BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	523,208	T→C	L300L (TTG→CTG)	rhsD →	rhs element protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	523,208	0	T	C	99.5%	1420.1 / NA	420	L300L (TTG→CTG)	rhsD	rhs element protein RhsD
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (198/220); minor base G (0/2); total (198/222)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

AGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATGACGCGCA  >  NZ_CP025268/523071‑523344

  cGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGAt                                                                                                                                      >  1:1379747/1‑140 (MQ=255)

    tGCGGAAGGGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGc                                                                                                                                    >  2:3061928/1‑140 (MQ=255)

    tGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGc                                                                                                                                    >  1:3542087/1‑140 (MQ=255)

       ggAAGATGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACg                                                                                                                                 <  2:2640004/140‑1 (MQ=255)

           gagGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGAccc                                                                                                                             <  2:2913501/140‑1 (MQ=255)

            agGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCg                                                                                                                            >  1:4025198/1‑140 (MQ=255)

             ggCCCGCCCCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  1:2738579/140‑1 (MQ=255)

             ggCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  1:2671279/140‑1 (MQ=255)

             ggCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  2:10285/140‑1 (MQ=255)

             ggCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  2:1934742/140‑1 (MQ=255)

             ggCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  2:3363043/140‑1 (MQ=255)

             ggCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCACTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  2:431492/140‑1 (MQ=255)

             gccccGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCgg                                                                                                                           <  1:4339132/138‑1 (MQ=255)

              gCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCATCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGAGCCGGc                                                                                                                          >  2:1499275/1‑140 (MQ=255)

              gCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGc                                                                                                                          <  1:1275264/140‑1 (MQ=255)

                   cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCCGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTGTCGGCGGTGGGGCTGATGCACGACCCGGCAGAcc                                                                                                                     >  2:2568723/1‑140 (MQ=255)

                   cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGCTGCACGACCCGGCATAcc                                                                                                                     >  2:1442095/1‑140 (MQ=255)

                   cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATAcc                                                                                                                     >  2:2819712/1‑140 (MQ=255)

                   cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATAcc                                                                                                                     >  1:4629215/1‑140 (MQ=255)

                     ccTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCg                                                                                                                   <  1:2203026/140‑1 (MQ=255)

                        ctTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGgag                                                                                                                >  1:2529966/1‑140 (MQ=255)

                        ctTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGgag                                                                                                                >  2:3767210/1‑140 (MQ=255)

                        ctTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGgag                                                                                                                <  1:504295/140‑1 (MQ=255)

                           cGCTATCGTCTGCTGACCGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACCCCCTCCCCGGTACCGCATACGGCCCCGCCAGCGGTATCCCCCTCTCGCCGGTGTGGCCGACGCACGACCCGGCATACCCggcgcgc                                                                                                             >  2:2759858/1‑135 (MQ=255)

                                tcttcttctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGcc                                                                                                        >  2:114251/1‑140 (MQ=255)

                                   tctcctGACAGTTCCCGCCCGCTCGCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGc                                                                                                     <  1:2354227/140‑1 (MQ=255)

                                        tgccAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTgcgc                                                                                                <  2:4106913/137‑1 (MQ=255)

                                          aCAGTTCCCGCCCTCGCTCAGCCTCAGCGTTCCCCGACACACTGCCCGTTACCGAATACGGCCCCGACAGGGGTAGCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGGCTGCCCGCGGCGCCa                                                                                              <  2:2100009/140‑1 (MQ=255)

                                              ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACCCTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTgg                                                                                          >  2:2268688/1‑140 (MQ=255)

                                              ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTgg                                                                                          >  1:5066115/1‑140 (MQ=255)

                                                cccgcccgCTCTCAGCCTCAGCGTTCCCCGGCACACTGCCCGATACCGGATACGGCCCCGACAGGGGTATCCGCCGTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCGGCGCCACTAGTg                                                                                        <  2:5043156/140‑1 (MQ=255)

