BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	895,066	A→G	179 (TAA→TAG)	rcdA →	DNA‑binding transcriptional regulator RcdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	895,066	0	A	G	100.0%	894.1 / NA	268	179 (TAA→TAG)	rcdA	DNA‑binding transcriptional regulator RcdA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (124/144); total (124/144)

Only 100 of 271 total aligned reads displayed.

CTCGAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAATAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCG  >  NZ_CP025268/894929‑895201

cTCGAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGCGGGGCTGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGgtagta                                                                                                                                       >  2:2365061/1‑140 (MQ=255)

cTCGAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGGTGCAGGgtagta                                                                                                                                       >  1:1711852/1‑140 (MQ=255)

   tagcAATCGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAGGCCGCTATCGCGCGAGGAGATTTTGAAGATGGTTGAGAGGGTTGCAGGGTAGTAGAt                                                                                                                                    <  2:256111/137‑1 (MQ=255)

   gAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGAt                                                                                                                                    <  2:1816832/140‑1 (MQ=255)

   gAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGAt                                                                                                                                    <  2:4983905/140‑1 (MQ=255)

   gAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGAt                                                                                                                                    <  2:4282573/140‑1 (MQ=255)

   gAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTCATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGCTTGAGAGGGTTGCAGGGTAGTAGAt                                                                                                                                    <  2:4576195/140‑1 (MQ=255)

         tGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGttt                                                                                                                              >  2:2464574/1‑140 (MQ=255)

             ttGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGa                                                                                                                          <  1:4809011/140‑1 (MQ=255)

             ttGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGa                                                                                                                          <  1:2132943/140‑1 (MQ=255)

              tGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGAt                                                                                                                         <  2:2194535/140‑1 (MQ=255)

                     ggAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGGTTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaa                                                                                                                  >  2:192427/1‑140 (MQ=255)

                     ggAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaa                                                                                                                  <  1:1084832/140‑1 (MQ=255)

                     ggAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaa                                                                                                                  <  2:1405085/140‑1 (MQ=255)

                     ggAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaa                                                                                                                  >  2:2773347/1‑140 (MQ=255)

                     ggAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaa                                                                                                                  <  1:2479184/140‑1 (MQ=255)

                     ggAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGCATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaa                                                                                                                  <  2:771056/140‑1 (MQ=255)

                      gAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACaaaaa                                                                                                                 >  1:3537430/1‑140 (MQ=255)

                           gcccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCa                                                                                                            >  2:1613744/1‑140 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCCCCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAATTTTAGATAACAAAAAACCCAt                                                                                                           >  2:2831794/1‑140 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCAt                                                                                                           <  1:3322919/140‑1 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCAt                                                                                                           >  1:3446349/1‑140 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCAt                                                                                                           <  2:2990201/140‑1 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCAt                                                                                                           <  2:297955/140‑1 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTCGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCAt                                                                                                           >  2:4803290/1‑140 (MQ=255)

                            cccgcGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCAGTTTGTCACCGACCGTACGCTGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCAt                                                                                                           >  2:2080956/1‑140 (MQ=255)

                                cgcgcTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGGAAGCCGCTATCGCGCGCGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTATATAACAAAAAACCCATCAAc                                                                                                       >  2:1463619/1‑140 (MQ=255)

                                           cGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGATGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGaa                                                                                            >  1:4209355/1‑140 (MQ=255)

                                             tttATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAAt                                                                                          >  2:357771/1‑140 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGATATgg                                                                                        <  2:4686459/140‑1 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATgg                                                                                        >  1:2373442/1‑140 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATgg                                                                                        <  2:2326736/140‑1 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATgg                                                                                        >  2:244751/1‑140 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATgg                                                                                        <  1:1337314/140‑1 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATgg                                                                                        >  1:678357/1‑140 (MQ=255)

                                               tATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCGATGGCGCTAGGAGATTTTTAGGATGGTTGAGAGTGTTGCAGGGTAGTAGATAAGTTTTATATAACAATAAACCCAGCAACCTTGAACCGAAATgg                                                                                        >  2:4124117/1‑140 (MQ=255)

                                               tATTGAGGGGATGACGCAGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATgg                                                                                        <  2:1978132/140‑1 (MQ=255)

                                                tttGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGc                                                                                       <  2:3041166/139‑1 (MQ=255)

                                                aTTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGc                                                                                       <  1:1956236/140‑1 (MQ=255)

                                                  tGAGGTGATGACGCTGAATTTTGTCTCCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTACATAAGTTTTATATAACAAAAAACCCATCAACCTTGAACCGAAATCGCgg                                                                                     <  2:2531085/140‑1 (MQ=255)

                                                  tGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCgg                                                                                     <  1:5035418/140‑1 (MQ=255)

                                                  tGAGGCGATGACGCTGCATGTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCgg                                                                                     <  1:3012156/140‑1 (MQ=255)

                                                      gggATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGtt                                                                                 >  1:303582/1‑140 (MQ=255)

                                                         aTGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGAGAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGCTGAt                                                                              >  1:4557597/1‑140 (MQ=255)

                                                          tGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATg                                                                             >  1:2187915/1‑140 (MQ=255)

                                                            aCGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATggg                                                                           <  1:1676368/140‑1 (MQ=255)

                                                            aCGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATggg                                                                           <  2:1131638/140‑1 (MQ=255)

