BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	857,496	C→T	P20S (CCC→TCC)	CXP41_RS24120 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	857,496	0	C	T	98.4%	441.7 / ‑6.5	125	P20S (CCC→TCC)	CXP41_RS24120	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (63/58); minor base G (2/0); total (66/59)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACT  >  NZ_CP025268/857360‑857631

      tgaCAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAGGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGTCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAAt                                                                                                                                >  2:4454211/1‑140 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGat                                                                                                                         <  1:2278732/140‑1 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGat                                                                                                                         <  2:1400369/140‑1 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGat                                                                                                                         <  1:4366652/140‑1 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGat                                                                                                                         <  2:4371094/140‑1 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGat                                                                                                                         <  1:1742198/140‑1 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATCAAATGGCCCGTTAGACCTCACAAATCGCGAAGAGTTTCCCATTAATTTTTGat                                                                                                                         <  2:3152575/140‑1 (MQ=255)

             gAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGATGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTgct                                                                                                                         <  1:2140247/140‑3 (MQ=255)

                aaCATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGatata                                                                                                                      >  1:3393749/1‑140 (MQ=255)

                    tGCGTAATTCTTACGCAGTTCTCAGAAGAAGTGATTTAAATTTAGATGGATAGCAGTGTATGGAAACGTTCTGTTCCCTGGACTTGATCGTTAGACATCACAAATCGCGAAGCGTTTCCCATTAATCTTAGATATATTTa                                                                                                                  <  2:1073788/140‑1 (MQ=255)

                    tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                                                                                                                  <  2:1575578/140‑1 (MQ=255)

                    tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                                                                                                                  <  2:1430426/140‑1 (MQ=255)

                    tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                                                                                                                  <  2:144232/140‑1 (MQ=255)

                    tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                                                                                                                  <  1:4427668/140‑1 (MQ=255)

                    tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                                                                                                                  >  2:2583722/1‑140 (MQ=255)

                    tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCCGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                                                                                                                  <  2:2616583/140‑1 (MQ=255)

                      cGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATGACAAATCGCGAAGAGTTGCCCATTAATTTTTGATATATTTaaa                                                                                                                >  1:4646883/1‑140 (MQ=255)

                      cGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATGACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTaaa                                                                                                                >  1:4646840/1‑140 (MQ=255)

                      cGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTaaa                                                                                                                >  1:513432/1‑140 (MQ=255)

                         aaTACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACt                                                                                                             <  1:3192765/140‑1 (MQ=255)

                              ttACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGa                                                                                                        <  1:258434/140‑1 (MQ=255)

                                      ttCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTg                                                                                                >  1:4836090/1‑140 (MQ=255)

                                      ttCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTg                                                                                                >  1:4836305/1‑140 (MQ=255)

                                      ttCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTg                                                                                                >  1:3447469/1‑140 (MQ=255)

                                      ttCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTg                                                                                                >  1:3545994/1‑140 (MQ=255)

                                      ttCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTg                                                                                                >  1:3545995/1‑140 (MQ=255)

                                        gtctGAAAAAGTGAATTAAATTTAGATGGATAGCGGTGTATCGAAACGTTCTGTTACATGAAATGGCCCGTCAGACATGTCAAATCGCGAAGAGTTTCCCATTAATTTTGGATATATTTAAAACTTAGGACTTATGTGaa                                                                                              >  2:2042917/2‑140 (MQ=255)

                                        ctctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGTAAACGTTCTGTTACATGAAATGGTCCGTTAGACATCACAAATCGCGAAGAGTTTCCTATTAATTTTTGATATCTTTAAACCTTAGCACTTAATTGaa                                                                                              >  2:2042958/1‑140 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACTTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTCAAAACTTAGGACTTATTTGAAt                                                                                             <  2:4096580/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGTGTATCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  2:238523/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATCTTTAAAACTTAGGACTTATTTGAAt                                                                                             <  2:1595646/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  1:785694/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  1:783994/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  2:3394126/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  2:1186673/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  2:1310352/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGACAGTTAGACATCACCAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  2:1361638/140‑1 (MQ=255)

                                         tctGAAAAAGTGATTTAAATTTAGAGGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                             <  1:1180237/140‑1 (MQ=255)

                                           tGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATca                                                                                           <  2:4497672/140‑1 (MQ=255)

                                                aaGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCTCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCAAAttt                                                                                      >  2:161685/1‑140 (MQ=255)

                                                aaGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACATATTTGAATCACAgtt                                                                                      >  1:2843613/1‑140 (MQ=255)

                                                     aTTTACACTTAGAGGGATAGCGGTGTATGGAAACGTTCTGTTACACGAAATGGCCCGTTAGACGTCACAAATCGCGAAGAGTAGCCCGTTAATGTTTGATATATTTAAAACCTAGGGGTTATCTGACTCACCTATGAggt                                                                                 >  2:1981249/1‑140 (MQ=255)

                                                         acaTTTAGATGGATAGCGGTGTATGCAAACGTTCTGTTACATGAAATGGCCCGCTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATACTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGtt                                                                             <  1:3889344/138‑1 (MQ=255)

                                                                      tAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTg                                                                >  1:2146461/1‑140 (MQ=255)

                                                                          ggggtATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATg                                                            <  1:3280719/137‑1 (MQ=255)

