BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,466,923	(C)_8→9	intergenic (+242/‑123)	fadL → / → CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,466,915	1	.	C	94.8%	419.1 / 15.1	136	intergenic (+234/‑131)	fadL/CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (1/6); new base C (24/105); total (25/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑CC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCA  >  NZ_CP025268/2466777‑2467054

tttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCACAGCAATAAGCGGTAGCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑c                                                                                                                                               <  1:3964456/140‑1 (MQ=255)

 ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc                                                                                                                                              <  1:4329182/140‑1 (MQ=255)

  tGCAACCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAATCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCCTCCTTCTCAAAGAGTTTACTTTTCCGCATTTCCAGGA‑TA‑C‑T‑cc‑c                                                                                                                                            <  2:3574796/140‑1 (MQ=255)

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c                                                                                                                                            <  1:3795352/140‑1 (MQ=255)

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c                                                                                                                                            <  1:3320443/140‑1 (MQ=255)

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c                                                                                                                                            >  1:90160/1‑140 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCGATAAGCCGTATCCACAGCCAACCCTGAAGGGACAGGCATACTTTCAGGCGTTCTGCTCGTCCTTCTCAAAAAGTTTACTTTTCTGCCTTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  2:4218028/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATACGCGGCATCCACAGACAAACCTAAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  2:3143500/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  2:941060/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  1:4512351/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  1:215333/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  1:2065553/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  2:2185172/140‑1 (MQ=255)

      aaCAGCCTGTTGGAAGCTTGCCCTTTCAAATAAAAAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  1:4123924/140‑1 (MQ=255)

      aaCAGCCTGTTGACCGCTTGTCCTTTCAAAGCAATAAGCGGTATCCATAAAAAAACCTGCAGGGATATCCATATTTTCAGGCGTTCTTCTCGTCCTTCTTCAGGAATTTATTTTTCCGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  2:297361/140‑1 (MQ=255)

      aaCAGCCCGTTGGCAGCTTGCCCTCTCAAATCAATAACCGGTACCCACAGACAAACCTTAAGGGATAGGCATCTTTTCAGGCGTTCTGCTCGTCCTTCCCAAAGAGTTTACTTTTCTGCATTTCCACGA‑TA‑C‑T‑cc‑c‑cccc                                                                                                                                       <  2:237026/140‑1 (MQ=255)

        cAGCCTTTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccccc                                                                                                                                     <  2:1451950/140‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCAACTCAACAAGCGGTATCCACAGACAAACCTCAAGGGAAAGGCATATTTTCAGGCCTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TT‑C‑T‑cc‑c‑cccccc                                                                                                                                     <  2:734908/140‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccccc                                                                                                                                     <  1:4289618/140‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑cc‑c‑cccccc                                                                                                                                     <  2:734431/140‑2 (MQ=255)

            aggTTGGCAGCTTGCCCTTTTAAATCAATTAGCGGTATCTTCAGTCATACCTGAAGGGACAGGCATATTTTCAGGCGTTCTGCTCTTCCTTCTCAGAGTGTTTACTTTTCTGCATTTCCAGGA‑TG‑C‑T‑CCTC‑CCCCC‑TGGc                                                                                                                                 <  2:1558658/138‑1 (MQ=255)

              gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTa                                                                                                                               >  1:4890054/1‑140 (MQ=255)

              gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTa                                                                                                                               <  2:2953964/140‑1 (MQ=255)

                 ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtg                                                                                                                            <  1:1059974/140‑1 (MQ=255)

                 ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtg                                                                                                                            <  1:3311464/140‑1 (MQ=255)

                 ggCAGCTTGCCCTTTCAAATCAACAACCGGTACCCACAGACAAAACTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtg                                                                                                                            <  1:1059956/140‑1 (MQ=255)

                 ggCAGCTTGCCATTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtg                                                                                                                            <  2:2058989/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtgtg                                                                                                                          <  1:1309973/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtgtg                                                                                                                          <  1:4790977/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtgtg                                                                                                                          <  1:1846279/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATtgtg                                                                                                                          <  2:4787840/140‑1 (MQ=255)

                   cAGCTTCCCCCTTCAAACAAATAAGCGCTATCCACAAACAAACCCGAAGGAACAGTCATATTTTCAGGCGTTCTGCTCGTCCTCCTCAAAGAGTTTATCTTTATGCATTTCCCGGA‑TACC‑A‑CC‑C‑CCCCC‑TGGCTATtgtg                                                                                                                          <  2:817828/140‑1 (MQ=255)

                    aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTgc                                                                                                                         <  1:2874646/140‑1 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTgcg                                                                                                                        <  1:793675/140‑1 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTgcg                                                                                                                        >  1:57146/1‑140 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTgcg                                                                                                                        <  2:704283/140‑1 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTgcg                                                                                                                        <  2:1315741/140‑1 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTgcg                                                                                                                        <  1:3292216/140‑1 (MQ=255)

                         gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCa                                                                                                                    <  1:3770244/140‑1 (MQ=255)

                               tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                              <  1:1712639/140‑1 (MQ=255)

                                cgaaTCAAGAAGCGGTCTCCACAGACAAACCTGAAGGGAAAGGCCTATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  1:3110965/138‑1 (MQ=255)

                                cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  1:4670706/140‑1 (MQ=255)

                                   aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCaaa                                                                                                          <  1:4207829/140‑1 (MQ=255)

