BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	98.3%	1050.2 / 7.0	289	coding (88/144 nt)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (3/2); new base T (165/119); total (168/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑G‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGATGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAACAATTGTACATCCT‑gta                                                                                                                                      >  2:3276756/1‑138 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑gta                                                                                                                                      >  1:2747008/1‑138 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑gta                                                                                                                                      >  1:5029103/1‑138 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑gta                                                                                                                                      >  2:543689/1‑138 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑gta                                                                                                                                      >  1:1979875/1‑138 (MQ=255)

    ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑gtatc                                                                                                                                    <  2:240127/140‑5 (MQ=255)

    ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑gtatc                                                                                                                                    >  1:1250029/1‑136 (MQ=255)

         ttCACCACCTCTGAGTCGTGGCGCTATTCTACCACTGCTGGAAAGGATGAAAATCTGGTGCTGAAAAGATGATATCACCACGGGTTAAACTGGTTAATGGCACAAATGTCGCTGAAAAATATTACATCCT‑GTATCGcaca                                                                                                                               >  2:1601812/1‑140 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGcaca                                                                                                                               >  1:4395918/1‑140 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGcaca                                                                                                                               <  2:195123/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCAcatc                                                                                                                             <  1:5054512/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCAcatc                                                                                                                             <  2:2607877/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCAcatc                                                                                                                             <  2:2617106/140‑1 (MQ=255)

              caccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATg                                                                                                                          <  1:1003841/140‑1 (MQ=255)

                   ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTTGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGaa                                                                                                                     <  2:423228/140‑1 (MQ=255)

                   ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGaa                                                                                                                     >  2:3798674/1‑140 (MQ=255)

                   ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGaa                                                                                                                     >  1:3515293/1‑140 (MQ=255)

                   ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGaa                                                                                                                     <  1:2573622/140‑1 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCt                                                                                                                <  2:4525791/140‑1 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCt                                                                                                                <  1:2397806/140‑1 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCt                                                                                                                <  2:622316/140‑1 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGCAGCGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCt                                                                                                                <  2:3374363/140‑1 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGCGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:1140037/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:2351658/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:2540741/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:4820184/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:2896535/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATAATGTTGAATACCTgcg                                                                                                             >  2:1986039/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  1:4138385/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCGAGTGCTGAAAAAAGGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCGGAAAAATTGTACATCCT‑GTATCGCACAGCATGTTGAATACCTGCGcc                                                                                                           >  1:1952279/1‑140 (MQ=255)

                              cgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGccc                                                                                                          >  1:971716/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:567931/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:2114853/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:1089629/1‑140 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaa                                                                                                >  1:3040945/1‑140 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaa                                                                                                >  2:3124257/1‑140 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:1740583/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACAACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:1326336/140‑1 (MQ=255)

                                          aCTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagc                                                                                              >  1:4964380/1‑140 (MQ=255)

                                           ctgctgGAGAGGCAGAAAATCTAGTGCTGAAACAATGAGATCACCACTCGATATACTGGTTAATGGCACAAATTTGGCTGAAAAACTGTACATCCT‑GTATCGCACATCATGTTGAATACTTGCGGCCAAAGCagcaacaa                                                                                             >  2:4861061/1‑137 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:2935114/1‑140 (MQ=255)

                                             gctgGAGAGGACGAAAATCTGGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAATTTTCGCGGAAAAATTGTACATGCT‑GTATCGCACATAATGTTGAATACCTGCGCCCAAAGCAGcaagtaag                                                                                           >  2:4764126/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTCGTGCGTAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCGAAAGCAGcaagcaag                                                                                           >  1:2142004/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATGCT‑GTATCGCACATCATGTCGAATACCTGCCCCCAAAGCAGcaagcaag                                                                                           >  2:3761647/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:3849642/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:2036622/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:1819933/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:3970868/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGAAGcaagcaag                                                                                           >  1:4898897/1‑140 (MQ=255)

