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                     <  1:2735820/140‑1 (MQ=255)

                                                                     gggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCACGGTGGATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTGCGAGGCCAGTAATAAGCGTTCGGtgact                                                                   >  2:4221722/1‑140 (MQ=255)

                                                                      ggggCATATGACCTGGTTTATTGAGCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  2:3905092/1‑140 (MQ=255)

                                                                      ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  1:3282763/1‑140 (MQ=255)

                                                                      ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  1:2218379/1‑140 (MQ=255)

                                                                        ggactatGACCTGGCTAATTGACCGGCGTATGAACAGCAAAAACAAAAGCATGGTGAACCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAACCAGTCGATCTCCGCGGCCATCAAGAACCGCTCGGtggccgac                                                                <  2:4129218/136‑1 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                                >  1:589060/1‑140 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                                >  1:856111/1‑140 (MQ=255)

                                                                          cATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGt                                                              <  1:703473/140‑1 (MQ=255)

                                                                           atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                             >  2:3142232/1‑140 (MQ=255)

                                                                           atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                             >  2:2140014/1‑140 (MQ=255)

                                                                                  cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcg                                                      >  1:2667847/1‑140 (MQ=255)

                                                                                    ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                    >  2:259368/1‑140 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGG‑TT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   >  1:1422040/1‑140 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   >  1:2998560/1‑140 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   <  2:1491503/140‑1 (MQ=255)

                                                                                         aTTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                               <  2:3041130/140‑1 (MQ=255)

                                                                                          ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                              <  1:1388288/140‑1 (MQ=255)

                                                                                          ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                              >  2:2753282/1‑140 (MQ=255)

                                                                                          ttGACCGGCGTCTGAAAGCCAGACCCATCAGCATCGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGCGACAACGCGCCAATTAAGCAGTCGAGCTCCGAGGCCATGAATAAGCGGCCCGTGACTGACCTCGACAGCCGCGAATcc                                              >  2:2492191/1‑140 (MQ=255)

                                                                                            gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGt                                            >  1:1014915/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:2289450/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:1905284/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:934107/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  2:428532/1‑140 (MQ=255)

                                                                                                    tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                    >  1:4821543/1‑140 (MQ=255)

                                                                                                    tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                    >  1:4835131/1‑140 (MQ=255)

                                                                                                         aCGGCAAAAACAAAAGCATGGTGCATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                               <  1:3921143/140‑1 (MQ=255)

                                                                                                         aCGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                               <  2:1932809/140‑1 (MQ=255)

                                                                                                           ggCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             <  2:1650796/140‑1 (MQ=255)

                                                                                                           ggCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             >  2:1024338/1‑140 (MQ=255)

                                                                                                            gCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                            >  2:5077595/1‑140 (MQ=255)

                                                                                                             cAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                           >  2:2994053/1‑140 (MQ=255)

                                                                                                              aaaaaCAAAAGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                          <  1:4593501/140‑1 (MQ=255)

                                                                                                                     aaaGCATGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGAAAATTAAACCGCCTATCTCCGCGGCCATTGATAAGCGTTCGCTGGCCGACGTCGACAGCGGCGAATCCGTATCCATTCTCACGGAAGATATTAGc                   <  2:198003/140‑1 (MQ=255)

                                                                                                                          aTGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCTGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc              <  1:2827268/140‑1 (MQ=255)

                                                                                                                          aTGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc              <  1:3897117/140‑1 (MQ=255)

                                                                                                                           tGGTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACATTCGATCTCCTAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACATCTGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcct             >  1:288888/1‑139 (MQ=255)

                                                                                                                            ggTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa            >  1:4926429/1‑140 (MQ=255)

                                                                                                                             gTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           <  2:4742607/140‑1 (MQ=255)

                                                                                                                             gTGAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           <  1:1768735/140‑1 (MQ=255)

                                                                                                                               gAATCGCCGGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAGTCCCACGGAAGATATTAGCGAACCGATGt         <  2:3541208/140‑1 (MQ=255)

                                                                                                                               gAATCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt         <  2:4919037/140‑1 (MQ=255)

                                                                                                                               gAATCCCAAGC‑GTTT‑TTT‑ACCCCGTTATAAAGCGCAAATTTATAAGACGGTCTCGGAGGCCATTAATAAGCGTTCGGAGACCTACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt         <  2:3404947/140‑1 (MQ=255)

                                                                                                                                 aTCGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGttt       <  1:808876/140‑1 (MQ=255)

                                                                                                                                   cGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtca     >  1:4107042/1‑140 (MQ=255)

                                                                                                                                   cGCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGCAAGATATGAGCGAACCGATGTTtca     >  1:876234/1‑140 (MQ=255)

                                                                                                                                    gCCAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcat    <  1:427252/140‑1 (MQ=255)

                                                                                                                                      cAGC‑GTTT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatca  <  1:1984622/140‑1 (MQ=255)

AACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TTT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCA  >  NZ_CP025268/1873693‑1873965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`