BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	774,169	A→G	A123A (GCA→GCG)	ybgC →	tol‑pal system‑associated acyl‑CoA thioesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	774,169	0	A	G	100.0%	822.2 / NA	247	A123A (GCA→GCG)	ybgC	tol‑pal system‑associated acyl‑CoA thioesterase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (144/103); total (144/103)

Only 100 of 247 total aligned reads displayed.

TACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTAT  >  NZ_CP025268/774033‑774305

tACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCtt                                                                                                                                       <  2:4916282/140‑1 (MQ=255)

tACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCtt                                                                                                                                       <  1:4879864/140‑1 (MQ=255)

   aGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTccc                                                                                                                                    <  2:676667/140‑1 (MQ=255)

    gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCa                                                                                                                                   >  2:2100808/1‑140 (MQ=255)

    gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCa                                                                                                                                   >  2:1850125/1‑140 (MQ=255)

    gACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGGTGTTTGCGTTGACCCACCCAAAATGAAGCGTCGTGCGCTTCCCa                                                                                                                                   >  1:1130873/1‑140 (MQ=255)

     aCTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCaa                                                                                                                                  >  1:595542/1‑140 (MQ=255)

      cTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAg                                                                                                                                 >  1:1240850/1‑140 (MQ=255)

        gAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTGTGGTTGTTTGCGTTGACCCACTCAAAGGGAAGCCGCGTGCGCTTCCCAAGTc                                                                                                                               >  2:1609933/1‑140 (MQ=255)

        gAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCAGTCAAAATGAAGCCTCGTGCGCTGCCGAAGtt                                                                                                                               >  2:4480498/1‑139 (MQ=255)

        gAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTc                                                                                                                               >  1:3752567/1‑140 (MQ=255)

        gAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTc                                                                                                                               >  1:969833/1‑140 (MQ=255)

              aCATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGt                                                                                                                         <  1:3577902/140‑1 (MQ=255)

                aTCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTcg                                                                                                                       <  1:702875/140‑1 (MQ=255)

                 tCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTcgc                                                                                                                      >  2:3159357/1‑140 (MQ=255)

                 tCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTcgc                                                                                                                      <  1:2177400/140‑1 (MQ=255)

                 tCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACGCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTcgc                                                                                                                      <  1:2154122/140‑1 (MQ=255)

                   aaTGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCgg                                                                                                                    <  1:4172347/140‑1 (MQ=255)

                          ggCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTaa                                                                                                             >  1:4383927/1‑140 (MQ=255)

                          ggCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTaa                                                                                                             >  1:252789/1‑140 (MQ=255)

                          ggCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTaa                                                                                                             >  1:1020277/1‑140 (MQ=255)

                          ggCACAGCTTTGTCTTTCACGCAACCTCTCGGCAACGCCGAGCATACTTCGACGAATGAAGCAGAGGTTCTGCTTGATTGCGTGGCCCCACTCAAAATGAAAACTCTGACGCGACCCAAGGCGATGGTCGCGAAGtttga                                                                                                             >  2:165332/1‑138 (MQ=255)

                             aCCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCa                                                                                                          <  1:633181/140‑1 (MQ=255)

                               ctctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGGGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGt                                                                                                        >  1:517662/1‑140 (MQ=255)

                                 ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                      <  1:605628/140‑1 (MQ=255)

                                 ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                      <  1:3228255/140‑1 (MQ=255)

                                 ctTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                      <  1:3512356/140‑1 (MQ=255)

                                 ctTTGGTGTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtga                                                                                                      <  2:3937588/140‑1 (MQ=255)

                                  tttGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgac                                                                                                     <  1:3512144/140‑1 (MQ=255)

                                   ttGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGGGGTTAAGTAGtgacg                                                                                                    >  2:139121/1‑139 (MQ=255)

                                   ttGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgact                                                                                                    <  2:1137768/140‑1 (MQ=255)

                                     ggTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGtgactga                                                                                                  <  1:1865896/140‑1 (MQ=255)

