BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	320,615	(G)_6→7	intergenic (+482/‑44)	rclR → / → ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	320,609	1	.	G	95.2%	599.6 / 22.3	191	intergenic (+476/‑50)	rclR/ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
Reads supporting (aligned to +/- strand): ref base . (9/0); new base G (90/90); total (99/92)
Fisher's exact test for biased strand distribution p-value = 3.49e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑t                                                                                                                                               >  1:2972767/1‑140 (MQ=255)

aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑t                                                                                                                                               >  2:4515482/1‑140 (MQ=255)

    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑ggg‑g                                                                                                                                       >  2:4992110/1‑140 (MQ=255)

     tGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑ggg‑gg                                                                                                                                      >  1:687041/1‑140 (MQ=255)

      gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑ggg‑gg‑g                                                                                                                                    >  1:570458/1‑140 (MQ=255)

            agaaagaaAATTAGTTATTGCTTTTGACTTTTGGATGAAAGATTATACTTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GC‑TG‑‑GGG‑GG‑GAAACa                                                                                                                               <  2:690694/140‑1 (MQ=255)

            agaaaTAAAATTAGTTATTGCATTTTACGTTTGGATGAAAGATGATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACa                                                                                                                               <  1:4653243/140‑1 (MQ=255)

              aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATc                                                                                                                             <  1:2373021/140‑1 (MQ=255)

              aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATc                                                                                                                             <  2:73202/140‑1 (MQ=255)

               aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCg                                                                                                                            <  1:1035878/140‑1 (MQ=255)

                 tAAAATTAGTTATTGCATTGGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCGAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCCGGG‑G‑‑‑GGG‑GG‑GAAACATCggg                                                                                                                          >  1:4291293/1‑140 (MQ=255)

                       tatttATTGCATTTTACGTTTGGATGAAAGATTATCATTTGTCATAAAAATGAGGGGTGGTATCTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGTTC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATaaa                                                                                                                   <  2:4842806/137‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCa                                                                                                             <  2:852141/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCa                                                                                                             <  1:2309053/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCa                                                                                                             <  1:815384/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCa                                                                                                             <  1:4052285/140‑1 (MQ=255)

                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATg                                                                                                           <  2:1829020/140‑1 (MQ=255)

                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CAGAAATGGGCATg                                                                                                           <  2:2244741/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTaa                                                                                                     <  2:3288382/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTaa                                                                                                     >  1:3766583/1‑139 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTaa                                                                                                     >  1:3766805/1‑139 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTaa                                                                                                     <  2:3766598/139‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAAt                                                                                                    <  2:877022/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATA‑TTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTaa                                                                                                     <  2:3765882/138‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATgg                                                                                                  <  1:3031087/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATgg                                                                                                  <  1:2980889/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATgg                                                                                                  <  2:1747382/140‑1 (MQ=255)

                                        gTTTGGATGAAAGAGTATCTTTTGTCATACACATGAGGGGTGGTATGTTGATAGCCACTTAAAAAAGAACGCCATATTTATCGATGATTGATC‑CCCA‑GG‑TA‑‑GGG‑GG‑GAAACATCGGG‑AATAAATGGGCATTAAGTAATgg                                                                                                  <  2:1137197/140‑1 (MQ=255)

                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGt                                                                                               >  1:4425311/1‑140 (MQ=255)

                                             gATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTAt                                                                                             >  2:590349/1‑140 (MQ=255)

                                                gAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CGCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAg                                                                                          <  2:2840809/140‑1 (MQ=255)

                                                     aTTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTtgtg                                                                                     <  2:2182678/140‑1 (MQ=255)

                                                          cATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGt                                                                                <  1:1211536/140‑1 (MQ=255)

                                                            tttGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCa                                                                              >  1:78603/1‑140 (MQ=255)

                                                              tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                                                                            >  2:300740/1‑140 (MQ=255)

                                                              tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                                                                            >  1:542384/1‑140 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                      <  1:1186842/140‑1 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                      <  2:1787530/140‑1 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                      <  2:2746373/140‑1 (MQ=255)

                                                                     cAAATGAGGGGGGGTCTGTTGCTAGCGAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CACC‑GG‑C‑‑‑GGG‑GGAGGAACATCGGG‑CCTAAATGGGCATGCAGTAAGGGAGTCTTGGCTGTGAATGTCACTATCttgg                                                                     >  2:3844389/1‑138 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                    <  1:1097622/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                   <  1:3829996/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                   <  2:44682/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                   <  2:4906250/140‑1 (MQ=255)

                                                                        aTGAGGGGTGGTAGGTTGCTAGCCAAATCAAACAGAACGCCATATTTATTGATGATTGATCTCCCG‑GG‑G‑‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCAGGAAGTAAGGGAGTATTAGTTGTGAATGTCAATTTCTTTTTC‑a                                                                 >  2:256518/1‑140 (MQ=255)

                                                                         tGAGGGGTGGTCTGTTGCTAGCAAATTAAAAAAGAACGCCATATTTATTTATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAGACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑Ac                                                                <  2:2852817/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑Ac                                                                <  2:3478366/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑Ac                                                                <  2:1050436/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑Ac                                                                <  2:2496352/140‑1 (MQ=255)

