BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,212,832	A→G	61 (TAA→TAG) pseudogene (1/408 nt)	CXP41_RS06350 → CXP41_RS06355 →	protein YmfR phage portal protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,212,832	0	A	G	98.8%	1050.9 / NA	325	61 (TAA→TAG) pseudogene (1/408 nt)	CXP41_RS06350 CXP41_RS06355	protein YmfR phage portal protein
Reads supporting (aligned to +/- strand): ref base A (2/0); major base G (185/132); minor base T (3/1); total (190/135)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

CTTTTGCTGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACT  >  NZ_CP025268/1212695‑1212965

cTTTTGCTGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGGTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTg                                                                                                                                     >  1:307491/1‑140 (MQ=255)

  tttGCTGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGGGAGATATCTCGACCGTACACAGTGGTCTGTCGGCGGAGGTAAATAGTGtt                                                                                                                                   <  2:772106/140‑1 (MQ=255)

    tGCTGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCt                                                                                                                                 >  1:350884/1‑140 (MQ=255)

    tGCTGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCt                                                                                                                                 >  1:2736484/1‑140 (MQ=255)

       tGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCtttt                                                                                                                              <  1:3987381/140‑1 (MQ=255)

       tGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCtttt                                                                                                                              <  2:1482663/140‑1 (MQ=255)

                   ccTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGTCGCGATATATCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTc                                                                                                                  >  2:2509879/1‑140 (MQ=255)

                   ccTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTc                                                                                                                  >  1:553613/1‑140 (MQ=255)

                   ccTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTc                                                                                                                  >  1:199581/1‑140 (MQ=255)

                   ccTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTc                                                                                                                  >  1:1203608/1‑140 (MQ=255)

                   ccTGGCTGATTTATCCCCCGGCGGGTTTGGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTc                                                                                                                  >  1:290385/1‑140 (MQ=255)

                      ggCTGATTTATCCCCCGGCGGGGTGTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATCTCTCGGCCTTACACAGGCGTCTGTCGGCGGAGGTAAATAGTGTTCTGTTCGGGATGATTTCAAc                                                                                                               >  2:3959841/1‑140 (MQ=255)

                       gCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACg                                                                                                              <  1:4631961/140‑1 (MQ=255)

                        cTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGa                                                                                                             >  1:405284/1‑140 (MQ=255)

                          gATTTCTCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCCGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGaaa                                                                                                           <  2:1064075/140‑1 (MQ=255)

                           aTTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGaaaa                                                                                                          >  1:3254708/1‑140 (MQ=255)

                                 cccccGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGAc                                                                                                    <  1:3390068/140‑1 (MQ=255)

                                     cggcggGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCAc                                                                                                >  2:515538/1‑140 (MQ=255)

                                            ttttGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGAc                                                                                         <  2:1512518/140‑1 (MQ=255)

                                            tgttgttgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGAc                                                                                         <  2:505415/138‑1 (MQ=255)

                                                    ttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCcag                                                                                 <  1:266471/140‑1 (MQ=255)

                                                     tgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCcagc                                                                                >  2:3133585/1‑140 (MQ=255)

                                                      gCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGTTATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTTACCACGCcagca                                                                               <  2:2648386/140‑1 (MQ=255)

                                                      gCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTGGGCGGAGGTAAATAATGTTATTTTCGGGATTATTACAACGAAAAAGAGAAGCAACGGTGACCACGCaagca                                                                               >  2:4568359/1‑140 (MQ=255)

                                                      gCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCcagca                                                                               >  1:3614263/1‑140 (MQ=255)

                                                           ggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCt                                                                          >  1:2141961/1‑140 (MQ=255)

                                                             cgcTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTgg                                                                        <  1:2299120/140‑1 (MQ=255)

                                                               cTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCg                                                                      <  2:2401136/140‑1 (MQ=255)

                                                                 gtgCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGa                                                                    <  1:1751810/140‑1 (MQ=255)

                                                                      tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCa                                                               >  1:530431/1‑140 (MQ=255)

                                                                      tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCa                                                               <  1:1662785/140‑1 (MQ=255)

                                                                       gTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCAt                                                              >  2:2096746/1‑140 (MQ=255)