                                             gctgAAGAGGAAGAAAATCTAGTCCTGAAAAAATGACATCACCACGGGATAAACATGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTCT‑ATCGCACACCATGATGAATACCTCCGCCCACAGAAAcaagcaag                                                                                           >  2:4404651/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:3914555/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  2:2179782/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  2:4034459/1‑140 (MQ=255)

                                                   gagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                                     >  1:85438/1‑140 (MQ=255)

                                                   gagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                                     >  2:3929670/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaccaccc                                                                                  >  1:3203026/1‑139 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  2:1533809/1‑140 (MQ=255)

                                                       aagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcaccac                                                                                 <  2:3851637/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGTTATCACCACTGCATAAACTGGTTAATAGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGACAAACCCACg                                                                                <  2:4197428/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  1:3104598/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  1:3230213/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  1:3941889/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  1:3865994/140‑1 (MQ=255)

                                                           aaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACa                                                                             <  2:1331158/140‑1 (MQ=255)

                                                           aaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACa                                                                             <  1:408614/140‑1 (MQ=255)

                                                                     cTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGa                                                                   <  1:2228229/140‑1 (MQ=255)

                                                                      tGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAc                                                                  <  2:1766635/140‑1 (MQ=255)

                                                                             aTGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGa                                                           >  1:2693636/1‑140 (MQ=255)

                                                                                  atCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCCAACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGAAAGCCAACACCACGACAACCAGAACGACCCATGGAACCtt                                                      >  1:3729397/1‑140 (MQ=255)

                                                                                       cacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCa                                                 >  1:327537/1‑140 (MQ=255)

                                                                                            gATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCa                                            >  1:2636827/1‑140 (MQ=255)

                                                                                             aTAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCaa                                           >  1:3451599/1‑140 (MQ=255)

                                                                                              tAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAt                                          <  1:4485131/140‑1 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  1:4646334/1‑140 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:891675/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:4646334/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:3003636/140‑1 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:3560128/1‑140 (MQ=255)

                                                                                                        aaTGGCACAAATTCCGCTGAAAAATTCGACATCCG‑GTATCGAACATCATGTTGAATACCTGCGCCCAAAGCTGTAAGCCAGCTAACACCTCGACTACCAGAACGACCCATCGAAATTTTTTCACTCCAATCTCCGGTTCa                                <  2:3515535/140‑1 (MQ=255)

                                                                                                         aTGGCAGAAATTCCCCTGAAAAATTGTACATCCTGG‑ATCGCTCATCATGTTGAAGCCCTGCGCCCAAAGCAGCAAGCACGCGAACACCGCGACAACCAGAACGACCCATCGAAACTTGTTCACTCCAATCTCCGTTTCac                               <  2:2701147/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATCCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACACCCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:2333241/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGCCCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:729424/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:2117730/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:1693802/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:4820207/140‑1 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAATGTGTACAGTCT‑GTATCGCAAATCATGTTGAATAACTGCGCCCAAAGCAACAAGCAAGACAACACCACGACAACCAGAACGACCCATCGAAACTGTTACACGCCAATCTCCGTTTCacc                              >  2:1167623/1‑140 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:2909995/1‑140 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTGCacc                              >  2:2749151/1‑140 (MQ=255)

                                                                                                                 aaTTCCGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTa                       <  2:27886/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCCT‑GTATCGCACCTCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  2:1000067/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:3014740/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:1836477/140‑1 (MQ=255)

                                                                                                                           aaaaaTCGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTaaa             <  2:1345912/140‑1 (MQ=255)

                                                                                                                               aTTGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAg         <  1:4434958/140‑1 (MQ=255)

                                                                                                                                ttGTACATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGc        <  1:2474011/140‑1 (MQ=255)

                                                                                                                                    aCATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  1:1166906/140‑1 (MQ=255)

                                                                                                                                    aCATCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  1:285051/140‑1 (MQ=255)

                                                                                                                                      aTCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:1431602/140‑1 (MQ=255)

                                                                                                                                      aTCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:4552436/140‑1 (MQ=255)

                                                                                                                                      aTCCT‑GTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  2:4096183/140‑1 (MQ=255)

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCT‑G‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`