BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	95,839	A→G	453 (TAA→TAG) I3V (ATT→GTT)	murF → mraY →	UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	95,839	0	A	G	99.7%	1195.0 / NA	354	453 (TAA→TAG) I3V (ATT→GTT)	murF mraY	UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (203/150); total (203/151)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

TGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATT  >  NZ_CP025268/95704‑95974

   tAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggct                                                                                                                                  >  2:4878062/1‑140 (MQ=255)

        cTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCg                                                                                                                             <  2:4580807/140‑1 (MQ=255)

            gTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                                                                                                                         >  1:4245601/1‑140 (MQ=255)

            gTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTGTACAGGAGAATGGGACATGTTAGTGTGGCTGGCCGAACa                                                                                                                         >  2:3270945/1‑140 (MQ=255)

              tAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAtt                                                                                                                       <  1:4904894/140‑1 (MQ=255)

              tAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAtt                                                                                                                       <  2:1185553/140‑1 (MQ=255)

                   acgactCTTAAATTACTGATTGCTGAGCAACAGCTAATTACGATTTTAGTTAAGGGTTCACGTACTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  2:4997980/135‑1 (MQ=255)

                   aCGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  1:3417737/140‑1 (MQ=255)

                   aCGCGTCTTAAATTACTGACTGCTGAGCAACAGGTCATGACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGGGGTAGTACGCGCTTTACAGGAGAATGGGAGATGTTAGTCTGGCTGGCCGAACATTTGGTc                                                                                                                  <  2:2595334/140‑1 (MQ=255)

                   aCGCGGCTTAAATTGCTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  1:3600778/140‑1 (MQ=255)

                          ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTTGCTGGCCGAACATTTGGTCAAAtatt                                                                                                           >  2:1553621/1‑140 (MQ=255)

                          ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatt                                                                                                           >  1:1917398/1‑140 (MQ=255)

                          ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatt                                                                                                           >  2:3197557/1‑140 (MQ=255)

                          ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatt                                                                                                           >  2:3890745/1‑140 (MQ=255)

                               ttACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTcc                                                                                                      <  1:5044043/140‑1 (MQ=255)

                                   tGATTGCTGAGCAACAGGTAATTACGATTTTGGTTAAGGGTTCACGTAGTGGCGCCATGGAAGAGGTAGTACGCGCTTGACAGGAGAATGGGACAGGTTAGTTTGGCTGGCCGAACATGTGGTCAAATATTATTCAgggt                                                                                                  >  1:1732612/1‑138 (MQ=255)

                                   tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt                                                                                                  >  1:3513641/1‑140 (MQ=255)

                                   tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt                                                                                                  >  1:1526898/1‑140 (MQ=255)

                                   tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt                                                                                                  >  1:206909/1‑140 (MQ=255)

                                     aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                                >  2:4737899/1‑140 (MQ=255)

                                      ttGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                               <  2:3529095/140‑1 (MQ=255)

                                       tGCTGAGCAACCGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTaa                                                                                              >  2:2517239/1‑140 (MQ=255)

                                          tGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGTGGTAGTACGCGCTTTACAGGAGAATTGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                           <  2:1483746/140‑1 (MQ=255)

                                            aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCt                                                                                         >  1:3726946/1‑140 (MQ=255)

                                                 cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  1:454677/1‑140 (MQ=255)

                                                 cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACAGGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  1:4072926/1‑140 (MQ=255)

                                                  aGGTAATTAAGAATGTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTCGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCt                                                                                   <  2:2883709/140‑1 (MQ=255)

                                                        ttACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTTTTCCGGCTTTAACGTTTTTTCCTATCTGa                                                                             >  2:4125578/1‑140 (MQ=255)

                                                        ttACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGa                                                                             >  1:557059/1‑140 (MQ=255)

                                                        ttACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGGTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGa                                                                             >  1:2938208/1‑140 (MQ=255)

                                                         tACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGAc                                                                            >  1:3679769/1‑140 (MQ=255)

