BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,075,358	A→G	58 (TAA→TAG)	ymdF →	general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,075,358	0	A	G	99.6%	753.1 / NA	231	58 (TAA→TAG)	ymdF	general stress protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (95/134); minor base T (0/1); total (95/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACG‑AAT‑CA‑TTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075224‑1075491

gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  1:1164150/140‑1 (MQ=255)

gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  1:461411/140‑1 (MQ=255)

gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  2:1208138/140‑1 (MQ=255)

gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  2:1496250/140‑1 (MQ=255)

gaCCGCGAAAGAGCATCAGAAGCAGATAAAAAACGTGGACAGCAGAGCGGGGGTAATTTCAAGAAGGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  2:1144310/140‑1 (MQ=255)

   cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGGAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  2:3184061/1‑140 (MQ=255)

     cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                                                                                                                 >  1:400823/1‑140 (MQ=255)

             cATCAGAAGCAGGTAAAAAAGGCGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCACCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACCAGCCGGGCTAATCAATGAca                                                                                                                         >  2:1112409/1‑139 (MQ=255)

             cATCAGAAGCAGATAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACg                                                                                                                         >  2:5030739/1‑140 (MQ=255)

                cAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGTTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AAt                                                                                                                     <  2:3648988/140‑1 (MQ=255)

                cAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AAt                                                                                                                     <  1:1968082/140‑1 (MQ=255)

                cAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AAt                                                                                                                     >  1:2152146/1‑140 (MQ=255)

                     gCAGGGAAAAAAGGCGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGCGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AAtgcatt                                                                                                                <  2:835665/140‑6 (MQ=255)

                      cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGCGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑ttt                                                                                                              <  1:2192985/140‑1 (MQ=255)

                      cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACACGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGTCTAATCAATGACG‑AATGCA‑ttt                                                                                                              >  2:4015590/1‑140 (MQ=255)

                        ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑ttttt                                                                                                            >  2:2659188/1‑140 (MQ=255)

                        ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑ttttt                                                                                                            >  1:4979262/1‑140 (MQ=255)

                                 ggTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGc                                                                                                   <  2:984307/140‑1 (MQ=255)

                                      acagcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTc                                                                                              <  2:3698811/140‑1 (MQ=255)

                                          cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCaaaa                                                                                          >  2:1731755/1‑140 (MQ=255)

                                            cagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAg                                                                                        <  2:4979262/140‑1 (MQ=255)

                                               cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCa                                                                                     >  1:315517/1‑140 (MQ=255)

                                                 ggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATc                                                                                   >  1:3981793/1‑140 (MQ=255)

                                                  gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCa                                                                                  <  1:4167291/140‑1 (MQ=255)

                                                    gTAATTTCAAGAATGCCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                <  1:3000681/140‑1 (MQ=255)

                                                    gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:5011238/1‑140 (MQ=255)

                                                    gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                <  1:4588136/140‑1 (MQ=255)

                                                    gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  2:2558775/1‑140 (MQ=255)

                                                    gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  2:4803111/1‑140 (MQ=255)

                                                    gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:5001209/1‑140 (MQ=255)

                                                      aaTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccgc                                                                              <  1:4497792/140‑1 (MQ=255)

                                                         ttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                           <  1:1576066/140‑1 (MQ=255)

                                                          tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                          <  2:4931413/140‑1 (MQ=255)

                                                          tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                          <  1:1343922/140‑1 (MQ=255)

                                                          tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                          <  2:2918256/140‑1 (MQ=255)

                                                            aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTa                                                                        <  2:1662665/140‑1 (MQ=255)

                                                              gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  2:3460953/140‑1 (MQ=255)

                                                              gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  2:774189/140‑1 (MQ=255)

                                                              gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      >  1:5060696/1‑140 (MQ=255)

                                                              gAATGACCACCAGCCCGCATCTGAAGCAGGTAAAAAAGGTCGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGAGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  2:1447204/140‑1 (MQ=255)

                                                                aTGACCCGCAGCGCGCATCTGAAGCATGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATACA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:4014685/140‑1 (MQ=255)

                                                                aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:1456735/140‑1 (MQ=255)

                                                                aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:1953325/140‑1 (MQ=255)

                                                                aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:4894800/140‑1 (MQ=255)

                                                                   aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACACCTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  1:3722292/1‑140 (MQ=255)

                                                                   aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  2:1592897/1‑140 (MQ=255)

                                                                        cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGa                                                            >  1:1212532/1‑140 (MQ=255)

                                                                         aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGcc                                                                                      <  2:1695712/113‑1 (MQ=255)

                                                                         aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGcc                                                                                      >  1:1695712/1‑113 (MQ=255)

                                                                           cgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATAc                                                         >  1:1028828/1‑140 (MQ=255)

                                                                             cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                       <  1:977667/140‑1 (MQ=255)

                                                                                  cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:618560/140‑1 (MQ=255)

                                                                                  cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAAATACCCGCGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCGCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:3635512/140‑1 (MQ=255)

                                                                                      aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  1:702076/140‑1 (MQ=255)

                                                                                       gCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATg                                             <  1:3224895/140‑1 (MQ=255)

                                                                                       gCAGGTAAAAAAGGTGCTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGCCG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGCTACTGATGACAAATg                                             <  2:2005686/140‑1 (MQ=255)