                                                ccccccCTCTCTCAGCCTCAGCGTTCCCCGAGACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGTCTGATGCACTACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                        <  2:380409/140‑1 (MQ=255)

                                                cccGCCGTCCCCCAGCGCCAGCGTTCCCCGGCACGCCGCCCGGTGCCGAATACGGCCCCGCCAGGGGTACCCGCCTTTGGGCGGTGTGGCCGCTGCGCCACCCGGCATACCCGGAGGGCCGGCCCGCTGCGCCACTGGTg                                                                                        <  2:870819/140‑1 (MQ=255)

                                                cccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATCCGGCCCCAACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                        <  1:2878019/140‑1 (MQ=255)

                                                cccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                        <  2:4037302/140‑1 (MQ=255)

                                                cccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                        <  1:967557/140‑1 (MQ=255)

                                                cccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                        <  1:3980452/140‑1 (MQ=255)

                                                   gCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                                     <  1:447418/140‑1 (MQ=255)

                                                   gCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCgg                                                                                     <  2:1809237/140‑1 (MQ=255)

                                                   gCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGATGCGCCACTGGTGAgg                                                                                     <  1:1012383/140‑1 (MQ=255)

                                                     ccgctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  1:36949/137‑1 (MQ=255)

                                                     ccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  1:2451771/140‑1 (MQ=255)

                                                     ccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  2:4060473/140‑1 (MQ=255)

                                                     ccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  1:1170667/140‑1 (MQ=255)

                                                     ccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  1:3451827/140‑1 (MQ=255)

                                                     ccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  2:1695356/140‑1 (MQ=255)

                                                     ccTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  1:3877122/140‑1 (MQ=255)

                                                     ccTCTCTCAGCCTAAGCGTTCCCCGCCACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGCTGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                   <  1:2776756/140‑1 (MQ=255)

                                                      ctctctCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTac                                                                                  <  1:4019425/140‑1 (MQ=255)

                                                        ctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTacac                                                                                >  1:326597/1‑140 (MQ=255)

                                                        ctctcAGCCTCAGCGTTCCCCGACACACGGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGAGGCACGACCCGGCCTACCCGGACAGCCTGCCCGCTGCGCCACTGGTTCGGTacac                                                                                >  2:785112/1‑140 (MQ=255)

                                                               cctcagcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACg                                                                         >  1:1476349/1‑140 (MQ=255)

                                                               cctcagcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTGCGGCGGTGTGGCTGAGGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACg                                                                         <  1:1499275/140‑1 (MQ=255)

                                                                   agcGTTCCCCGACACACTGCCCGGTTCCGAACACGGCCCCGACAGGGGTATCCGCCGTTCGGCGGGGTGGCTGATGCACGACCCGGCATAGCCGGAGAGCCGGCCCGCTGCGCCACTGGTGCCGTGCACGTATACGGAAg                                                                     >  2:2235019/1‑140 (MQ=255)

                                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGGGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACTGAAg                                                                     >  2:1942503/1‑140 (MQ=255)

                                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAg                                                                     >  1:793281/1‑140 (MQ=255)

                                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAg                                                                     >  1:897256/1‑140 (MQ=255)

                                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAg                                                                     >  2:2174673/1‑140 (MQ=255)

                                                                   agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCAAGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACAGAAg                                                                     >  2:3946176/1‑140 (MQ=255)

                                                                         ccccGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTg                                                               >  2:3482413/1‑140 (MQ=255)

                                                                         ccccGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTg                                                               >  1:1197223/1‑140 (MQ=255)

                                                                            cGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAAc                                                            <  1:4820147/140‑1 (MQ=255)

                                                                               cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                        >  2:4840198/1‑109 (MQ=255)

                                                                               cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgc                                                         <  1:4142358/140‑1 (MQ=255)

                                                                               cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgc                                                         >  1:1753043/1‑140 (MQ=255)

                                                                                acacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgct                                                        <  1:1941871/140‑1 (MQ=255)