                                         ttCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGGTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATg                                                                                              >  1:5013146/1‑140 (MQ=255)

                                          tCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGa                                                                                             <  1:2185564/140‑1 (MQ=255)

                                           cACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                            >  1:1042505/1‑140 (MQ=255)

                                           cACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                            <  1:1088745/140‑1 (MQ=255)

                                           cACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTAGTGTCGCGGAGTTTAAGCCATGACTGACATTaa                                                                                            >  1:1601487/1‑140 (MQ=255)

                                           cACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTCCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCATTGACTGACATGaa                                                                                            >  1:4625375/1‑140 (MQ=255)

                                           cACGCAACGTATTGTCAACGCCGAGAATACTTAGCTGAATGAAGCAGCGGTTCTGGTTATTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCGGTTGTCGCGGAGTTTAAGCAGTGACTGACATGaa                                                                                            >  2:3102553/1‑140 (MQ=255)

                                                 aCGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCt                                                                                      <  1:1362003/140‑1 (MQ=255)

                                                     aTTGTCAACGCCGAGACTACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGATCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                                  <  1:2837735/140‑1 (MQ=255)

                                                     aTTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                                  <  2:5056007/140‑1 (MQ=255)

                                                     aTTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGAt                                                                                  <  1:3293280/140‑1 (MQ=255)

                                                        gTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTg                                                                               <  1:2080145/140‑1 (MQ=255)

                                                            aCGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTcc                                                                           >  2:3490796/1‑140 (MQ=255)

                                                             cGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCt                                                                          <  2:1513191/140‑1 (MQ=255)

                                                              gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTg                                                                         >  2:5015983/1‑140 (MQ=255)

                                                              gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTg                                                                         <  1:1952704/140‑1 (MQ=255)

                                                              gCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTg                                                                         >  2:568494/1‑140 (MQ=255)

                                                                 gagaATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAg                                                                      >  1:2433852/1‑140 (MQ=255)

                                                                   gaATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGc                                                                    >  2:4072339/1‑140 (MQ=255)

                                                                   gaATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGGTTAAGCAGGGACTGACATGAATATCCTTGATTTGTTCCTGAAGGc                                                                    >  2:1682265/1‑140 (MQ=255)

                                                                         tttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGGTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                              >  2:2674323/1‑140 (MQ=255)

                                                                         tttGCCGAATGAAGCACAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCt                                                              <  2:2676681/140‑1 (MQ=255)

                                                                          ttGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTTAAGATGAAGCCTCGTGCGCTTCCCAAGTCGTTTGTCCCGGAGTTTAAGCAGTGACTGAGATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCtt                                                             <  2:3649524/140‑1 (MQ=255)

                                                                               gaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGt                                                        <  1:3114210/140‑1 (MQ=255)

                                                                               gaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGt                                                        <  1:4751926/140‑1 (MQ=255)

                                                                               gaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACACAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCATGATTTGTTCCTGAAGGCTAGCCTTCTGGt                                                        <  2:4674713/140‑1 (MQ=255)

                                                                                  tgaaGCCGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTac                                                     <  2:4941852/140‑2 (MQ=255)

                                                                                     aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACt                                                  >  1:1793046/1‑140 (MQ=255)

                                                                                     aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACt                                                  >  1:1677404/1‑140 (MQ=255)

                                                                                     aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACt                                                  >  2:1336905/1‑140 (MQ=255)

                                                                                     aGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGGGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACt                                                  >  2:1885721/1‑140 (MQ=255)

                                                                                          agGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCa                                             >  1:1396045/1‑140 (MQ=255)

                                                                                          agGGTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGGCTGACATGAAGATCCTTGATTTGTTCCTGAAGCCTGGGCTTCGGGTTAAACTTATCa                                             >  2:3384205/1‑140 (MQ=255)

                                                                                            gTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATg                                           <  1:748543/140‑1 (MQ=255)

                                                                                              tCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTAATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGtt                                         >  2:2923565/1‑140 (MQ=255)

