BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	541,580	G→A	A215T (GCG→ACG)	glxK →	glycerate 3‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	541,580	0	G	A	99.2%	918.3 / NA	256	A215T (GCG→ACG)	glxK	glycerate 3‑kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (142/112); minor base C (2/0); total (144/112)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

GCGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGT  >  NZ_CP025268/541444‑541713

gcgcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAacgc                                                                                                                                    >  2:801378/1‑140 (MQ=255)

 cgcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGcc                                                                                                                                   >  1:4795219/1‑140 (MQ=255)

  gcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCg                                                                                                                                  >  1:3536023/1‑140 (MQ=255)

    gTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGga                                                                                                                                <  2:3306763/140‑1 (MQ=255)

      ggCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTGGCATGTGACGTTGATAACCCGCTGGTAGCGGCACGCCGCGCGGCGACGGTGTTTGGCGCGCAAAAAGGGGCAACGCCGgaga                                                                                                                              <  2:3421798/140‑1 (MQ=255)

      ggCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGgaga                                                                                                                              <  2:4119567/140‑1 (MQ=255)

            cATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCg                                                                                                                        <  2:4819725/140‑1 (MQ=255)

             aTTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                       <  2:4028531/140‑1 (MQ=255)

             aTTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                       <  2:1726374/140‑1 (MQ=255)

             aTTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                       <  1:2977588/140‑1 (MQ=255)

               tGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaag                                                                                                                     >  1:4266041/1‑140 (MQ=255)

               tGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGAAAAAAGGGGCAACGCCGGAGATGGTCgaag                                                                                                                     <  1:1376897/140‑1 (MQ=255)

                gagaTGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaaga                                                                                                                    >  1:5050012/1‑140 (MQ=255)

                   atggatgAATGCGACCCGCGTCTGGCGATTTGCCCTATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACt                                                                                                                 <  2:1215720/140‑1 (MQ=255)

                      gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCACGGCGGGAGAGGGTGGAAGAACTTGa                                                                                                              >  1:4047567/1‑140 (MQ=255)

                      gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGa                                                                                                              >  1:2237357/1‑140 (MQ=255)

                      gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGa                                                                                                              >  1:4577660/1‑140 (MQ=255)

                      gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGa                                                                                                              >  2:352217/1‑140 (MQ=255)

                      gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGa                                                                                                              >  2:278382/1‑140 (MQ=255)

                        tgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAAc                                                                                                            <  1:3298506/140‑1 (MQ=255)

                            tGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGTTCGAAGAACTTGAACAggg                                                                                                        >  2:409496/1‑140 (MQ=255)

                            tGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAggg                                                                                                        >  1:1388809/1‑140 (MQ=255)

                            tGCGATCCGCGTCTGGCGAATTGACATATTGAAGTAGCATGTGACGTTGATGACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGAGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAggg                                                                                                        >  1:3056389/1‑140 (MQ=255)

                                aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTg                                                                                                    >  2:3131404/1‑140 (MQ=255)

                                aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTg                                                                                                    >  1:493042/1‑140 (MQ=255)

                                aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTg                                                                                                    >  1:2648704/1‑140 (MQ=255)

                                aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTg                                                                                                    >  1:1144830/1‑140 (MQ=255)

                                 tCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                                   <  1:37028/140‑1 (MQ=255)

                                  ccGCGTCTGGCGAATTGCCATATTGAAGGAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGCCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCa                                                                                                  <  2:1900576/140‑1 (MQ=255)

                                           gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGc                                                                                         >  2:1525029/1‑140 (MQ=255)

                                           gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGc                                                                                         >  2:3552598/1‑140 (MQ=255)

                                              aagtGCCATATTGAAGTAGCATGTGACGTTGATGACCCGCTGGGAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGCAGAACTTGATCAGGGGCTGCAAAATTACGCCCg                                                                                      <  2:4742802/137‑1 (MQ=255)

                                              aaTTGCCATATTGAAGTAGCATGTGACGTTGATACCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                                      <  2:4523645/140‑1 (MQ=255)

                                              aaTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                                      <  1:2067990/140‑1 (MQ=255)

                                                 tGCCATATTGAAGTAGCATGTGACGTTGATAACACGCTGGTAGGGGAACGCGGCGCGGCGGAGGTGTTTGGACCTCAAAAAGGGGCAACGCCGGAGATTGTCGAAGAACTTGAACAGTGGCTGCAAAATTACGCCCgtgt                                                                                   >  2:1055281/1‑140 (MQ=255)

                                                           aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAg                                                                         >  1:3846218/1‑140 (MQ=255)

                                                           aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAg                                                                         >  2:295695/1‑140 (MQ=255)

                                                                 cATGTGACGTTGATAACCCGCTGGTAGGGGCCCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACGTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCgaatt                                                                   >  2:1073374/1‑138 (MQ=255)

                                                                 cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACt                                                                   >  1:1988160/1‑140 (MQ=255)

                                                                 cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACt                                                                   >  1:390551/1‑140 (MQ=255)

                                                                 cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGGGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTATTTTACAACAGCAAAcg                                                                   >  2:1287181/1‑139 (MQ=255)

                                                                  aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                                  >  1:4327305/1‑140 (MQ=255)