                                                                                  acTGCCCGGTACCGAATCCGGCCCCGACAGGCGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                      <  1:3204661/140‑1 (MQ=255)

                                                                                  acTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                      >  2:1330570/1‑140 (MQ=255)

                                                                                  acTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                      >  1:3470902/1‑140 (MQ=255)

                                                                                  acTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                      <  1:4170334/140‑1 (MQ=255)

                                                                                  acTGCCCGGAAACGAATACTGCGCCATCAGGGGTATGCGCCTTTGGGCGTTGTGGGGGTTGCACGACCTGGCTTACCCGGAGAGCCTGCCCGATGCGCTACTGGTGCGGTACACGTTTACGGATGCCGGTGAACTGCTgg                                                      <  2:163718/140‑1 (MQ=255)

                                                                                    tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCg                                                    >  2:580451/1‑140 (MQ=255)

                                                                                    tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCg                                                    >  1:4262490/1‑140 (MQ=255)

                                                                                      cccGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGt                                                  >  1:14430/1‑140 (MQ=255)

                                                                                          gTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTatat                                              <  1:1916144/140‑1 (MQ=255)

                                                                                              cGAATACGGCCCCGAGAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACGCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGtatagggcc                                          >  2:556704/1‑135 (MQ=255)

                                                                                              cGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGAcc                                          >  1:4406262/1‑140 (MQ=255)

                                                                                               gAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                                         <  1:2623976/140‑1 (MQ=255)

                                                                                                  tACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCgcag                                      <  1:2132147/140‑1 (MQ=255)

                                                                                                    cGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCgcagca                                    <  1:1638750/140‑1 (MQ=255)

                                                                                                    cGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCgcagca                                    <  2:1227482/140‑1 (MQ=255)

                                                                                                     ggCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCaa                                   >  1:4510232/1‑140 (MQ=255)

                                                                                                     ggCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCaa                                   >  1:718714/1‑140 (MQ=255)

                                                                                                     ggCCACGACAGGGGTATCCGCCTTTCGGCGGTGTGGCGGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATCCGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCaa                                   >  2:2039142/1‑140 (MQ=255)

                                                                                                       ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                                         >  1:1121482/1‑132 (MQ=255)

                                                                                                       ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATa                                 >  2:3655530/1‑140 (MQ=255)

                                                                                                       ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATa                                 <  2:3033114/140‑1 (MQ=255)

                                                                                                       ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATa                                 >  1:3345758/1‑140 (MQ=255)

                                                                                                       ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATa                                 >  1:4049182/1‑140 (MQ=255)

                                                                                                          cGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGc                              >  1:1980233/1‑140 (MQ=255)

                                                                                                                 ggTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                      >  1:2965381/1‑109 (MQ=255)

                                                                                                                     tCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCt                   >  1:2934107/1‑140 (MQ=255)

                                                                                                                     tCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCt                   >  1:3724395/1‑140 (MQ=255)

                                                                                                                       cGCCTTTCGGCGGTGTAGCTGATGCACGCCCCGGCATACCCGGAGAGCCTGCCCGCTGCTCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCttt                 <  1:2819869/140‑1 (MQ=255)

                                                                                                                          cTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCAc              >  1:4906702/1‑140 (MQ=255)

                                                                                                                          cTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGGGAACTGCTGGCGGGATATGACCGCAGCAATACGCAGTGGCGCGCTTTCAc              >  2:4430521/1‑140 (MQ=255)

                                                                                                                            ttCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGt            <  2:2447008/140‑1 (MQ=255)

                                                                                                                             tCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTa           >  1:4974590/1‑140 (MQ=255)

                                                                                                                               ggcggTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATg         <  1:4723472/140‑1 (MQ=255)

                                                                                                                                  ggTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATGAcg      <  1:3768206/140‑1 (MQ=255)

AGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATGACGCGCA  >  NZ_CP025268/523071‑523344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`