BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	671,996	A→C	F613V (TTT→GTT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	671,996	0	A	C	100.0%	1093.2 / NA	326	F613V (TTT→GTT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (141/185); total (141/185)

Only 100 of 327 total aligned reads displayed.

ATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCA  >  NZ_CP025268/671861‑672130

aTTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGt                                                                                                                                    >  1:3263031/1‑140 (MQ=255)

aTTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGt                                                                                                                                    >  2:665208/1‑140 (MQ=255)

         tCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCt                                                                                                                           >  1:1953690/1‑140 (MQ=255)

           atatCAGCCGGAGAAGCAAACATCATAAACAGCCGAACGGTGTCCGCGCCGTAACGTTCAACCATCCCCCGCGGGTCGAGACCGTTGTTCTTCGCCTTGCACATTTTGCTCATGCCTGTACAACCCACGTCAGGGCCTGc                                                                                                                         >  2:232334/1‑140 (MQ=255)

           atatCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGc                                                                                                                         >  1:3795161/1‑140 (MQ=255)

           atatCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTCGCTCATGCCGGTATAAACCAGTTCATGGCCttc                                                                                                                         >  2:1935230/1‑138 (MQ=255)

              tCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGc                                                                                                                      >  1:2795332/1‑140 (MQ=255)

                  ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCt                                                                                                                  >  2:4324847/1‑140 (MQ=255)

                   cGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCtt                                                                                                                 >  1:4976792/1‑140 (MQ=255)

                   cGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCtt                                                                                                                 <  2:2458403/140‑1 (MQ=255)

                     gagaAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTc                                                                                                               >  1:4058610/1‑140 (MQ=255)

                     gagaAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTc                                                                                                               >  2:3288107/1‑140 (MQ=255)

                      agaAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCAGGGCCTGCCGCATCTTTCg                                                                                                              >  1:1599079/1‑140 (MQ=255)

                          tcAAACACCAGATACAGCCACACGGTGTCCGCGCCGTAACGTTCAACCTTCAACTGCGGCTCGATACCGTTGTTCTTCGCCTTCGACCTTTTGCTCATGCCGGTATCCACCAGTTCAGGGCCTGCCGCATCTTTCGCttt                                                                                                          <  2:1056060/139‑1 (MQ=255)

                           cAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTc                                                                                                         <  1:2564807/140‑1 (MQ=255)

                           cAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTc                                                                                                         <  1:1484992/140‑1 (MQ=255)

                            aaaCATCATAAACAGACGAATGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGCCATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCa                                                                                                        <  2:821903/140‑1 (MQ=255)

                            aaaCATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCa                                                                                                        <  2:556455/140‑1 (MQ=255)

                            aaaCATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCa                                                                                                        <  1:269397/140‑1 (MQ=255)

                                 tcatAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATa                                                                                                   <  1:3040606/140‑1 (MQ=255)

                                  catAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc                                                                                                  >  1:2362088/1‑140 (MQ=255)

                                  catAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc                                                                                                  >  1:4303929/1‑140 (MQ=255)

                                  catAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc                                                                                                  >  2:4635745/1‑140 (MQ=255)

                                    tAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATGACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACgg                                                                                                >  2:2640725/1‑140 (MQ=255)

                                    tAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACgg                                                                                                >  1:2840469/1‑140 (MQ=255)

                                       aCAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCt                                                                                             <  2:4723635/140‑1 (MQ=255)

                                           aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtct                                                                                         >  2:3383229/1‑140 (MQ=255)

                                           aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtct                                                                                         >  2:1258122/1‑140 (MQ=255)

                                           aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtct                                                                                         >  2:2245343/1‑140 (MQ=255)

                                             gAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCg                                                                                       <  1:4842145/140‑1 (MQ=255)

                                              aaCGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt                                                                                      <  2:4357918/140‑1 (MQ=255)

