Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,156,813 A→G *318* (TAA→TAG)  fabH → beta‑ketoacyl‑ACP synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,156,8130AG99.7% 964.5 / NA 290*318* (TAA→TAG) fabHbeta‑ketoacyl‑ACP synthase III
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (186/103);  total (187/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGG  >  NZ_CP025268/1156677‑1156949
                                                                                                                                        |                                                                                                                                        
gTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGAt                                                                                                                                       >  1:746764/1‑140 (MQ=255)
 tAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATa                                                                                                                                      <  1:112040/140‑1 (MQ=255)
 tAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATa                                                                                                                                      <  2:3378168/140‑1 (MQ=255)
  aaTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCGGCGCTGGTTCGTTTCTAGGATaa                                                                                                                                     >  2:2416884/1‑140 (MQ=255)
  aaTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCGTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATaa                                                                                                                                     >  2:4090571/1‑140 (MQ=255)
   aTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAg                                                                                                                                    >  1:2755175/1‑140 (MQ=255)
      ccTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGAt                                                                                                                                 >  1:5058741/1‑140 (MQ=255)
        tctGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTa                                                                                                                               <  1:1484646/140‑1 (MQ=255)
         ctGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaa                                                                                                                              >  1:4737836/1‑140 (MQ=255)
         ctGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaa                                                                                                                              >  1:4301932/1‑140 (MQ=255)
         ctGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaa                                                                                                                              >  1:1941041/1‑140 (MQ=255)
          tGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCCGTACGCGACGGGCGCATTAAGCCGGGGCAGGTGGATCTGCTTGAAGTCTTTGGCGGTGGATGCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaaa                                                                                                                             >  2:2114859/1‑140 (MQ=255)
           gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATa                                                                                                                                      >  1:1029919/1‑130 (MQ=255)
           gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATa                                                                                                                                      <  2:1029919/130‑1 (MQ=255)
           gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaaaa                                                                                                                            >  2:4969454/1‑140 (MQ=255)
           gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaaaa                                                                                                                            >  1:4166667/1‑140 (MQ=255)
           gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaaaa                                                                                                                            >  1:4076140/1‑140 (MQ=255)
           gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTaaaa                                                                                                                            >  2:1914093/1‑140 (MQ=255)
             ggCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACa                                                                                                                          <  2:2849244/140‑1 (MQ=255)
                ctctGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGa                                                                                                                       >  1:4851727/1‑140 (MQ=255)
                 tctGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGAc                                                                                                                      >  1:3668822/1‑140 (MQ=255)
                      cccGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAAt                                                                                                                 <  1:2073755/140‑1 (MQ=255)
                         gTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTg                                                                                                              >  2:2539310/1‑140 (MQ=255)
                         gTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTg                                                                                                              >  1:4218683/1‑140 (MQ=255)
                         gTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTg                                                                                                              <  2:1726474/140‑1 (MQ=255)
                         gTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTg                                                                                                              <  1:3561804/140‑1 (MQ=255)
                          tGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGTATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGc                                                                                                             >  2:4260270/1‑140 (MQ=255)
                          tGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGc                                                                                                             >  2:2687227/1‑140 (MQ=255)
                             gcgcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCAtt                                                                                                          >  1:3927931/1‑140 (MQ=255)
                               gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATa                                                                                                                                      <  1:1620670/110‑1 (MQ=255)
                               gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAAt                                                                                                                 >  2:3794762/1‑131 (MQ=255)
                               gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAAt                                                                                                                 <  1:3794762/131‑1 (MQ=255)
                               gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCAtttt                                                                                                        >  2:556652/1‑139 (MQ=255)
                                cTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                       <  1:570792/140‑1 (MQ=255)
                                cTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                       <  1:3703612/140‑1 (MQ=255)
                                cTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                       <  1:340288/140‑1 (MQ=255)
                                cTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                                                       <  1:2321697/140‑1 (MQ=255)
                                cTGGATGAAGCTGGACGCGACGGGCGCATGAGGCCTGGGCAGTTGGTTCTGCTTGTAGCCTTTGACGGTGGATTAACCTGGAGCTCCGCGCTGGTTCGTTGCTAGGATAAGGATTAGAGCATGACGCAATTTGCATTtgt                                                                                                       <  1:2564084/140‑1 (MQ=255)
                                     tGAAGCTGTACGCGACGGGCGAATTAGGGCGTGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTGACCTGGGGCTCCGCGTGGGTTCGTTTCTAGGATAAGAATTAAAACGTGACGCAAGTTGCATTTGTGTTcc                                                                                                  <  2:2307108/140‑1 (MQ=255)
                                         gCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTgg                                                                                              >  2:452471/1‑140 (MQ=255)
                                           tGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGAc                                                                                            >  1:3194885/1‑140 (MQ=255)
                                            gTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACa                                                                                           <  2:1301214/140‑1 (MQ=255)
                                             tACGGGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          >  2:4678849/1‑140 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGGCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          >  1:4346633/1‑140 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          >  1:1571684/1‑140 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          >  2:2948762/1‑140 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          <  2:5063591/140‑1 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          >  1:1796258/1‑140 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg                                                                                          >  2:3034487/1‑140 (MQ=255)
                                             tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGGGGATTCGCCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAGAACATGACGCACTTTGCATTTGTGTTCCCTGGACAg                                                                                          >  2:861744/1‑140 (MQ=255)
                                              aCGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAgg                                                                                         <  2:4400724/140‑1 (MQ=255)