                                                           cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGt                                                                          >  2:2369058/1‑140 (MQ=255)

                                                                 tAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGTACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                                    <  2:4172455/140‑1 (MQ=255)

                                                                  aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                                   >  1:1792878/1‑140 (MQ=255)

                                                                        gggTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGt                                                             >  2:3817115/1‑140 (MQ=255)

                                                                            tCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGc                                                         <  1:1615305/140‑1 (MQ=255)

                                                                            tCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGc                                                         >  1:1343159/1‑140 (MQ=255)

                                                                            tCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGc                                                         >  1:4450411/1‑140 (MQ=255)

                                                                            tCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGc                                                         >  2:4456978/1‑140 (MQ=255)

                                                                             cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGcc                                                        >  1:3034590/1‑140 (MQ=255)

                                                                             cACGTAGTGCAGCCATGGAAGAGGTAGTACGCGCTTTACCGGAGAATTGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTTTTTTCTTATCTGTCGTTTTGCGCCATGATCAGcg                                                        >  2:1303363/1‑139 (MQ=255)

                                                                                   gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                                                  <  1:4819373/140‑1 (MQ=255)

                                                                                   gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                                                  <  1:2538411/140‑1 (MQ=255)

                                                                                     gccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGAcc                                                >  1:675656/1‑140 (MQ=255)

                                                                                        gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  2:928941/140‑1 (MQ=255)

                                                                                        gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:2514486/140‑1 (MQ=255)

                                                                                        gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:3953646/140‑1 (MQ=255)

                                                                                        gccATGGAACAGGTAGTACCCGCTTTACAGGAGAATGGGACATGTTAGGTTGGCTGGCCGAACATTGGCTCGAATAGTATTCCGGGTTTACCGTCTTTGCCTATCTGCCGTTTCGCGCCATCGTCAGCCTGCGGACcgcg                                             >  2:623763/1‑140 (MQ=255)

                                                                                        gcaaTGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGTCGTTTCGCGCCATCGTCAGCCTGCTGCCcgcg                                             <  2:273746/137‑1 (MQ=255)

                                                                                         ccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCgcgc                                            >  1:624655/1‑140 (MQ=255)

                                                                                             ggAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGtt                                        <  2:2143407/140‑1 (MQ=255)

                                                                                              gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  2:1576961/1‑140 (MQ=255)

                                                                                                agagGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                                     <  2:860974/140‑1 (MQ=255)

                                                                                                agagGTAGTACGCGCTTTACAGGAGAATGGGAAATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                                     <  2:3057060/140‑1 (MQ=255)

                                                                                                   ggTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAtctc                                  >  1:3812467/1‑140 (MQ=255)

                                                                                                    gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa                                 <  2:1934046/140‑1 (MQ=255)

                                                                                                    gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa                                 >  1:1152042/1‑140 (MQ=255)

                                                                                                      agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               <  1:83852/140‑1 (MQ=255)

                                                                                                      agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               >  1:29126/1‑140 (MQ=255)

                                                                                                      agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               >  1:3133042/1‑140 (MQ=255)

                                                                                                       gtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTGTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtg                              >  2:1105607/1‑140 (MQ=255)

                                                                                                        taCGCGCTTTACAGGAGAATGGGACATGGTAGTTTGGCTGGCCGAACATTTGCGCAAATATTATTCCGGCTTTAATGTCTTTTCATATTTGACAAGTCGCGCCATCGTAAGCCTGCTGACAGTGCTGTTCATCTCATggt                             >  2:1845301/1‑140 (MQ=255)

                                                                                                           ccgcTTTACAGGAGTATGGGACATGTTAGTTTGGCTGGCCGAACACTCGGTCAAATATTATTCCGGCTGTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGACTGCTGTCCGCGCTGTTCATCTCATTGTGGa                          <  2:1986285/139‑1 (MQ=255)

                                                                                                            cgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt                         <  2:1710621/140‑1 (MQ=255)

