BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	543,804	T→C	H180R (CAT→CGT)	allC ←	allantoate deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	543,804	0	T	C	99.6%	782.1 / NA	234	H180R (CAT→CGT)	allC	allantoate deiminase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (143/90); total (144/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 241 total aligned reads displayed.

GTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGA  >  NZ_CP025268/543668‑543939

gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCAGGCCAATTGATTGCCCATCACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                      >  1:4502812/1‑140 (MQ=255)

gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                      >  2:1791696/1‑140 (MQ=255)

 tttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCgg                                                                                                                                     <  1:4371639/140‑1 (MQ=255)

 tttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCgg                                                                                                                                     <  1:2357415/140‑1 (MQ=255)

   tGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAAGCGCATTCACCACGCCAATTGATTGCCCATTACTTTACAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGTCTTTAATATCCTGACGCGGAg                                                                                                                                   >  2:2271772/1‑140 (MQ=255)

         gCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCCCGCCAATTGATTGCCCATTACTTTCCAGCCCACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGagtgagtg                                                                                                                             <  2:4355092/140‑1 (MQ=255)

           cGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACATAGGCTTTAATATCCTGACGCGGAGTTAGTggg                                                                                                                           <  2:4606119/140‑1 (MQ=255)

           cGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTggg                                                                                                                           <  2:2679793/140‑1 (MQ=255)

           cGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTggg                                                                                                                           <  1:731900/140‑1 (MQ=255)

           cGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATGCCTTGGAGTAAGACACGGGGGTCCCCCTAACATTCAAAAAAACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTCACGCGGAGTTAGTggg                                                                                                                           >  2:1699258/1‑140 (MQ=255)

                   gTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                   <  2:474095/140‑1 (MQ=255)

                   gTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                   <  2:4064123/140‑1 (MQ=255)

                   gTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                   <  1:4785398/140‑1 (MQ=255)

                     tACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGaa                                                                                                                 >  2:857039/1‑140 (MQ=255)

                       ccGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAaga                                                                                                               >  1:847286/1‑140 (MQ=255)

                          tataTCGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTa                                                                                                            >  2:4714732/1‑140 (MQ=255)

                            taACGACGCTGCCCGACAATGGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTaaa                                                                                                          >  2:5511/1‑140 (MQ=255)

                               cgacgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATcc                                                                                                       >  1:4900357/1‑140 (MQ=255)

                                gaggCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                      <  2:4124427/137‑1 (MQ=255)

                                gacgCTGCCCGACAATCGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                      <  1:2830847/140‑1 (MQ=255)

                                  cgCTGCCCGACAATTGCATCCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTGAGTGGGGCGTTCGGAAGCGTAAATCCGCa                                                                                                    >  2:3571310/1‑140 (MQ=255)

                                    cTGCCCGACAATTGCATTCACTACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTAGGAAGAGTAAATCCGCAAg                                                                                                  >  2:3431170/1‑140 (MQ=255)

                                              aTTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgc                                                                                        <  1:749619/140‑1 (MQ=255)

                                               ttGCATTCACCACGCCAATTAATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgca                                                                                       >  2:3788920/1‑140 (MQ=255)

                                                 gCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcatc                                                                                     >  1:3272177/1‑140 (MQ=255)

                                                 gCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcatc                                                                                     >  1:3017232/1‑140 (MQ=255)

                                                   aTTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGa                                                                                   >  1:1005881/1‑140 (MQ=255)

                                                       accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaa                                                                               >  1:4463510/1‑140 (MQ=255)

                                                       accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATAGCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGGAAATCCGCAAGCCTGCATCGCATCGACaaa                                                                               >  2:1542126/1‑140 (MQ=255)

                                                        ccacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaaa                                                                              <  1:246084/140‑1 (MQ=255)

                                                         cacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAAc                                                                             >  2:4449469/1‑140 (MQ=255)

                                                            gctaaTTGATTGCCCATTACTTTCCAGCACGCAGCCCTGTTCGATATGCAGTTCAACAGAGGCTGTAATATCCTGACGCGGCGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCGCGCCTTCATCGCATCGACAAAACTAt                                                                          <  2:2378214/137‑1 (MQ=255)

                                                            gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                          >  1:2163767/1‑140 (MQ=255)

                                                            gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                          >  1:4277783/1‑140 (MQ=255)

                                                             ccAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAtt                                                                         <  2:3266265/140‑1 (MQ=255)

                                                                  tgattgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCtt                                                                    <  2:115395/140‑1 (MQ=255)

                                                                  tgattgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCtt                                                                    <  1:1324277/140‑1 (MQ=255)

                                                                     ttgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGGTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                                 >  2:1361126/1‑140 (MQ=255)

                                                                       gCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCa                                                               >  1:543779/1‑140 (MQ=255)

                                                                        cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcat                                                                                      <  1:958756/116‑1 (MQ=255)

                                                                        cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                              <  2:1410114/140‑1 (MQ=255)

                                                                        cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                              <  1:4049343/140‑1 (MQ=255)

                                                                        cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                              <  1:3369488/140‑1 (MQ=255)

                                                                        cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGGTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                              <  2:4515359/140‑1 (MQ=255)

                                                                             tACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAg                                                         <  1:4871849/140‑1 (MQ=255)

                                                                             tACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAg                                                         <  1:3863097/140‑1 (MQ=255)

                                                                               cTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGat                                                       >  2:4160530/1‑140 (MQ=255)

                                                                                  tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCGGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAGGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATAtt                                                    >  2:4264619/1‑140 (MQ=255)

