Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,204,490 (A)7→8 pseudogene (175/3632 nt) CXP41_RS11705 → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,204,4831.A94.9% 916.5 / 34.5 253pseudogene (168/3632 nt)CXP41_RS11705DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (6/7);  new base A (98/142);  total (104/149)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

ATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCGCA  >  NZ_CP025268/2204344‑2204619
                                                                                                                                            |                                                                                                                                              
  aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaag‑‑aa                                                                                                                                             >  2:4434633/1‑140 (MQ=255)
  aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaag‑‑aa                                                                                                                                             >  1:5005158/1‑140 (MQ=255)
  aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaag‑‑aa                                                                                                                                             >  1:5005041/1‑140 (MQ=255)
  aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaag‑‑aa                                                                                                                                             >  1:3746331/1‑140 (MQ=255)
  aaCACCCAACTAGAACTTAAATATAAAAAACGCAAAACCCCCCTCAGCCATCGTCGCTGGCCGGGCGAACCAGTGCCCGTTATCACTGGAAGTCTCATCCAGACATTGGTTGATGAATTGCTACAAAAAGCTGAgaag‑‑aa                                                                                                                                             <  2:4466220/140‑1 (MQ=255)
         aaCTGGAACGGAAATATAAAACAGGCAAAACGGCGCTCATTCATCGTCGCTGGCCTCTGAAACGAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATGGCTACAAAAAGCTGAGAAG‑‑aaa‑a‑a‑a‑aac                                                                                                                                  <  2:2145823/140‑3 (MQ=255)
          aCTGCAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑aaa‑a‑a‑a‑aaca                                                                                                                                 <  2:1487608/140‑4 (MQ=255)
               aaCTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTc                                                                                                                            >  2:1726017/1‑140 (MQ=255)
               aaCTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTc                                                                                                                            >  2:2474769/1‑140 (MQ=255)
                     aaTATAAAAAAGGCAAAACTCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGt                                                                                                                      >  1:1727018/1‑140 (MQ=255)
                      atatAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGtt                                                                                                                     >  1:3837618/1‑140 (MQ=255)
                        ataAAAAAGCCAAATCGCCGCTCAGTCATCGTTGCTGGCCGGGCGAACCAGTGTCCGTTATCACTATAAGTCTCATCCAGACATTTGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTAt                                                                                                                   <  2:4992114/140‑1 (MQ=255)
                         taAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATg                                                                                                                  >  1:4103797/1‑140 (MQ=255)
                          aaaaaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATga                                                                                                                 <  2:1767943/140‑1 (MQ=255)
                              aaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAAt                                                                                                             >  2:3006999/1‑140 (MQ=255)
                              aaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAAt                                                                                                             >  1:1797349/1‑140 (MQ=255)
                                   aaaaCCCCGCTCAGTCATCGTCGCTCGCCAGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACCAAAACCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTc                                                                                                        <  2:530693/140‑1 (MQ=255)
                                     accgccgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAACCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCaa                                                                                                      <  2:3726325/138‑2 (MQ=255)
                                     aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                      <  1:5099424/140‑1 (MQ=255)
                                          cgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCAACCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAg                                                                                                 <  1:4831886/140‑1 (MQ=255)
                                           gcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGt                                                                                                >  2:1202514/1‑140 (MQ=255)
                                            cTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTg                                                                                               >  2:3520669/1‑140 (MQ=255)
                                             tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                              <  1:2116028/140‑1 (MQ=255)
                                             tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                              <  1:3777892/140‑1 (MQ=255)
                                             tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                              <  2:1370854/140‑1 (MQ=255)
                                               aGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCa                                                                                            <  2:3821300/140‑1 (MQ=255)
                                                gTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCa                                                                                            <  2:3110830/140‑1 (MQ=255)
                                                  cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                         <  2:1252669/140‑1 (MQ=255)
                                                  cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                         <  1:3282324/140‑1 (MQ=255)
                                                    tcgtcgCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATGGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTGTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCg                                                                                       >  2:3654885/1‑140 (MQ=255)
                                                         gCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATc                                                                                  >  1:900907/1‑140 (MQ=255)
                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATGGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTGTTCACTGGAGTGGCAGTCCGCCATCAc                                                                                >  2:701547/1‑140 (MQ=255)
                                                             gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                              <  2:4163583/140‑1 (MQ=255)
                                                             gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                              <  1:3156959/140‑1 (MQ=255)
                                                             gCCGGGCGAACCAGGGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTCTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                              <  2:2846415/140‑1 (MQ=255)
                                                              ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGCATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                             <  2:1198811/140‑1 (MQ=255)
                                                              ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                             <  1:4341598/140‑1 (MQ=255)
                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                         >  1:101429/1‑140 (MQ=255)
                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                         >  1:4597209/1‑140 (MQ=255)
                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                         <  1:2226098/140‑1 (MQ=255)
                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                         >  1:134761/1‑140 (MQ=255)
                                                                     aaCCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATc                                                                      <  1:4876816/140‑1 (MQ=255)
                                                                     aaCCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATc                                                                      <  2:4787292/140‑1 (MQ=255)
                                                                            gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                               <  1:3972374/140‑1 (MQ=255)
                                                                            gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑aaa‑a‑a‑a                                                                                                                                      <  2:3218289/70‑1 (MQ=255)