                                                                 gCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCca                                                                      >  2:2709683/1‑140 (MQ=255)

                                                                  cATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCcac                                                                     >  2:389549/1‑140 (MQ=255)

                                                                  cATTTTGTCACCGACCGTAAGCCGCTATCGCGCCAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCcac                                                                     <  1:4902741/140‑1 (MQ=255)

                                                                       tGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAAt                                                                >  1:2124132/1‑140 (MQ=255)

                                                                       tGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAAt                                                                >  2:1478078/1‑140 (MQ=255)

                                                                          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggg                                                             <  2:1861476/140‑1 (MQ=255)

                                                                          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggg                                                             <  1:476135/140‑1 (MQ=255)

                                                                          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggg                                                             <  1:4139659/140‑1 (MQ=255)

                                                                          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTGTGAAGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggt                                                             <  2:4943589/140‑2 (MQ=255)

                                                                            ccgaccgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGa                                                           >  1:1883834/1‑140 (MQ=255)

                                                                               accgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACAt                                                        >  1:1186267/1‑140 (MQ=255)

                                                                                      gCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAGaa                                                 >  2:221076/1‑140 (MQ=255)

                                                                                       ccGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGaaa                                                <  1:1466522/140‑1 (MQ=255)

                                                                                          cTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGc                                             >  2:400394/1‑140 (MQ=255)

                                                                                           tATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCa                                            >  2:894624/1‑140 (MQ=255)

                                                                                             tCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGt                                          <  1:3194279/140‑1 (MQ=255)

                                                                                             tCGAGCGAGGAGATTTTGATGATGGATGAGAGGGTTGCAGAGTAGTAGATAAGTTCTAGCTAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGAGTTGATGGGATCCACAAAATGGGGACATCAAAGAAAAGCAGt                                          <  2:1762800/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAATTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  1:1373656/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  1:2283049/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  1:4982279/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  1:4451126/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  1:4381993/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  2:3767602/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGGTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  1:3067255/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGATTTAGACAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGCGTTGATGGGCTCCGCAAAATGGGGACATCAAAGAAAAGCAGTGTCa                                      <  2:1203512/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTTTGAGGATGGTCGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  2:424684/140‑1 (MQ=255)

                                                                                                 gcgAGGAGATTGTGAGGATGGTTGAGCGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                      <  2:647274/140‑1 (MQ=255)

                                                                                                    aggagATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATGAACCTTGAACCGAAATGGCGGAGTTGATGGGCTCCACAAAATGGGGACATCAATGAAGATCAGTgggatta                                   >  2:2682976/1‑135 (MQ=255)

                                                                                                     ggagATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTaa                                  >  1:2746320/1‑140 (MQ=255)

                                                                                                      gagaTTTTGAGGATGGTTGCGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAACACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAAt                                 <  1:2420699/140‑1 (MQ=255)

                                                                                                       agaTTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAAtt                                <  1:389549/140‑1 (MQ=255)

                                                                                                         aTTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATACGTTTTAGATAACAAAAAACCCATAAAACTTGAACCCAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTaa                              <  2:108239/140‑1 (MQ=255)

                                                                                                         aTTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTaa                              <  1:1116384/140‑1 (MQ=255)

                                                                                                         aTTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTaa                              <  2:3355993/140‑1 (MQ=255)

                                                                                                         aTTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAAACGAAATGGCGGGGTGGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATGaa                              <  2:4829540/140‑1 (MQ=255)

                                                                                                                 gATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATg                      >  1:1473990/1‑140 (MQ=255)

                                                                                                                   gggTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGGAAAGCAGTGGCACTAATTAAGACTGATGcc                    >  2:2399294/2‑140 (MQ=255)

                                                                                                                    ggTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGccc                   >  2:2027831/1‑140 (MQ=255)

                                                                                                                    ggTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGccc                   >  1:3982076/1‑140 (MQ=255)

                                                                                                                        gagaGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCg               <  1:2581016/140‑1 (MQ=255)

                                                                                                                           agGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAACCCCATCAACCTTGAACCGAAATGGCGTGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGaa            <  2:3395909/140‑1 (MQ=255)

                                                                                                                           agGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGaa            >  1:1922065/1‑140 (MQ=255)

                                                                                                                             ggTTGCAGGGGAGTAGATAAGTTGTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAAGTAAGACTGATGCCCTGCGGaaaa          >  2:4322699/1‑140 (MQ=255)

                                                                                                                               ttGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGt        >  1:868509/1‑140 (MQ=255)

                                                                                                                                ttcAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  2:733257/3‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGTCCTGCGGAAAAGtt       >  1:3988686/1‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  1:780966/1‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  1:4710208/1‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  1:1544107/1‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  2:5077278/1‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  1:2946778/1‑140 (MQ=255)

                                                                                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt       >  2:3335225/1‑140 (MQ=255)

                                                                                                                                tGCAGGGGAGTAGATAAGTTTTACATAAGAAAAAACACATCAACCTTGAACCGAAATGGCGGGCATGATAGGCCCCACACAATGGGGACATCAAAGAAAAGCAGTAGCACTAATGAAGACTGAGGGGCTGAGGAAAAGtt       >  2:2158911/1‑140 (MQ=255)

CTCGAACAATGGTTTGAACCCGGAACCGCCCGCGCGCTTGATGCGTTTATTGAGGGGATGACGCTGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAATAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCG  >  NZ_CP025268/894929‑895201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`