                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑Acc                                                               >  1:841048/1‑140 (MQ=255)

                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑Acc                                                               <  1:4585802/140‑1 (MQ=255)

                                                                          gAGGGGTGGGATGTTGCTGGCCAAGTAAAAAAGAAGGCCGTATGTATTGATGATTGGTC‑CCGC‑GG‑TG‑‑GGG‑GG‑GAAACATTGGG‑CATAAATGGGTATGAAGTAATGGAGTAGTAGTTGTGAATGTCAATTTAGTTGtgagc                                                                >  2:293560/1‑136 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTACTGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑CCCTGt                                                            >  2:2326131/1‑140 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGt                                                            >  1:2543497/1‑140 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGt                                                            >  2:4560081/1‑140 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CACC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATGTCTTTGTC‑ACCGGt                                                            >  2:1566004/1‑140 (MQ=255)

                                                                              ggtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAGATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTa                                                           <  1:3095776/140‑1 (MQ=255)

                                                                                tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTAtt                                                         >  1:2254037/1‑140 (MQ=255)

                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTTAATGTCAATTTCTTTGTC‑ACCTGTAGTgg                                                       >  2:2134321/1‑140 (MQ=255)

                                                                                    aTGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTg                                                     <  1:1482306/140‑1 (MQ=255)

                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACg                                                  >  1:4369216/1‑140 (MQ=255)

                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACg                                                  >  2:3655508/1‑140 (MQ=255)

                                                                                        tGCTAGCCCATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGGTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGc                                                 >  1:409240/1‑140 (MQ=255)

                                                                                        tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGATGc                                                 >  1:3923070/1‑140 (MQ=255)

                                                                                        tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGc                                                 >  1:1710954/1‑140 (MQ=255)

                                                                                        tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGc                                                 >  1:737048/1‑140 (MQ=255)

                                                                                          cTAGGCAATTAAAAAAGAACGCAATATTTATTGATGATTTATC‑CCTC‑GG‑TG‑‑GGG‑GG‑GAAACATAGGG‑CATAAATGGGCATGAAGTAATGGAGGATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGgcc                                               >  2:3677830/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGTGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGccc                                               >  2:1440500/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGGGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGccc                                               >  2:3992455/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACGTGTATTGGTGACGccc                                               >  2:4450127/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGccc                                               >  2:1043629/1‑140 (MQ=255)

                                                                                           tAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCt                                              <  1:1766264/140‑1 (MQ=255)

                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTg                                             >  2:4400436/1‑140 (MQ=255)

                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑GGGAGG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTg                                             >  1:3049181/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGa                                            >  1:3614317/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGa                                            >  1:1994208/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGAATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGa                                            >  2:3513321/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑GGG‑GG‑GGAAAAACGGGCCAAAAATGGGAAGGAAG‑AATGGGGGATTAGTTTGGAAAGTCAATTTCTTTGTCAACCTTTATTGGGGCGGCCCTGa                                            >  2:481323/1‑140 (MQ=255)

                                                                                               cAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TGGGGGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGa                                            >  1:902520/1‑140 (MQ=255)

                                                                                               cAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGA‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCGGaaa                                          >  2:552228/1‑140 (MQ=255)

                                                                                                 aTTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATc                                        >  1:4782441/1‑140 (MQ=255)

                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAACTCa                                       >  1:4123905/1‑140 (MQ=255)

                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:4145698/1‑140 (MQ=255)

                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:4760982/1‑140 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:51131/140‑1 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:1304568/140‑1 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:3300506/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATC‑CCCC‑GGAG‑‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑AGCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  2:3771847/1‑140 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  2:4972763/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  1:567980/140‑1 (MQ=255)

                                                                                                                cATATTTATTGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         >  2:3341335/1‑140 (MQ=255)

                                                                                                                  tatttattGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  1:3115678/140‑1 (MQ=255)

                                                                                                                     ttattGATGATTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCg                    >  1:2201990/1‑140 (MQ=255)

                                                                                                                         tgatgaTTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgct                >  2:3213179/1‑140 (MQ=255)

                                                                                                                         tgatgaTTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGAATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTTGTGACGCGCTGAAATCAAGAATGGCACGAGACTCCGtgct                >  2:458213/1‑140 (MQ=255)

                                                                                                                          gatgatTGATC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  2:4149754/1‑140 (MQ=255)

                                                                                                                            tgattgatC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTgctgct             >  1:1641578/1‑140 (MQ=255)

                                                                                                                              attgatC‑CCCC‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  2:3406766/1‑140 (MQ=255)

                                                                                                                                      cccc‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGaaaa    >  2:4311103/1‑140 (MQ=255)

                                                                                                                                        cc‑GG‑TG‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAc  >  1:4891929/1‑140 (MQ=255)

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATC‑CCCC‑GG‑T‑‑‑GGG‑GG‑GAAACATCGGG‑CATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTC‑ACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`