                                                                            gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑aaa‑a‑a‑a                                                                                                                                      >  1:3218289/1‑70 (MQ=255)
                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑AAA‑A‑A‑A‑AGCATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                           >  2:4537421/1‑140 (MQ=255)
                                                                                        tGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcaca                                                   <  2:250841/140‑1 (MQ=255)
                                                                                         ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                  <  1:2497349/140‑1 (MQ=255)
                                                                                         ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCAGCCATCACGCAGGCCATCCACTTGATCGGCGAACACaa                                                  >  1:1097349/1‑140 (MQ=255)
                                                                                               ctcATCCAGACAGTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                            >  2:1818806/1‑140 (MQ=255)
                                                                                                  aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                         >  1:1456908/1‑140 (MQ=255)
                                                                                                   tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                        <  2:4833540/140‑1 (MQ=255)
                                                                                                    ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTCTGAGCATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcc                                       >  1:2985043/1‑140 (MQ=255)
                                                                                                    ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcc                                       >  1:4006628/1‑140 (MQ=255)
                                                                                                        aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑AAA‑AGA‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGGAGTCCGCGATCACTCAGGCCATCAACTTGATCGGCTAACACAAACCCTCAATcccggc                                   <  2:835969/140‑1 (MQ=255)
                                                                                                         cATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcc                                  <  1:4744931/140‑1 (MQ=255)
                                                                                                            tGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACGCTCAATcccggcccgg                               >  1:2818976/1‑140 (MQ=255)
                                                                                                            tGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                               >  1:3931841/1‑140 (MQ=255)
                                                                                                            tGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                               >  1:214118/1‑140 (MQ=255)
                                                                                                            tGGGTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                               >  1:2409392/1‑140 (MQ=255)
                                                                                                             gggTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CAATGTATGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGa                              >  1:2524438/1‑140 (MQ=255)
                                                                                                              ggTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                             <  1:684530/140‑1 (MQ=255)
                                                                                                               gTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACa                            <  2:622287/140‑1 (MQ=255)
                                                                                                               gTGATGAATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACa                            <  2:620745/140‑1 (MQ=255)
                                                                                                                  atgaATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAGTg                         >  1:4237593/1‑140 (MQ=255)
                                                                                                                   tgaATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                        <  1:258417/140‑1 (MQ=255)
                                                                                                                   tgaATTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                        <  2:2364552/140‑1 (MQ=255)
                                                                                                                   tgaACTGCTACAAAAACCTGCGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAACTTTTCACTGGAGTGGCAGACCGCCATCAAGCAGGCCATCAACTTGATCGGCGAACACAACCCCTAAATCCCGGCCCGGACAATgg                        <  1:1328433/140‑1 (MQ=255)
                                                                                                                      aTTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggcgg                     >  2:2315644/1‑140 (MQ=255)
                                                                                                                      aTTGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGGCCGCCATCACGCAGGCCATCAACTTGATTGGCGAACACAAACCCTCAATCCCTGCCCGGACAATggcag                     >  2:1867197/1‑138 (MQ=255)
                                                                                                                        tGCTACAAGAAGCTGAGATGA‑AAA‑A‑A‑A‑A‑CAGTGTCTGGCGTTATGAGGATTTTTCACTGGAGTGGCAGTCCGCGATCACGCGGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:5060762/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CCTTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:4614636/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAGTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAATTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:3746331/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:4728931/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:759327/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:3541082/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:1079253/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:1769871/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:2099900/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:2633579/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:2731193/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:2929684/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:3483218/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:3254475/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:3789294/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  1:4434633/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:1938500/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAG‑‑AAA‑A‑ACA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:3381243/140‑1 (MQ=255)
                                                                                                                        tGCTACAAAAAGCTGAGAAG‑‑AAA‑A‑A‑AGA‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAGCTGGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   >  1:3049007/1‑140 (MQ=255)
                                                                                                                        tGCTACAAAAAGCAGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAACCTCAATCCCGGCCCGGAAAATGgcggcg                   <  2:1001444/140‑1 (MQ=255)
                                                                                                                        tGCGACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:1163250/140‑1 (MQ=255)
                                                                                                                        tGCCACAAAAAGCTGCGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATCAGAACTTCTCACTGGAGTGGCAGTCCGCCAACACGCAGGCCCCCAACTTGCTCCGCGACCACAACCCCTCCATCCCGGCCCGGACAATGgcggcg                   <  2:38899/140‑1 (MQ=255)
                                                                                                                         gCTAAAAAAAGCTGAAAAGAAAAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                   <  2:3904728/140‑1 (MQ=255)
                                                                                                                          cTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCt                 >  1:771925/1‑140 (MQ=255)
                                                                                                                          cTACAAAAAGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCt                 >  1:1703985/1‑140 (MQ=255)
                                                                                                                               aaaaGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATATTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt            >  2:4662247/1‑140 (MQ=255)
                                                                                                                                  aGCTGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTa         <  2:2207801/140‑1 (MQ=255)
                                                                                                                                     tGAGAAGA‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                        <  1:2199170/122‑1 (MQ=255)
                                                                                                                                         aag‑‑aaaga‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCGATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCGCa  <  2:2249351/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
ATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑‑AAA‑A‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCGCA  >  NZ_CP025268/2204344‑2204619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