                                                                                         aGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACC‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTa                                           <  2:4141336/140‑1 (MQ=255)

                                                                                          ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  2:562386/1‑140 (MQ=255)

                                                                                          ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:1837575/1‑140 (MQ=255)

                                                                                           gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  1:3812887/140‑1 (MQ=255)

                                                                                            tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGc                                        >  1:1604825/1‑140 (MQ=255)

                                                                                                aaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGc                                    <  1:989540/140‑1 (MQ=255)

                                                                                                   ggtggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                 <  2:2795039/140‑1 (MQ=255)

                                                                                                   ggtggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                 <  1:2555388/140‑1 (MQ=255)

                                                                                                      ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              >  1:2871556/1‑140 (MQ=255)

                                                                                                       gtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgc                             >  2:2501121/1‑140 (MQ=255)

                                                                                                        tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACT‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCAGCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:4337041/140‑1 (MQ=255)

                                                                                                        tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:3879454/140‑1 (MQ=255)

                                                                                                        tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:1489362/140‑1 (MQ=255)

                                                                                                        aaaGAGCAGTCACGGCAAAATCGCCACCTCGCCGGGATAAGCTATCACA‑ACTGCA‑CTTTTGATTTTCGCTCGTCAAAAAGCCATCTCCGCAAGTTCCCCGGTGGTTTATACTAATGACAAATGTAAGCTTGCCGGATgcg                            <  2:4214190/139‑1 (MQ=255)

                                                                                                        aaaGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:2057413/139‑1 (MQ=255)

                                                                                                          agagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                          <  2:2273932/140‑1 (MQ=255)

                                                                                                          agagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                          <  1:486994/140‑1 (MQ=255)

                                                                                                          agagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                          <  2:879105/140‑1 (MQ=255)

                                                                                                          agagCAGTCACGGCAAAAGCGACAACTAGCCGGGCCAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGcgcg                          <  1:427578/140‑1 (MQ=255)

                                                                                                           gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  2:3841574/140‑1 (MQ=255)

                                                                                                           gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  1:2434284/140‑1 (MQ=255)

                                                                                                           gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  1:3570254/140‑1 (MQ=255)

                                                                                                            agCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGAt                        <  1:3791769/140‑1 (MQ=255)

                                                                                                             gCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                       <  2:3044490/140‑1 (MQ=255)

                                                                                                                 tCACGGCAAAAGCGACAACTAGCCGGGTTAATCAATGACT‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCACTGGTTACCCGGTGGGTGATACTGGTGACAGATGTAAGCTGGCCTGATGCGCTATGCTTa                   >  2:3900987/1‑140 (MQ=255)

                                                                                                                 tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   >  1:702552/1‑140 (MQ=255)

                                                                                                                 tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   <  1:2694697/140‑1 (MQ=255)

                                                                                                                 tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   <  1:2369348/140‑1 (MQ=255)

                                                                                                                  cACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                  <  1:514160/140‑1 (MQ=255)

                                                                                                                   aCGGCCAAATCGACAATTAGCCAGGGTAATCACTCACGTATT‑CATTTTTTT‑CTGTGTCTCGTCAAAAAGCCATCACCGCCGGTTTTTCGGTGGTTGATACTGATGATTACCTTATTCTTGCCTACTTCCCGATGCTTATc                 <  2:465361/140‑1 (MQ=255)

                                                                                                                      gCAAAAGCGACAGGTAGCCGGGCGCATCAATGACG‑AATGCG‑GTTGTGTCTGTGGCTCGGCAAAGAGCCAGCACCGCCGGTTACCCGGTGGTTGATAGTGATGACAAAGGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  2:453529/140‑1 (MQ=255)

                                                                                                                      gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                  <  1:4290437/136‑1 (MQ=255)

                                                                                                                      gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                  >  2:4290132/1‑136 (MQ=255)

                                                                                                                      gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:851384/140‑1 (MQ=255)

                                                                                                                      gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              >  2:3293667/1‑140 (MQ=255)

                                                                                                                      gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:695568/140‑1 (MQ=255)

                                                                                                                      gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              >  1:602092/1‑140 (MQ=255)

                                                                                                                         aaaGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGACCt           >  2:1254231/1‑140 (MQ=255)

                                                                                                                           aGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         <  2:948570/140‑1 (MQ=255)

                                                                                                                                cAACTAGCCGGGCTAATCCATGTCG‑AATGCG‑TTTTTGTCGGGAGCTCGTCAAAGAGCCACCACCGCGGGTTCCCCGGTGGTTGATACTTCTTACAAATGTAAGCTTGCCTGATGCGCGATGCTGATCAGGCCTACCagac    <  2:2981259/140‑2 (MQ=255)

                                                                                                                                cAACTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGTGCGATGCTTATCAGGCCTACCagaa    <  1:1928220/140‑1 (MQ=255)

                                                                                                                                  aCTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  1:4736335/140‑1 (MQ=255)

                                                                                                                                  aCTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  1:1694189/140‑1 (MQ=255)

                                                                                                                                  aCTAGCCGGGCTAATCAATGACG‑AATGCA‑TTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  2:4346602/140‑1 (MQ=255)

GACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACG‑AAT‑CA‑TTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075224‑1075491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`