                                                cGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATACACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                                    <  1:2426861/140‑1 (MQ=255)

                                                cGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                                    <  2:3488/140‑1 (MQ=255)

                                                  gtgtCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACg                                                                                  <  2:2523435/140‑1 (MQ=255)

                                                     tCCGCGCCGTAACGTTCAACCCTCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGCTACGGCCTTTCTCGTCACGTTc                                                                               <  2:3518151/140‑1 (MQ=255)

                                                     tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTc                                                                               <  1:9508/140‑1 (MQ=255)

                                                     tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTc                                                                               <  1:856854/140‑1 (MQ=255)

                                                     tCCCCGCCGTATCCGTCAATCATCACCGGTGGGGCGACGCCGTTGGTCTTCGACGTGTACATTTTGCTCGTGCCGGTATAAGCCAGTTCATGTCCGGCCGCATCTTTCGCTTTCACTAGACGGCCTTTCTCGTCACTTTc                                                                               <  2:315423/140‑1 (MQ=255)

                                                       cgcgCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTGCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCaa                                                                             >  1:4553984/1‑140 (MQ=255)

                                                         cgcCCTAACGTTCAACCATCACCTGCGCGTCGATACCGGTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAACCCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg                                                                           <  1:1222643/140‑1 (MQ=255)

                                                          gcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGa                                                                          <  1:1001232/140‑1 (MQ=255)

                                                             gTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTTATGGCCTGCCGCATCTTTCGCTTTCACGCTACGGCCTTTCTCGTCACGTTCAACGATaa                                                                       >  2:4874483/1‑139 (MQ=255)

                                                               aaCGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCa                                                                     >  2:2516116/1‑140 (MQ=255)

                                                                   ttCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCaa                                                                 <  2:3254527/140‑1 (MQ=255)

                                                                     cAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAcc                                                               >  1:2987449/1‑140 (MQ=255)

                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCg                                                              >  1:4109171/1‑140 (MQ=255)

                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCg                                                              >  2:2950673/1‑140 (MQ=255)

                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCg                                                              >  2:3688835/1‑140 (MQ=255)

                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCg                                                              >  1:358404/1‑140 (MQ=255)

                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGAATTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCGGCCGCGTCTTTCGCTTTCACGATACCGCCTTTCTCGTCACCTTCAACGATATCATCAACCg                                                              >  2:2799100/1‑140 (MQ=255)

                                                                       aCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgg                                                             <  2:5054410/140‑1 (MQ=255)

                                                                        ccATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCggg                                                            >  1:2177381/1‑140 (MQ=255)

                                                                         cATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCCCGTTCAACGATAGCATCAACCgggg                                                           <  1:1853739/140‑1 (MQ=255)

                                                                         cATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgggg                                                           <  1:335700/140‑1 (MQ=255)

                                                                           tCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaa                                                         <  1:474655/140‑1 (MQ=255)

                                                                             aCCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAAc                                                       >  2:4369442/1‑140 (MQ=255)

                                                                                  cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTCTAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGGAACCCAGt                                                  >  2:393834/1‑140 (MQ=255)

                                                                                  cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGt                                                  >  1:1048250/1‑140 (MQ=255)

                                                                                     gTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTAc                                               >  1:711544/1‑140 (MQ=255)

                                                                                         aTACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTc                                           >  2:3757124/1‑140 (MQ=255)

                                                                                             cGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCg                                       <  1:5056447/140‑1 (MQ=255)

                                                                                             cGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTGCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGGTTCGTTCGCCg                                       >  2:3595972/1‑140 (MQ=255)

                                                                                               ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGACACCCCATTACGTTCGCCGtt                                     >  2:3881519/1‑140 (MQ=255)

                                                                                               ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGtt                                     >  2:1601021/1‑140 (MQ=255)

                                                                                               ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGCTACGTTCGCCGtt                                     >  2:3434449/1‑140 (MQ=255)