                                                                                                            cgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt                         >  1:2141712/1‑140 (MQ=255)

                                                                                                            cgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt                         <  2:3293705/140‑1 (MQ=255)

                                                                                                            cgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt                         >  1:2601169/1‑140 (MQ=255)

                                                                                                             gcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATg                        >  2:3615816/1‑140 (MQ=255)

                                                                                                              cTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATgg                       >  1:1058717/1‑140 (MQ=255)

                                                                                                               tttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATggg                      >  1:3113977/1‑140 (MQ=255)

                                                                                                                ttACAGGAGAGTGGGACATGTTAGTTTGGCTGGCCGGACATTGGGTCAAATATTATTCCGGCTTGAACGTCTTTTCCTATCTGCCGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATGGTGGATGGGc                     <  2:379578/140‑1 (MQ=255)

                                                                                                                ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:1874827/140‑1 (MQ=255)

                                                                                                                ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:571603/140‑1 (MQ=255)

                                                                                                                ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:202066/140‑1 (MQ=255)

                                                                                                                ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:1053381/140‑1 (MQ=255)

                                                                                                                ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:65965/140‑1 (MQ=255)

                                                                                                                ttACAGGAGAATGCGATATGTTCGTTTGGCTGGCCGAACATTTGGTCAAATATTATTACGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:737912/140‑1 (MQ=255)

                                                                                                                ttACACGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCAGCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:333667/140‑1 (MQ=255)

                                                                                                                     ggAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCcgcg                <  1:2369058/140‑1 (MQ=255)

                                                                                                                     ggAGAATGGGACATGTTAGTTTCGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCcgcg                <  1:2847509/140‑1 (MQ=255)

                                                                                                                       agaATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTa              >  2:2055724/1‑140 (MQ=255)

                                                                                                                         aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCa                                                           <  2:1781807/93‑1 (MQ=255)

                                                                                                                         aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  2:5030510/1‑140 (MQ=255)

                                                                                                                         aaTGGGACATGTTAGGTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTGCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCGCATTGTTGAGGGGCCCGCGGATg            >  2:4341557/1‑140 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               >  2:2919900/1‑119 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     >  2:189158/1‑129 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  1:4331473/1‑140 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:2638888/1‑140 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:4265088/1‑140 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  1:2274853/1‑140 (MQ=255)

                                                                                                                           tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  1:2434772/1‑140 (MQ=255)

                                                                                                                           tGGGACAGGTTAGTTTGGCGGGCCGAACAGTTGGTCAAATATTATTCCGGCTGTGACGTCGTTTCCTATCTGACGTTTCGCGCCATCGTCATCCTGCTGACCGCGCTGTTCATCTCATTGTGGAGGGGCCCGCGTATGAt          >  2:3234459/1‑140 (MQ=255)

                                                                                                                             ggACATGGTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGACGTGAACGTCTTTTCCTATCTGACGTTTAGTGCCATCGTCAGCCTGCTGGCCGCGCTGATCATCTCATTGTATATGGGCCCGCGGATGACTg        >  2:4042712/1‑140 (MQ=255)

                                                                                                                                 aTGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCa    <  1:3847823/140‑1 (MQ=255)

                                                                                                                                   gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  2:3482490/1‑140 (MQ=255)

                                                                                                                                   gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  2:80168/1‑140 (MQ=255)

                                                                                                                                   gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  1:1290620/1‑140 (MQ=255)

                                                                                                                                   gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  2:4493685/1‑140 (MQ=255)

                                                                                                                                   gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  2:2968772/1‑140 (MQ=255)

                                                                                                                                   gTTAGTTTCGCTGGCCGGACATTTGGTCAAATATTATTCCGGCGTTAACGTCTTGTCCTATCTGACGTTTCGCTCCATCGTCAGCCTGCCGACCGCGCGGTTCATCTTATTATGGATGGGCGTGGGGGTGATTTCTCAtt  >  2:907236/1‑140 (MQ=255)

TGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATT  >  NZ_CP025268/95704‑95974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`