BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	400,598	+G	pseudogene (162/260 nt)	iraP →	anti‑adapter protein IraP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	400,594	1	.	G	97.0%	327.9 / 3.5	100	pseudogene (158/260 nt)	iraP	anti‑adapter protein IraP
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (58/39); total (60/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 116 total aligned reads displayed.

GTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑GGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400460‑400728

gttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑gg                                                                                                                                   <  1:1067867/140‑2 (MQ=255)

gttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑gg                                                                                                                                   <  1:3929226/140‑2 (MQ=255)

 ttATTTAAGCTTGCCCAAACAGAAGAAGAGTCGAACGAACTGTGTGCGCAGGTAGAAGCTTTGGCGATTATCGTCACTGCAATGCTTCGCAGTATGGCGCAAAATTGCCAACAGCGGTTGATTTGTCAGGTAG‑C‑ggtgtc                                                                                                                                   >  2:3694107/1‑136 (MQ=255)

 ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggc                                                                                                                                  <  1:3136091/140‑3 (MQ=255)

 ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggc                                                                                                                                  <  1:1590022/140‑3 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  1:3863451/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  2:3890322/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  2:3941486/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  2:3504470/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  2:41170/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  1:4078506/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  1:96225/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  2:1664538/140‑4 (MQ=255)

  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcg                                                                                                                                 <  1:1060597/140‑4 (MQ=255)

    tttAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑ggg‑ggcgct                                                                                                                               <  1:2704526/140‑6 (MQ=255)

        aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTAc                                                                                                                            <  1:3465713/140‑1 (MQ=255)

        aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTAc                                                                                                                            <  2:2314762/140‑1 (MQ=255)

         gCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACg                                                                                                                           <  1:3374677/140‑1 (MQ=255)

           ttGCCCAAAAAGAAGAAGAGCCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAg                                                                                                                         >  1:189469/1‑140 (MQ=255)

            tGCCCAAAAAGAAGAATAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTGTGGAGATTATCGTCACTGCAATGCGTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAgg                                                                                                                        >  2:651809/1‑140 (MQ=255)

              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTa                                                                                                                      >  2:4458219/1‑140 (MQ=255)

              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTa                                                                                                                      >  1:2287719/1‑140 (MQ=255)

              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTa                                                                                                                      >  2:3775976/1‑140 (MQ=255)

              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTa                                                                                                                      >  1:3622112/1‑140 (MQ=255)

              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAACAACAGAGGTTGATTGAACAGGTAG‑AGGGG‑GCGATGTACGAggaa                                                                                                                      >  2:3775988/1‑138 (MQ=255)

              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCATTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGCTGATCGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTa                                                                                                                      >  1:4045992/1‑140 (MQ=255)

                   aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGcc                                                                                                                 >  2:1198755/1‑140 (MQ=255)

                   aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGcc                                                                                                                 >  1:2109435/1‑140 (MQ=255)

                         gaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGc                                                                                                           >  1:2915368/1‑140 (MQ=255)

                               tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAAAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGTAt                                                                                                     >  1:4237301/1‑140 (MQ=255)

                                     gAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               <  1:1978741/140‑1 (MQ=255)

                                       aCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg                                                                                             <  2:2980130/140‑1 (MQ=255)

                                        cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:2520924/140‑1 (MQ=255)

                                        cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:3515809/140‑1 (MQ=255)

                                        atgtgtgCGCAGGTAGAAGCTTTGCAGATTATCGTAATTGCAATCCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTAATTCATCAGGTAG‑AGGGG‑GCGCTCTACGAGGTAAAGCCCGATGCCAGCATGCCTgacga                                                                                            <  2:3057536/139‑1 (MQ=255)

                                            gtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATGCCTGACGACGAt                                                                                        >  2:2890569/1‑140 (MQ=255)

                                               cgcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACg                                                                                     <  2:662007/140‑1 (MQ=255)

                                                  aGGTAGACGCTTTGGAGATTATCGTCACTGCCATGCTTCGCAATATGGCGCAAACTGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCGGTACGAGGTAAAGCCCGATTCCAGCATTCCTGACGACGATACGGAg                                                                                  >  1:2222640/1‑140 (MQ=255)

                                                   ggTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  1:842406/1‑140 (MQ=255)

                                                     tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  2:3865168/1‑140 (MQ=255)

                                                     tAGAAGCTTTGGAGATTACCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  2:4451027/1‑140 (MQ=255)

                                                       gAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGNCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctgct                                                                             >  1:241400/1‑140 (MQ=255)

