BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,509,139	+C	pseudogene (137/878 nt)	php ←	phosphotriesterase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,509,136	1	.	C	96.4%	1162.6 / 32.4	362	pseudogene (140/878 nt)	php	phosphotriesterase‑related protein
Reads supporting (aligned to +/- strand): ref base . (6/7); new base C (196/150); total (204/158)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

CACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑C‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAA  >  NZ_CP025268/3508998‑3509270

cacaTGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCATGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTacgcac‑g‑c                                                                                                                                          >  2:3951894/1‑140 (MQ=255)

cacaTGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTacgcac‑g‑c                                                                                                                                          <  1:1509953/140‑1 (MQ=255)

cacaTGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTacgcac‑g‑c                                                                                                                                          >  1:1598731/1‑140 (MQ=255)

  caccttCCGGGAAAAACGCGTCCTGGTCATCACCGTTACCGGCCACCACGTTGATCCCCGTCTCGCGCATTCCATCAAGCATCAATTGCGCCTTGCGCCCCCTGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑c‑c‑c                                                                                                                                      <  2:1565844/136‑1 (MQ=255)

  caTGTTCCGGGAAAAACGCGTCCTGCTAATAACCGGTACAGGCCACCCCGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATCGCGCATTGCGCCCCATGCAACGGTTGGTCATCTCACTCACATTACGCAC‑G‑c‑c‑c                                                                                                                                      <  2:3216811/140‑1 (MQ=255)

    tGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑c‑c‑ccg                                                                                                                                    <  1:1840187/140‑3 (MQ=255)

     gTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑c‑c‑ccgg                                                                                                                                   <  2:2104585/140‑4 (MQ=255)

       tCCGGGAAAAACGCGCCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑c‑c‑ccgggt                                                                                                                                 <  2:4068652/140‑6 (MQ=255)

                    cgTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTc                                                                                                                    <  2:4744419/140‑1 (MQ=255)

                     gTCCTGGTTATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATGAAGCATAAATTGCGCATTGCGCCCCATGTAACGGGTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCa                                                                                                                   <  2:3727151/140‑1 (MQ=255)

                                aaCCGGTACAGGCCACCACGTGGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCACCCCATGTAACGGTTGGTCATCTCAATCACATGACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaa                                                                                                        >  2:4228511/1‑140 (MQ=255)

                                 aCCGGTACAGTCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaaa                                                                                                       <  2:5020272/140‑1 (MQ=255)

                                 aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaaa                                                                                                       <  1:3091168/140‑1 (MQ=255)

                                 aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaaa                                                                                                       >  1:2449377/1‑140 (MQ=255)

                                 aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaaa                                                                                                       >  1:3330027/1‑140 (MQ=255)

                                 aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaaa                                                                                                       <  2:4776735/140‑1 (MQ=255)

                                 aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCaaa                                                                                                       >  1:1051267/1‑140 (MQ=255)

                                     gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATGACATTACGCAC‑GCC‑C‑CGGGT‑CCGCAGGGCGTTCAGCTCGTGGCAAATGaa                                                                                                   >  2:3903654/1‑140 (MQ=255)

                                     gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGaa                                                                                                   >  2:3312606/1‑140 (MQ=255)

                                     gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGaa                                                                                                   >  1:1078136/1‑140 (MQ=255)

                                      ttcAGGCCACGACGTTGATCCCCTTCTCGCGCATTACATCAAGCAGAAATTGCGCATTGCGCCCCATGTAATGGTTGGTCATCTCACTCACATTACGCAA‑GCC‑C‑CGGGA‑CATCAGGTCGCTCTTATACTGGCAAATCAAc                                                                                                  >  2:2384560/3‑140 (MQ=255)

                                      tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                  <  1:3000910/140‑1 (MQ=255)

                                      tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                  >  1:3239268/1‑140 (MQ=255)