                                                                          ggTGTATGGATACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGAGATATTTAAAACTTAGGACTTATTTGAATCCCATTTGAGGTGGTTATGAAAAAAATTGCATg                                                            <  1:1079797/140‑1 (MQ=255)

                                                                          ggTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATg                                                            <  1:2561610/140‑1 (MQ=255)

                                                                          ggTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATg                                                            <  2:4758208/140‑1 (MQ=255)

                                                                           gtgtATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGt                                                           >  1:1117810/1‑140 (MQ=255)

                                                                           gtgtATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGt                                                           >  1:3388034/1‑140 (MQ=255)

                                                                              tATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGACGAGTTTCCCATTAATTTTTGATATATTTAAAACTGAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCtt                                                        >  2:4510433/1‑140 (MQ=255)

                                                                              tATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCtt                                                        >  2:65184/1‑140 (MQ=255)

                                                                              tATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCtt                                                        >  1:1296554/1‑140 (MQ=255)

                                                                              tATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCtt                                                        >  2:4561684/1‑140 (MQ=255)

                                                                              tATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCtt                                                        >  1:1915435/1‑140 (MQ=255)

                                                                              tATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCtt                                                        >  2:2963715/1‑140 (MQ=255)

                                                                               aTGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCttt                                                       >  1:4578478/1‑140 (MQ=255)

                                                                                     aCGTTCTGATACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGCGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCAc                                                 >  2:650555/1‑140 (MQ=255)

                                                                                      cGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACt                                                >  1:4304056/1‑140 (MQ=255)

                                                                                      cGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACt                                                >  2:597179/1‑140 (MQ=255)

                                                                                      cGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACt                                                >  2:597706/1‑140 (MQ=255)

                                                                                      cGTTCTGTTACATAAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATGTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACt                                                >  2:2171455/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTCGACATCACCAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  2:1306721/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGGTATATTTAAGACTTAGGACTTATTTGAATGACATTCGAGGTGGTTATGAAAAAAATTGCCTGTCTTTCACCACTgg                                              >  2:4055974/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGaaa                                                                       <  1:375696/115‑1 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGaaa                                                                       >  2:375696/1‑115 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  2:2519368/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  1:4794782/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  2:2519705/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  2:2570647/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  1:3600226/1‑140 (MQ=255)

                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGATTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTgg                                              >  2:1032809/1‑140 (MQ=255)

                                                                                         tCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGc                                             <  1:2841454/140‑1 (MQ=255)

                                                                                          atGTTAAATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTAAGCACTGGcc                                            <  1:1675991/139‑1 (MQ=255)

                                                                                           tGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCg                                           <  1:1512543/140‑1 (MQ=255)

                                                                                             ttACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCa                                         >  1:1719872/1‑140 (MQ=255)

                                                                                                  tGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCt                                    >  2:778195/1‑140 (MQ=255)

                                                                                                  tGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCt                                    >  2:776807/1‑140 (MQ=255)

                                                                                                  tGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCt                                    >  2:775364/1‑140 (MQ=255)

                                                                                                  tGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCt                                    >  1:1536054/1‑140 (MQ=255)

                                                                                                   gAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTg                                   <  2:1119059/140‑1 (MQ=255)

                                                                                                     aaTGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCGTTCATCGTTGATATATTTACAACGTAGGACTTATTTGAATCACATTTGAGGGGGTTATGGAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGc                                 >  2:3617646/1‑140 (MQ=255)

                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCtt                               >  1:2186687/1‑140 (MQ=255)

                                                                                                          cccGTTAGACATCACAAATGGCGAAGAGTTTCCCATTAATTTTGGATATATTTAATACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAGAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCa                            >  2:2051888/1‑140 (MQ=255)

                                                                                                          cccGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCa                            >  1:148670/1‑140 (MQ=255)

                                                                                                            cGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCAcc                          <  1:4863496/140‑1 (MQ=255)

                                                                                                            cGTTAGACAACACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCAcc                          <  1:2273977/140‑1 (MQ=255)

                                                                                                             gTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCg                         <  1:4640266/140‑1 (MQ=255)

                                                                                                                 gACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAgg                     <  2:1638408/140‑1 (MQ=255)

                                                                                                                 gACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAgg                     <  1:3749929/140‑1 (MQ=255)

                                                                                                                 gACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAgg                     <  1:380825/140‑1 (MQ=255)

                                                                                                                 gACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAgg                     >  1:4139844/1‑140 (MQ=255)

                                                                                                                 gACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAgg                     <  2:1306771/140‑1 (MQ=255)

                                                                                                                           aTCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTa           >  2:3905795/1‑140 (MQ=255)

                                                                                                                           aTCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTa           >  2:3331313/1‑140 (MQ=255)

                                                                                                                             cgcgAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGc         >  2:1458800/1‑140 (MQ=255)

                                                                                                                              gcgAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCt        <  1:1647830/140‑1 (MQ=255)

                                                                                                                              gcgAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCt        <  1:1512878/140‑1 (MQ=255)

                                                                                                                                gAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGc      <  1:3206046/140‑1 (MQ=255)

                                                                                                                                gAAGAGTTTACCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATGGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGc      >  2:3079190/1‑140 (MQ=255)

GATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACT  >  NZ_CP025268/857360‑857631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`