                                                                                     aacacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCg                                                 <  1:928994/138‑1 (MQ=255)

                                                                                       cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                               <  2:4303304/140‑1 (MQ=255)

                                                                                        acacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCGGACGCGGAGTTAGTGGGGCGTTTGGAAGAGTACGTCCGCAAGCCCTCATCGCACCGTCCAAACTCTTTACTTTGTCATCACAGTTAGACCGCAc                                              <  2:2128736/140‑1 (MQ=255)

                                                                                         cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGTAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcg                                             >  1:2189815/1‑140 (MQ=255)

                                                                                            aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                          >  1:3489923/1‑140 (MQ=255)

                                                                                            aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                          >  2:5056833/1‑140 (MQ=255)

                                                                                            aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                          >  2:4960671/1‑140 (MQ=255)

                                                                                            aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                          >  1:4595725/1‑140 (MQ=255)

                                                                                             gCCCTGTTCAATCTGCAGTTCAACAAAGGCTGTAATAGTCTGACGCGAAGTTAGTGGGGCGTTCGGAAGAGTACACCCGCAAGCCTTCATCGCATCGCCAAAACTATTTCCCTTGGCATCCCAGCTACTCCGCAcgtcgt                                         <  2:2643145/140‑1 (MQ=255)

                                                                                             ccccTGTTCAATATGCAGTTCAACAAATGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTGCCTTTGGCATCACAGATATTCCGCAcgtcgt                                         <  1:4785118/139‑1 (MQ=255)

                                                                                                   ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGAt                                   >  2:65881/1‑140 (MQ=255)

                                                                                                   ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGAt                                   >  1:1678567/1‑140 (MQ=255)

                                                                                                   ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGCTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGGATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGAt                                   >  2:2181490/1‑140 (MQ=255)

                                                                                                    tCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAGTCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTGTGGCATCACAGATATTCCGCACGTCGTCAGGAtt                                  >  2:4535491/1‑140 (MQ=255)

                                                                                                       atatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                               <  1:1289527/140‑1 (MQ=255)

                                                                                                        tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATGGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCCGGATTCGcc                              >  2:4857384/1‑140 (MQ=255)

                                                                                                        tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                              >  1:1949982/1‑140 (MQ=255)

                                                                                                        tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                              >  2:3939044/1‑140 (MQ=255)

                                                                                                        tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                              >  1:3279314/1‑140 (MQ=255)

                                                                                                        tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                              >  1:3304358/1‑140 (MQ=255)

                                                                                                        tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACGATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                              >  2:2984403/1‑140 (MQ=255)

                                                                                                             aggtCAACAAAGGCTTTAATATCCTGACGCGGAGGGAGTGGGGCGTTCGGGAGAGTAAATCCGCAAGCCTTGATCGCATCGACAAAACTAGTTCCTTTGGGATCACAGATAGTCCGCACGTCGCCAGGGGTCGCCAGacc                         >  2:1717697/4‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcgc                         >  2:144455/1‑138 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  2:1907201/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  1:2028810/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  1:2960519/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  1:3965354/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  1:3160028/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  2:3663727/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  1:626721/1‑140 (MQ=255)

                                                                                                             aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGATAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                         >  1:3821462/1‑140 (MQ=255)

                                                                                                               ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                       >  1:3725497/1‑140 (MQ=255)

                                                                                                               ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                       >  1:4167008/1‑140 (MQ=255)

                                                                                                                  aacaaAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGCTATTCCGCACGTCGTCAGGATTCGCCAGCCCaaaaa                    >  2:2795176/1‑140 (MQ=255)

                                                                                                                         gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCACAAATAttttt             >  2:3114559/1‑140 (MQ=255)

                                                                                                                         gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt             >  1:2773443/1‑140 (MQ=255)

                                                                                                                         gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt             >  1:2655974/1‑140 (MQ=255)

                                                                                                                         gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTGGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt             >  1:4559566/1‑140 (MQ=255)

                                                                                                                          cTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTa            <  1:537152/140‑1 (MQ=255)

                                                                                                                          cTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTa            <  1:261452/140‑1 (MQ=255)

                                                                                                                           tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc           <  2:208650/140‑1 (MQ=255)

                                                                                                                           tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc           <  2:4277783/140‑1 (MQ=255)

                                                                                                                           tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc           <  1:1732497/140‑1 (MQ=255)

                                                                                                                           tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc           <  2:3035138/140‑1 (MQ=255)

                                                                                                                             tAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg         <  2:3726306/140‑1 (MQ=255)

                                                                                                                             tAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAACAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg         <  1:5033154/140‑1 (MQ=255)

                                                                                                                              aaTATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGc        <  1:4632185/140‑1 (MQ=255)

                                                                                                                                   ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag   >  1:2431555/1‑140 (MQ=255)

                                                                                                                                   ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag   >  1:1932855/1‑140 (MQ=255)

                                                                                                                                   ccTGACGCGGAGTTAGTGGGGCGTTAGGAAGCGTAAATCCGAAAGACTTCATCGCATCGACAAAACTATTTCCTTTGGAAACACAGATATTCCGCACGTCGTCAGGATTCTCAAGCCCAAAGATGTTTTTACTGGCCCag   >  2:3149394/1‑140 (MQ=255)

                                                                                                                                    cTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                              <  1:5062341/80‑1 (MQ=255)

                                                                                                                                    cTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCaga  <  2:4463510/140‑1 (MQ=255)

GTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGA  >  NZ_CP025268/543668‑543939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`