                                                        aaGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGctgctg                                                                            <  1:4330390/140‑1 (MQ=255)

                                                            tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGCTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGGAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        >  1:987519/1‑140 (MQ=255)

                                                              tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:2444092/1‑140 (MQ=255)

                                                              tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:4417541/1‑140 (MQ=255)

                                                              tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:3196523/1‑140 (MQ=255)

                                                              tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:2444160/1‑140 (MQ=255)

                                                              tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGGACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:4517361/1‑140 (MQ=255)

                                                               ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  2:324938/140‑1 (MQ=255)

                                                               ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  1:630285/140‑1 (MQ=255)

                                                                     tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  1:4707103/111‑1 (MQ=255)

                                                                      aTCGTCGCTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                              >  2:783649/1‑140 (MQ=255)

                                                                      aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                              <  1:1159212/140‑1 (MQ=255)

                                                                      aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                              <  1:1159471/140‑1 (MQ=255)

                                                                      aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                              <  1:4922410/140‑1 (MQ=255)

                                                                       tCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                             <  1:1198755/140‑1 (MQ=255)

                                                                       tCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                             >  2:576982/1‑140 (MQ=255)

                                                                        cGTCACTGCAATGCATCGCAATATGGCGCAAAATGACCCACAGCGGTTGATTGATCAGGTAG‑A‑GGGCGCGCCGAACGAGGTAAAGCCAGATCCCAGCATTACTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                            <  2:1326580/140‑1 (MQ=255)

                                                                            aCTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGtt                                                        >  1:3750572/1‑140 (MQ=255)

                                                                             cTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTa                                                       <  1:1477022/140‑1 (MQ=255)

                                                                                  aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  2:4934164/140‑1 (MQ=255)

                                                                                   tGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAg                                                 >  1:2998851/1‑140 (MQ=255)

                                                                                        cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  2:144904/1‑140 (MQ=255)

                                                                                        cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  2:4067877/1‑140 (MQ=255)

                                                                                        cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACCGAGCTGCTGCGCGATTACGTAAAGCAGTTACTGAAGCATcc                                            >  2:3422409/1‑140 (MQ=255)

                                                                                          cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTc                                          >  2:2951950/1‑140 (MQ=255)

                                                                                            atatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                        <  2:4594166/140‑1 (MQ=255)

                                                                                               tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCCTTCCTGACGACGATAGGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  2:3316873/1‑140 (MQ=255)

                                                                                               tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  1:3732633/1‑140 (MQ=255)

                                                                                               tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  2:442943/1‑140 (MQ=255)

                                                                                               tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  1:2246905/1‑140 (MQ=255)

                                                                                               tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  2:3787381/1‑140 (MQ=255)

                                                                                                ggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt                                    >  2:4383667/1‑140 (MQ=255)

                                                                                                ggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt                                    >  2:3219149/1‑140 (MQ=255)

                                                                                                 gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                   <  1:1926665/140‑1 (MQ=255)

                                                                                                 gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                   <  2:2287719/140‑1 (MQ=255)

                                                                                                  cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                    <  1:3791487/122‑1 (MQ=255)

                                                                                                  cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                    >  2:3791487/1‑122 (MQ=255)

                                                                                                  cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  2:3834138/1‑140 (MQ=255)

                                                                                                  cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:4634754/1‑140 (MQ=255)

                                                                                                  cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:5001109/1‑140 (MQ=255)

                                                                                                    cAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaaaa                                >  1:279332/1‑140 (MQ=255)

                                                                                                                 aGCGGTTGATTGATCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTc                   >  1:363831/1‑140 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGCCGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:189469/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGCAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:117496/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:3853326/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:3925709/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:3300359/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:117748/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:4850811/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:1636928/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:136323/140‑1 (MQ=255)

                                                                                                                      ttgattgaTCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:5010845/140‑1 (MQ=255)

                                                                                                                             atCAGGTAGAA‑GGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAGGCTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       >  2:2174922/1‑140 (MQ=255)

                                                                                                                             atCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       <  1:5099127/140‑1 (MQ=255)

                                                                                                                             atCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       >  2:2070810/1‑140 (MQ=255)

                                                                                                                              tCAGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATa      >  2:1292261/1‑140 (MQ=255)

                                                                                                                                aGGTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa    >  2:3657313/1‑140 (MQ=255)

                                                                                                                                  gTAG‑AGGGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGt  >  2:4043904/1‑140 (MQ=255)

GTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAG‑A‑GGG‑GCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400460‑400728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`