BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	920,456	A→G	L156L (TTG→CTG)	hcp ←	hydroxylamine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	920,456	0	A	G	99.2%	877.0 / NA	267	L156L (TTG→CTG)	hcp	hydroxylamine reductase
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (181/82); minor base C (1/1); total (182/85)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TCAAGAAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACG  >  NZ_CP025268/920317‑920592

        cgcgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCAcagca                                                                                                                                  >  1:3810020/1‑140 (MQ=255)

        cgcgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCAcagca                                                                                                                                  >  1:1362949/1‑140 (MQ=255)

        cgcgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCAcagca                                                                                                                                  >  1:2485830/1‑140 (MQ=255)

          cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTAGTGGGCATAAATATCGTTGTCGTATTGACCGAGGACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGGAcagcggg                                                                                                                                >  2:1633071/1‑137 (MQ=255)

          cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGTGCATAAATAGCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  1:2190656/1‑140 (MQ=255)

          cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  1:4824605/1‑140 (MQ=255)

          cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  1:763289/1‑140 (MQ=255)

            ttCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACg                                                                                                                              >  1:282178/1‑140 (MQ=255)

            ttCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACg                                                                                                                              <  1:4963730/140‑1 (MQ=255)

                tatCGGAAGGCCAGGTCCCCAGCCAGGCCATGATTTTATGGTACTGGGCATAAATACCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAgg                                                                                                                          <  2:4031883/140‑1 (MQ=255)

                 atCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGc                                                                                                                         >  1:4397472/1‑140 (MQ=255)

                    ggCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCga                                                                                                                      >  1:1858305/1‑140 (MQ=255)

                      cAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgaga                                                                                                                    <  2:610486/140‑1 (MQ=255)

                      cAGGCCAGGGCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgaga                                                                                                                    <  2:1508510/140‑1 (MQ=255)

                         gCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATg                                                                                                                 >  1:4601326/1‑140 (MQ=255)

                         gCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATg                                                                                                                 >  1:1319678/1‑140 (MQ=255)

                           cAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtt                                                                                                               >  2:1512554/1‑140 (MQ=255)

                             ggTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtttt                                                                                                             >  2:4277540/1‑140 (MQ=255)

                                   cAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGTCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCaa                                                                                                       >  1:2098809/1‑140 (MQ=255)

                                   cAGCCACGCCATGATTTTATGGTACTAGGCTTAAATATCGTTGTCGTATTGCCCGAGAACATGCGCGTGTTCGATGTAGGCCGCCGCACCTTTAAGGACATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCaa                                                                                                       >  1:3055598/1‑140 (MQ=255)

                                    aGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGCGAATGTTTTCGCCact                                                                                                      >  2:4177430/1‑138 (MQ=255)

                                     gCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATCGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                                     <  2:3687338/140‑1 (MQ=255)

                                     gCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                                     <  1:1126909/140‑1 (MQ=255)

                                      ccACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                    <  2:4869476/140‑1 (MQ=255)

                                       cACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgc                                                                                                   <  2:3556835/140‑1 (MQ=255)

                                        aCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgcc                                                                                                  >  1:4764916/1‑140 (MQ=255)

                                         cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATCGGCCGCCGCACCTGTCCGGCCATACAGGCACAGCAGACGCAGGCCGAGCATGTTTTCGCCAATcgccg                                                                                                 >  2:832497/1‑140 (MQ=255)

                                         cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                 >  1:235391/1‑140 (MQ=255)

                                         cGCCATGATTTTATGGTACTGGGCATAAATATCGTGGTCGTATTGACCGAGAACATGCGCGTGTTCCATATATGCCGCCGCACTTTTCAGGCCATACAGGCACGGCAGACGCAGGCAGAGAATGTTTTCGCCGATcgcgg                                                                                                 >  2:4551555/1‑138 (MQ=255)

                                              tGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTa                                                                                            >  2:5082818/1‑140 (MQ=255)

                                                     aTGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                     <  1:89093/140‑1 (MQ=255)