                                                                           tGGTCTTGGCTGTTGGCGCGATATCTCGTCCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTCCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCTCGCCAGCAGAGCTGGCGGATGCCATCggg                                                          <  2:791410/140‑1 (MQ=255)

                                                                           tGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                                          <  1:3133585/140‑1 (MQ=255)

                                                                           tGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                                          <  1:4471626/140‑1 (MQ=255)

                                                                            ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGCCGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                         <  2:348907/140‑1 (MQ=255)

                                                                            ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                         <  1:4375902/140‑1 (MQ=255)

                                                                            ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                         >  1:453713/1‑140 (MQ=255)

                                                                            ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                         <  1:2348449/140‑1 (MQ=255)

                                                                            ggTCGTGGCTGGTGGCGCGATATCTCGACCGGACACAGTCGTCTGTCGGCGGAGGTAAATAGGGTTCTTTGCGGGATTATTTCGACGAAAAAGTGACGCACCGGGGACCACGCCAGCAGAGCTGGCGGATGCCATCgggg                                                         >  2:4113923/1‑139 (MQ=255)

                                                                              tCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                                       <  2:2807135/140‑1 (MQ=255)

                                                                              tCGTGGCTGGTGGCGCCATATCTCGACCGTACCCAGTCGTTTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGCTTATTGCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGATCTGGCGGATGCCATCGGGCtc                                                       <  2:4234913/140‑2 (MQ=255)

                                                                              gcGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTTAACGAAACAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                                       <  2:3454708/139‑1 (MQ=255)

                                                                               cGTGGCTGGTGGGGCGATGTCTGGGCCGTACCCGGTCGTGTGTCGGGGGAGGTAAATAGTGTTCATTTCGGGCTTATTTCCAGGAAAAAGTGACGCACCGGAGACCACGCAAGCAGAGCTGGCGGATGCCATAGGGCTGt                                                      <  2:292507/140‑1 (MQ=255)

                                                                                gTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTc                                                     <  1:4278657/140‑1 (MQ=255)

                                                                                  ggCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACg                                                                                                   <  2:4391689/92‑1 (MQ=255)

                                                                                  ggCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGt                                                   >  2:2339855/1‑140 (MQ=255)

                                                                                  ggCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGt                                                   >  1:1143499/1‑140 (MQ=255)

                                                                                  ggCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGt                                                   >  1:956561/1‑140 (MQ=255)

                                                                                  ggCTGGTGGCGCGATATCTCGACCGTACACAGGCGGCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGGGACGCACCGGTGACCAAGCCCCCCATGCTGACGCATGCCCTCGCGCTGCCGt                                                   >  2:1833734/1‑140 (MQ=255)

                                                                                    cTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGTCCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                                 <  1:4811495/140‑1 (MQ=255)

                                                                                    cTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                                 <  2:302185/140‑1 (MQ=255)

                                                                                    cTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                                 <  1:2483110/140‑1 (MQ=255)

                                                                                       gtggCGCGATAGCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAAGAGTGTTCTTGTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGac                                              <  2:2750486/140‑1 (MQ=255)

                                                                                           cgcgATATCTCGACCGTACACAGTCGTTTGTCGGCGGAGGTAAATAGTTTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCTCCGGTGACCACGCCAGCAGAGCTGGCGGATGCCCTCGGGCTGTCATCTGACACCt                                          >  2:3733836/1‑140 (MQ=255)

                                                                                            gcgATACCTCCCCCGCACACAGTCGTCTGTCGGCTGAGGTAAATAGTGTTCTTCTCCTGACTATTTAAACTATAAAGTGACGCTCCGTGGACGACTCCAGCAGAGCTGGCGGATGCTTTCGGGCTGTCGTATGACACCta                                         <  2:3568650/140‑1 (MQ=255)

                                                                                             cgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCtat                                        >  2:551026/1‑140 (MQ=255)

                                                                                               atatCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATAc                                      <  2:4504043/140‑1 (MQ=255)

                                                                                                   ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGa                                  >  2:471627/1‑140 (MQ=255)

                                                                                                    tcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaa                                 <  1:732446/140‑1 (MQ=255)

                                                                                                    tcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaa                                 <  1:2853425/140‑1 (MQ=255)