                                      tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                  <  2:1391972/140‑1 (MQ=255)

                                      tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                  >  1:4038497/1‑140 (MQ=255)

                                           gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                             >  1:2777481/1‑140 (MQ=255)

                                           gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                             <  1:81319/140‑1 (MQ=255)

                                           gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                             <  2:1136253/140‑1 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAGATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  2:1613987/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  1:3956305/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  2:4878219/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  1:2206228/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        <  2:410741/140‑1 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  1:3790002/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  1:2301630/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  1:2870323/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                        >  2:2679922/1‑140 (MQ=255)

                                                cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACCGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCTGTCATCTCCTTGCAAATGAACGCATACTGAt                                                                                        >  1:899189/1‑140 (MQ=255)

                                                   gTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCaa                                                                                     <  2:1837110/140‑1 (MQ=255)

                                                     tGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAgg                                                                                   >  1:3128511/1‑140 (MQ=255)

                                                       aTCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcg                                                                                 >  1:3519721/1‑140 (MQ=255)

                                                        tCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcgg                                                                                <  2:2898887/140‑1 (MQ=255)

                                                        tCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcgg                                                                                <  1:1455315/140‑1 (MQ=255)

                                                          cccGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCa                                                                              >  1:4906887/1‑140 (MQ=255)

                                                                   cgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACgtt                                                                     >  1:4750474/1‑140 (MQ=255)

                                                                        tACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGtttt                                                                <  1:2686294/140‑1 (MQ=255)

                                                                           aTCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTaa                                                             <  1:1272179/140‑1 (MQ=255)

                                                                                gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCACGCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCg                                                        >  2:1881672/1‑140 (MQ=255)

                                                                                gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCg                                                        >  2:344654/1‑140 (MQ=255)

                                                                                gCATAAATTACGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCAGATTCCGCAC‑G‑C‑C‑TGGGGGCATCAGGTCGTTCATCTCCTGGAAAATGAAGTCATACTGATCAAGGCGGCAGTCCACGTTGGTTTTAAAGCCg                                                        >  2:4590206/1‑140 (MQ=255)

                                                                                  aTAAATTGCGCATTGCGCCCCATGTAACGGTTTGTCATCTCAATCACATTACGCACTG‑C‑C‑CGGGT‑CATCAGGTCGTTAATCTCCTGTCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTAAGTTTAAATCCAga                                                      >  2:3027088/1‑140 (MQ=255)

                                                                                  aTAAATTGCGCATTGCGCCCCATGTAACGGTTGGTAAGCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGCACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGga                                                      <  2:1690297/140‑1 (MQ=255)

                                                                                    aaaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgaga                                                    <  2:1854799/140‑1 (MQ=255)

                                                                                     aaTTGCGCATTGCGCCCCACGTAACGGCTGGTGATCTCAATCACATTACGCAC‑GCC‑C‑CGGAT‑CATCAGGGCGTTCATCTCCTGGCAAAAGAACGCATACTGATCAAGGCGGCAGTGCACGTTGTTTTTAAAGCGGgagag                                                   >  2:4460203/1‑140 (MQ=255)

                                                                                       ttgcgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGAt                                                 <  1:2046371/140‑1 (MQ=255)

                                                                                         gcgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCa                                               <  1:4103463/140‑1 (MQ=255)

                                                                                         gcgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCa                                               <  1:3294624/140‑1 (MQ=255)

                                                                                          cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGATCATCTCCTGGCAAACGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCaa                                              >  2:1939375/1‑140 (MQ=255)

                                                                                            cattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAata                                            <  1:5014612/140‑1 (MQ=255)

                                                                                            cagtgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑CTC‑CGGCT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGCCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAata                                            <  2:2749618/137‑1 (MQ=255)

                                                                                               tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGc                                         >  2:664652/1‑140 (MQ=255)

                                                                                               tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGc                                         >  1:2608049/1‑140 (MQ=255)