                                                       ggTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                                   <  1:2962815/140‑1 (MQ=255)

                                                       ggTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGCTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                                   <  2:4077749/140‑1 (MQ=255)

                                                          aCTGGGCATAAGTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTGCAAGCGATACAGGCACAGCAGACGCATGCCGAGGATGTTTGCGCCAATCGCCGCGTTATCTTTGTTAggg                                                                                >  2:3870623/1‑140 (MQ=255)

                                                          aCTGGGCATAACTAGCGTGGTCGTATTGACCGCGCACATGCGCGGGTTCCATATGGGCCGCCGCACCTTTCAGGCTATACAGGCACAGCAGGCGCAGGCCGAGAATGTTTTCGCCTCTCGCCGCTTTATCTTGGTTAggg                                                                                >  2:1998246/1‑140 (MQ=255)

                                                          aCTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                                >  1:3978255/1‑140 (MQ=255)

                                                          aCTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                                >  1:4032929/1‑140 (MQ=255)

                                                           cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATGGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGGCAATCGCCGCGTCATAGTTGCTAgggg                                                                               >  2:2962793/1‑140 (MQ=255)

                                                           cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGCGTTCCATATAGGCCGCCTCACCTTTCAGGCCAAACAGTCACAGAAGCCGCTGGCCGAGTATGGTCTCGCCAATCGCCGCGTTATCTTTGTTAggcg                                                                               >  2:300200/1‑138 (MQ=255)

                                                            tGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGt                                                                              <  1:677982/140‑1 (MQ=255)

                                                              ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:4262111/1‑140 (MQ=255)

                                                              ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:96110/1‑140 (MQ=255)

                                                              ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  2:1644129/1‑140 (MQ=255)

                                                              ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:2212931/1‑140 (MQ=255)

                                                                   aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  2:3135978/1‑140 (MQ=255)

                                                                   aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  1:5029877/1‑140 (MQ=255)

                                                                   aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTGTATCTTTGTTAGGTGTAAATTct                                                                       >  2:187960/1‑140 (MQ=255)

                                                                        tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCtgctgc                                                                  >  1:4520986/1‑140 (MQ=255)

                                                                        tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCtgctgc                                                                  >  2:1064907/1‑140 (MQ=255)

                                                                        tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCtgctgc                                                                  >  2:122818/1‑140 (MQ=255)

                                                                        tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCtgctgc                                                                  >  1:1251576/1‑140 (MQ=255)

                                                                         cGTTGTCGTATTGACCGAGAACATGCGCGTTTTCCATATAGGCCTCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGTGGTAAATTCTGCTGcc                                                                 <  2:1099360/140‑1 (MQ=255)

                                                                         cGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 >  1:2709088/1‑140 (MQ=255)

                                                                         cGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 <  2:4957212/140‑1 (MQ=255)

                                                                         cGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTAGCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 >  2:1792705/1‑140 (MQ=255)

                                                                             gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGAc                                                             >  2:2790085/1‑140 (MQ=255)

                                                                                  aTTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGc                                                        <  1:2128116/140‑1 (MQ=255)

                                                                                          agaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCATACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGcc                                                >  2:2226835/1‑140 (MQ=255)

                                                                                               aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAt                                           <  1:3259164/140‑1 (MQ=255)

                                                                                               aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTGTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCGCGCCGAGAt                                           <  2:3846144/140‑1 (MQ=255)

                                                                                               aTGCGCCTGTTCCTTATCCGCCGCCACACCTTTCAGACCATCCAATCACAGCAGGCGCAGGCTGAGTATGTTTTCGCGAATCGCCGCTTTATCTTTGTTAGGATTAAAATCTTCTGCCCGTCGATGCAGCTCACCGAGAt                                           <  2:3269481/140‑1 (MQ=255)

                                                                                                tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtc                                          >  2:1235598/1‑140 (MQ=255)

                                                                                                tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtc                                          >  1:2407336/1‑140 (MQ=255)