                                                                                                    tcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTGTTCGGGAGTATGTCAACGAAAAAGTGACGCCCCGGTGACCGCGCCAGCATGGCGGGCGGATGCGATCGGGCTGGCGTCTGGCACCTATACCGGaa                                 >  2:2174706/1‑140 (MQ=255)

                                                                                                      gACCGTACACAGTCGTCTGTCGGCGGAGGTCAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAg                               <  1:3900009/140‑1 (MQ=255)

                                                                                                      gACCGTACACAGTCGTCTGTCGGCGGAGGTACATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAg                               <  1:2110898/140‑1 (MQ=255)

                                                                                                      gACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAg                               <  2:1630748/140‑1 (MQ=255)

                                                                                                       aCCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGc                              >  2:1797089/1‑140 (MQ=255)

                                                                                                       aCCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATGATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGc                              >  2:3950556/1‑140 (MQ=255)

                                                                                                          gTACACAGTCGTCTGGCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCTGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGa                           >  2:631929/1‑140 (MQ=255)

                                                                                                           tACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGACAGCAGAt                          >  2:129441/1‑140 (MQ=255)

                                                                                                           tACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGAt                          >  1:1558731/1‑140 (MQ=255)

                                                                                                            acacAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCtttt                                                                                                                              >  1:3650782/1‑39 (MQ=255)

                                                                                                            acacAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCtttt                                                                                                                              <  2:3650782/39‑1 (MQ=255)

                                                                                                            acacAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                         >  1:3800292/1‑140 (MQ=255)

                                                                                                            acacAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                         >  1:2990951/1‑140 (MQ=255)

                                                                                                            acacAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                         >  1:4995459/1‑140 (MQ=255)

                                                                                                               caGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATcagc                      <  1:2580465/140‑1 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCTGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:3089714/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAACGCAGATCAGCAGt                   >  2:3770380/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:2692717/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:873271/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  2:3196398/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:3861561/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:1281710/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  2:186570/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:1016872/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  1:19557/1‑140 (MQ=255)

                                                                                                                  tcgtcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGGGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGt                   >  2:3907634/1‑140 (MQ=255)

                                                                                                                     tcTGTCGGCGGCGGTAAAGATTGTTCTTTTCGCGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATTGGGCCGTCGTATGACATCTATACGGGAAAGCAGATCAGCAGTCat                >  2:1710952/1‑139 (MQ=255)

                                                                                                                     tcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAg                >  1:2746690/1‑140 (MQ=255)

                                                                                                                     tcTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAg                >  1:1425683/1‑140 (MQ=255)

                                                                                                                       tGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGGTTACTTCAACTAACAAGTTACGCACCGGTGACCACGCCAGCAGCGCTAGCTGAGGCTATCGGGCAGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg              <  2:4169765/140‑1 (MQ=255)

                                                                                                                       tGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGCTTATTTCAACGAAAAAGTGCCGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg              <  2:443566/140‑1 (MQ=255)

                                                                                                                       tGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg              >  2:4969025/1‑140 (MQ=255)

                                                                                                                       tGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg              <  1:2412864/140‑1 (MQ=255)

                                                                                                                       tGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg              >  1:4102039/1‑140 (MQ=255)

                                                                                                                       tGTCGGCCGAGGTGAATAGTGTTCTTTGCGGGAGTATTTCAACGAAAAAGTGACTCACCGGTGACCACGCCAGCAGAGCTGGCGGATCCCATCGGGTTGTCGTATGACTCCTATACCGGAAAGCAGATCTGCTGTCAGCg              <  2:4656186/140‑1 (MQ=255)

                                                                                                                            gcggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGAGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGCCACCGATACCGGAAAGCAGATCAGCAGTCAGCGGGCTa         >  2:92032/1‑140 (MQ=255)

                                                                                                                              ggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATg       >  2:2254271/1‑140 (MQ=255)

                                                                                                                                 ggTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGa    <  2:2010226/140‑1 (MQ=255)

                                                                                                                                 ggTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGa    >  1:4742405/1‑140 (MQ=255)

CTTTTGCTGGCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACT  >  NZ_CP025268/1212695‑1212965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`