                                                                                                 cgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAg                                       <  1:2417940/140‑1 (MQ=255)

                                                                                                 cgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAg                                       <  1:1691434/140‑1 (MQ=255)

                                                                                                   ccccATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGAt                                     <  2:2870323/140‑1 (MQ=255)

                                                                                                        tGTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCa                                >  1:5058617/1‑140 (MQ=255)

                                                                                                         gTAACGGTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCAt                               <  2:709811/140‑1 (MQ=255)

                                                                                                              ggtgggtgATCGCAGTCACATTACGCAC‑G‑CTC‑CTGGT‑CATCAGGTCGTTCGTCTCCTGGCAATTGAACGCATACTGATCAAGGCGGCAGTCGACGTTGTTTTTAAAGCCGGAGGGATCAATATGCAGATGCTCATGGGCg                          <  2:3827364/140‑1 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          <  2:959794/140‑1 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          >  1:1543222/1‑140 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          <  1:4338386/140‑1 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          <  2:1987066/140‑1 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          <  1:1347737/140‑1 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          <  2:1191224/140‑1 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                          >  1:1289802/1‑140 (MQ=255)

                                                                                                              ggTTGGTCATCTCAATCACATTACGCAC‑G‑C‑CACGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCAGGAGAGATCAATATGCAGATGCTCATGGGCg                          <  1:1200158/140‑1 (MQ=255)

                                                                                                               gTTGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGa                         >  1:3416067/1‑140 (MQ=255)

                                                                                                                ttGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGTGCGAg                        >  1:4552589/1‑140 (MQ=255)

                                                                                                                ttGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg                        >  1:3638365/1‑140 (MQ=255)

                                                                                                                ttGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg                        >  2:3554605/1‑140 (MQ=255)

                                                                                                                ttGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg                        >  1:714722/1‑140 (MQ=255)

                                                                                                                ttGGTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg                        >  2:793005/1‑140 (MQ=255)

                                                                                                                ttGGTCAGCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg                        >  2:611276/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑TATCAGGTCGTTCATCTCCTTGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  1:542525/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCTGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  1:2818309/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  1:467049/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  1:4199475/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  1:4489368/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  1:4885007/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                      >  2:1047596/1‑140 (MQ=255)

                                                                                                                  ggTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCCCATGGGCGAggg                      >  2:245910/1‑140 (MQ=255)

                                                                                                                   gTCATCTCAATCACATTACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGgt                     >  1:4242751/1‑140 (MQ=255)

                                                                                                                        ctcAATTACATTACGCAC‑GCC‑C‑CGGGT‑CACTAGGTCGTTGATCTGCTTGCATATGAACGCTTACTTATCAAGGCGGCAGGCTACGTTGTTTTTAAAGCCGGAAAGATCAATATGTAGAGGCTCATGGGCGAGGGGGTAAc                >  2:1433869/1‑140 (MQ=255)

                                                                                                                                  ttACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATc      <  1:1178549/140‑1 (MQ=255)

                                                                                                                                  ttACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATc      <  1:2386103/140‑1 (MQ=255)

                                                                                                                                  ttACGCAC‑GCC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATc      >  2:2283763/1‑140 (MQ=255)

                                                                                                                                  ttACGCAC‑GAC‑C‑AGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGCTAAATACGCAGATGCTCATGGGCGAGGCGGTTACCCGTCGGagc      >  2:3587707/1‑138 (MQ=255)

                                                                                                                                    acgcac‑gcC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa    <  1:1960267/140‑1 (MQ=255)

                                                                                                                                    acgcac‑gcC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa    <  2:2581341/140‑1 (MQ=255)

                                                                                                                                    acgcac‑gcC‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTAGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa    <  2:4308695/140‑1 (MQ=255)

CACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCAC‑G‑C‑C‑CGGGT‑CATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAA  >  NZ_CP025268/3508998‑3509270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`