                                                                                                     gtgtTACATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCAAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGc                                     <  1:2115606/140‑1 (MQ=255)

                                                                                                      tgtTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCt                                    >  1:172719/1‑140 (MQ=255)

                                                                                                      tgtTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCt                                    >  1:3388876/1‑140 (MQ=255)

                                                                                                       gtTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTc                                   <  1:3341977/140‑1 (MQ=255)

                                                                                                         tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  1:3402169/140‑1 (MQ=255)

                                                                                                         tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  1:289066/140‑1 (MQ=255)

                                                                                                         tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  1:3290387/140‑1 (MQ=255)

                                                                                                          ccATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAcc                                <  2:1347188/140‑1 (MQ=255)

                                                                                                                 ggCCGCCGCACCTTTCCGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAAGCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa                         <  1:4214785/140‑1 (MQ=255)

                                                                                                                  gtcgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACTTTGCAGCTCGCCGAGATCGACGCTCACCAGTTGCAg                        >  1:2849616/3‑140 (MQ=255)

                                                                                                                  gccgccGCACCTTTCAGGCCATACAGGCATCGCAGACGCAGGCCGAGAATGTGTTCGCCAATCGCCGCGTTATCTTTGTTAGGGGTAAATTCTGCAGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTGACCAGGTGCAg                        >  2:2629609/1‑140 (MQ=255)

                                                                                                                  gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCTGAGGAGGTTTTCGCCATTCACCGCTTTACCTTTGTTAGGGGTAAATACTGCGGCCTGACGTTGCAGCTCGGCGAGATCGGCGCGCACCAGTTGCAg                        >  2:3455600/1‑140 (MQ=255)

                                                                                                                  gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:2577145/1‑140 (MQ=255)

                                                                                                                  gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:4095031/1‑140 (MQ=255)

                                                                                                                  gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:3642455/1‑140 (MQ=255)

                                                                                                                   ccgccgCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAgg                       >  1:521925/1‑140 (MQ=255)

                                                                                                                    cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      <  1:3461024/140‑1 (MQ=255)

                                                                                                                    cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      <  1:1155849/140‑1 (MQ=255)

                                                                                                                    cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      <  1:2008639/140‑1 (MQ=255)

                                                                                                                     gccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGTGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTc                     >  1:4064246/1‑140 (MQ=255)

                                                                                                                     gccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTc                     >  1:1773045/1‑140 (MQ=255)

                                                                                                                     gccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCGGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTc                     >  1:4653772/1‑140 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGGAGACGCAGGCCGAGAATGTTATCGCCAATCGCCGCTTTATCTTTGATAGGGGTAAGCTCTGCTGCCTGACGTTGCAGTTCGCCGAGATCGGCGCTCACCAGATGCAGGTCAg                   >  2:3730304/1‑140 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                     <  2:4619780/74‑1 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 <  2:4704735/94‑1 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 >  1:4704735/1‑94 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:3536418/1‑140 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:3529197/1‑140 (MQ=255)

                                                                                                                       cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:4489130/1‑140 (MQ=255)

                                                                                                                         cACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGcc                 >  1:1067397/1‑140 (MQ=255)

                                                                                                                           ccTTTCAGGCCATACAGGCACAGCAGACGGGGGCCGAGAATGTTTGCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTAGCTCACCAGTTGCAGGGCAGCCAt               <  1:1495251/140‑1 (MQ=255)

                                                                                                                           ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  2:203016/140‑1 (MQ=255)

                                                                                                                            cTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATc              <  2:726092/140‑1 (MQ=255)

                                                                                                                             tttCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCg             >  1:2475367/1‑140 (MQ=255)

                                                                                                                              ttCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCgg            >  1:1555312/1‑140 (MQ=255)

                                                                                                                              ttCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCgg            >  1:2740362/1‑140 (MQ=255)

                                                                                                                                      aTACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGa    >  1:5008849/1‑140 (MQ=255)

TCAAGAAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACG  >  NZ_CP025268/920317‑920592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`