                                               cgcgACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAggg                                                                                        >  1:5047384/1‑140 (MQ=255)
                                                  gACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTtc                                                                                     >  1:4915810/1‑140 (MQ=255)
                                                    cGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTtctc                                                                                   >  1:4454889/1‑140 (MQ=255)
                                                    cGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTtctc                                                                                   >  1:3304433/1‑140 (MQ=255)
                                                     gggCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCa                                                                                  <  1:1314867/140‑1 (MQ=255)
                                                       gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCaaa                                                                                >  1:1997629/1‑140 (MQ=255)
                                                       gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCaaa                                                                                >  1:2414468/1‑140 (MQ=255)
                                                       gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCaaa                                                                                >  2:2471315/1‑140 (MQ=255)
                                                       gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCaaa                                                                                >  1:1522235/1‑140 (MQ=255)
                                                            ttAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGtt                                                                           <  1:1652696/140‑1 (MQ=255)
                                                             tAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTg                                                                          <  1:624624/140‑1 (MQ=255)
                                                                 ccGGGGCAGTTGGTTCTGCTTGAAGCGTTTCGCGGTGGATTCACCTGGGGCGCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGGCGGAATTTGCAGTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAAt                                                                      >  1:3051098/1‑140 (MQ=255)
                                                                 ccGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAAt                                                                      >  1:1443829/1‑140 (MQ=255)
                                                                  cGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATg                                                                     <  1:4942317/140‑1 (MQ=255)
                                                                  cGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATg                                                                     <  2:3912305/140‑1 (MQ=255)
                                                                  cGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATg                                                                     <  2:1443829/140‑1 (MQ=255)
                                                                    gggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgct                                                                   <  2:1592309/140‑1 (MQ=255)
                                                                     ggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                                  >  2:245937/1‑140 (MQ=255)
                                                                     ggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                                  >  1:4023818/1‑140 (MQ=255)
                                                                     ggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                                  >  2:4820144/1‑140 (MQ=255)
                                                                     ggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                                  >  1:24599/1‑140 (MQ=255)
                                                                     ggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                                  >  2:1794404/1‑140 (MQ=255)
                                                                     ggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATgctg                                                                  >  2:3610471/1‑140 (MQ=255)
                                                                     ggCAGTTGGTTCGGCTTGAAGCCTGTGGCGGTGGATTCACCTGGGGCTGCGCGCTGGTGCGTGGCTAGGATGAGGATTAAAACATGACGCAGGTTGCGTTTGTGTTCTTGTGACAGGGTTCTCAAACCGTTGGGATgctg                                                                  >  2:3148042/1‑140 (MQ=255)
                                                                       cAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGAGTGGCCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCAGTTGGGTTCCCGGGGCAGGGTTGTCAAACCGTTGGAATgctggc                                                                <  2:4641930/140‑1 (MQ=255)
                                                                                 tGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcg                                                      <  1:2407117/140‑1 (MQ=255)
                                                                                   cTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcggc                                                    >  1:1931993/1‑140 (MQ=255)
                                                                                      gAAGCCTTTGGCGGTGGATTCACCTGCGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCAGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAg                                                 <  2:4010810/140‑1 (MQ=255)
                                                                                        aGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCt                                               >  1:4584571/1‑140 (MQ=255)
                                                                                           cTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATc                                            >  1:3225531/1‑140 (MQ=255)
                                                                                             ttGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCa                                          >  1:951483/1‑140 (MQ=255)
                                                                                              tGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCaa                                         >  1:2371246/1‑140 (MQ=255)
                                                                                                  ggTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTGCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGt                                     <  2:752729/140‑1 (MQ=255)
                                                                                                           aCCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGCATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAAc                            <  2:2708678/140‑1 (MQ=255)
                                                                                                             cTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGt                          <  2:4147833/140‑1 (MQ=255)
                                                                                                                gggCTCCGCGCTGGTTCGTTTTTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTGAAACCGATGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTGGAAGAAATGTTTg                       >  2:2658709/1‑140 (MQ=255)
                                                                                                                gggCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTg                       >  1:2611198/1‑140 (MQ=255)
                                                                                                                  gCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCt                     >  2:1248297/1‑140 (MQ=255)
                                                                                                                     ccGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGaa                  >  1:3854020/1‑140 (MQ=255)
                                                                                                                      cgcgCTGGTTCGTTTCTAGGGTAAGGATTAAAACATTGCGCAATTTGCATTTGTGTTCCCTGGACAGGGCTCTCAAACCGTTGTAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTAGAAGAAACGTTTGCTGAAg                 >  2:352969/1‑140 (MQ=255)
                                                                                                                      cgcgCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAg                 >  1:2281813/1‑140 (MQ=255)
                                                                                                                       gcgcTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGc                <  1:975592/140‑1 (MQ=255)
                                                                                                                            ggTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTg           <  1:2539310/140‑1 (MQ=255)
                                                                                                                            ggTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTg           <  2:836730/140‑1 (MQ=255)
                                                                                                                              ttCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTgcg         >  1:1183902/1‑140 (MQ=255)
                                                                                                                                 gTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTgcggcg      <  1:1692413/140‑1 (MQ=255)
                                                                                                                                 gTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTgcggcg      <  1:1333791/140‑1 (MQ=255)
                                                                                                                                   ttCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCt    <  1:463875/140‑1 (MQ=255)
                                                                                                                                     cTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTgg  >  1:4545029/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGG  >  NZ_CP025268/1156677‑1156949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