                                      aatactcGGTATCCACCTACAAACCTGAAGGGAAAGGCATATTTTAAGGCGCTCTGCTCGTCCTTCTCAAATAGTTTACTTTTCTGCATTTCCCGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTa                                                                                                       <  2:2650716/134‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  2:4236490/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  2:5068612/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  2:1022904/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  1:454912/140‑1 (MQ=255)

                                             cgTATCCACCGACAAACCTGAAGGGAAAGGCATATTCTCACGCGTTCTGCTCCCCCTTCTCCAAGCGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TCGCTATTATGCGCTCATACACTCAAATTAAAGATAg                                                                                                <  2:1384470/139‑1 (MQ=255)

                                                tttcacaGTCAAGCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTTCTTCTTAAAGAGTTTACTTTTCTGCATATCCAGGC‑TA‑CGC‑CC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  2:1966641/137‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAACGATAGGtt                                                                                             <  2:4381112/140‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  2:2441782/135‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  1:2036147/140‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGATCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             >  2:4510463/1‑140 (MQ=255)

                                                 tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTc                                                                                            >  1:137709/1‑140 (MQ=255)

                                                      aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                       >  2:1359867/1‑140 (MQ=255)

                                                       gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                                                                      >  2:1239227/1‑140 (MQ=255)

                                                       gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTGCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                                                                      >  2:4008552/1‑140 (MQ=255)

                                                                  aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                                           <  2:302905/140‑1 (MQ=255)

                                                                      aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                       >  2:3387095/1‑140 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGTGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  2:2939166/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      >  1:110046/1‑140 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  1:535825/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  1:4008552/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCAAACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  1:4348174/140‑1 (MQ=255)

                                                                         ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                                    <  1:4431988/140‑1 (MQ=255)

                                                                             tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                                                                >  1:1791128/1‑140 (MQ=255)

                                                                              tttttCAGGCGTTCTGCTCCTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGTA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                               <  1:4821605/139‑1 (MQ=255)

                                                                              atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                               <  2:1350960/140‑1 (MQ=255)

                                                                              atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                               <  1:180406/140‑1 (MQ=255)

                                                                                tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTTCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAtt                                                             >  2:3710647/1‑140 (MQ=255)

                                                                                   cacgCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAAGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                          <  2:4653367/137‑1 (MQ=255)

                                                                                   cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                          <  2:4980248/140‑1 (MQ=255)

                                                                                   cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                          <  1:1587290/140‑1 (MQ=255)

                                                                                    aGGCTTTCTCCTCGTCCTTCGCAAAGAGCTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                         <  2:4121022/140‑1 (MQ=255)

                                                                                    aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGTTAGTCTATTTCAt                                                         >  2:584144/1‑140 (MQ=255)

                                                                                     ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  1:1883015/140‑1 (MQ=255)

                                                                                     ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  1:3665684/140‑1 (MQ=255)

                                                                                     ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  1:1992990/140‑1 (MQ=255)

                                                                                     ggCGTTCTACTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAAGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  2:5089001/140‑1 (MQ=255)

                                                                                        gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                     <  1:3473098/140‑1 (MQ=255)

                                                                                        gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                     <  2:1371606/140‑1 (MQ=255)

                                                                                          tCTGCTCGTCCTTCTTAAAGATTTTACGTTTCTGAAGTTCCAGGATTC‑C‑C‑CC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                                   <  2:3807843/140‑1 (MQ=255)

                                                                                          tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGtc                                                   <  2:492128/140‑2 (MQ=255)

                                                                                          tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                                   <  2:492213/140‑1 (MQ=255)

                                                                                          tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                                   <  1:387731/140‑1 (MQ=255)

                                                                                              cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTTatat                                               <  2:4712488/140‑1 (MQ=255)

                                                                                                       ctcAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                                      <  1:2317051/140‑1 (MQ=255)

                                                                                                       ctcAAAGAGTTTACTTTTCTGCAGTTCCAGGG‑TA‑CTT‑CC‑C‑CCCCC‑TGGCTATGTTGAGCGGATACACTCAAATTAAAGGTAGGTTATAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATGTGTa                                      >  2:502310/1‑140 (MQ=255)

                                                                                                        tcAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTaa                                     <  1:2690001/140‑1 (MQ=255)

                                                                                                                tttACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaa                             >  2:4587143/1‑140 (MQ=255)

                                                                                                                 ttACTTTTCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                            <  1:5058439/140‑1 (MQ=255)

                                                                                                                      tttCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAAtcaatcaaga                       <  2:137586/140‑3 (MQ=255)

                                                                                                                      tttCTGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                       <  1:4602031/140‑1 (MQ=255)

                                                                                                                          tGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCACTCAAAATGGa                   >  2:1801939/1‑140 (MQ=255)

                                                                                                                          tGCATTTCCAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGa                   >  2:1114395/1‑140 (MQ=255)

                                                                                                                                  cAGGA‑TA‑C‑TCCC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATc           <  2:1790873/140‑1 (MQ=255)

                                                                                                                                        tA‑C‑T‑CC‑C‑CCCCCATGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCt      <  2:3241951/140‑1 (MQ=255)

                                                                                                                                         a‑C‑T‑CC‑CACCTCC‑TTGCTATTGTGCGCTCATACTCTCAAATTAAAGATAGCTTATAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTATGTAATATCTATTTGTAACAAAGCAATCAAAATGTAATAAAATCATGCTa     <  2:2748014/140‑1 (MQ=255)

                                                                                                                                             t‑CC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCa  >  1:3672022/1‑140 (MQ=255)

TTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TA‑C‑T‑CC‑C‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCA  >  NZ_CP025268/2466777‑2467054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`