                                                                                               cTGGTTGTTTGCGTTGACCCACTCAAAATGAAGACTAGTGCGCTTCCGAAGTATATTGTCGCGGAGATTAAGAAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTCCTGGTTAAACTTATCATGttg                                        >  2:430963/1‑140 (MQ=255)

                                                                                                      tttGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttga                                 >  2:2227292/1‑140 (MQ=255)

                                                                                                       ttGCGTTGACCGACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGGTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACGTATCATGttgattttgat                                >  2:3633669/1‑140 (MQ=255)

                                                                                                       ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTTACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttggt                                >  2:107209/1‑138 (MQ=255)

                                                                                                       ttGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGttgattttgat                                >  2:4646891/1‑140 (MQ=255)

                                                                                                               aCCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGGTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGttttt                        >  2:554282/1‑140 (MQ=255)

                                                                                                                    cTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATc                   >  2:2157794/1‑140 (MQ=255)

                                                                                                                    cTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCGTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATc                   >  1:2695176/1‑140 (MQ=255)

                                                                                                                     tCAAAGTGACGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGTCTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCg                  <  2:379847/140‑1 (MQ=255)

                                                                                                                     tCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCg                  <  1:2994878/140‑1 (MQ=255)

                                                                                                                     tCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGGTAAACTTATCATGTTGATTTTGATTGGTTTTTCAAGCg                  <  2:384907/140‑1 (MQ=255)

                                                                                                                         aaTGAAGCCTCGTGCGCTTCCCAGGTCTATGGTCGCGGAGTTTAAGCAGTGACTGACATGAATATGCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCAGGTTGATTTTGTTTGGTTTTTCAGTCGCagt              >  2:4028513/1‑138 (MQ=255)

                                                                                                                         aaTGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATc              <  1:1540741/140‑1 (MQ=255)

                                                                                                                          aTGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCt             <  2:3470921/140‑1 (MQ=255)

                                                                                                                          aTGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCCTCTGGTTAAACTTATCATGTTGATTTTGCTTGGTTTTTCAATCGCATCt             <  1:95488/140‑1 (MQ=255)

                                                                                                                          aTGAAGCCGCGTGCGCTTACCAAGTCTATTGTAGCGGAGTTTAAGCAGTGACTGAAATGAATATACTTGATTTGTACCTGAAGGCTAGCCTTCTGGTTAAACTTATCAGGTGGATTTTGATTGGTTCTTCAATCGCATCt             <  2:1805632/140‑1 (MQ=255)

                                                                                                                              aGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGCCTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTggg         >  1:2270415/1‑140 (MQ=255)

                                                                                                                              aGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTggg         >  1:3561533/1‑140 (MQ=255)

                                                                                                                              aGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTggg         >  1:3022489/1‑140 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        >  1:204302/1‑140 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        >  2:1500941/1‑140 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  1:2272196/140‑1 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  1:2946996/140‑1 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  1:4960651/140‑1 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  1:4913603/140‑1 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  1:44986/140‑1 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  1:4072339/140‑1 (MQ=255)

                                                                                                                               gCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGc        <  2:907836/140‑1 (MQ=255)

                                                                                                                                   cGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCatt    >  1:4566265/1‑140 (MQ=255)

                                                                                                                                   cGTGCGCTTCCCAAGTCTATTGTCGCGGAGGTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCatt    >  2:1469581/1‑140 (MQ=255)

                                                                                                                                    gTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCatta   >  1:758662/1‑140 (MQ=255)

                                                                                                                                     tGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCattat  >  1:85594/1‑140 (MQ=255)

                                                                                                                                     tGCGCTTCCAACGTCTATTGTCTCGGAGCTTAAGCAGTGCCTGACATGAATAGACTTGATTTGTTCCTGAAGGCTAGCCTGTTGGTCAAACTCATCATGTTGATTTTGATTGGTTTTTCAATAGCATCTTGGGCCcttat  >  2:2364144/1‑140 (MQ=255)

TACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCACTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTAT  >  NZ_CP025268/774033‑774305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`