                                                                  aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                                  >  2:4372303/1‑140 (MQ=255)

                                                                  aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                                  >  2:1897943/1‑140 (MQ=255)

                                                                   tgtgACGTTGGTAACCCGCTGGTTGGGGCACGAGGCTGGGGGGCGGGGTTTGGCCCGCAACAAGGGGCAACGCCGGAGAGGGTCGAAGAGCATGAACAGGGGCTGCAAAATTACGCCCGTGTGTTACAACAGCAAACTGa                                                                 >  2:4196474/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAAc                                                                                                                  >  2:2863929/1‑89 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                               >  2:3524363/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                               >  1:2108941/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                               >  2:594022/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGGTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                               >  2:2062534/1‑140 (MQ=255)

                                                                      gACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAt                                                              <  2:3702578/140‑1 (MQ=255)

                                                                       aCGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAtt                                                             >  1:1552377/1‑140 (MQ=255)

                                                                           tGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATg                                                         >  2:2110616/1‑140 (MQ=255)

                                                                            gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCTCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGACTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                                        <  2:2839529/140‑1 (MQ=255)

                                                                            gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                                        >  2:4186557/1‑140 (MQ=255)

                                                                                    gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAgg                                                >  1:2860761/1‑139 (MQ=255)

                                                                                         tAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcg                                           >  2:1832180/1‑140 (MQ=255)

                                                                                            gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                                        >  2:4528667/1‑140 (MQ=255)

                                                                                            gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                                        >  2:2237012/1‑140 (MQ=255)

                                                                                              gCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                                      <  1:1046504/140‑1 (MQ=255)

                                                                                                aCGCGGCGCGGCGGCGGTGTTTGGCGCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                                    >  2:4566799/1‑140 (MQ=255)

                                                                                                aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                                    <  1:3738124/140‑1 (MQ=255)

                                                                                                aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                                    >  1:2142651/1‑140 (MQ=255)

                                                                                                aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                                    <  1:836322/140‑1 (MQ=255)

                                                                                                aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                                    >  2:4969665/1‑140 (MQ=255)

                                                                                                  ggggcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGCACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                  <  2:4257242/138‑1 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                  >  1:3252523/1‑140 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                  >  2:3559095/1‑140 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                  >  1:2307083/1‑140 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                  >  2:4458704/1‑140 (MQ=255)

                                                                                                   cggcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGgcggcggcg                                 <  2:509295/140‑1 (MQ=255)

                                                                                                      cgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTg                              >  1:1773124/1‑140 (MQ=255)

                                                                                                        cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                            <  1:3409109/140‑1 (MQ=255)

                                                                                                        cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                            <  1:1257378/140‑1 (MQ=255)

                                                                                                         ggcggcggTGTTTGGCCTGCAAAAATGGGAAACGCCGGAGATAGTCGAAGAACTTGAACAAGAGCTGCAAAATTACGCCCGTGTTTCGCATCAGCAAACTGAAATAAATCTCTGCCCGATTGCGGGGGGCGGCGCTgcgg                           <  2:2562450/140‑1 (MQ=255)

                                                                                                         ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAGCTGAAATTAATGTGTGCCAGATGGCGGGCGGCGGCGCTgcgg                           <  2:3918668/140‑1 (MQ=255)

                                                                                                         ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                           <  2:2290657/140‑1 (MQ=255)

                                                                                                         ggcggcggGGTTGGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACCGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                           <  2:1372636/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                          <  1:4846109/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                          <  1:2940999/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                          <  1:664503/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                          <  2:2648704/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                          <  1:1022850/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAAGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                          <  1:4071939/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAACCTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                         <  1:2545907/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                         <  1:930812/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGAGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTTAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                         <  1:2834708/140‑1 (MQ=255)

                                                                                                              cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTTGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                      <  1:4680283/140‑1 (MQ=255)

                                                                                                              cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                      >  1:1479132/1‑140 (MQ=255)

                                                                                                              cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                      <  1:1497783/140‑1 (MQ=255)

                                                                                                                gtgtgtGGTCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                    <  2:1645139/140‑1 (MQ=255)

                                                                                                                gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                    <  1:2431901/140‑1 (MQ=255)

                                                                                                                gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                    <  2:2887118/140‑1 (MQ=255)

                                                                                                                     tGGCCCGCAAAAAGCGGCAACGCCGGAGATTGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGTCGGTATGGGTa               >  2:58431/1‑140 (MQ=255)

                                                                                                                        cccGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTg            >  2:462771/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg         >  1:3489255/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAGGGCGGGCGGCGGCGCTGCGGGCGGGATGGGTATTgcgg         >  2:1424863/1‑140 (MQ=255)

                                                                                                                            cAAAAAGGGGCAAAGGCGGAGATGGTTGAAAAAATTGAACAAGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggc        <  1:4411559/140‑1 (MQ=255)

                                                                                                                              aaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcgg      <  1:1623847/140‑1 (MQ=255)

                                                                                                                                  ggggCAACGCCGGAGATGGTCGAAGCACTGGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACCACAGCAAACTGAACTTCATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGt  <  2:5065273/140‑1 (MQ=255)

GCGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGT  >  NZ_CP025268/541444‑541713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`