                                                                                               ttgttCTTCGACTTGGAAATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCACGTCACGTTCCACGATAGCATGAACCGGGGAACCCCCGTTACGTTCGCCGtt                                     >  2:1977080/1‑140 (MQ=255)

                                                                                               ttgttCTTCGACTTCGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTAGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGtt                                     <  1:3075164/140‑1 (MQ=255)

                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                <  2:2840469/140‑1 (MQ=255)

                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                <  1:4170329/140‑1 (MQ=255)

                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                >  2:1738320/1‑140 (MQ=255)

                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGGTACGTTCGCCGTTTTCGc                                >  1:1103477/1‑140 (MQ=255)

                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCAGGGCCTGCCGCATCTTTCTCTTTCACGGTACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                <  2:207826/140‑1 (MQ=255)

                                                                                                    cttcGACTTGGACATTTTGCTCAGGCCGCTATAAACCAGGTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTCTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                <  2:4273089/140‑1 (MQ=255)

                                                                                                    catagACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATCGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCTCGACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                <  2:3049700/136‑1 (MQ=255)

                                                                                                       cGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa                             >  1:811670/1‑140 (MQ=255)

                                                                                                       cGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa                             >  1:4706943/1‑140 (MQ=255)

                                                                                                         ccTTGGACATTTTGCTCATGCCGGTATAACCCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATCCGGCCTTTCTCGTCACGTTCAACGATAGCATCATCCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACa                           <  2:876650/139‑1 (MQ=255)

                                                                                                         aCTTGGACATTTTTCTCATGCCGGTATAAACCAGTTCATGGCCTGCCACATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCACGTGACGTGCACCGTTTTGGCCAACa                           >  2:4009914/1‑140 (MQ=255)

                                                                                                         aCTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACa                           >  2:2980276/1‑140 (MQ=255)

                                                                                                         aCTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACa                           <  1:1536274/140‑1 (MQ=255)

                                                                                                            tGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACAtag                        >  2:1047969/1‑140 (MQ=255)

                                                                                                            tGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACAtag                        <  1:1973647/140‑1 (MQ=255)

                                                                                                            tGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACAtag                        >  2:4473004/1‑140 (MQ=255)

                                                                                                                cATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGa                    <  1:3114957/140‑1 (MQ=255)

                                                                                                                   tttGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCCACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGGCAACAGAGGAGAAgg                 >  2:1105285/1‑140 (MQ=255)

                                                                                                                    ttGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                <  1:3492773/124‑1 (MQ=255)

                                                                                                                     tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  2:1798583/140‑1 (MQ=255)

                                                                                                                     tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  1:1700261/140‑1 (MQ=255)

                                                                                                                     tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  1:2290262/140‑1 (MQ=255)

                                                                                                                     tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  1:3844789/140‑1 (MQ=255)

                                                                                                                     tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  1:3998037/140‑1 (MQ=255)

                                                                                                                     tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTGCACGAGACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  1:521428/140‑1 (MQ=255)

                                                                                                                      gCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTTACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCAt              <  2:4751584/140‑1 (MQ=255)

                                                                                                                      gCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCAt              <  1:4635745/140‑1 (MQ=255)

                                                                                                                          aTGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc          <  2:1497463/140‑1 (MQ=255)

                                                                                                                            gCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa        >  1:1781715/1‑140 (MQ=255)

                                                                                                                            gCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa        <  1:1392033/140‑1 (MQ=255)

                                                                                                                                gTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAc    <  1:3935888/140‑1 (MQ=255)

                                                                                                                                gTATAAACCAGGGCATGGCCTGCCGCAGCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAc    <  1:1644064/140‑1 (MQ=255)

                                                                                                                                  agaaacCAGTTCATGGCCTGCCGCATCTTTCGCTTTCCCGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCa  <  2:4960466/138‑1 (MQ=255)

ATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCA  >  NZ_CP025268/671861‑672130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`